Antibiotic susceptibility of microorganisms and related markers, compositions, methods and systems

ABSTRACT

Provided herein are RNA markers and compositions, methods and systems for the related identification and/or uses in methods for detection of antibiotic susceptibility and resistance in a microorganism, and in particular in N. gonorrhoeae.

CROSS REFERENCE TO RELATED APPLICATIONS

The present application is the U.S. National Stage of InternationalPatent Application No. PCT/US2019/044748, entitled “AntibioticSusceptibility of Microorganisms and Related Markers, Compositions,methods and Systems,” filed on Aug. 1, 2019 which claims priority toU.S. Provisional Application No. 62/713,412, entitled “AntibioticSusceptibility of Microorganisms and Related Markers, Compositions,methods and Systems” filed on Aug. 1, 2018 with docket number P2255-USP,the content of which is incorporated herein by reference in itsentirety.

STATEMENT OF GOVERNMENT GRANT

This invention was made with government support under Federal Award No.IDSEP160030-02awarded by the Department of Health and Human Services(HHS) Office of the Assistant Secretary for Preparedness and Response(ASPR) and the Wellcome Trust under the CARB-X program. The governmenthas certain rights in the invention.

FIELD

The present disclosure relates to microorganisms and related biology aswell as to diagnosis and treatment of related conditions in individuals.In particular, the present disclosure relates to antibioticsusceptibility of microorganisms and related markers, compositions,methods and systems.

BACKGROUND

Antibiotic susceptibility is an important feature of the biology ofvarious microorganisms, which can be used in identifying approaches totreat or prevent bacterial infections.

Ideal antibiotic therapy is based on determination of the etiologicalagent for a particular condition and determination of the antibioticsensitivity of the identified agent. In particular, the effectiveness ofindividual antibiotics varies with various factors including the abilityof the microorganism to resist or inactivate the antibiotic.

Despite progress in identifying methods and systems to test antibioticsusceptibility for various microorganisms, as well as the identificationof related markers, determination of antibiotic susceptibility can stillbe challenging. In particular, determination of antibioticsusceptibility when a rapid and accurate detection is desired formicroorganisms such as Neisseria gonorrhoeae which are slow growing andlack the classic transcriptional SOS response to DNA damage.

SUMMARY

Provided herein are RNA markers of antibiotic (sometimes abbreviated asABX) susceptibility of microorganisms and related compositions, methodsand systems that can be used for their identification and/or use. Inparticular described herein are RNA markers and related methods andsystems to test antibiotic susceptibility of microorganisms as well asRNA markers and related methods and systems for the diagnosis and/ortreatment of related infections in individuals.

According to a first aspect, a method is described to identify a RNAmarker of antibiotic susceptibility in a microorganism. The methodcomprises providing a susceptible isolate or specimen comprising astrain of the microorganism susceptible to the antibiotic and aresistant isolate or specimen comprising a strain of the microorganismresistant to the antibiotic.

The method further comprises providing a susceptible (Cs:Ts) value for acandidate marker gene in the susceptible isolate or specimen, wherein Csis a control susceptible gene expression value Cs for a candidate markerin a control susceptible sample not treated with the antibiotic and Tsis a treated susceptible gene expression for the candidate marker in atreated susceptible sample treated with the antibiotic.

The method also comprises providing a resistant (Cr:Tr) value for acandidate marker gene in the resistant isolate or specimen, wherein Cris a control resistant gene expression value for the candidate marker ina control resistant sample not treated with the antibiotic and Tr is atreated resistant gene expression for the candidate marker in a treatedresistant sample treated with the antibiotic. The method additionallycomprises selecting the candidate marker gene when Cs:Ts in thesusceptible isolate or specimen is different from Cr:Tr in the resistantisolate or specimen to provide a selected marker gene expressing the RNAmarker of antibiotic susceptibility of the microorganism. In particularthe selected marker gene is therefore differentially expressed in thetreated samples of the susceptible isolate or specimen compared with theresistant isolate or specimen as will be understood by a skilled person.

According to a second aspect, an RNA marker of antibiotic susceptibilityin a microorganism, a corresponding marker gene and/or a correspondingcDNA are described, which can be obtained by the method to identify anRNA marker of antibiotic susceptibility herein described.

In some embodiments the RNA marker can be selected from a transcriptencoding for a ribosomal protein of the microorganism. In some of thoseembodiments the RNA marker can be selected from a transcript encodingfor a 30S ribosomal protein and 50S ribosomal protein. In someembodiments, the RNA marker can be selected from: a transcript of N.gonorrhoeae gene having locus tag NGO0340, a transcript of N.gonorrhoeae gene having locus tag NGO1837, a transcript of N.gonorrhoeae gene having locus tag NGO1843, a transcript of N.gonorrhoeae gene having locus tag having locus tag NGO2024, a transcriptof N. gonorrhoeae gene having locus tag NGO1845, a transcript of N.gonorrhoeae gene having locus tag NGO1677, a transcript of N.gonorrhoeae gene having locus tag NGO1844, a transcript of N.gonorrhoeae gene having locus tag NGO0171, a transcript of N.gonorrhoeae gene having locus tag NGO1834, a transcript of N.gonorrhoeae gene having locus tag NGO0172, a transcript of N.gonorrhoeae gene having locus tag NGO1835, a transcript of N.gonorrhoeae gene having locus tag NGO1673, a transcript of N.gonorrhoeae gene having locus tag NGO1833, a transcript of N.gonorrhoeae gene having locus tag NGO2173, a transcript of N.gonorrhoeae gene having locus tag NGO0604, a transcript of N.gonorrhoeae gene having locus tag NGO0016, a transcript of N.gonorrhoeae gene having locus tag NGO1676, a transcript of N.gonorrhoeae gene having locus tag NGO1679, a transcript of N. genehaving locus tag NGO1658 and encoding hypothetical protein, a transcriptof N. gonorrhoeae gene having locus tag NGO1440, a transcript of N.gonorrhoeae gene having locus tag NGO0174, a transcript of N.gonorrhoeae gene having locus tag NGO0173, a transcript of N.gonorrhoeae gene having locus tag NGO0592, a transcript of N.gonorrhoeae gene having locus tag NGO1680, a transcript of N.gonorrhoeae gene having locus tag NGO0620, a transcript of N.gonorrhoeae gene having locus tag NGO1659, a transcript of N.gonorrhoeae gene having locus tag NGO1291, a transcript of N.gonorrhoeae gene having locus tag NGO0648, a transcript of N.gonorrhoeae gene having locus tag NGO0593, a transcript of N.gonorrhoeae gene having locus tag NGO1804, a transcript of N.gonorrhoeae gene having locus tag NGO0618, a transcript of N.gonorrhoeae gene having locus tag NGO0619, a transcript of N.gonorrhoeae gene having locus tag NGO1812, a transcript of N.gonorrhoeae gene having locus tag NGO1890, a transcript of N.gonorrhoeae gene having locus tag NGO2098, a transcript of N.gonorrhoeae gene having locus tag NGO2100 and a transcript tRNA havingGeneID A9Y61_RS02445 or NGO_t12, a tRNA transcript having GeneIDA9Y61_RS04515 or NGO_t15, a transcript tRNA having GeneID A9Y61_RS04510or NGO_t14, a transcript tRNA having GeneID A9Y61_RS09170 or NGO_t37, ora transcript tRNA having GeneID A9Y61_RS00075 or NGO_t01. The locus tagsand GeneIDs of the transcripts of N. gonorrhoeae gene are the locus tagsand GeneIDs of the registry of locus_tag prefixes of databases of theInternational Nucleotide Sequence Database Collaboration (INSDC) at thefiling date of the present disclosure.

According to a third aspect, a method is described to detect atranscript of an N. gonorrhoeae. The method comprises quantitativelydetecting in the N. gonorrhoeae a transcript expression value of an RNAmarker of N. gonorrhoeae selected from any one of the RNA markers of N.gonorrhoeae herein described, following contacting of the N. gonorrhoeaewith an antibiotic to obtain an antibiotic treated transcript expressionvalue for the RNA marker of N. gonorrhoeae

According to a fourth aspect, a method to perform an antibioticsusceptibility test for N. gonorrhoeae is described. The methodcomprises detecting susceptibility to an antibiotic of an N.gonorrhoeae, by quantitatively detecting in a sample comprising the N.gonorrhoeae a transcript expression value of an RNA marker of N.gonorrhoeae selected from the RNA markers of an N. gonorrhoeae hereindescribed following contacting the sample with the antibiotic.

According to a fifth aspect a method is described to detect an RNAmarker of susceptibility to an antibiotic in N. gonorrhoeae in a samplecomprising the N. gonorrhoeae. The method comprises contacting thesample with the antibiotic to obtain an antibiotic treated sample andquantitatively detecting in the antibiotic treated sample one or more ofthe RNA marker of N. gonorrhoeae herein described.

According to a sixth aspect, a method to diagnose susceptibility to anantibiotic of a N. gonorrhoeae infection in an individual is described.The method comprises contacting with the antibiotic a sample from theindividual comprising N. gonorrhoeae; and quantitatively detectingexpression by the N. gonorrhoeae in the sample of a marker of antibioticsusceptibility in N. gonorrhoeae selected from any one of thetranscripts of N. gonorrhoeae genes herein described. In the method, thequantitatively detecting is performed following or upon contacting thesample with the antibiotic. The method further comprises detectingwhether there is a downshift of the transcript presence quantitativelydetected in the antibiotic treated sample with respect to the transcriptpresence in a sample from the individual not treated with antibiotic andcomprising N. gonorrhoeae to diagnose the antibiotic susceptibility ofthe N. gonorrhoeae infection in the individual.

According to a seventh aspect, a method is described to detectantibiotic susceptibility of an N. gonorrhoeae bacterium and treat N.gonorrhoeae in an individual. The method comprises contacting a samplefrom the individual with an antibiotic, and quantitatively detecting inthe sample, expression by the N. gonorrhoeae bacteria of a marker ofantibiotic susceptibility selected from any one of the transcripts of N.gonorrhoeae genes herein described. In the method, the quantitativelydetecting is performed following contacting the sample with theantibiotic. The method further comprises diagnosing antibioticsusceptibility of N. gonorrhoeae infection in the individual when adownshift in expression of at least one of the detected markers in thesample is detected in comparison with a control untreated sample of theindividual. The method also comprises administering an effective amountof the antibiotic to the diagnosed individual.

According to an eighth aspect, a system is described for performing atleast one of the methods herein described to detect an N. gonorrhoeaetranscript, to detect antibiotic susceptibility of an N. gonorrhoeaebacteria, to perform an antibiotic susceptibility test for an Ngonorrhoeae, and/or to diagnose and/or treat an N. gonorrhoeae in anindividual. The system comprises at least one probe specific for atranscript selected from any one of the transcripts of N. gonorrhoeaegenes herein described or for a polynucleotide complementary thereof,and reagents for detecting the at least one probe.

In additional aspects, methods and systems are described, in which RNAmarkers and related marker genes and cDNAs of a microorganism other thanN. gonorrhoeae in accordance with the second aspect of the disclosure,are used in place of N. gonorrhoeae RNA markers and related genes andcDNA to: i) detect a transcript of the another microorganism, ii)perform an antibiotic susceptibility test for the another microorganism,detect an RNA marker of susceptibility to an antibiotic in the anothermicroorganism, diagnose susceptibility to an antibiotic of the anothermicroorganism infection in an individual, and/or detect antibioticsusceptibility of the another microorganism and treat the anothermicroorganism in an individual, the methods and systems comprising thefeatures according to the third to the eighth aspect of the instantdisclosure. In some of these embodiments the another microorganism is N.meningitidis.

RNA markers and related compositions methods and systems hereindescribed allow in several embodiments to elicit in a microorganism,(e.g. N gonorrhoeae) phenotypic responses to antibiotics that are fasterand greater in magnitude compared to responses in DNA markers.Therefore, in several embodiments RNA markers and related compositionsmethods and systems herein described allow phenotypic measurements ofantibiotic susceptibility and resistance of a microorganism (e.g. Ngonorrhoeae).

RNA markers and related compositions methods and systems hereindescribed allow in several embodiments to identify as markers ofantibiotic susceptibility responsive transcripts with the highestabundance and fold changes, as well as validated gene expression.

RNA markers and related compositions methods and systems hereindescribed allow in several embodiments to perform an accurate and rapidantibiotic susceptibility test for N. gonorrhoeae based on RNAsignatures.

RNA markers and related compositions methods and systems hereindescribed allow in several embodiments to compensate for errors insample splitting between treated and control samples and to compensatefor errors in sample preparation.

RNA markers and related compositions methods and systems hereindescribed can be used in connection with various applications whereinidentification and/or detection of antibiotic susceptibility for amicroorganism is desired, in particular when the microorganism is Ngonorrhoeae. For example, RNA markers and related compositions methodsand systems herein described can be used in drug research and to developdiagnostic and therapeutic approaches and tools to counteractinfections, in particular for N gonorrhoeae. Additional exemplaryapplications include uses of the RNA markers and related compositionsmethods and systems herein described in several fields including basicbiology research, applied biology, bio-engineering, aetiology, medicalresearch, medical diagnostics, therapeutics, and in additional fieldsidentifiable by a skilled person upon reading of the present disclosure.

The details of one or more embodiments of the disclosure are set forthin the accompanying drawings and the description below. Other features,objects, and advantages will be apparent from the description anddrawings, and from the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying drawings, which are incorporated into and constitute apart of this specification, illustrate one or more embodiments of thepresent disclosure and, together with the detailed description andexample sections, serve to explain the principles and implementations ofthe disclosure. Exemplary embodiments of the present disclosure willbecome more fully understood from the detailed description and theaccompanying drawings, wherein:

FIG. 1 illustrates an exemplary workflow for selection and validation ofRNA markers for phenotypic measurements of antibiotic susceptibility andresistance. Susceptible and resistant isolates of Neisseria gonorrhoeaeare exposed to antibiotics (ABX) for 5, 10, and 15 min. Samples arecollected for RNA sequencing at time zero and every 5 min thereafter.Genes demonstrating fold changes in expression (control:treated ratio(C:T ratio)) greater than the threshold of significance (edges of greyshaded area) are identified as differentially expressed (below greyshaded area: downregulated and above grey shaded area: upregulated).Candidate markers are selected from the pool of differentially expressedgenes and validated by digital PCR.

FIGS. 2A-B shows exemplary temporal shifts in global gene expressionupon ciprofloxacin exposure in Neisseria gonorrhoeae. FIG. 2A shows thedistribution of -log₂(C:T ratios) for a susceptible isolate (Sus) andresistant isolate (Res) at 0, 5, 10, and 15 min. FIG. 2B shows the foldchange in gene expression between control and treated samples (C:Tratio) versus expression in the control sample at 0, 5, 10, and 15 minfor one susceptible isolate and one resistant isolate. Genes with C:Tratios above or below the significance threshold are identified asdifferentially expressed (circles with diagonal lines: downregulated;solid black circles: upregulated). In the illustration of FIGS. 2A-B,thresholds for statistical significance of fold change (black solidlines) are determined by fitting a negative exponential curve (with 90%confidence interval) to the outer edge of the -log₂ C:T ratios measuredat time zero (see Methods).

FIGS. 3A-B show exemplary selection of candidate RNA markers forphenotypic antibiotic susceptibility testing in Neisseria gonorrhoeaeand measurements of marker abundances per cell. FIG. 3A shows genes thatare differentially expressed (dark grey) across three pairs of resistantand susceptible clinical isolates are identified as candidate markers(circles with vertical lines). Six candidate markers that span differentbiological functions were selected for validation (circles with diagonallines). FIG. 3B shows copies/cell values for the candidate markers aredetermined from RNA sequencing and dPCR (see Methods). Data are shownfor one pair of susceptible (S2) and resistant (R2) isolates at 15 minof ciprofloxacin exposure.

FIG. 4 shows an exemplary validation of the RNA sequencing approachusing digital PCR (dPCR) with six candidate markers. Control:Treatedratios (C:T ratios) determined by RNA sequencing (light gray) werevalidated against C:T ratios measured by dPCR (dark gray). The dPCR C:Tratios were normalized using ribosomal RNA (rRNA) by dividing the C:Tratio of marker by the C:T ratio of 16S rRNA. Markers were validatedusing two susceptible (S1 and S2) and two resistant (R1 and R2) isolatesat 15 min of ciprofloxacin exposure. In many sequencing experiments thecounts per gene result from sequencing of a random sampling of the RNApool. Relative expression values are calculated by normalizing to thetotal read count through generation of Transcript per Million (TPM)values (see Examples).

FIG. 5 shows in some embodiments antibiotic susceptibility testing of 49clinical isolates using (a) porB, and (b) rpmB as RNA AST markers.Antibiotic susceptibility of 49 clinical isolates (9 susceptible and 40resistant) from the Neisseria gonorrhoeae panel of the Central forDisease Control and Prevention (CDC) bacteria bank was determined usingthe “normalized” C:T ratios (C:T ratio of marker/C:T ratio of 16S rRNA).Clinical isolates were exposed to ciprofloxacin for 10 min and theconcentration of RNA markers was measured by digital PCR.

FIG. 6 shows a table containing a list of candidate markers and theirexpression in transcripts per million (TPM) and copies per cell forsusceptible isolate S2 and resistant isolate R2 after 15 min ofciprofloxacin exposure. The genome used for alignment was N. gonorrhoeaeFA1090 (NCBI Reference Sequence: NC_002946.2).

FIG. 7 shows a table containing exemplary primer sequences used forvalidation of candidate markers by digital PCR (SEQ ID NOs: 160-173).

FIG. 8 shows a table containing minimum inhibitory concentration (MIC)values for the 49 Neisseria gonorrhoeae clinical isolates acquired fromthe Center for Disease Control and Prevention (CDC) and Federal DrugAdministration (FDA) Antibiotic Resistance Isolate Bank published in2018.

FIG. 9 shows a diagram reporting a fitting a curve of the C:T ratiosexpected to be obtained at various antibiotic concentrations in aprophetic example of the methods and systems herein described. Inparticular in the diagram of FIG. 9 , the CT ratios obtained for aparticular sample are reported vs the related concentration ofantibiotic for samples comprising a microorganism susceptible to theantibiotic (black circles) a microorganism having intermediatesusceptibility to the antibiotic (black squares) and a microorganismresistant to the antibiotic (black triangles). In the propheticillustration of FIG. 9 , the microorganism is N. gonorrhoeae and theantibiotic is ciprofloxacin.

ANNEX A-E

The accompanying ANNEX A provides exemplary 16S rRNA and 23S rRNAsequences (SEQ ID NO: 1-9 and 13-27) that can be used as controltranscript for normalization. ANNEX B provides exemplary marker genes(SEQ ID NO: 28-153 and 228-230) differentially expressed by an exemplarymicroorganism (N. gonorrhoeae) in an untreated sample and in a sampletreated with an antibiotic. ANNEX C provides exemplary marker genes (SEQID NO: 154-159) expected to be differentially expressed by an exemplarymicroorganism (N. meningitidis) in an untreated sample and in a sampletreated with an antibiotic. ANNEX D provides sequences of an exemplarymarker of antibiotic susceptibility (porB) in 50 clinical isolates fromthe Center of Disease Control and Prevention (CDC) bank (SEQ ID NO:178-227). ANNEX E provides a list of exemplary RNAs reported in Table 1(SEQ ID NO: 231-344 and SEQ ID NO: 10-12) with a log2 fold change lessthan 0.32 (corresponding to <25% change) that can be used as controltranscripts. ANNEX A to E which are incorporated into and constitute apart of this specification, together with the detailed descriptionsection, serve to explain the principles and implementations of thedisclosure. Other features, objects, and advantages will be apparentfrom the entire description and drawings, and from the claims.

DETAILED DESCRIPTION

Provided herein are RNA markers of antibiotic susceptibility ofmicroorganisms and related compositions, methods and systems for theiridentification and/or use.

The term “RNA” or “Ribonucleic acid” as used herein indicates apolynucleotide composed of our of ribonucleotide bases: or an analogthereof linked to form an organic polymer. The term “ribonucleotide”refers to any compounds that consist of a ribose (ribonucleotide) sugarjoined to a purine or pyrimidine base and to a phosphate group, and thatare the basic structural units of a ribonucleic acid, typically adenine(A), cytosine (C), guanine (G), and uracil (U). In an RNA adjacentribose nucleotide bases are chemically attached to one another in achain typically via phosphodiester bonds. The term “ribonucleotideanalog” refers to a ribonucleotide in which one or more individual atomshave been replaced with a different atom with a different functionalgroup. For example, ribonucleotide analogues include chemically modifiedribonucleotides, such as methylation hydroxymethylation glycosylationand additional modifications identifiable by a skilled person. Examplesof chemical modifications of RNA comprise dynamic modifications to RNAidentified in the transcriptome, including N⁶-methyladenosine (m⁶A),inosine (I), 5-methylcytosine (m⁵C), pseudouridine (Ψ),5-hydroxymethylcytosine (hm⁵C), and N¹-methyladenosine (m¹A), andrelated epitranscriptome which are described in Song and Yi 2017,. [1]Additional chemical modifications of transfer RNA (tRNA) are describedin Jackman and Alfonzo 2013 [2] (Accordingly, the term RNA includesribonucleic acids of any length including analogs or fragments thereof.

The term “marker” as used herein refers to a category of characteristicsthat are objectively measured and evaluated as an indicator ofbiological processes, pathogenic processes, or pharmacologic response toa therapeutic intervention or an environmental exposure. A marker can beany molecule associated with the process and/or response of interest andthat can be used as an identifier to detect the process and/or responseof interest, such as certain characteristics in a microorganism and/orits response to a therapeutic intervention or an environmental exposureincluding exposure to antibiotics.

The term “antibiotic” sometimes abbreviated as ABX, as used hereinrefers to a type of antimicrobial used in the treatment and preventionof bacterial infection. Some antibiotics can either kill or inhibit thegrowth of bacteria. Others can be effective against fungi andprotozoans. The term “antibiotic” can be used to refer to any substanceused against microbes. Antibiotics are commonly classified based ontheir mechanism of action, chemical structure, or spectrum of activity.Most antibiotics target bacterial functions or growth processes.Antibiotics having bactericidal activities target the bacterial cellwall, such as penicillins and cephalosporins, or target the cellmembrane, such as polymyxins, or interfere with essential bacterialenzymes, such as rifamycins, lipiarmycins, quinolones and sulfonamides.Antibiotics having bacteriostatic properties target protein synthesis,such as macrolides, lincosamides and tetracyclines. Antibiotics can befurther categorized based on their target specificity. “Narrow-spectrum”antibacterial antibiotics target specific types of bacteria, such asGram-negative or Gram-positive bacteria. “Broad-spectrum” antibioticsaffect a wide range of bacteria. Exemplary antibiotics comprisetopoisomerase inhibitors which are chemical compounds capable ofblocking the action of a topoisomerase such as topoisomerase I and II (atype of enzyme that controls the changes in DNA structure by catalyzingthe breaking and rejoining of the phosphodiester backbone of DNA strandsduring the normal cell cycle) and fluoroquinolones which are quinolonescontaining a fluorine atom in their chemical structure and are effectiveagainst both Gram-negative and Gram-positive bacteria. A quinoloneantibiotic indicates any member of a large group of broad-spectrumbactericides that share a bicyclic core structure related to thecompound 4-quinolone. Exemplary fluoroquinolones include ciprofloxacin(Cipro), gemifloxacin (Factive), levofloxacin (Levaquin), moxifloxacin(Avelox), norfloxacin (Noroxin), and ofloxacin (Floxin).

The wording “antibiotic susceptibility” or “antibiotic sensitivity” asused herein indicates the susceptibility of bacteria to antibiotics andthe antibiotic susceptibility can vary within a species. Antibioticsusceptibility testing (AST) can be carried out to predict the clinicalresponse to treatment and guide the selection of antibiotics as will beunderstood by a person skilled in the art. In some embodiments, ASTcategorizes organisms as susceptible, resistant, or intermediate to acertain antibiotic.

Microorganisms can be classified as susceptible (sensitive),intermediate or resistant based on breakpoint minimum inhibitoryconcentration (MIC) values that are arbitrarily defined and reflect theachievable levels of the antibiotic, the distribution of MICs for theorganism and their correlation with clinical outcome. MIC value of amicroorganism is the lowest concentration of an antibiotic that willinhibit its growth. Methods that can be used to measure the MIC of amicroorganism comprise broth dilution, agar dilution and gradientdiffusion (the ‘E test’), where twofold serial dilutions of antibioticare incorporated into tubes of broth, agar plates or on a paper strip,respectively, as will be understood by a person skilled in the art. Thedisk diffusion method defines an organism as susceptible or resistantbased on the extent of its growth around an antibiotic-containing disk.MIC values are influenced by several laboratory factors.

Laboratories follow standard for parameters such as incubationtemperature, incubation environment, growth media, as well as inoculumand quality control parameters. In the U.S., standards for performingAST as well as breakpoint MIC values for various bacteria can be foundin Clinical & Laboratory Standards Institute (CLSI) publications (seethe web pagehttps://clsi.org/standards/products/microbiology/documents/m100/ at thedate of filing of the present disclosure). An example of standards forperforming an Antibiotic Susceptibility Test (AST) as well as breakpointMIC values for various bacteria which can be used in embodiments of thepresent disclosure is provided in Example 16. In Europe, standards forperforming AST as well as breakpoint MIC values for bacteria can befound in European Committee on Antimicrobial Susceptibility Testing(EUCAST) see http://www.eucast.org/clinical_breakpoints/ at the time offiling of the instant disclosure) as will be understood by the skilledperson.

The term “microorganism”, or “microbe” as used herein indicates amicroscopic organism, which may exist in its single-celled form or in acolony of cells, such as prokaryotes and in particular bacteria.

The term “prokaryotic” is used herein interchangeably with the terms“cell” and refers to a microbial species which contains no nucleus orother organelles in the cell. Exemplary prokaryotic cells includebacteria.

The term “bacteria” or “bacterial cell”, used herein interchangeablywith the terms “cell” indicates a large domain of prokaryoticmicroorganisms. Typically a few micrometers in length, bacteria have anumber of shapes, ranging from spheres to rods and spirals, and arepresent in several habitats, such as soil, water, acidic hot springs,radioactive waste, the deep portions of Earth’s crust, as well as insymbiotic and parasitic relationships with plants and animals. Bacteriain the sense of the disclosure refers to several prokaryotic microbialspecies which comprise Gram-negative bacteria Gram-positive bacteria,Proteobacteria, Cyanobacteria, Spirochetes and related species,Planctomyces, Bacteroides, Flavobacteria, Chlamydia, Green sulfurbacteria, Green non-sulfur bacteria including anaerobic phototrophs,Radioresistant micrococci and related species, Thermotoga andThermosipho thermophiles as would be understood by a skilled person.More specifically, the wording “Gram positive bacteria” refers to cocci,nonsporulating rods and sporulating rods, such as, for example,Actinomyces, Bacillus, Clostridium, Corynebacterium, Erysipelothrix,Lactobacillus, Listeria, Mycobacterium, Myxococcus, Nocardia,Staphylococcus, Streptococcus and Streptomyces.

The term “proteobacteria” as used herein refers to a major phylum ofGram-negative bacteria. Many move about using flagella, but some arenonmotile or rely on bacterial gliding. As understood by skilledpersons, taxonomic classification as proteobacteria is determinedprimarily in terms of ribosomal RNA (rRNA) sequences. The Proteobacteriaare divided into six classes, referred to by the Greek letters alphathrough epsilon and the Acidithiobacillia and Oligoflexia, includingalphaproteobacteria, betaproteobacteria and gammaproteobacteria as willbe understood by a skilled person. Proteobacteria comprise the species:N. gonorrhoeae and N meningitidis within the class ofBetaproteobacteria, the order: Neisseriales the Family of Neisseriaceaeand the Genus of Neisseria.

In embodiments of the instant disclosure, RNA markers are described andrelated methods and systems to test antibiotic susceptibility ofmicroorganisms as well as for the diagnosis and/or treatment of relatedinfections in individuals.

In particular, in some embodiments described herein is a method toidentify an RNA marker of antibiotic susceptibility in a microorganism.The method herein described is based on the use of a susceptible isolateor specimen comprising a strain of the microorganism susceptible to theantibiotic and of a resistant isolate or specimen comprising a strain ofthe microorganism resistant to the antibiotic.

The term “isolate” as used herein indicates a portion of matterresulting from a separation of a strain of a microorganism from anatural, usually mixed population of living microbes, as present in anatural or experimental environment, for example in water or soil flora,or from living beings with skin flora, oral flora or gut flora.

The word “specimen” as used herein indicates a portion of matter from anenvironment for use in testing, examination, or study. The environmentcan comprise living beings and in particular human beings. In theseinstances a specimen can include portion of tissues, organs or otherbiological material from the living being such as urethra, urine,cervix, vagina, rectum, oropharynges, conjunctiva, or any body fluids.

In some embodiments, the isolates can be obtained from isolate bankssuch as CDC and FDA AR Isolate Bank which provide curated collections ofsusceptible and resistant organisms. In particular in embodimentswherein the microorganism is N. gonorrhoeae, the susceptible andresistant isolates are obtained from the N. gonorrhoeae panel of the CDCAntimicrobial Resistance Isolate Bank, which as of Aug. 1, 2018contained 50 total isolates.

In methods to identify such an RNA marker of antibiotic susceptibilityin a microorganism herein described, the selected RNA marker ofantibiotic susceptibility identified by the method is a transcript of agene which is differentially expressed in a sample of the susceptibleisolate or specimen treated with the antibiotic and in sample of theresistant isolate or specimen treated with the antibiotic.

The term “sample” as used herein indicates a limited quantity ofsomething that is indicative of a larger quantity of that something,including but not limited to fluids from an isolate or a specimen suchas biological environment, cultures, tissues, commercial recombinantproteins, synthetic compounds or portions thereof. In particularbiological sample can comprise one or more cells of any biologicallineage, as being representative of the total population of similarcells in the sampled individual. Exemplary biological samples comprisethe following: cheek tissue, whole blood, dried blood spots, organtissue, plasma, urine, mucus, mucosal secretions, vaginal fluids andsecretions, urethral fluids and secretions, feces, skin, hair, or tumorcells, among others identifiable by a skilled person. Biological samplescan be obtained using sterile techniques or non-sterile techniques, asappropriate for the sample type, as identifiable by persons skilled inthe art. Some biological samples can be obtained by contacting a swabwith a surface on a human body and removing some material from saidsurface, examples include throat swab, urethral swab, oropharyngealswab, cervical swab, vaginal swab, genital swab, anal swab. Depending onthe type of biological sample and the intended analysis, biologicalsamples can be used freshly for sample preparation and analysis, or canbe fixed using fixative. Preferably, in methods and systems hereindescribed the sample comprises live cells.

The wording “differentially expressed” as used herein with respect to agene indicates a difference in the expression of the gene by a cellunder different experimental, environmental and/or biologicalconditions. Accordingly, differential expression of a gene can bedetected in a microorganism following a different in one or more ofthese conditions as will be understood by a skilled person. For example,the wording “differentially expressed” can reference to a difference inthe expression of a gene in a microorganism: i) with or without drugtreatment, ii) on a same sample or different samples, and/or iii) atdifferent times. Accordingly, differential expression analysis requiresthat gene expression values detected under the different conditions becompared and therefore that the expression of the genes bequantitatively detected.

In particular, detection of a differential expression of a gene in asusceptible or resistant isolate or specimen according to methods hereindescribed can be performed by quantitatively detecting the expression ofthe gene in samples of the susceptible and resistant isolate orspecimen.

The terms “detect” or “detection” as used herein indicates thedetermination of the existence, presence or fact of a target in alimited portion of space, including but not limited to a sample, areaction mixture, a molecular complex and a substrate. The “detect” or“detection” as used herein can comprise determination of chemical and/orbiological properties of the target, including but not limited toability to interact, and in particular bind, other compounds, ability toactivate another compound and additional properties identifiable by askilled person upon reading of the present disclosure. The detection canbe quantitative or qualitative. A detection is “quantitative” when itrefers, relates to, or involves the measurement of quantity or amount ofthe target or signal (also referred as quantitation), which includes butis not limited to any analysis designed to determine the amounts orproportions of the target or signal. A detection is “qualitative” whenit refers, relates to, or involves identification of a quality or kindof the target or signal in terms of relative abundance to another targetor signal, which is not quantified.

An exemplary way to quantitatively detect differential expression is thefold change approach which can be used as a criterion to selectdifferentially expressed genes as will be understood by a person skilledin the art. In the fold-change approach, a gene is considered to bedifferentially expressed if the ratio of the normalized markerexpression level, possibly normalized, between the antibiotic treatedand untreated conditions exceeds a certain threshold

In methods herein described, quantitative detection of expression of agene can be performed with various techniques such as by RNA-seq, qPCR,digital PCR, and isothermal techniques such as LAMP or digitalisothermal, microarrays signals, Nanostring as well high throughput RNAsequencing as reads per kilobase per million reads (RPKM) or transcriptsper million (TPM) for RNA-seq data and additional nucleic acidquantification techniques identifiable to a skilled person. It should beunderstood that in such methods quantitative detection of expression ofa gene is commonly combined with a reverse transcription step to convertthe RNA sequence into a cDNA sequence which can be quantified by methodsdescribed herein and/or identifiable by a skilled person. Eithersequence-specific or sequence-non-specific primers can be used toinitiate reverse transcription of a target gene as will be understood bya skilled person.

In some embodiments, detecting specific gene expression can be performedat the transcription level by performing RNA-seq and calculating RNAexpression values based on the sequence data.

In some embodiments, the RNA expression values can be detected andprovided as transcripts per million (TPM) as will be understood by aperson skilled in the art. In particular, to calculate TPM, read countsare first divided by the length of each gene in kilobases, which givesreads per kilobase (RPK). RPKs for all genes are added and the sum isdivided by 1,000,000. This gives the “per million” scaling factor.Finally, the RPK value for each genes is divided by the “per million”scaling factor to give TPM. [3]

In particular, in method to identify an RNA marker of antibioticsusceptibility herein described, quantitatively detecting the expressionof a gene is performed in treated samples of the susceptible andresistant isolate or specimen following treatment of the samples withthe antibiotic and in control samples of the susceptible and resistantisolate or specimen without treatment with the antibiotic.

In some of these embodiments, providing a treated sample and a controlsample of the susceptible and/or resistant isolate or specimen cancomprise contacting a first sample of the susceptible and/or resistantisolate or specimen with a treatment media to obtain the susceptibleand/or resistant control samples respectively and contacting a secondsample of the susceptible and/or resistant isolate or specimen from thesame source or host with the same treatment media and an antibiotic toobtain a susceptible and/or resistant antibiotic treated samplerespectively. The contacting time (referring to the duration of thecontact) with the treatment media is preferably substantially the samefor the control sample and the treated sample. The wording“substantially the same” when referred to two or more times indicatestimes differing one from another of an amount up to 30%, Accordingly,for example two contacting times are substantially the same in the senseof the disclosure, if they are within approximately 30% of each other,20% of each other, 10% of each other, 5% of each other. For example, thetwo contacting times can be within 2 minutes of each other, or within 1minute of each other.

In some particular embodiments, treatment of a sample with a treatmentmedia is performed to create a controlled environment that wouldminimize the impact of biochemical parameters of a sample, such as pH orsalt concentration or presence of molecules other than RNA or cells(human cells or other microorganisms other than target microorganismfrom which gene expression is to be detected)) on the gene expressionand RNA response of the target microorganism to an external stimulussuch as a antibiotic treatment and/or quantitative detection of geneexpression. Treatment media can be used to create a more controlledenvironment for obtaining a more reliable gene expression. For example,treatment media can be composed of commercially available brothsdesigned for the cultivation of microorganisms (such as Fastidious Brothfrom Hardy Diagnostics) or prepared using chemically defined components.In some cases, commercial broths can be diluted to create the desiredtreatment environment. For example, a specific osmolarity (for examplein the range 0.0 - 0.5 osmols) or pH (for example in the range 5 - 9).Treatment media can be modified to contain specific factors to increaseor decrease the metabolism of the target microorganism (such as carbonsource or specific anions or cations). Gentle or vigorous mixing can beperformed at specific time intervals after the addition ofmicroorganisms to the treatment media in order to maintain homogeneityand reliable gene expression.

In some embodiments, a control sample and/or treated sample of thesusceptible and/or resistant isolate or specimen can preferably bepretreated to enrich said sample with RNA or with the targetmicroorganism, and/or to remove human RNA or RNA of othermicroorganisms. The removal of human RNA can be performed viahybridization to beads or columns with probes specific for human RNA.The removal of human RNA can also be performed via selective lysis ofhuman cells and degradation of released human RNA. The sample may alsobe pretreated to enrich or deplete, as desired, tRNA via size selection.

In some embodiments, treatment or exposure with antibiotic can beperformed by adding antibiotics to the microorganism and incubating thesample under certain condition preferably following and/or uponcontacting the sample with a treatment media.

Treatment media used in connection with antibiotic exposure inaccordance to methods herein described can be designed to supportphysiological processes of the target microorganism, enable oraccelerate DNA replication and translation, maintain cellular uniformityand homogeneity in suspension, and promote interaction of themicroorganism and antibiotic. Accordingly, the treatment media can beselected to include a source of energy and nourishment specific for thetarget microorganism, such as carbon, hydrogen, oxygen, nitrogenphosphorus, Sulphur, potassium, magnesium, calcium, iron, trace elementsand organic growth factors which can be provided as organic sources suchas simple sugars e.g. glucose, acetate or pyruvate, amino acids,nitrogenous bases or extracts such as peptone, tryptone, yeast extractand additional identifiable by a skilled person., Inorganic sources suchas ; carbon dioxide (CO2) or hydrogen carbonate salts (HCO3)NH4CI,(NH4)2S04, KNO3, and for dinitrogen fixers N2, KH2PO4, Na2HPO4, Na2SO4,H2S, KCI, K2HPO4, MgCI2, MgSO4, CaCI2, Ca(HC03)2, NaCI, FeCI3,Fe(NH4)(SO4)2, Fe-chelates1), CoCI2, ZnCI2, Na2MoO4, CuCI2, MnSO4,NiCI2, Na2SeO4, Na2WO4, Na2VO4, as well as Vitamins, amino acids,purines, pyrimidines (see the websitehttps://www.sigmaaldrich.com/technical-documents/articles/microbiology/microbiology-introduction.htmlat the filing date of the present disclosure). Additional parametersconsidered to select the proper treatment media for a targetmicroorganism comprise osmotic pressure, pH, oxygen content, watercontent, carbon dioxide content as will be understood by a skilledperson to support physiological processes of the target microorganism,enable or accelerate DNA replication and translation, maintain cellularuniformity and homogeneity in suspension, and promote interaction of themicroorganism and antibiotic. For example in the experiments describedherein with reference to N. gonorrhoeae the treatment media used wasFastidious Broth from Hardy Diagnostics (cat no. K31) which comprisepancreatic Digest of Casein , Yeast Extract, Dextrose, Peptic Digest ofAnimal Tissue, Sodium Chloride, Brain Heart Infusion, TRIS , PancreaticDigest of Gelatin, Agarose, L-Cysteine HCl, Magnesium Sulfate, FerrousSulfate , Hematin, NAD, Pyridoxal and Tween® 80 (seehttps://catalog.hardydiagnostics.com/cp_prod/content/hugo/fbbroth.htm atthe filing date of the present disclosure) Additional treatment mediasuitable to support physiological processes of N. gonorrhoeae, to enableor accelerate DNA replication and translation, maintain cellularuniformity and homogeneity in suspension, and promote interaction of theN. gonorrhoeae and the antibiotic are identifiable by a skilled person.

In methods herein described, incubation of a sample with an antibioticcan be performed at a temperature such that a physiological response tothe antibiotic is generated in the target microorganism (often themicroorganisms optimal growth temperature, for example 37° C. or at atemperature ± 0.5 degrees, ± 1 degree, ± 2 degrees, ± 3° C. therefrom).Also, adding the antibiotics can be performed throughout incubation orat set intervals during incubation to increase or decrease thephysiological response of the microorganism to the antibiotic.

In particular in some embodiments, the antibiotic for treating thesample herein described can be provided at a concentration equal to orabove the breakpoint MIC for the susceptible isolate or specimen to theantibiotic. In particular, the antibiotic for treating the sample hereindescribed can be provided at a concentration lower than the breakpointMIC for the resistant isolate or specimen to the antibiotic, for example1.5 times (or 1.5X) lower, 2 times (or 2X) lower, 3 times (or 3X) lower,4 times (or 4X) lower, 8 times (or 8X) lower, or 16 times (or 16X) lowerthan the breakpoint MIC for a resistant isolate. In some embodiments,the antibiotic for treating the sample herein described is provided at aconcentration higher than the breakpoint MIC for the resistant isolateor specimen to the antibiotic, for example 1.5 times (or 1.5X) higher, 2times (or 2X) higher, 3 times (or 3X) higher, or 4 times (or 4X) higher,8 times higher (8X), 16 times higher (or 16X) than then breakpoint MIC.The breakpoint MIC of the antibiotic can be obtained from the Clinical &Laboratory Standards Institute (CLSI) guidelines, European Committee ofAntimicrobial Susceptibility Testing (EUCAST) or other sourcesidentifiable to a skilled person. In some embodiments, samples can betreated at several concentrations of the antibiotics for example, tomeasure the MIC of an organism before identifying the marker ofantibiotic susceptibility as will be understood by a skilled person.

In some embodiments, antibiotic treatment or exposure can be performedfor a set time period (e.g. up to 5 minutes, 10 minutes, 15 minutes or20 minutes or any other time between 0-20 minutes or longer).

In some embodiments of the methods of the instant disclosure, the timeperiod of contacting the sample with an antibiotic is shorter than thedoubling time of the target organism. For example, the time ofcontacting could be less than 1x doubling time, less than 0.75X doublingtime, less than 0.5 doubling time, less than 0.35 doubling time, lessthan 0.25 doubling time, less than 0.2 doubling time, less than 0.15doubling time, less than 0.1 doubling time, less than 0.075 doublingtime, less than 0.05 doubling time.

During the incubation, the sample can be collected at different timeinterval for further analysis (see Example 1). In addition to collectingsamples during the incubation with antibiotics, samples can be collectedfor analysis before treatment or exposure. Such samples can be used ascontrols in analysis. Detection of response of the microorganism to theantibiotic can be performed one or more times at any time afterantibiotic treatment or exposure. In some embodiments, rapid detection,for example detection completed within 10 minutes, 15 minutes, 20minutes, 30 minutes, 40 minutes after exposure.

In some of embodiments of the method to identify an RNA marker ofantibiotic susceptibility herein described, providing a treated sampleand a control sample of the susceptible and/or resistant isolate orspecimen can comprise enriching a first sample and a second sample ofthe susceptible and/or resistant isolate or specimen from the samesource or host with the microorganism to obtain the susceptible and/orresistant control samples respectively, and contacting the second samplewith an antibiotic to obtain a susceptible and/or resistant antibiotictreated sample respectively.

In embodiments of the method to identify an RNA marker of antibioticsusceptibility herein described,, providing a treated sample and acontrol sample of the susceptible and/or resistant isolate or specimencan comprise enriching a first sample and a second sample of thesusceptible and/or resistant isolate or specimen from the same source orhost with the microorganism, contacting the first sample with atreatment media following the enriching to obtain the susceptible and/orresistant control samples respectively and contacting the second sampleof the susceptible and/or resistant isolate or specimen from the samesource or host with the same treatment media and an antibiotic to obtaina susceptible and/or resistant antibiotic treated sample respectively.

In methods herein described, enriching a sample with the microorganismscan be performed between sample collection (and optionally elution froma collection tool such as a swab) and exposure. In particular enrichinga sample with microorganisms and in particular bacteria (such asNeisseria gonorrhoeae) can be performed by capturing the microorganismusing a solid support (e.g. a membrane, a filtration membrane, anaffinity membrane, an affinity column) or a suspension of a solidreagent (e.g. microspheres, beads). Capture of a target microorganismcan improve the assay and the response to antibiotic. Capture can beused to enrich/concentrate low-concentration samples. Capture followedby washing can be used to remove inhibitors or components that mayinterfere with the method described here. Capture followed by washingmay be used to remove inhibitors of nucleic acid amplification orinhibitors of other quantitative detection assays. Enrichment can alsobe performed using lysis-filtration techniques to lyse host cells anddissolve protein and/or salt precipitates while maintaining bacterialcell integrity then capturing target bacteria on filters (e.g. mixedcellulose ester membranes, polypropylene and polysulfone membranes).Enrichment can also be performed by binding target bacteria to membranesof microspheres, optionally coated with an affinity reagent (e.g. anantibody, an aptamer) specific to the target bacteria’s cell envelope.When microspheres or beads are used for capture, they can be filtered,centrifuged, or collected using a magnet to enrich bacteria. AST in theformat described here can then be performed directly on capturedbacteria, or the bacteria can be released before performing the method.

Accordingly, in methods to identify an RNA marker of antibioticsusceptibility, quantitative detection of a marker gene is performed toprovide for each of the detected genes a control gene expression value Cin a control sample not treated with the antibiotic and a correspondingtreated gene expression value T in a treated sample treated with theantibiotic in each of the susceptible and resistant isolate or specimen.

In particular, quantitative detection of the expression of one or moregenes in method herein described to identify an RNA marker of antibioticsusceptibility is performed to provide

-   a control susceptible gene expression value Cs for each of the    detected genes in a control susceptible sample not treated with the    antibiotic and a corresponding treated susceptible gene expression    Ts for each of the detected genes in a treated susceptible sample    treated with the antibiotic; and-   a control resistant gene expression value Cr for each of the    detected genes in a control resistant sample not treated with the    antibiotic and a corresponding treated resistant gene expression Tr    for each of the detected genes in a treated resistant sample treated    with the antibiotic.

More particularly in methods to identify an RNA marker of antibioticquantitative detection of the expression of one or more genes isperformed to provide a susceptible (Cs:Ts) value for a candidate markergene in the susceptible isolate or specimen, and a resistant (Cr:Tr)value for a candidate marker gene in the resistance isolate or specimen.

In particular providing a susceptible (Cs:Ts) value for the candidatemarker gene in the susceptible isolate or specimen can be performed by

-   providing a treated susceptible sample treated with the antibiotic    and a control susceptible sample not treated with the antibiotic,-   quantitatively detecting a control susceptible gene expression value    Cs for a candidate marker gene in the control susceptible sample,-   quantitatively detecting a treated susceptible gene expression value    Ts for the candidate marker gene in the treated susceptible sample,    and-   providing a susceptible (Cs:Ts) value for the candidate marker gene    by dividing Cs for the candidate marker gene by Ts for the candidate    marker gene .

Additionally, providing a resistant (Cr:Tr) value for the candidatemarker gene in the at least one resistant isolate or specimen can beperformed by.

-   providing a treated resistant sample treated with the antibiotic and    a control resistant sample not treated with the antibiotic,-   quantitatively detecting a control resistant gene expression value    Cr for the candidate marker gene in the control resistant sample,-   quantitatively detecting a treated resistant gene expression value    Tr for the candidate marker gene in the treated resistant sample,    and-   providing a resistant (Cr:Tr) value for the candidate marker gene by    dividing Cr for the candidate marker gene by Tr for the candidate    marker gene.

In methods to identify an RNA marker of antibiotic susceptibility, theRNA is identified by selecting the candidate marker gene when Cs:Ts isdifferent from Cr:Tr to provide a selected marker gene differentiallyexpressed in the treated susceptible sample and in the treated resistantsample.

In some embodiments, the Cs:Ts ratio and the Cr:Tr ratios are providedby gene expressionin TPM in the control sample divided by the geneexpression in TPM in the treated sample.

In some embodiments, the Cs:Ts ratio and the Cr:Tr ratios can beprovided by RPKM (reads per kilobase per million mapped reads). The useof RPKM and comparison to TPM is described for example in Wagner et al2012 [3]. In some embodiments the Cs:Ts ratio and the Cr:Tr ratios areprovided by FPKM (fragments per kilobase per million), the use of FPKMis described for example in Conesa, Ana, et al. 2016 [4]. These unitsnormalize for sequencing depth and transcript length. In someembodiments RPM (reads per million mapped reads; RPM does not normalizefor transcript length) or raw sequencing read counts can be used.Typically, to calculate RPM (reads per million), the total reads from asample are divided by 1,000,000 to obtain the “per million scalingfactor”. The read counts for each gene are then divided by the “permillion scaling factor” to give RPM. Also typically to calculate RPKM(for single-end RNA-seq), the RPM values are divided by the gene lengthin kilobases. FPKM (for paired-end RNA-seq), is calculated the same wayas RPKM, taking into account that with paired-end RNA-seq, two reads cancorrespond to a single fragment, or, if one read in the pair did notmap, one read can correspond to a single fragment as will be understoodby a skilled person.

In some embodiments, the Cs:Ts ratio and the Cr:Tr ratio can be plottedas -log₂(C:T) against the -log₂(expression in TPM) for all genes (FIGS.1-3 ).

In some embodiments, to qualify for a marker gene differentiallyexpressed in the treated sample of the susceptible isolate or specimenand in the treated sample of the resistant isolate or specimen, thedifference between the (Cs:Ts) value and resistant (Cr:Tr) value isstatistically significant.

In preferred embodiments, to qualify for a marker gene differentiallyexpressed in the treated sample of the susceptible isolate or specimenand in the treated sample of the resistant isolate or specimen, thedifference between the (Cs:Ts) value and resistant (Cr:Tr) value isstatistically significant over the related biological variability(variability due to physiologic differences among a biological unit of asame microorganism such as between different strains of themicroorganism and/or between different individual microorganism of asame strains) and/or technical variability (variability due toperformance of different measurements of a same biological unit), morepreferably over both biological and technical variability.

To measure technical variability a Cs:Ts or a Cr:Tr ratio is measuredfrom a given sample multiple times with the method of choice (e.g. atleast 3 or more times, or 5 or more times depending on the variabilityof the methods chosen for measurement as will be understood by a skilledperson) and statistical analysis is performed on the resultingdistribution (e.g. standard error of the mean, or standard distributiondepending on the number of samples used as will be understood by askilled person). Technical variability would depend on the measurementmethod chosen, as different methods have different accuracy, upperquantitative limits and more importantly lower quantitative limits aswill be understood by a skilled person. For example RNA sequencing andreverse transcription digital PCR are methods with low technicalvariability.

To measure biological variability, a Cs:Ts or a Cr:Tr ratio is measuredfrom multiple samples (in particular one can use three resistant andthree susceptible samples, or preferably at least 5 resistant and 5susceptible samples) with a method that has minimal technicalvariability such as RNA sequencing or others identifiable by a skilledperson upon of reading of the present disclosure.

Statistical significance can be defined using a desired percentconfidence. A common choice would be a 95% confidence interval or a 99%confidence interval (for relevant descriptions see Devore 2017 [5].Additional description of statistical analysis used in single-molecule(digital) measurements to resolve differences between two distributionsis provided in Kreutz et al 2011. [6]

In preferred embodiments, to qualify for a marker gene differentiallyexpressed in the treated sample of the susceptible isolate or specimenand in the treated sample of the resistant isolate or specimen, thedifference between the (Cs:Ts) value and resistant (Cr:Tr) value isadjusted to reduce the impact of biological variability and/or technicalvariability, more preferably of both biological and technicalvariability. Accordingly, in some embodiments, the method to identify amarker, further comprises normalizing the susceptible (Cs:Ts) value andthe resistant (Cr:Tr) value prior to selecting a marker genedifferentially expressed in the treated samples.

The wording “normalizing” and “normalization” as used herein refer toadjustments of a value related to a quantified amount to account forvariations. In particular normalization of a value can be performed toaccount for a variation in a parameter associated with the detection ofthe quantified amount, such as variations in an amount of startingmaterial, variations in an amount of sample, variations in bacterialconcentration of sample, variations due to biological variability andvariations due to technical variability.

Normalizing the susceptible (Cs:Ts) value and the resistant (Cr:Tr)value is performed with a reference measurement of RNA, DNA or cellnumber, the number of samples, the volume of sample used, theconcentration of sample used, the effective amount of sample used and/ora related ratio in a control and in a treated sample. Effective amountof sample can be calculated by for example measuring the volumes andconcentration of the sample used. Normalizing the susceptible (Cs:Ts)value can be performed by dividing the control susceptible geneexpression by a reference measurement in the control susceptible sampleand dividing the treated susceptible gene expression by the referencemeasurement in the treated susceptible sample. Normalizing the resistant(Cr:Tr) value can be performed by dividing the control resistant geneexpression by a reference measurement in the control resistant sampleand dividing the treated resistant gene expression by the referencemeasurement in the treated resistant sample. In addition, thenormalization ratio for susceptible sample can be calculated by dividingthe control susceptible reference measurement by the treated susceptiblereference measurement. Normalizing the susceptible (Cs:Ts) value can beperformed by dividing the (Cs:Ts) value by a susceptible normalizationratio. The normalization ratio for resistant sample can be calculated bydividing the control resistant reference measurement by the treatedresistant reference measurement. Normalizing the resistant (Cr:Tr) valuecan be performed by dividing the (Cs:Ts) value by a resistantnormalization ratio.

In some embodiments, normalization can be performed with referencemeasurement of cells such as cell number and/or a related ratio (FIGS.3A-B).

In some embodiments of these embodiments, the reference measurement is ameasurement that reflects the number of target cells. For example, priorto the calculation of a CT ratio, the RNA expression in the untreatedcontrol sample and the RNA expression in the treated sample would bedivided by a cell normalization ratio between number of target cells inthe treated sample and number of target cells in the control samplewhich can be calculated from other measurements such as optical density,turbidity, increase in intensity of a colorimetric, fluorogenic, orluminescent metabolic indicator or a live/dead indicator, colonycounting after plating, amount of pathogen-specific DNA and amount ofpathogen-specific RNA as will be understood by a skilled person,.

In some embodiments, normalization can be performed with referencemeasurement of DNA and/or a related normalization ratio.

In some of these embodiments, the reference measurement is a measurementthat reflects the amount of DNA of the target pathogen. For example, theamount of DNA of the target pathogen present could be measured usingreal time polymerase chain reaction, digital polymerase chain reaction,digital isothermal amplification, real time isothermal amplification,and/or other nucleic acid quantification techniques described herein.One or more DNA target sequences from the genome of the target pathogencan be used for estimating the amount of DNA of the target pathogen.Preferably, DNA sequences conserved within this organism are used.

For example, prior to the calculation of the CT ratio, the RNAexpression in the untreated control sample would be divided by theamount of DNA of the target pathogen measured to be present in thecontrol sample, and the RNA expression in the treated sample would bedivided by the amount of DNA of the target pathogen measured to bepresent in the treated sample. In addition or in the alternative priorto the calculation of the CT ratio, a DNA normalization ratio can beprovided by dividing the amount of DNA of the target pathogen measuredto be present in the control sample and the amount of DNA of the targetpathogen measured to be present in the treated sample. The RNAexpression in the untreated control sample and the RNA expression in thetreated sample can then be divided by the DNA normalization ratio tonormalize the related value.

In some embodiments, normalization can be performed with reference to anRNA measurement and/or a related ratio. In particular, in thoseembodiments, the normalization can be performed using the expressionvalue of a reference RNA, preferably selected among RNA expressed by themicroorganism with low variability among strains of the microorganism.

In some of these embodiments, prior to the calculation of a CT ratio,the RNA expression value of a marker in the treated and/or in theuntreated control sample would be divided by the expression value of thereference RNA in the treated and/or untreated control samplerespectively. In addition or in the alternative, prior to thecalculation of a CT ratio, the RNA expression in the untreated controlsample and the RNA expression in the treated sample can be divided by aRNA normalization ratio provided by the expression value of thereference RNA in the untreated control sample divided by the expressionof the reference RNA in the treated sample. The expression value thereference RNA can be detected by detecting the RNA and/or thecorresponding cDNA in the microorganism.

In some embodiments, also the susceptible (Cs:Ts) value and theresistant (Cr:Tr) value can be normalized with respect to a referenceparameter and/or a related ratio.

For example, normalization of the susceptible (Cs:Ts) value can beperformed by dividing the susceptible (Cs:Ts) value of a targettranscript in an untreated control sample by the expression of a controltranscript such as 16S rRNA and/or 23S rRNA in the untreated controlsample, and by dividing the susceptible (Cs:Ts) value of the targettranscript in the treated sample by the expression of the same controltranscript (e.g. 16S rRNA and/or 23S rRNA) in the treated sample. Inaddition or in the alternative normalizing the susceptible (Cs:Ts) valuecan be performed by dividing the susceptible (Cs:Ts) value by asusceptible control (Csc:Tsc) value of a control transcript (e.g. 16SrRNA or 23S rRNA) wherein the susceptible control (Csc:Tsc) value iscalculated by dividing a gene expression value of the control transcript(e.g. 16S rRNA or 23S rRNA) in the control susceptible sample by a geneexpression value of the control transcript (e.g. 16S rRNA or 23S rRNA)in the treated susceptible sample. In some embodiments, the controltranscript can be ribosomal rRNA such as 16S rRNA or 23S rRNA.

Normalization of the resistant (Cr:Tr) value can be performed bydividing the resistant (Cr:Tr) value of a target transcript in anuntreated control sample by the expression of 16S rRNA and/or 23S rRNAin the untreated control sample, and by dividing the resistant (Cr:Tr)value of the target transcript in the treated sample by the expressionof 16S rRNA and/or 23S rRNA in the treated sample. In addition or in thealternative Normalizing the resistant (Cr:Tr) value can be performed bydividing the resistant (C:T) value by a resistant control (Crc:Trc)value of a control transcript (16S rRNA or 23S rRNA) wherein theresistant control (Crc:Trc) value is calculated by dividing a geneexpression value of the control transcript (16S rRNA or 23S rRNA) in thecontrol resistant sample by a gene expression value of the controltranscript (16S rRNA or 23S rRNA) in the treated resistant sample.

The term “control transcript” refers to a transcript with a fold changein gene expression between control and treated samples (C:T ratio) thatis substantially the same in the resistant and susceptible samples. Insome embodiments, the CT ratio of the control transcript is within a0.1-10 range, preferably within 0.5 to 2.0 range, more preferably within0.75 and 1.25 range.

In preferred embodiments, a control transcript is selected so that thepercentage change from control to treated gene expression is less than25%, more preferably less than 10%. For example, in some embodimentscontrol transcripts are selected so this C:T ratio is close to 1.0 inboth resistant and susceptible samples. Preferably, control transcriptsare selected so this C:T ratio has low technical and biologicalvariability, for example described by standard deviation with value ofless than 0.5, less than 0.4, less than 0.3, less than 0.2, less than0.1. In some embodiments, high-abundance transcripts (for example,transcripts in the top 10% of most expressed transcripts) are used toachieve low technical variability. Preferably, control transcripts areselected so this C:T ratio has low biological variability. Transcriptswith high expression and low biological variability which are notaffected by the antibiotic treatment are good candidates for controltranscripts.

Exemplary RNAs with a log2 fold change less than 0.32 (corresponding to<25% change) that can be used as control transcripts is reported inTable 1 below. The fold change is calculated as the average over the six(three susceptible and three resistant) isolates sequenced. Theexpression guidelines follow the same as in markers.

In Table 1, the GeneID and Gene Name columns are respectively theidentification or reference and name or description of the controltranscript gene from NCBI FA1090. Susc. Fold Change column representsthe average Log2 C:T ratio for the three susceptible isolates sequencedand Susc. Control column represents the average TPM for the threesusceptible isolates sequenced.

TABLE 1 List of exemplary control RNA with low C:T ratios Geneid GeneName DNA, cDNA and RNA sequences Log2 Susc. Fold Change Susc. ControlNGO0066a opacity protein SEQ ID NO: 231-233 in ANNEX E -0.170968576392.786073 NGO0070 opacity protein opA58 SEQ ID NO: 234-236 in ANNEX E-0.209702876 233.9872447 NGO0372 amino acid ABC transportersubstrate-binding protein SEQ ID NO: 237-239 in ANNEX E 0.00367789758.3587698 NGO0374 amino acid ABC transporter ATP-binding protein SEQID NO: 240-242 in ANNEX E -0.201484006 301.4382091 NGO0399 protease HtpXSEQ ID NO: 243-245 in ANNEX E -0.01276968 195.791542 NGO0453 type IVpilus assembly protein PilV SEQ ID NO: 246-248 in ANNEX E -0.304641252102.3440114 NGO0571 hypothetical protein SEQ ID NO: 249-251 in ANNEX E-0.036612448 150.1617177 NGO0632 Fe-S)-cluster assembly protein SEQ IDNO: 252-254 in ANNEX E 0.020285087 284.094415 NGO0633 iron-sulfurcluster assembly scaffold protein SEQ ID NO: 255-257 in ANNEX E0.005118807 205.6161095 NGO0678 hypothetical protein SEQ ID NO: 258-260in ANNEX E -0.289054194 140.5781892 NGO0926 peroxiredoxin familyprotein/glutaredoxin SEQ ID NO: 261-263 in ANNEX E -0.2063135131041.933939 NGO0936 elongation factor P SEQ ID NO: 264-266 in ANNEX E-0.139160962 220.8383642 NGO0950a opacity protein SEQ ID NO: 267-269 inANNEX E -0.023532111 210.4893677 NGO1040a opacity protein SEQ ID NO:270-272 in ANNEX E -0.230149948 366.4512778 NGO1073a opacity protein SEQID NO: 273-275 in ANNEX E -0.071137135 260.6204103 NGO_r02 23S ribosomalRNA SEQ ID NO: 324-326 in ANNEX E 0.05763481 98595.48132 NGO_r03 16Sribosomal RNA SEQ ID NO: 327-329 in ANNEX E 0.075840851 09877.3255NGO1225 peptidyl-prolyl isomerase SEQ ID NO: 276-278 in ANNEX E-0.269241544 210.4315667 NGO1277a opacity protein SEQ ID NO: 279-281 inANNEX E -0.22403411 774.1660932 NGO_r05 23S ribosomal RNA SEQ ID NO:330-332 in ANNEX E 0.056291313 105513.464 NGO_r06 16S ribosomal RNA SEQID NO: 333-335 in ANNEX E 0.075380208 109869.8039 NGO1513 opacityprotein OpaD SEQ ID NO: 282-284 in ANNEX E 0.219592047 467.3481454NGO1553a opacity protein SEQ ID NO: 285-287 in ANNEX E 0.046438846199.2181833 NGO_r08 23S ribosomal RNA SEQ ID NO: 336-338 in ANNEX E0.055364901 113073.5889 NGO_r09 16S ribosomal RNA SEQ ID NO: 339-341 inANNEX E 0.077151808 109869.6765 NGO1762 acpP SEQ ID NO: 288-290 in ANNEXE 0.108200968 418.8600381 NGO1842 Tuf SEQ ID NO: 291-293 in ANNEX E0.124500308 948.5850148 NGO_t45 tRNA-Trp SEQ ID NO: 318-320 in ANNEX E0.08302137 658.3452457 NGO_t47 tRNA-Gly SEQ ID NO: 321-323 in ANNEX E0.292430237 660.2453417 NGO1871 Peptide deformylase SEQ ID NO: 294-296in ANNEX E 0.046048547 201.2627628 NGO_r11 23S ribosomal RNA SEQ ID NO:342-344 in ANNEX E 0.059281774 109175.2904 NGO_r12 16S ribosomal RNA SEQID NO: 10-12 in ANNEX E 0.080622568 109611.2649 NGO1908 pilus retractionprotein PilT SEQ ID NO: 297-299 in ANNEX E -0.17727476 302.6151055NGO1982 hypothetical protein SEQ ID NO: 300-302 in ANNEX E 0.181935722123.4307398 NGO2060a opacity protein SEQ ID NO: 303-305 in ANNEX E0.014421302 237.2782045 NGO2084 membrane protein SEQ ID NO: 306-308 inANNEX E 0.242929753 158.6654013 NGO2134 rpsU SEQ ID NO: 309-311 in ANNEXE 0.171806238 710.1798633 NGO2145 ATP synthase subunit C SEQ ID NO:312-314 in ANNEX E 0.08725091 432.1571821 NGO2146 ATP synthase subunit BSEQ ID NO: 315-317 in ANNEX E 0.188542222 274.7458871

In some embodiments, the control transcript can be a ribosomal RNA,including 23S rRNA, 16S rRNA, 5S rRNA and other RNA component ofribosome.

In some embodiments, 16S rRNA or 23 rRNA are used as control transcriptsfor normalization. Exemplary control transcripts are listed in Table 2:

TABLE 2 List of exemplary 16S ribosomal RNA and 23S ribosomal RNA usedas control transcripts for normalization Locus Tag Gene Description DNA,cDNA and RNA sequences Average Fold Change Average Relative Abundance(TPM) A9Y61_RS06450 or NGO_r02 23S ribosomal RNA SEQ ID NOs: 1 to 3 inANNEX A 1.054 110136.512 A9Y61_RS06465 or NGO_r03 16S ribosomal RNA SEQID NOs:4 to 6 in ANNEX A 1.048 99551.420 A9Y61_RS07175 or NGO_r05 23Sribosomal RNA SEQ ID NOs:7 to 9 in ANNEX A 1.054 110037.026A9Y61_RS07190 or NGO_r04 16S ribosomal RNA SEQ ID NOs:13 to 15 in ANNEXA 1.050 105158.011 A9Y61_RS09315 or NGO_r08 23S ribosomal RNA SEQ IDNOs:16 to 18 in ANNEX A 1.054 110108.563 A9Y61_RS09330 or NGO_r09 16Sribosomal RNA SEQ ID NOs:19 to 21 in ANNEX A 1.048 105226.036 A9Y61_RS10490 Or NGO_r11 23S ribosomal RNA SEQ ID NOs:22 to 24 in ANNEX A 1.054110097.800 A9Y61_RS 10505 or NGO_r12 16S ribosomal RNA SEQ ID NOs:25 to27 in ANNEX A 1.048 05322.731

In some embodiments, control transcript according to the instantdisclosure can have a sequence identity of at least 80%, or 90%, up to100% of the markers listed in Table 1 and 2. In particular markers ofthe instant disclosure can be have sequence identity of 93%, 94%, 95%,96%, 97%, 98%, or 99% of the sequences indicated in Tables 1 and 2.

The Gene IDs listed above as well as their sequences can be retrievedfrom NCBI database (https://www.ncbi.nlm.nih.gov/nuccore/1036099588) aswill be understood by a person skilled in the art.

For example, in some embodiments, a specific region (such as a gene) ofthe DNA can be measured in in the control and treated sample and used asnormalization DNA measurement, as will be understood by a skilledperson. In some embodiments DNA normalization methods can be performedby PCR or dPCR. In some embodiments, a fluorescence dye thatquantitatively stains DNA can be used as a normalization method.Additional methods to perform normalization DNA measurements areidentifiable by a skilled person upon reading of the present disclosure.

In some embodiments, quantitatively detecting Cs Ts and Cr and Tr can beperformed on a treated sample and corresponding control sample underseveral sets of conditions (e.g. varying treatment times, differentexperimental settings and/or using a plurality of isolates or specimenand/or a plurality of related control and/or treated sample) to providea gene expression pattern for the candidate marker gene formed by thegene expression values detected in each treated and correspondingcontrol samples under each set of conditions. In those embodiments, thedifferential expression of the candidate gene marker is detected withrespect to the corresponding gene expression pattern according toapproaches identifiable by a skilled person upon reading of the presentdisclosure.

In some embodiments, the candidate gene marker is a plurality ofcandidate gene markers. In those embodiments the quantitative detectionof the related expression can be performed by detecting global geneexpression, or patterns of gene expression, in the samples of thesusceptible and resistant isolate or specimen.

The wording “global gene expression” as used herein indicates anexpression level of a population of RNA molecules in cells and tissues.In particular, global gene expression can be performed to detect atranscriptome which is the set of all RNA molecules in one cell or apopulation of cells. Global gene expression is an approach typicallyused to investigate a transcriptional behavior of a biological system inconnection with various biological phenomenon, as global genesexpression can provide quantitative information about the population ofRNA species in cells and tissues. The wording “Pattern of geneexpression” refers to gene expression of multiple markers, or geneexpression of the same marker over multiple conditions.

In embodiments herein described detecting global gene expression andpattern of gene expression can be performed using DNA microarrays,Nanostring, RNA-Seq, digital PCR, bulk qPCR, isothermal techniques suchas LAMP or digital isothermal amplification techniques, and othernucleic acid quantification techniques described herein to measure thelevels of RNA species in biological systems.

In those embodiments, providing a susceptible (Cs:Ts) value for thecandidate marker gene in the susceptible isolate or specimen andproviding a resistant (Cr:Tr) value for the candidate marker gene in theresistant isolate or specimen can be performed by

-   quantitatively detecting a control susceptible gene expression value    Cs for each of the plurality of genes in the control susceptible    sample and a control resistant gene expression value Cr for each of    a plurality of genes in the control resistant sample,-   quantitatively detecting a treated susceptible gene expression Ts    value for each of the plurality of genes in the treated susceptible    sample and a treated resistant gene expression value Tr for each of    a plurality of genes in the treated resistant sample,-   providing a susceptible (Cs:Ts) value and a corresponding resistant    (Cr:Tr) value for each of the plurality of genes.

In those embodiments, the method further comprises selecting a set ofmaker genes differentially expressed in the treated sample of thesusceptible isolate or specimen and in the treated sample of theresistant isolate or specimen by identifying the genes with thesusceptible (Cs:Ts) value different from the corresponding resistant(Cs:Ts) value.

In some embodiments, to qualify for a marker gene differentiallyexpressed in the treated sample of the susceptible isolate or specimenand in the treated sample of the resistant isolate or specimen, thedifference between the susceptible (C:T) value and resistant (C:T) valueis larger than a threshold.

In some embodiments, the method further comprises selecting thecandidate gene markers having a Cs:Ts and/or Cr:Tr above or below athreshold of significance respectively. In some embodiments, anindividual threshold is established for each of the plurality of markersin accordance with approaches of the present disclosure. In particularthe threshold can be based on the knowledge of a distribution of aparameter indicative of the expression of one or more transcripts, toinclude transcripts differentially expressed in treated vs controlsample across the distribution. For example to establish the thresholdfor each marker, C:T measurements are performed on a plurality ofresistant and susceptible isolates, optionally including isolates withintermediate resistance. Threshold values can then be chosen tomaximally separate C:T ratios for resistant and susceptible isolates. Ifa plurality of markers is used to determine antibiotic susceptibility ofan organism, a number of algorithms can be used to interpret suchinformation to make the determination. For example, weighted average orweighted sum of C:T ratios of the markers can be compared to theweighted average or weighted sum of the thresholds. Machine learning andpattern-recognition algorithms can be used. Measured fold-changes can bemultiplied and compared to multiplied thresholds for multiple markers.

In detections when there is overlap between C:T ratios of resistant andsusceptible isolates, various classification models can be used to mapthe C:T ratios between the susceptible and resistant groups. Forexample, receiver operating characteristic (ROC curve) can be analyzedand used to set optimal threshold. (seehttps://en.wikipedia.org/wiki/Receiver_operating_characteristic at thefiling date of the present disclosure). ROC curve can be used to selectoptimal balance of analytical specificity and sensitivity of the test.In particular, the wording “analytical sensitivity” indicates themethod’s ability to detect the target molecule at low levels in asample. This is defined as the lowest concentration of RNA in a samplethat can be detected >95% of the time. The wording “analyticalspecificity” indicates the method’s ability to detect the intendedtarget in a complex sample. This refers to the ability of the method todifferentiate between the intended target and similar targets from otherbacterial species and the ability of the method to overcome inhibitorsfrom the sample. When tested with clinical samples, ROC curve can beused to select optimal balance of clinical specificity and sensitivityof the test. Furthermore, prevalence data can be incorporated to providea further refinement or predicted specificity and sensitivity of thetest.

Additionally, in those embodiments detection where there is overlapbetween C:T ratios of resistant and susceptible isolates, the thresholdcan be also set in view of the severity of one type of error versusanother, to reduce or minimize major errors even if this requires anincrease of minor errors. For example, in case of overlaps between C:Tratios of resistant and susceptible isolates the threshold can be set toreduce up to minimize false susceptible (considered a more problematicerror in terms of resulting treatment) increasing the expectedpercentage of false resistant. In some embodiments, the method can beperformed with a plurality of susceptible and/or resistant isolateshaving genetic variability.

The wording “genetic variability” refers to either the presence of, orthe generation of, genetic differences in a microorganism. The term“genetic variability” is defined as the formation of individualsdiffering in genotype, or the presence of genotypically differentindividuals. Therefore, Genetic variability refers to the difference ingenotype between specific organisms while biological variability refersto the phenotypic differences between specific organisms, in this caseRNA response to an antibiotic given for a specified amount of time.

Accordingly, a genetic variant indicates a genetic difference from areference genome. The genetic variant can be used to describe analteration (such as insertions, deletions, and /or replacement ofnucleotides) that can be a result of mutations, recombination as will beunderstood by a person skilled in the art. Exemplary genetic variantscomprise single base-pair substitution, also known as single nucleotidepolymorphism (SNP), insertion or deletion of a single stretch of DNAsequence that can range for example from two to hundreds of base-pairsin length, and structural variation including copy number variation andchromosomal rearrangement events. The structural variation typicallyinclude deletion, insertion, inversion, duplication and copy numbervariation of the individual nucleic acids as will be understood by aperson skilled in the art.

In particular in some embodiments, the susceptible and resistantisolates or specimen used herein for identifying a marker of antibioticsusceptibility comprise at least three different susceptible isolates orspecimen and at least three different resistant isolates or specimen,preferably at least five different susceptible isolates or specimen andat least five different resistant isolates.

In preferred embodiments, the susceptible and resistant isolates orspecimen used herein for identifying a marker of antibioticsusceptibility are selected to differ in genotypes and in biologicalresponses to antibiotic administration to maximize genetic andbiological variability of the isolates or specimen used for identifyinga marker.

In some embodiments, selection of susceptible and resistant isolates orspecimen used for identifying a marker of antibiotic susceptibility toincrease or maximize genetic variability can be performed by sequencingthe genomes of multiple isolates and selecting genetically differentisolates or by obtaining isolates from different clusters from anisolate depository such as the CDC isolate bank or others entitiesidentifiable by a skilled person. Hierarchical clustering based ongenetic distance can be performed by first generating a SNP profile foreach isolate against a reference genome (NCBI FA1090). Then amaximum-likelihood based inference method for phylogenetic treegeneration can be performed to cluster isolates by genetic variabilityusing tools such as RAxML or Garli and additional tools identifiable bya skilled person. Isolates can then be chosen from a plurality ofclusters after hierarchical phylogenetic clustering.

In some embodiments, selection of susceptible and resistant isolates orspecimen used for identifying a marker of antibiotic susceptibility toincrease or maximize biological variability in RNA expression can beperformed on a full transcriptome scale, (e.g. by detecting thetransctiptome through RNA sequencing or on a gene specific scale (e.g.by detecting the specific gene expression through PCR based methods)following administration of an antibiotic and then calculating therelated C:T ratio. Reference is made in this connection to the resistantisolates in FIG. 5 of the instant disclosure wherein detection of thespread of the porB C:T ratios would provide an estimate for thebiological variability for porB. Additional indicator of biologicalvariability comprise resistance profile to antibiotics indicated forexample in terms of MIC for one or more antbiotics.

In preferred embodiments, selection of susceptible and resistantisolates or specimen used herein for identifying a marker of antibioticsusceptibility to select isolates having a high prevalence in a targetregion (area where the marker is intended to be used, such a city acounty, a state, a country or larger regions formed by groups ofcountries or the entire world) based on surveys or other epidemiologicaldata on the strains of a certain microorganism in the target region. Inparticular, one or more isolates can be selected that cluster togetherwith strains accounting for at least 75% more preferably at least 85%even more preferably at least 90% or most preferably at least 95% of thestrains infecting individuals in the target region.

In preferred embodiments selection of susceptible and resistant isolatesor specimen used for identifying a marker of antibiotic susceptibilityis performed by selecting at least 3 to 5 isolates maximizing geneticvariability, biological variability while selecting the isolates with aprevalence of at least 75% more preferably at least 85% even morepreferably at least 90% or most preferably at least 95% of the strainsinfecting individuals in a target region.

Following selection of a plurality of isolates preferably maximizinggenetic and biological variability and prevalence in a target region,candidate markers can be tested with methods herein described.

In some embodiments, detecting expression of a candidate gene marker ina plurality of the selected susceptible isolates and in a plurality ofthe selected resistant isolates (at least three preferably at least 5)gene expression upon antibiotic exposure is performed by detectingexpression a plurality of candidate gene markers (e.g. at least 2, atleast 5, at least 10, at least 50 or, at least 100 or 300 or moredepending on the genome size and the candidate markers selected and thedetection technique selected). In those embodiments, detectingexpression a plurality of candidate gene markers can be performed bydetecting patterns of gene expression and/or global gene expression uponantibiotic exposure in a control sample and in a treated sample of eachof the plurality of the selected susceptible isolates and in each of theplurality of the selected resistance isolates.

In some embodiments wherein quantitatively detecting expression of acandidate marker genes is performed by quantitatively detecting aplurality of candidate marker gene, and/or by quantitatively detectingexpression of a candidate marker gene in a plurality of resistant and/orsusceptible isolate, the method to identify a marker of antibioticsusceptibility in a microorganism of the instant disclosure can furthercomprises selecting the candidate gene marker with a transcript having ahigh fold change in expression upon antibiotic exposure.

A high fold change is defined as at least two folder change or higher.In particular, in some embodiments, a significant shift of fold change(larger than 4) in transcript levels can be observed within 5 min ofantibiotic exposure. In some typically more infrequent instances genescan respond to antibiotic exposure with changes as large as 6-foldwithin 5 min.

The term “transcript” as used herein refers to any ribonucleic acidsequence provided in the microorganism without limitation to anyspecific type, function or length. Transcripts include messenger RNA(mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA) of any length.

In some embodiments, the method to identify an RNA marker of antibioticsusceptibility further comprises validating the candidate markers bydetermining whether the candidate markers respond consistently across alarge pool of isolates with genetic variability.

The validation of candidate markers can be performed by selecting thecandidate markers with the highest abundance and fold change and usingthese selected candidate markers to determine the susceptibility ofclinical isolates with known susceptibility/resistance. The clinicalisolates can be obtained from the Centers for Disease Control (CDC)Antimicrobial Resistance Isolate Bank (see Example 10) and preferablyrepresent a large degree of genetic variation or difference.

Validate markers are identified as markers showing consistency in theirability to correctly determine susceptibility or resistant of theclinical isolates.

In some embodiments, wherein quantitatively detecting expression of acandidate marker genes is performed by quantitatively detecting aplurality of candidate marker gene, and/or by quantitatively detectingexpression of a candidate marker gene in a plurality of resistant and/orsusceptible isolates or specimen, the method to identify a marker ofantibiotic susceptibility in a microorganism of the instant disclosurefurther comprises selecting a candidate gene marker having transcriptsrepresentative of different biochemical pathways.

The term “biochemical pathways” refer to a sequence of chemical orbiochemical reactions catalyzed by enzymes in which a product of oneenzyme acts as the substrate for the next.

In some embodiments of the method to identify a marker of antibioticsusceptibility in a microorganism of the instant disclosure, themicroorganism is a slow growing microorganism, a microorganism with atranscriptome which is not characterized and/or a microorganism thatlacks a transcriptional SOS response to DNA damage.

The term “slow growing” as used herein indicates an organism with adoubling time longer than 30 minutes.

In some embodiments of the method to identify a marker of antibioticsusceptibility in a microorganism of the instant disclosure, theantibiotic is a fluoroquinolone. The term “fluoroquinolone” as usedherein indicates a group of antibiotics containing a fluorine atom intheir chemical structure. Fluoroquinolones are usually effective againstboth Gram-negative and Gram-positive bacteria. Exemplary fluoroquinoloneinclude levofloxacin, ofloxacin, gatifloxacin, moxifloxacin, andnorfloxacin.

In some of these embodiments, the antibiotic for treating the sampleherein described, the concentration of the antibiotic can be provided ata concentration between 0.015 microgram/mL and 16.0 microgram/mL.

In some of these embodiments, the fluoroquinolones is ciprofloxacin. Insome of these embodiments, the concentration of antibiotic used duringexposure or treatment can be any concentration between the susceptibleand resistant MIC breakpoints of the target organism. For example, forexposure or treatment of Ng with ciprofloxacin, the concentration ofantibiotic used could any concentration ≥ 0.06 microgram/mL (thesusceptible MIC breakpoint for ciprofloxacin for Neisseria gonorrhoeae)and ≤ 1.00 microgram/mL (the resistant MIC breakpoint for ciprofloxacinfor Neisseria gonorrhoeae). In some embodiments, for example when fasterresponses are desired, higher than breakpoint concentrations can beused.

In some embodiments of the method to identify a marker of antibioticsusceptibility in a microorganism of the instant disclosure theantibiotic is an antibiotic inhibiting the enzymes topoisomerase II (DNAgyrase) and topoisomerase IV, thereby inhibiting cell division. Examplesinclude Aminocoumarin antibiotics such as Novobiocin, AlbamycinCoumermycin, Clorobiocin, and their derivatives, Simocyclinones andderivatives, moxifloxacin, ciprofloxacin, azithromycin, tetracycline,and ceftriaxone.

Additional examples of such antibiotics comprise novel bacterialtopoisomerase inhibitors (NBTIs) and in particular Type I NBTIs such asgepotidacin and its analogues, GSK945237, AM-8722, 1,5-naphthyridineoxabicyclooctane linked NBTIs, and type II NBTIs, such as quinolonepyrimidone trione-1 (QPT-1) Zoliflodacin (AZD0914), isothiazoloneanalogue REDX04957 and its two enantiomer forms, REDX05967 andREDX05990,. Further examples comprise nalidixic acid, oxolinic acid,norfloxacin, iprofloxacin, levofloxacin, moxifloxacin, Gemifloxacin,EDX04139, REDX05604, REDX05931, kibdelomycin thiosemicarbazide;4,5-dibromo-N-(thiazol-2-yl)-1H-pyrrole-2-carboxamide, cyclothialidine;pyrazolopyridone, 4-(4-(3,4-dichloro-5-methyl-1H-pyrrole-2carboxamido),piperidin-1-yl)-4-oxobutanoic acid,trans-4-(4,5-dibromo1H-pyrrole-2-carboxamide)cyclohexyl)glycine,pyrazolopyridones, cyclothialidines and their analogues, GR122222X,cinodine, albicidin, clerocidin, microcin B17, CcdB, an pentapeptiderepeat proteins Qnr and MfpA, as well as additional antibioticsidentifiable by a skilled person (see e.g. Badshah and Ullah 2018 [7]and Collin et al. 2018 [8]).

In the instant disclosure, an RNA marker of antibiotic susceptibility ina microorganism is described, as well as a corresponding marker geneand/or a corresponding cDNA are described, which can be obtained by themethod to identify an RNA marker of antibiotic susceptibility

In some embodiments, the RNA markers comprise RNA markers encoding aribosomal protein. The term “ribosomal protein” is the protein componentof ribosome that in conjunction with rRNA make up the ribosomal subunitsinvolved in the cellular process of translation. Prokaryotic bacteriaand archaea have a 30S small subunit and a 50S large subunit.Accordingly, some of these mRNA markers disclosed herein comprise mRNAmarkers encoding 50S ribosomal proteins and mRNA markers encoding 30Sribosomal proteins.

Exemplary mRNA markers encoding ribosomal proteins include mRNA encoding50S L4, 50S L13, 30S S12, 50S L27, 50S L19, 30S S19, 50S L2, 50S L22,50S L32, 30S S1, 50S L21, 50S L33, 30S S16, 50S L28.

An additional list of exemplary mRNA markers of N. gonorrhoeae encodingribosomal proteins is also shown in Table 5 of the instant applicationincluding rplD, rplM, rpsL, rpmA, rplS, rpsS, rplB, rplV, rpmF, rpsA,rplU, rpmG, rpsP, and rpmB.In some embodiments of the method hereindescribed to identify a marker of antibiotic susceptibility in amicroorganism of the instant disclosure, the microorganism is N.gonorrhoeae.

Neisseria gonorrhoeae is one type of proteobacteria that causes thesexually transmitted genitourinary infection gonorrhea as well as otherforms of gonococcal disease including disseminated gonococcemia, septicarthritis, and gonococcal ophthalmia neonatorum. The term “Neisseriagonorrhea” includes all strains of N. gonorrhoeae identifiable by aperson skilled in the art. Neisseria gonorrhea also includes geneticvariants of different strains. One may determine whether the targetorganism is N. gonorrhoeae by a number of accepted methods, includingsequencing of the 16S ribosomal RNA (rRNA) gene, as described inChakravorty et al (2007) for N. gonorrhoeae. [9]

In some embodiments of the method herein described to identify a markerof antibiotic susceptibility in a microorganism of the instantdisclosure, the microorganism is Neisseria meningitidis. Neisseriameningitidis, often referred to as meningococcus, is a Gram-negativebacterium that can cause meningitis and other forms of meningococcaldisease such as meningococcemia, a life-threatening sepsis.

In some embodiments of the method herein described to identify a markerof antibiotic susceptibility in a microorganism of the instantdisclosure, the RNA marker is not a direct target of the antibiotic. Forexample in some embodiments where the antibiotic is a quinolone and inparticular ciprofloxacin, the selected markers are not identified targetof gyrA, parC and/or recA identified as target for ciprofloxacin.

In some embodiments of the instant disclosure wherein the microorganismis N. gonorrhoeae, the markers can be selected from: a transcript of N.gonorrhoeae gene having locus tag NGO0340, a transcript of N.gonorrhoeae gene having locus tag NGO1837, a transcript of N.gonorrhoeae gene having locus tag NGO1843, a transcript of N.gonorrhoeae gene having locus tag having locus tag NGO2024, a transcriptof N. gonorrhoeae gene having locus tag NGO1845, a transcript of N.gonorrhoeae gene having locus tag NGO1677, a transcript of N.gonorrhoeae gene having locus tag NGO1844, a transcript of N.gonorrhoeae gene having locus tag NGO0171, a transcript of N.gonorrhoeae gene having locus tag NGO1834, a transcript of N.gonorrhoeae gene having locus tag NGO0172, a transcript of N.gonorrhoeae gene having locus tag NGO1835, a transcript of N.gonorrhoeae gene having locus tag NGO1673, a transcript of N.gonorrhoeae gene having locus tag NGO1833, a transcript of N.gonorrhoeae gene having locus tag NGO2173, a transcript of N.gonorrhoeae gene having locus tag NGO0604, a transcript of N.gonorrhoeae gene having locus tag NGO0016, a transcript of N.gonorrhoeae gene having locus tag NGO1676, a transcript of N.gonorrhoeae gene having locus tag NGO1679, a transcript of N.gonorrhoeae gene having locus tag NGO1658, a transcript of N.gonorrhoeae gene having locus tag NGO1440, a transcript of N.gonorrhoeae gene having locus tag NGO0174, a transcript of N.gonorrhoeae gene having locus tag NGO0173, a transcript of N.gonorrhoeae gene having locus tag NGO0592, a transcript of N.gonorrhoeae gene having locus tag NGO1680, a transcript of N.gonorrhoeae gene having locus tag NGO0620, a transcript of N.gonorrhoeae gene having locus tag NGO1659, a transcript of N.gonorrhoeae gene having locus tag NGO1291, a transcript of N.gonorrhoeae gene having locus tag NGO0648, a transcript of N.gonorrhoeae gene having locus tag NGO0593, a transcript of N.gonorrhoeae gene having locus tag NGO1804, a transcript of N.gonorrhoeae gene having locus tag NGO0618, a transcript of N.gonorrhoeae gene having locus tag NGO0619, a transcript of N.gonorrhoeae gene having locus tag NGO1812, a transcript of N.gonorrhoeae gene having locus tag NGO1890, a transcript of N.gonorrhoeae gene having locus tag NGO2098, a transcript of N.gonorrhoeae gene having locus tag NGO2100, a transcript tRNA havingGeneID A9Y61_RS02445 or NGO_t12, a transcript tRNA having GeneIDA9Y61_RS04515 or NGO_t15, a transcript tRNA having GeneID A9Y61_RS04510or NGO_t14, a transcript tRNA having GeneID A9Y61_RS09170 or NGO_t37,and a transcript tRNA having GeneID A9Y61_RS00075 or NGO_t01. Thesequences of these transcripts can be retrieved from the publicdatabases in compliance with the International Nucleotide SequenceDatabase Collaboration at the date of filing of the present disclosureas will be understood by a person skilled in the art. In particular, thesequences of these transcript can be identified by entering the locustag or the GenID, alone or in combination with additional informationprovided in the present disclosure, in databases such as National Centerfor Biotechnology Information (NCBI) the European BioinformaticsInstitute (EMBL-EBI) and DNA Data Bank of Japan (DDBJ) at the date offiling of the present disclosure.

In some embodiments the cDNAs of N. gonorrhoeae can have a sequence thatcan be shorter or longer than the sequences in the databases as will beunderstood by a skilled person. In particular, the transcript caninclude a re be up to 30 bp, 40 bp, 50 bp, 60 bp, 70 bp, 80 bp, 90 bp,100 bp, 150 bp, 200 bp, 250 bp, 300 bp, 400 bp, 500 bp, 750 bp, 1000 bp,1500 bp, 2000 bp, 2500 bp , or up to 3000 bp, shorter or longer of thesequence in the database as will be understood by a skilled person.Exemplary sequences for the above markers are provided in Table 3 below.

TABLE 3 List of exemplary marker genes differentially expressed betweenan untreated sample and a sample treated with antibiotics. Locus TagDNA, cDNA and RNA Sequences NGO0340 SEQ ID Nos. 49 to 51 in ANNEX BNGO1837 SEQ ID Nos. 109 to 111 in ANNEX B NGO1843 SEQ ID Nos. 112 to 114in ANNEX B NGO2024 SEQ ID Nos. 124 to 126 in ANNEX B NGO1845 SEQ ID Nos.118 to 120 in ANNEX B NGO1677 SEQ ID Nos. 91 to 93 in ANNEX B NGO1844SEQ ID Nos. 115 to 117 in ANNEX B NGO0171 SEQ ID Nos. 37 to 39 in ANNEXB NGO1834 SEQ ID Nos. 103 to 105 in ANNEX B NGO0172 SEQ ID Nos. 40 to 42in ANNEX B NGO1835 SEQ ID Nos. 106 to 108 in ANNEX B NGO1673 SEQ ID Nos.85 to 87 in ANNEX B NGO1833 SEQ ID Nos. 100 to 102 in ANNEX B NGO2173SEQ ID Nos. 136 to 138 in ANNEX B NGO0604 SEQ ID Nos. 58 to 60 in ANNEXB NGO0016 SEQ ID Nos. 34 to 36 in ANNEX B NGO2174 SEQ ID Nos. 139 to 141in ANNEX B NGO2164 SEQ ID Nos. 133 to 135 in ANNEX B NGO1676 SEQ ID Nos.88 to 90 in ANNEX B NGO1679 SEQ ID Nos. 94 to 96 in ANNEX B NGO1658 SEQID Nos. 79 to 81 in ANNEX B NGO1440 SEQ ID Nos. 76 to 78 in ANNEX BNGO0174 SEQ ID Nos. 46 to 48 in ANNEX B NGO0173 SEQ ID Nos. 43 to 45 inANNEX B NGO0592 SEQ ID Nos. 52 to 54 in ANNEX B NGO1680 SEQ ID Nos. 31to 33 in ANNEX B NGO0620 SEQ ID Nos. 67 to 69 in ANNEX B NGO1659 SEQ IDNos. 82 to 84 in ANNEX B NGO1291 SEQ ID Nos. 73 to 75 in ANNEX B NGO0648SEQ ID Nos. 70 to 72 in ANNEX B NGO0593 SEQ ID Nos. 55 to 57 in ANNEX BNGO1804 SEQ ID Nos. 97 to 99 in ANNEX B NGO0618 SEQ ID Nos. 61 to 63 inANNEX B NGO0619 SEQ ID Nos. 64 to 66 in ANNEX B NGO1812 SEQ ID NOs.28 to30 in ANNEX B NGO1890 SEQ ID Nos. 121 to 123 in ANNEX B NGO2098 SEQ IDNos. 127 to 129 in ANNEX B NGO2100 SEQ ID Nos. 130 to 132 in ANNEX BA9Y61_RS02445 or NGO_t12 SEQ ID Nos. 145 to 147 in ANNEX B A9Y61_RS04515or NGO_t15 SEQ ID Nos. 151 to 153 in ANNEX B A9Y61_RS04510 or NGO_t14SEQ ID Nos. 148 to 150 in ANNEX B A9Y61_RS09170 or NGO_t37 SEQ ID Nos.228 to 230 in ANNEX B A9Y61_RS00075 or NGO_t01 SEQ ID Nos. 142 to 144 inANNEX B

In some embodiments, markers according to the instant disclosure canhave a sequence identity of at least 80%, or 90%, up to 100% of themarkers listed in Table 3. In particular, markers of the instantdisclosure can have sequence identity of 93%, 94%, 95%, 96%, 97%, 98%,or 99% of the sequences indicated in Table 3.

The term “sequence identity” refers to a quantitative measurement of theidentity between sequences of a polypeptide or a polynucleotide and, inparticular, indicates the amount of characters that match between twodifferent sequences. Commonly used similarity searching programs, suchas BLAST, PSI-BLAST [10] [11] [12] [13], SSEARCH [14] [15] FASTA[16] andthe HMMER3 9 [17] can produce accurate statistical estimates, ensuringthat protein sequences that share significant similarity also havesimilar structures.

The identity between sequences is typically measured by a process thatcomprises the steps of aligning the two polypeptide or polynucleotidesequences to form aligned sequences, then detecting the number ofmatched characters, i.e. characters identical between the two alignedsequences, and calculating the total number of matched charactersdivided by the total number of aligned characters in each polypeptide orpolynucleotide sequence, including gaps. The identity result isexpressed as a percentage of identity.

Biomarker’s features of the RNA markers of Table 3, such as resistant CTratios and values, susceptible CT ratio values, abundance and thresholdvalues, are further illustrated in

TABLE 4 List of exemplary marker genes differentially expressed betweenan untreated sample and a sample treated with antibiotics Table 4: Listof exemplary marker genes differentially expressed between an untreatedsample and a sample treated with antibiotics Locus Tag AverageSusceptible C:T ratio after 15 min exposure Standard Deviation ofSusceptible C:T ratio after 15 min exposure Average Susceptible RelativeAbundance (TPM) Average Resistant C:T ratio after 15 min exposureStandard Deviation of Resistant C:T ratio after 15 min exposure NGO03402.757 0.811 886.752 0.917 0.014 NGO1837 2.08 0.382 542.158 0.906 0.058NGO1843 2.053 0.089 596.162 0.856 0.036 NGO2024 1.93 0.138 504.857 0.9210.023 NGO1845 1.981 0.083 793.435 0.872 0.03 NGO1677 2.991 0.631 500.9190.983 0.02 NGO1844 2.084 0.051 719.419 0.815 0.034 NGO0171 2.425 0.448425.961 0.913 0.02 NGO1834 2.161 0.123 418.234 0.892 0.055 NGO0172 3.5511.865 273.08 0.892 0.031 NGO1835 2.149 0.21 507.389 0.891 0.061 NGO16734.902 2.876 227.951 0.931 0.005 NGO1833 2.2 0.146 441.363 0.879 0.074NGO2173 2.629 0.25 429.176 0.962 0.019 NGO0604 2.452 0.162 504.385 0.8810.037 NGO0016 3.348 0.64 161.719 0.946 0.034 NGO2174 2.8 0.264 422.6880.955 0.046 NGO2164 2.978 0.257 136.319 0.901 0.043 NGO1676 3.237 0.371626.49 0.936 0.006 NGO1679 5.201 2.268 317.278 0.952 0.074 NGO1658 3.4280.841 127.162 0.957 0.058 NGO1440 4.781 1.963 140.148 0.916 0.065NGO0174 4.187 1.653 368.372 0.885 0.04 NGO0173 4.834 2.216 400.084 0.9670.054 NGO0592 6.014 1.977 145.561 0.92 0.062 NGO1680 5.579 1.488 522.3930.931 0.052 NGO0620 4.147 0.727 61.968 0.937 0.091 NGO1659 5.667 1.79173.859 0.939 0.076 NGO1291 5.105 1.164 77.971 0.998 0.061 NGO0648 4.9591.74 57.357 1.115 0.169 NGO0593 4.643 0.768 70.256 0.951 0.018 NGO18045.062 1.772 103.085 0.901 0.09 NGO0618 4.323 0.445 91.615 0.874 0.063NGO0619 5.758 1.431 63.787 0.88 0.054 NGO1812 4.875 0.352 1142.564 0.8970.027 NGO1890 9.946 6.62 55.955 0.829 0.022 NGO2098 7.087 2.034 30.660.927 0.072 NGO2100 6.593 0.696 24.365 0.816 0.029 A9Y61_RS02445 orNGO_t12 6.495 2.273 72.289 1.113 0.114 A9Y61_RS04515 or NGO_t15 3.0640.94 561.339 1.343 0.109 A9Y61_RS04510 or NGO_t14 2.9 0.827 889.5921.119 0.048 A9Y61_RS09170 or NGO_t37 3.396 1.001 128.355 1.006 0.165A9Y61_RS00075 or NGO_t01 4.086 0.448 64.178 1.046 0.248

In the illustration of Table 4, for each marker, the range of possiblethreshold C:T ratios is calculated as a range between the mean Cr:Trratios for resistant and the mean Cs:Ts ratios for susceptible isolates,and narrowed down further to account for variability of the Cr:Tr ratiosfor resistant and the Cs:Ts ratios of susceptible isolates.

In some embodiments, after the marker is selected, when testing a samplewith bacteria of unknown susceptibility to an antibiotic, the C:T ratiofor this marker obtained from this sample is compared with Cs:Ts andCr:Tr ratios. In some embodiments the C:T ratio thus obtained can beassigned as belonging to susceptible or resistant organism based on athreshold value.

For example, for a marker downregulated in the susceptible bacteria, theCr:Tr values will be smaller than Cs:Ts values and a threshold value canbe set above Cr:Tr value(s) and below Cs:Ts value(s). If a detected C:Tis below threshold, we call it resistant and if CT is above threshold wecall susceptible. In particular the threshold value can be set based onthe knowledge of a distribution of a parameter indicative of theexpression of one or more transcripts, to include transcriptsdifferentially expressed in treated vs control sample across thedistribution. In particular the threshold value for a C:T ratio can beset based on the knowledge of Cs:Ts and Cr:Tr distributions of a giventranscript. In some embodiments, the threshold value is set at theaverage between the means of Cs:Ts and Cr:Tr distributions. In someembodiments, especially when the Cs:Ts and Cr:Tr distributions haveunequal variance, the threshold value is set to between the means ofCs:Ts and Cr:Tr distributions at a value where the overlap between Cs:Tsand Cr:Tr distributions is zero or minimized.

In some embodiments, the threshold value can be selected among any oneof the value within the following ranges 0.931-1.946, 0.964-1.698,0.892-1.964., 0.944-1.792, 0.902-1.898, 1.003-2.360, 0.849-2.033,0.933-1.977, 0.947-2.038., 0.923-1.686, 0.952-1.939, 0.936-2.026,0.953-2.054, 0.981-2.379, 0.918-2.290, 0.980-2.708, 1.001-2.536,0.944-2.721, 0.942-2.866, 1.026-2.933, 1.015-2.587, 0.981-2.818,0.925-2.534, 1.021-2.618, 0.982-4.037, 0.983-4.091, 1.028-3.420,1.015-3.876, 1.059-3.941, 1.284-3.219, 0.969-3.875, 0.991-3.290,0.937-3.878, 0.934-4.327, 0.924-4.523, 0.851-3.326, 0.999-5.053,0.845-5.897, 1.227-4.222 as will be understood by a skilled person uponreading of the present disclosure.

In some embodiments the RNA markers of N. gonorrhoeae herein describedcan have the following sequences indicated properties indicated in Table5.

TABLE 5 List of exemplary marker genes differentially expressed betweenan untreated sample and a sample treated with antibiotics. Locus TagDescription of putative or verified functionality associated to themarker NGO0340 cysteine synthase A (cysK) NGO1837 50S ribosomal proteinL4 (rplD) NGO1843 elongation factor G (fusA) NGO2024 50S ribosomalprotein L13 (rplM) NGO1845 30S ribosomal protein S12 (rpsL) NGO1677 50Sribosomal protein L27 (rpmA) NGO1844 30S ribosomal protein S7 NGO017150S ribosomal protein L19 (rplS) NGO1834 30S ribosomal protein S19(rpsS) NGO0172 tRNA (guanine-N(1)-)-methyltransferase (trmD) NGO1835 50Sribosomal protein L2 (rplB) NGO1673 type IV pilus assembly protein(pilB) NGO1833 50S ribosomal protein L22 (rplV) NGO2173 50S ribosomalprotein L32 (rpmF) NGO0604 30S ribosomal protein S1 (rpsA) NGO0016preprotein translocase subunit (secG) NGO2174 hypothetical proteinNGO2164 GMP synthase (guaA) NGO1676 50S ribosomal protein L21 (rplU)NGO1679 50S ribosomal protein L33 (rpmG) NGO1658 hypothetical proteinNGO1440 macrolide transport protein MacA NGO0174 30S ribosomal proteinS16 (rpsP) NGO0173 ribosome maturation factor RimM (rimM) NGO0592trigger factor (tig) NGO1680 50S ribosomal protein L28 (rpmB) NGO0620aspartate alpha-decarboxylase NGO1659 intracellular septation protein ANGO1291 transcriptional regulator (yebC) NGO0648 membrane proteinNGO0593 ATP-dependent Clp protease proteolytic subunit (clpP) NGO1804(3R)-hydroxymyristoyl-ACP dehydratase (fabZ) NGO0618 membrane proteinNGO0619 2-dehydro-3-deoxyphosphooctonate aldolase NGO1812 major outermembrane protein (porB) NGO1890 glutamate permease; sodium/glutamatesymport carrier protein NGO2098 diaminopimelate decarboxylase NGO2100frataxin-like protein (cyaY) A9Y61_RS02445 or NGO_t12 tRNA-SerineA9Y61_RS04515 or NGO_t15 tRNA-Serine A9Y61 _RS04510 or NGO_t14tRNA-Leucine A9Y61_RS09170 or NGO_t37 tRNA-Arginine A9Y61 _RS00075 orNGO_t01 tRNA-Leucine

In preferred embodiments, the transcript can comprise at least one of atranscript of N. gonorrhoeae gene having locus tag NGO1812, a transcriptof N. gonorrhoeae gene having locus tag NGO1680), a transcript of N.gonorrhoeae gene having locus tag NGO1291, a transcript of N.gonorrhoeae gene having locus tag NGO1673, a transcript of a transcriptof N. gonorrhoeae gene having locus tag NGO0592 and a transcript of N.gonorrhoeae gene having locus tag NGO0340.

In more preferred embodiments, the transcript comprises or is at leastone of a transcript N. gonorrhoeae gene having locus tag NGO1812 andpossibly and putatively encoding major outer membrane protein (porB),and N. gonorrhoeae gene having locus tag NGO1680 and possibly andputatively encoding 50S ribosomal protein L28 (rpmB).

In some embodiments of the instant disclosure a method is described todetect in an N. gonorrhoeae bacteria, a N. gonorrhoeae transcript, whichcomprises

-   quantitatively detecting a transcript expression value of an RNA    marker of N. gonorrhoeae selected from anyone of the RNA markers    of N. gonorrhoeae herein described, in the N. gonorrhoeae following    and/or upon contacting of the N. gonorrhoeae with an antibiotic to    obtain an antibiotic treated transcript expression value for the RNA    marker of .

In some embodiments, the method further comprises detecting whetherthere is a downshift in the transcript expression value of the RNAmarker of N. gonorrhoeae following and/or upon the contacting of the N.gonorrhoeae with the antibiotic by comparing the antibiotic treatedtranscript expression value with an untreated marker expression valueanuntreated marker expression value indicating of the expression of theRNA marker of N. gonorrhoeae in N. gonorrhoeae in absence of antibiotictreatment.

In some embodiments, the reference expression value of the RNA marker ofN. gonorrhoeae in absence of antibiotic treatment is a controltranscript expression value obtained by quantitatively detecting the RNAof N. gonorrhoeae in a control sample not treated with the antibiotic.In some embodiments, the reference transcript expression value of theRNA marker of N. gonorrhoeae is a transcript expression value obtainedby quantitatively detecting the RNA of N. gonorrhoeae in the same sampleprior to treatment with the antibiotic. In some embodiments, thereference transcript expression value of the RNA marker of N.gonorrhoeae is a transcript expression value obtained by quantitativelydetecting the RNA of N. gonorrhoeae at time zero of the RNA expressionof the transcript.

Accordingly, in some embodiments, the method to detect in an N.gonorrhoeae bacteria an N. gonorrhoeae transcripts can be performed by

-   contacting a sample of an isolate or specimen comprising the N.    gonorrhoeae with an antibiotic to obtain an antibiotic treated    sample,-   quantitatively detecting a transcript expression value of a RNA    marker of N. gonorrhoeae herein described in the antibiotic treated    sample at one or more times following and/or upon contacting the    sample with the antibiotic, to provide an antibiotic treated    transcript expression value for the RNA marker of N. gonorrhoeae;    and-   detecting whether there is a downshift of the quantitatively    detected transcript of the RNA marker of N. gonorrhoeae herein    described in the treated sample with respect to an untreated marker    expression valuean untreated marker expression value indicative of    the expression of the RNA marker of N. gonorrhoeae in N. gonorrhoeae    in absence of antibiotic treatment.

In some embodiments an untreated marker expression value indicative ofthe expression of the RNA marker of N. gonorrhoeae in N. gonorrhoeae inabsence of antibiotic treatment is a control transcript expression valueobtained by

-   quantitatively detecting a transcript expression value of the RNA    marker of N. gonorrhoeae herein described in a control sample of the    isolate or specimen comprising the N. gonorrhoeae, to provide a    control transcript expression value of the RNA marker of N.    gonorrhoeae herein described.

In some embodiments, the RNA markers of N. gonorrhoeae herein describedcan be used in a method to perform an antibiotic susceptibility test forN. gonorrhoeae. The method comprises detecting susceptibility to anantibiotic of an N. gonorrhoeae, by quantitatively detecting in a samplecomprising the N. gonorrhoeae a transcript expression value of an RNAmarker of N. gonorrhoeae selected from the RNA markers of an N.gonorrhoeae herein described following and/or upon contacting the samplewith the antibiotic.

In the method to perform an antibiotic susceptibility test for N.gonorrhoeae the quantitatively detecting is performed to obtain anantibiotic treated transcript expression value for the RNA marker of N.gonorrhoeae suitable to detect susceptibility to the antibiotic of theN. gonorrhoeae in the sample.

In some embodiments, the method to perform an antibiotic susceptibilitytest for N. gonorrhoeae further comprises detecting whether there is adownshift of the transcript expression value with respect to theexpression of the transcript in an untreated sample of the same specimenby comparing the detected antibiotic transcript expression value with anuntreated marker expression value indicative of the transcriptexpression in the sample in absence of antibiotic treatment.

In some embodiments, the RNA markers of N. gonorrhoeae herein describedcan be used in a method to detect an RNA marker of susceptibility to anantibiotic in N. gonorrhoeae in a sample comprising the N. gonorrhoeae.The method comprises contacting the sample with the antibiotic to obtainan antibiotic treated sample and quantitatively detecting in theantibiotic treated sample one or more of the RNA markers of N.gonorrhoeae herein described.

In some embodiments, the method to detect an RNA marker ofsusceptibility to an antibiotic in N. gonorrhoeae further comprisesdetecting a downshift of an RNA marker selected from any one of thetranscripts of N. gonorrhoeae genes herein described with respect to anuntreated marker expression value indicative of the expression of theRNA marker of N. gonorrhoeae in N. gonorrhoeae in absence of antibiotictreatment.

The RNA markers of N. gonorrhoeae herein described can be used in amethod to diagnose susceptibility to an antibiotic of a N. gonorrhoeaeinfection in an individual. The method comprises contacting a samplefrom the individual with the antibiotic; and quantitatively detectingexpression by the N. gonorrhoeae in the sample of a marker of antibioticsusceptibility in N. gonorrhoeae selected from any one of thetranscripts of N. gonorrhoeae genes herein described. In the method, thequantitatively detecting is performed following contacting the samplewith the antibiotic. The method further comprises detecting whetherthere is a downshift of the detected transcript presence in theantibiotic sample with respect to an untreated marker expression valueindicative of the expression of the marker of antibiotic susceptibilityin N. gonorrhoeae to diagnose the antibiotic susceptibility of the N.gonorrhoeae infection in the individual.

The RNA markers of N. gonorrhoeae herein described can be used in amethod to detect antibiotic susceptibility of an N. gonorrhoeaebacterium and treat N. gonorrhoeae in an individual. The methodcomprises contacting a sample from the individual with an antibiotic,and quantitatively detecting in the sample expression by the N.gonorrhoeae bacteria of a marker of antibiotic susceptibility selectedfrom any one of the transcripts of N. gonorrhoeae genes hereindescribed. In the method, the quantitatively detecting is performedfollowing and/or upon contacting the sample with the antibiotic.

The method further comprises diagnosing antibiotic susceptibility of N.gonorrhoeae infection in the individual when a downshift in expressionof at least one of the detected markers in the sample is detected incomparison with an untreated marker expression value indicative of theexpression of the at least one of the detected markers in the samplefrom the individual in absence of antibiotic treatment.

The method also comprises administering an effective amount of theantibiotic to the diagnosed individual.

The term “individual” as used herein in the context of treatmentincludes a single biological organism, including but not limited to,animals and in particular higher animals and in particular vertebratessuch as mammals and in particular human beings

In embodiments of the methods of the instant disclosure using any one ofthe N. gonorrhoeae markers herein described, contacting the N. gonorrheacan be performed by adding antibiotics to the microorganism andincubating the sample under certain condition

In particular in some embodiments, the antibiotic for treating thesample herein described can be provided in a sample comprising N.gonorrhoeae at a concentration equal to or the breakpoint MIC for the N.gonorrhoeae, to the antibiotic. In particular, the antibiotic fortreating the sample herein described can be provided at a concentrationlower than the breakpoint MIC for the N. gonorrhoeae strain in thesample, for example 1.5 times (or 1.5X) lower, 2 times (or 2X) lower, 3times (or 3X) lower, 4 times (or 4X) lower, 8 times (or 8X) lower, or 16times (or 16X) lower than the breakpoint MIC for a resistant isolate..In some embodiments, the antibiotic for treating the sample hereindescribed can be provided at a concentration higher than the breakpointMIC for the N. gonorrhoeae strain in the sample, for example 1.5 times(or 1.5X) higher, 2 times (or 2X) higher, 3 times (or 3X) higher, or 4times (or 4X) higher, or 8 times higher (8X) or 16 times higher (or 16X)than the breakpoint MIC for a resistant isolate. The breakpoint MIC ofthe antibiotic for the N. gonorrhoeae strain in the sample, can beobtained from the Clinical & Laboratory Standards Institute (CLSI)guidelines, European Committee of Antimicrobial Susceptibility Testing(EUCAST) or other sources identifiable to a skilled person.

In some embodiments, samples may be treated at several concentrations ofthe antibiotic to measure MIC of an organism and/or to determine if asample contains bacteria with intermediate susceptibility, susceptiblebacteria, or resistant bacteria to the antibiotic of interest. In orderto determine the MIC using the described method, samples can be treatedat multiple concentrations of antibiotic. These concentrations wouldinclude multiple dilutions below the susceptible MIC breakpoint,dilutions between the susceptible and resistant MIC breakpoints(including intermediate breakpoint concentrations), as well as adilution above the resistant MIC breakpoint (see Example 13) Todetermine, degree of susceptibility, the sample would be exposed tothree concentrations of antibiotic: a concentration equal to thesusceptible MIC breakpoint, a concentration equal to the concentrationof the resistant MIC breakpoint, and a concentration equal to theaverage of the maximum and minimum of the intermediate MIC breakpointrange. Susceptibility would then be determined , for example, bymeasuring the slope obtained by fitting a curve or line to the threepoints on the C:T ratio vs treatment concentration plot, and/or bycomparing the relative difference in C:T ratio between the low andintermediate concentration of antibiotic and the difference in CT ratiobetween the intermediate and high concentration, and/or by comparing themagnitude of the value relative to a pre-defined threshold, or acombination of these analyses (see Example 14).

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, the time period ofcontacting the sample with an antibiotic can be up to 5 minutes, 10minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes up to 60 up to90 up to 120 or higher, inclusive of any value therebetween or fractionthereof.

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, the time period ofcontacting the sample with an antibiotic is shorter than the doublingtime of the N. gonorrhoeae strain in the sample. For example, forconditions with N. gonorrhoeae doubling time of 45 minutes, 1 hour, 1.5hours, or 2 hours, antibiotic exposure contacting time could be lessthan the time indicated in Table 6 below

TABLE 6 time of contacting N. gonorrhoeae with antibiotic 45 minutedoubling 60 min doubling 90 min doubling 120 doubling factor Xcontacting time, less than (minutes): 1 45 60 90 120 0.75 33.75 45 67.590 0.5 22.5 30 45 60 0.35 15.75 21 31.5 42 0.25 11.25 15 22.5 30 0.2 912 18 24 0.15 6.75 9 13.5 18 0.1 4.5 6 9 12 0.075 3.375 4.5 6.75 9 0.052.25 3 4.5 6

In methods of the instant disclosure using any one of the N. gonorrhoeaemarkers herein described, incubation of a sample with an antibiotic canbe performed at a temperature such that a physiological response to theantibiotic is generated in N. gonorrhoeae. The contacting is performedtypically in an incubation temperature at 37° C., in an incubationtemperature within the range of 36-38° °C, in an incubation temperaturewithin the range of 35-39° °C.

In methods of the instant disclosure using any one of the N. gonorrhoeaemarkers herein described, the contacting can be performed by addingantibiotics to the microorganism and incubating the sample under certaincondition preferably following and/or upon contacting the sample with atreatment media designed to support physiological processes of N.gonorrhoeae, enable or accelerate DNA replication and translation,maintain cellular uniformity and homogeneity in suspension, and promoteinteraction of the N. gonorrhoeae and antibiotic herein described.

In methods of the instant disclosure using any one of the N. gonorrhoeaemarkers herein described, quantitatively detecting an antibiotic treatedtranscript expression value in the treated sample can be performedfollowing and/or upon contacting the sample with an antibiotic for atime period up to 20 minutes.

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, quantitativelydetecting transcript expression value can be performed by RNA-seq, qPCR,digital PCR, isothermal techniques such as LAMP, digital isothermalamplification methods, or using probes specifically targeting any one ofthe differentially expressed transcripts herein described. Additionaltechniques include microarrays and nanostring^(tm) as will be understoodby a person skilled in the art.

In some embodiments, detecting specific gene expression can be performedat the transcription level by performing RNA sequencing (RNA-seq) andcalculating RNA expression values based on the sequence data.

In some embodiments, the RNA expression values can be calculated astranscripts per million (TPM) as will be understood by a person skilledin the art. To calculate TPM, read counts are first divided by thelength of each gene in kilobases, which gives reads per kilobase (RPK).RPKs for all genes are added and the sum is divided by 1,000,000. Thisgives the “per million” scaling factor. Finally, the RPK value for eachgenes is divided by the “per million” scaling factor to give TPM. [3]

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, quantitativelydetecting a treated gene expression pattern of the transcript can beperformed using probes specifically targeting any one of thedifferentially expressed transcripts herein described.

The term “probe” as described herein indicates a molecule or computersupport tool capable of specifically detect a target molecule such asone of the markers herein described. The wording “specific”“specifically” or “specificity” as used herein with reference to thebinding of a first molecule to second molecule refers to therecognition, contact and formation of a stable complex between the firstmolecule and the second molecule, together with substantially less to norecognition, contact and formation of a stable complex between each ofthe first molecule and the second molecule with other molecules that maybe present. Exemplary specific bindings are antibody-antigeninteraction, cellular receptor-ligand interactions, polynucleotidehybridization, enzyme substrate interactions and additional interactionsidentifiable by a skilled person. The wording “specific” “specifically”or “specificity” as used herein with reference to a computer supportedtool, such as a software indicates a tool capable of identifying atarget sequence (such as the one of a marker herein described) among agroup of sequences e.g. within a database following alignment of thetarget sequence with the sequences of the database. Exemplary softwareconfigured to specifically detect target sequences comprise Primer-3,PerlPrimer and PrimerBlast.

In methods of the instant disclosure using any one of the N. gonorrhoeaetranscripts herein described, treatment of the N. gonorrhoeae bacteriawith a probe and/or antibiotic or with any other reagents functional toperform the related step is performed on samples.

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, the probe specificfor the transcript is selected from a primer having a sequence specificfor the marker, or an antibody specific for the marker.

In particular, probes usable in methods herein described can includeprimers for nucleic acid amplification reactions (such as PCR, LAMP,HAD, RPA, NASBA, RCA, SDA, NEAR, and additional reactions identifiableby a skilled person), including digital single molecule versions ofthese reactions and including real-time versions of these reactions,molecular beacons that include dyes, quenchers, or combinations of dyesand quenchers.

Nucleic acid probes preferably have sequences that complementarily bindto the DNA and/or RNA sequences of the markers described herein, and canbe used to target RNA molecules directly, or DNA molecules that result,for example, from reverse transcription of the target RNA molecules(such molecules may be referred to as cDNA). In embodiments of thepresent disclosure when two polynucleotide strands, sequences orsegments are noted to be binding to each other through complementarilybinding or complementarily bind to each other, this indicate that asufficient number of bases pairs forms between the two strands,sequences or segments to form a thermodynamically stable double-strandedduplex, although the duplex can contain mismatches, bulges and/or wobblebase pairs as will be understood by a skilled person.

The term “thermodynamic stability” as used herein indicates a lowestenergy state of a chemical system. Thermodynamic stability can be usedin connection with description of two chemical entities (e.g. twomolecules or portions thereof) to compare the relative energies of thechemical entities. For example, when a chemical entity is apolynucleotide, thermodynamic stability can be used in absolute terms toindicate a conformation that is at a lowest energy state, or in relativeterms to describe conformations of the polynucleotide or portionsthereof to identify the prevailing conformation as a result of theprevailing conformation being in a lower energy state. Thermodynamicstability can be detected using methods and techniques identifiable by askilled person. For example, for polynucleotides thermodynamic stabilitycan be determined based on measurement of melting temperature T_(m),among other methods, wherein a higher T_(m) can be associated with amore thermodynamically stable chemical entity as will be understood by askilled person. Contributors to thermodynamic stability can include, butare not limited to, chemical compositions, base compositions,neighboring chemical compositions, and geometry of the chemical entity.

In embodiments herein described, primer and/or other nucleic acid probescan be designed to complementarily bind the target marker hereindescribed with methods described in [13].

Probes usable in methods herein described include probes used in guidingCRISPR-based detection of nucleic acids. e.g. CRISPR-associatedprotein-9 nuclease; CRISPR-associated nucleases. An example of aCRISPR-based method is described in references [18] [19] [20]. Suchprobes can be synthesized using naturally occurring nucleotidesincluding deoxyInosine, or include unnatural nucleotides such as lockednucleic acid (LNA). Probes can comprise dyes, quenchers, or combinationsof dyes and quenchers attached to the probe. Hybridization probes,including those used in fluorescent in situ hybridization andhybridization chain reaction. Probes can also comprise electrochemicallyactive redox molecules attached to the probe. Probes can be provided ina dry state. Probes can also include probes bound to beads, such beadsmay be fluorescently labeled. Probes can also include probes bound tonanoparticles, such nanoparticles may include gold nanoparticles. Probescan include probes disposed in arrays of wells with volumes less than 50microliters, and/or wells within plastic substrates. Exemplary probessuitable to be used in methods using any one of the N. gonorrhoeaemarkers herein described comprise probes provided with the commerciallyavailable technology such as the technology of any of the companiesGenProbe, Nanosphere, Luminex, Biofire and additional companiesidentifiable by a skilled person.

In some embodiments, quantitative detection of the marker/transcript isperformed by one or more methods including Northern blotting, NucleaseProtection Assays (NPAs) in situ hybridization, reverse transcriptionpolymerase chain reaction, and qPCR.

In some embodiments, of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, quantitativelydetecting of a marker can be performed by detecting a detectable portionthereof. Exemplary detectable portions comprise to regions of at least14 base pair, at least 16 base pair, at least 18 base pair, at least 19base pair, at least 20 base pair, at least 21 base pair, at least 22base pair, at least 23 base pair, at least 24 base pair, at least 30base pair, at least 40 base pair, at least 50 base pair, at least 60base pair, at least 70 base pair, at least 80 base pair, at least 90base pair, or at least 100 base pair, The specific portion can beidentified by a skilled person based on the length of the transcript tobe detected as will be understood by a skilled person.

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, quantitativelydetecting individual tRNA markers can be performed with quantificationmethods comparable with method used for detection of other RNA markersabove. The secondary structure and multitude of base modificationsprevalent on tRNA often makes reverse transcription inefficient and thusa variety of modified reverse transcription steps can be used. These caninvolve more flexible reverse transcriptases (RTs) like group II intronreverse transcriptase[21] [22].

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, the methods comprisedetecting whether there is a shift in the transcript expression of themarkers, in a sample treated with an antibiotic with respect to a samplenot treated with antibiotic.

In particular, in embodiments of the methods of the instant disclosureusing any one of the N. gonorrhoeae markers herein described, themethods comprise detecting whether there is a downshift of a detectedpresence in N. gonorrhoeae of a N. gonorrhoeae marker followingtreatment with antibiotic with respect to an untreated marker expressionvalue indicative of the expression in N. gonorrhoeae of the one or moreN. gonorrhoeae marker in absence of antibiotic treatment.

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers, the reference expression value is acontrol transcript expression value of the RNA marker of N. gonorrhoeaedetected in a control sample of the specimen, and detecting whetherthere is a downshift can be performed by comparing the antibiotictreated transcript expression value with respect to the controltranscript expression value of the RNA marker of N. gonorrhoeae in acontrol sample of the specimen.

Therefore, in some embodiments of the methods of the instant disclosureusing any one of the N. gonorrhoeae markers, the reference expressionvalue indicative of the expression of the RNA marker of N. gonorrhoeaein absence of antibiotic treatment is a control transcript expressionvalue obtained by quantitatively detecting the RNA of N. gonorrhoeaemarker in a control sample not treated with the antibiotic.

A shift in the expression of the markers can be determined bycalculating differential gene expression levels (C:T ratios) asdescribed above in connection with methods to identify a marker ofantibiotic susceptibility.

In particular in methods of the instant disclosure using any one of theN. gonorrhoeae markers, the methods can comprise for a specimencomprising N. gonorrhoeae (e.g. from an individual).

-   providing a treated N. gonorrhoeae sample treated with the    antibiotic and a control N. gonorrhoeae sample not treated with the    antibiotic,-   quantitatively detecting a control N. gonorrhoeae gene expression    value C for a N. gonorrhoeae marker gene in the control N.    gonorrhoeae sample,-   quantitatively detecting a treated N. gonorrhoeae gene expression    value T for the N. gonorrhoeae marker gene in the treated N.    gonorrhoeae sample, and-   providing a N. gonorrhoeae (C:T) value for the N. gonorrhoeae marker    gene by dividing C for the N. gonorrhoeae marker gene by T for    the N. gonorrhoeae marker gene detected in the sample, and-   detecting differential expression of the N. gonorrhoeae marker gene    based on the N. gonorrhoeae C:T value:

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, the marker comprisesmore than one marker.

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, the N. gonorrhoeaebacteria is selected from any strain of N. gonorrhoeae including itsgenetic variants.

In some embodiments, the C:T ratio can be provided by RPKM (reads perkilobase per million mapped reads). The use of RPKM and comparison toTPM is described for example in Wagner et al 2012 [3]. In someembodiments the C:T ratio is provided by FPKM (fragments per kilobaseper million), the use of FPKM is described for example in Conesa et al.2016 [4]. These units normalize for sequencing depth and transcriptlength. In some embodiments RPM (reads per million mapped reads; RPMdoes not normalize for transcript length) or raw sequencing read countscan be used. The related methods are identifiable by a skilled personupon reading of the present disclosure.

In methods of the instant disclosure using any one of the N. gonorrhoeaemarkers, the differential expression of the N. gonorrhoeae marker can beexpressed in accordance with a fold change approach in view of the C:Tratios identifiable by a skilled person upon reading of the presentdisclosure. In particular in the fold-change approach, a gene isconsidered to be differentially expressed if the ratio of the markerexpression level between the antibiotic treated and untreated conditionsexceeds a certain threshold, for example, 1.5-fold, twofold orthreefold, or 4-fold or 5-fold change.

Accordingly, in some embodiments of the methods of the instantdisclosure using any one of the N. gonorrhoeae markers at least 1.2-foldmagnitude of fold change is considered as a shift. In some embodiments,contacting the sample with an antibiotic results the markers a 1.5 foldchange or 2-fold or 4-fold change up to 6-fold change within the first 5minutes of contact. Increasing the antibiotic exposure time can furthershift the fold-change value.

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers, the downshift of the transcriptpresence is at least 2-fold, 4-fold or is 6-fold or higher.

In preferred embodiments, the (C:T) value of an N. gonorrhoeae markercan be adjusted to reduce the impact of biological variability and/ortechnical variability in the C:T detection, more preferably of bothbiological and technical variability.

Accordingly, in some embodiments, any one of the methods of the instantdisclosure using any one of the N. gonorrhoeae markers herein describedcan further comprise normalizing the antibiotic treated transcriptexpression value, the control transcript expression value and/or therelated ratio, before detecting whether there is a downshift inantibiotic treated sample with respect to the untreated sample.

In particular, in some embodiments, at least one of the antibiotictreated transcript expression value and the control transcriptexpression value are normalized before providing a C:T ratio. In someembodiments, the C:T ratio of the antibiotic treated transcriptexpression value and the control transcript expression value isnormalized using reference measurements.

The normalization can be performed by dividing the antibiotic treatedtranscript expression value, the control transcript expression valueand/or the related ratio, by a reference measurement of RNA, DNA, cellnumber, number of samples, effective amount of sample used and/or arelated ratio in a control and in a treated sample, according toapproaches indicated for methods to identify markers of antibioticsusceptibility of the disclosure.

In particular, in some of these embodiments, the quantitativelydetecting can be performed at a plurality of times following and/or uponcontacting the sample, and/or under several conditions following and/orupon contacting the sample. For example in some of these embodiments,the antibiotic can be added at different concentrations. Also, in someof those embodiments adding the antibiotic can be performed in thetreated N. gonorrhoeae sample throughout incubation or at set intervalsduring incubation to increase or decrease the physiological response ofthe N. gonorrhoeae to the antibiotic. Also in some of those embodiments,the quantitatively detecting can be performed at various times includingtime zero (for example, immediately prior or immediately afterantibiotic treatment) of the transcript expression in the sample. Insome of those embodiments, the quantitatively detecting can be performedat various temperatures and/or in multiple samples. In theseembodiments, normalization can be performed by dividing the detectedexpression value and/or the related ratio between treated and controlsamples by the volume of samples or other reference measurements, suchas the expression value of a reference RNA, level of DNA, cell numbers,as well as other reference parameters.

The control transcripts and related method of identification describedin the method to identify markers of the present disclosure apply to theinstant methods as will be understood by a skilled person.

Preferably, control transcripts are selected so this C:T ratio has lowtechnical and biological variability, for example described by standarddeviation with value of less than 0.5, less than 0.4, less than 0.3,less than 0.2, less than 0.1. In some embodiments, high-abundancetranscripts (for example, transcripts in the top 10% of most expressedtranscripts) are used to achieve low technical variability. Preferably,control transcripts are selected so this C:T ratio has low biologicalvariability. Transcripts with high expression not affected by theantibiotic treatment are good candidates for control transcripts withlow biological variability. For mRNA high expression level is obtainedwith more than 10 copies per cell or equivalent parameter in view of themethod of measurement (for example RNAseq can have preferred expressionlevels for detection are TPM > 100 for any transcript and “highexpression” being TPM > 100,000 (greater than 3000 copies/cell).

In some embodiments, a control transcript can be selected by providing apool of isolates with known susceptibility; for each of these isolates,measuring a CT ratio of each transcript; and selecting as the controltranscripts the transcripts with a CT ratio that is substantially thesame in the pool of isolates between the susceptible isolates and theresistant isolates. The pool of isolates can be obtained from CDCAntimicrobial Resistance Isolate Bank. and/or from clinical collectionsof isolates.

Alternatively, the control transcript can be selected by measuring a CTratio of each transcript in a strain subject to the antibioticsusceptibility test, i.e. with unknown susceptibility, and selecting asthe control transcript the transcript with a CT ratio close to one, i.e.transcripts with expression not affected by the antibiotic treatment.Preferably, the control transcripts have a high expression level (e.g.with a TPM >10,000). Exemplary control transcripts comprise thetranscript listed in Table 1.

In some embodiments, the control transcript can be a ribosomal RNA,including 23S rRNA, 16S rRNA, 5S rRNA and other RNA component ofribosome.

In some embodiments, 16S rRNA or 23 rRNA are used as reference RNA fornormalization (see e.g. Table 2 of the instant disclosure).

In some embodiments of the fold-change approach, a gene is considered tobe differentially expressed if the ratio of the normalized markerexpression level between the antibiotic treated and untreated conditionsexceeds a certain threshold, for example, 1.5 fold, twofold orthreefold, or 4-fold or 5-fold change, wherein normalization can beperformed with any of the methods herein described.

In some embodiments of any one of the methods of the instant disclosureusing any one of the N. gonorrhoeae markers herein described, detectingwhether there is a downshift can be performed by comparing theantibiotic treated transcript expression value of the RNA marker of N.gonorrhoeae with the expression value in the treated sample of abiomarker of the expression of the RNA marker of N. gonorrhoeae todetect the downshift. In particular, a biomarker of the expression canbe any molecule and in particular a transcript, whose expression, undercontrol conditions, has been previously shown to be correlated with theexpression of the RNA marker of N. gonorrhoeae, preferably for aplurality of strains. In some embodiments, a downshift of expression ofthe RNA marker is detected when the ratio of expression of this markerto the expression of the biomarker of the expression in the treatedsample is statistically significantly different than the range of ratiosexpected based on the analysis correlation of expression of these twomarkers under control conditions.

In some embodiments any one of the methods of the instant disclosureusing any one of the N. gonorrhoeae markers herein described, the N.gonorrhoeae marker is a plurality of N. gonorrhoeae markers. In thoseembodiments the quantitative detection of the related expression can beperformed by detecting global gene expression, or patterns of geneexpression, in the tested samples for the plurality of the N.gonorrhoeae markers, as will be understood by a skilled person.

In methods of the instant disclosure using any one of the N. gonorrhoeaemarkers herein described, the sample can be provided from urine, swab,genital swab, throat swab, urethral swab, cervical swab, vaginal swab,oropharyngeal swab, throat swab, and rectal swabs. For urine sample, thepreferable amount is between 1 ul and 10 ml. If the sample is providedas in swabs, the swab can be placed in an elution buffer to elutebacterial target cells from the swab. Samples can also include bacterialculture samples, for example, those grown on solid media such aschocolate agar, or grown in liquid culture such as Hardy FastidiousBroth (HFB).

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, the sample can bepretreated to enrich RNA or a N. gonorrhoeae by removal of human RNA orRNA of other microorganisms. The removal of human RNA can be performedvia hybridization to beads or columns with probes specific for humanRNA. The removal of human RNA can also be performed via selective lysisof human cells and degradation of released human RNA. The sample mayalso be pretreated to enrich tRNA via size selection.

In general, in embodiments of the methods of the instant disclosureusing any one of the N. gonorrhoeae markers herein described, enrichinga sample can be performed with methods and approaches described for themethods to identify an antibiotic susceptibility marker of thedisclosure.

In some embodiments of the methods of the instant disclosure using anyone of the N. gonorrhoeae markers herein described, the sample can bestored until sample preparation and analysis, for example at roomtemperature, 4° C., -20° C., or -80° C., as appropriate, identifiable bythose skilled in the art. When biological specimens are stored, ideallythey remain equivalent to freshly-collected specimens for the purposesof analysis. In some embodiments, of the methods of the instantdisclosure using any one of the N. gonorrhoeae markers herein described,the sample can be pre-incubated with growth media for a short period oftime to increase the number of viable bacterial cells or to increase thelevel of RNA expression in such cells. The temperature and media forsuch pre-incubation can be performed as described herein for incubation.The duration of such pre-incubation can range, for example, from 5minutes to 20 minutes to 1 hour to 2 hours.

In some embodiments of the instant disclosure wherein the microorganismis N. meningitidis, markers are expected to be selected from atranscript of a N. meningitidis gene based on the fact that Neisseriameningitidis also lacks the SOS response [23] ([24] (and [25] or acorresponding cDNA.

In particular, markers are expected to be selected from a transcript ofa N. meningitidis gene comprise the ones listed in Table 7

TABLE 7 List of exemplary marker genes expected to be differentiallyexpressed between an untreated sample and a sample treated withantibiotics Marker DNA(+)strand cDNA strand RNA PorBNC_003112.2:2157529-2158524 Neisseria meningitidis MC58 SEQ ID NO:154 inANNEX C SEQ ID NO:155 in ANNEX C SEQ ID NO:156 in ANNEX C rpmBNC_003112.2:332567-332800 Neisseria meningitidis MC58 - on the (-)strandSEQ ID NO:157 in ANNEX C SEQ ID NO:158 in ANNEX C SEQ ID NO:159 in ANNEXC

In some embodiments, markers according to the instant disclosure canhave a sequence identity of at least 80%, or 90%, up to 100% of themarkers listed in Table 7. In particular markers of the instantdisclosure can have sequence identity of 93%, 94%, 95%, 96%, 97%, 98%,or 99% of the sequences indicated in Table 7.

The RNA marker of N. meningitidis and/or corresponding cDNA can be usedto detect a transcript of N. meningitidis., perform an antibioticsusceptibility test for N. meningitidis, detect an RNA marker ofsusceptibility to an antibiotic in N. meningitidis, diagnosesusceptibility to an antibiotic of a N. meningitidis infection in anindividual, and/or detect antibiotic susceptibility of an N.meningitidis bacterium and treat N. meningitidis in an individual, withmethods and systems comprising the features indicated in any one of thethird to the eighth aspect of the summary section and related portion ofthe detailed description of the instant disclosure in connection with N.gonorrhoeae transcripts and/or corresponding cDNA and their use inmethods and systems related to the N. gonorrhoeae microorganism.

Methods of the present disclosure using any one of the N. gonorrhoeaetranscripts and/or N. meningitidis herein described, can be performedwith a corresponding system comprising at least one probe specific for atranscript herein described and/or or probe specific for cDNA atranscript herein described, and reagents for detecting the at least oneprobe. The at least one probe and reagents are included in the systemfor simultaneous combined or sequential use in any one of the methods ofthe present disclosure using any one of the N. gonorrhoeae transcriptsherein described.

In particular, in the instant disclosure a system is described forperforming at least one of the methods herein described to detect an N.gonorrhoeae transcript, to detect antibiotic susceptibility of N.gonorrhoeae bacteria, to perform an antibiotic susceptibility test foran N gonorrhoeae, and/or to diagnose and/or treat N. gonorrhoeae in anindividual. The system comprises at least one probe specific for atranscript selected from any one of the transcripts of N. gonorrhoeaegenes herein described, and/or a probe specific for cDNA a transcriptherein described, and reagents for detecting the at least one probe.

In some embodiments of the system herein described the system comprisesat least one probe specific for a transcript, and/or probe specific fora corresponding cDNA of said transcript, selected from at least one of atranscript of N. gonorrhoeae gene having locus tag NGO1812 and encodingmajor outer membrane protein (porB), a transcript of N. gonorrhoeae genehaving locus tag NGO1680 and encoding 50S ribosomal protein L28 (rpmB),a transcript of N. gonorrhoeae gene having locus tag NGO1291 andencoding transcriptional regulator (yebC)a transcript of N. gonorrhoeaegene having locus tag NGO1673 and encoding type IV pilus assemblyprotein(pilB), a transcript of a transcript of N. gonorrhoeae genehaving locus tag NGO0592 and encoding trigger factor (tig) and atranscript of N. gonorrhoeae gene having locus tag NGO0340 and encodingcysteine synthase A (cysK).

In some embodiments of the system herein described the system comprisesat least one probe specific for a transcript and/or a correspondingcDNA, which comprises or is at least one of a transcript N. gonorrhoeaegene having locus tag NGO1812 and annotated as encoding major outermembrane protein (porB), and/or a corresponding cDNA and N. gonorrhoeaegene having locus tag NGO1680 and annotated as encoding 50S ribosomalprotein L28 (rpmB) and/or a corresponding cDNA.

In some embodiments of the system herein described the system comprisesprimers configured to specifically hybridizes with the transcript and/ora corresponding cDNA. In some of these embodiments the system comprisesa probe specific for a transcript of N. gonorrhoeae gene having locustag NGO1812, the probe comprises a pair of primers having sequenceGCTACGATTCTCCCGAATTTGCC (SEQ ID NO: 160) (CCGCCKACCAAACGGTGAAC (SEQ IDNO: 161), a probe specific for a transcript of N. gonorrhoeae genehaving locus tag NGO1680 the probe comprises a pair of primers havingsequence TTGCCCAACTTGCAATCACG (SEQ ID NO: 162) and AGCACGCAAATCAGCCAATAC(SEQ ID NO: 163). a probe specific for a transcript of N. gonorrhoeaegene having locus tag NGO1291 the probe comprises a pair of primershaving sequence GCTTTGGAAAAAGCAGCCG (SEQ ID NO: 164) andGGTTTTGTTGTCGGTCAGGC (SEQ ID NO: 165), a probe specific for a transcriptof N. gonorrhoeae gene having locus tag NGO1673, the probe comprises apair of primers having sequence GACTTTTGCCGCTGCTTTG (SEQ ID NO: 166) andGCGCATTATTCGTGTGCAG (SEQ ID NO: 167), a probe specific for a transcriptof N. gonorrhoeae gene having locus tag NGO0592 the probe comprises apair of primers having sequence AAAGCCTTGGGTATTGCGG (SEQ ID NO: 168) andTGACCAAAGCAACCGGAAC (SEQ ID NO: 169). and/or a probe specific for atranscript of N. gonorrhoeae gene having locus tag NGO0340 the probecomprises a pair of primers having sequence GAGGCTTCCCCCGTATTGAG (SEQ IDNO: 170) and TTCAAAAGCCGCTTCGTTCG (SEQ ID NO: 171).

In some embodiments, the systems of the disclosure to be used inconnection with methods herein described using any one of the N.gonorrhoeae transcripts herein described, the system further comprises aprobe specific for a reference RNA and/or a corresponding cDNA. In someof these embodiments, the reference RNA is N. gonorrhoeae 16S rRNA theand the probe comprises a pair of primers having sequence the probecomprises a pair of primers having sequence ACTGCGTTCTGAACTGGGTG (SEQ IDNO: 172) and GGCGGTCAATTTCACGCG (SEQ ID NO: 173). In some of theseembodiments, the control transcript is N. gonorrhoeae 23S rRNA and theprobe comprises a pair of primers having sequence the probe comprises apair of primers having sequence GCATCTAAGCGCGAAACTCG (SEQ ID NO: 174),and CCCCACCTATCAACGTCCTG (SEQ ID NO: 175).

In some embodiments, the systems of the disclosure to be used inconnection with methods herein described using any one of the N.gonorrhoeae transcripts herein described or cDNA of any one of the N.gonorrhoeae transcripts herein described the system can further comprisean antibiotic formulated for administration to a sample in combinationwith the at least one probe.

In some embodiments, the systems of the disclosure to be used inconnection with methods herein described using any one of the N.gonorrhoeae transcripts herein described and/or cDNA of any one of theN. gonorrhoeae transcripts herein described, the system furthercomprises an antibiotic formulated for administration to an individualin an effective amount to treat an N. gonorrhoeae infection in theindividual.

In some embodiments, the systems of the disclosure to be used inconnection with methods herein described using any one of the N.gonorrhoeae transcripts herein described, the reagents comprise RNAextraction kit and amplification mix. The system may also include one ormore antibiotics and/or exposure media with or without the antibiotics.The system can also include reagents required for preparing the sample,such as one or more of buffers e.g. lysis, stabilization, binding,elution buffers for sample preparation, enzyme for removal of DNA e.g.DNase I, and solid phase extraction material for sample preparation.,reagents required for quantitative detection such as intercalating dye,reverse-transcription enzyme, polymerase enzyme, nuclease enzyme (e.g.restriction enzymes; CRISPR-associated protein-9 nuclease;CRISPR-associated nucleases as described herein) and reaction buffer.Sample preparation materials and reagents may include reagents forpreparation of RNA and DNA from samples, including commerciallyavailable reagents for example from Zymo Research, Qiagen or othersample preparations identifiable by a skilled person. The system canalso include means for performing RNA quantification such as one or moreof: container to define reaction volume, droplet generator for digitalquantification, chip for digital detection, chip or device formultiplexed nucleic acid quantification or semiquantification, andoptionally equipment for temperature control and detection, includingoptical detection, fluorescent detection, electrochemical detection.

In some embodiments, the system can comprise a device combining allaspects required for an antibiotic susceptibility test.

The systems herein disclosed can be provided in the form of kits ofparts. In kit of parts for performing any one of the methods hereindescribed, the probes and the reagents for the related detection can beincluded in the kit alone or in the presence of one or more antibioticas well as any one of the RNA markers, corresponding cDNA and/or probesfor one or more reference RNAs and/or corresponding cDNAs. In kit ofparts for the treatment of an individual the probes and reagents for therelated detection can be comprised together with the antibioticformulated for administration to the individual as well as additionalcomponents identifiable by a skilled person.

In a kit of parts, the probes and the reagents for the relateddetection, antibiotics, RNA markers, and/or reference RNA and additionalreagents identifiable by a skilled person are comprised in the kitindependently possibly included in a composition together with suitablevehicle carrier or auxiliary agents. For example, one or more probes canbe included in one or more compositions together with reagents fordetection also in one or more suitable compositions.

Additional components can include labeled polynucleotides, labeledantibodies, labels, microfluidic chip, reference standards, andadditional components identifiable by a skilled person upon reading ofthe present disclosure.

The terms “label” and “labeled molecule” as used herein refer to amolecule capable of detection, including but not limited to radioactiveisotopes, fluorophores, chemiluminescent dyes, chromophores, enzymes,enzymes substrates, enzyme cofactors, enzyme inhibitors, dyes, metalions, nanoparticles, metal sols, ligands (such as biotin, avidin,streptavidin or haptens) and the like. The term “fluorophore” refers toa substance or a portion thereof which is capable of exhibitingfluorescence in a detectable image. As a consequence, the wording“labeling signal” as used herein indicates the signal emitted from thelabel that allows detection of the label, including but not limited toradioactivity, fluorescence, chemoluminescence, production of a compoundin outcome of an enzymatic reaction and the like.

In embodiments herein described, the components of the kit can beprovided, with suitable instructions and other necessary reagents, inorder to perform the methods here disclosed. The kit will normallycontain the compositions in separate containers. Instructions, forexample written or audio instructions, on paper or electronic supportsuch as tapes, CD-ROMs, flash drives, or by indication of a UniformResource Locator (URL), which contains a pdf copy of the instructionsfor carrying out the assay, will usually be included in the kit. The kitcan also contain, depending on the particular method used, otherpackaged reagents and materials (i.e. wash buffers and the like).

Further details concerning the identification of the suitable carrieragent or auxiliary agent of the compositions, and generallymanufacturing and packaging of the kit, can be identified by the personskilled in the art upon reading of the present disclosure.

EXAMPLES

The methods and system herein disclosed are further illustrated in thefollowing examples, which are provided by way of illustration and arenot intended to be limiting.

Example 1: Microorganisms’ Exposure to Antibiotic

Antibiotic susceptible and resistant clinical isolates were obtainedfrom the University of California, Los Angeles, Clinical MicrobiologyLaboratory.

Isolates were plated from glycerol stocks onto Chocolate Agar plates andgrown in static incubation overnight (37° C., 5% CO₂). Cells werere-suspended in Hardy Fastidious Broth (HFB) and incubated for 45 min(37° C., 5% CO₂) with shaking (800 rpm) to an OD₆₀₀ between 1 and 5.Cultures were diluted (5X) into HFB. Each isolate culture was split into“treated” and “control” tubes.

Ciprofloxacin was added to the “treated” tubes (final concentration of0.5 µg/mL) and water was added to the “control” tubes; cultures wereincubated (static; 37° C., 5% CO₂) for 15 min. During incubation,samples were collected for RNA sequencing at 5, 10, and 15 min (300 µLaliquot of sample was mixed into 600 µL of Qiagen RNA Protect Reagent(Qiagen, Hilden, Germany) for immediate RNA stabilization).

In addition, a sample was collected for RNA sequencing immediatelybefore ciprofloxacin was added.

To quantify CFU, the sample at t = 15 min was serially diluted (10x),plated on a Chocolate Agar plate, and incubated overnight (37° C., 5%CO₂).

Example 2: Microorganisms’ Exposure to Antibiotic

Antibiotic susceptible and resistant clinical isolates were obtainedfrom the N. gonorrhoeae panel of the CDC Antimicrobial ResistanceIsolate Bank. Isolates were plated from glycerol stocks onto ChocolateAgar plates and grown in static incubation overnight (37° C., 5% CO₂).Cells were re-suspended in pre-warmed HFB + 5 mM sodium bicarbonate andincubated for 30 min (37° C., 5% CO₂) with shaking (800 rpm) to an OD₆₀₀between 1 and 5. Cultures were diluted (100X) into HFB + 5 mM sodiumbicarbonate.

Each isolate culture was split into treated (0.5 µg/mL finalconcentration of ciprofloxacin) and control (water instead ofantibiotic) samples. Samples were incubated at 37° C. for 10 min on astatic hot plate. A 90 µLaliquot of each sample was placed into 180 µLofQiagen RNA Protect Reagent for immediate RNA stabilization. A 5µLaliquot of each sample was plated onto a Chocolate Agar plate andincubated overnight (37° C., 5% CO₂) as a control for the exposureexperiments. If the expected growth phenotypes (i.e. resistant = growth;susceptible = no growth) were not observed for any single sample in theplating control, the exposure experiment was repeated for the set ofsamples.

From the 50 total isolates available from the N. gonorrhoeae panel ofthe CDC Antimicrobial Resistance Isolate Bank, 49 were used in thisstudy. One isolate was excluded from this study because it is suspectedthat it had been contaminated; N. gonorrhoeae porB primer amplificationwas not detected using qPCR.

Example 3: RNA Sequencing and Analysis

RNA was extracted using the Enzymatic Lysis of Bacteria protocol of theQiagen RNeasy Mini Kit and processed according to the manufacturer’sprotocol. DNA digestion was performed during extraction using the QiagenRNase-Free DNase Set.

The quality of extracted RNA was measured using an Agilent 2200TapeStation (Agilent, Santa Clara, CA, USA). Extracted RNA samples wereprepared for sequencing using the NEBNext Ultra RNA Library Prep Kit forIllumina (New England Biolabs, Ipswitch, MA, USA) and the NEBNExtMultiplex Oligos for Illumina. Libraries were sequenced at 50 singlebase pair reads and a sequencing depth of 10 million reads on anIllumina HiSeq 2500 System (Illumina, San Diego, CA, USA) at the Millardand Muriel Jacobs Genetics and Genomics Laboratory, California Instituteof Technology. Raw reads from the sequenced libraries were subjected toquality control to filter out low-quality reads and trim the adaptorsequences using Trimmomatic (version 0.35).

The reads were aligned to the FA 1090 strain of N. gonorrhoeae (NCBIReference Sequence: NC_002946.2) using Bowtie2 (version 2.2.5) andquantified using the Subread package (version 1.5.0-p1). A pseudocountof 1 was added to the gene quantification; gene expression was definedin transcripts per million (TPM).

Example 4: Marker Selection Based on C:T Ratio

For each gene, the C:T ratio was defined as the gene expression (TPM) inthe control sample divided by the gene expression (in TPM) in thetreated sample. The -log₂(C:T) was plotted against the -log₂(expressionin TPM) for all genes. To identify genes that were differentiallyexpressed between control and treated samples, a threshold ofsignificance was defined.

The threshold of significance was calculated from the C:T ratios at t =0 for the biological replicates that were sequenced (three susceptibleand three resistant isolates). For each of the six gene expressiondatasets (one for each isolate), a negative exponential curve was fit tothe outer edge of each plot and then the curves were averaged from allsix datasets.

Finally, a 90% confidence interval was added to the average curve byassuming a Gaussian fit for the error distribution, which is thethreshold of significance. Genes with a -log₂(C:T) value above or belowthe upper and lower thresholds were identified as differentiallyexpressed. Genes that were differentially expressed consistently (eitheralways above or always below the thresholds) among the three susceptibleisolates and were not differentially expressed among the three resistantisolates were defined as candidate markers.

Example 5: Copies/Cell Measurements

To measure copies per cell using RNA sequencing data, 2uL of (1/1000dilution) ERCC RNA Spike-In Mix (Thermo Fisher Scientific, Waltham, MA,USA) was added to the lysis buffer in the RNeasy Mini Kit to eachindividual sample. The number of copies of each ERCC transcript in thesample was calculated, by accounting for dilution and multiplying byAvogadro’s number (manufacturer’s concentrations were reported inattomoles/µL). The relationship between log₂(ERCC copies added) againstlog₂(gene expression in TPM) was plotted and a linear regression in theregion of linearity was performed. The linear regression was used toconvert TPM values to total RNA copies in each sample. Finally, usingthe CFU measured for each sample from plating (described in the“Antibiotic exposure for RNA sequencing” section), the total RNA copieswere converted to copies per cell.

Example 6: Validation With Droplet Digital PCR (dPCR)

Primers were designed for candidate markers using Primer-BLAST[13] andprimer alignments were verified using SnapGene. Expression of candidatemarkers was quantified using the Bio-Rad QX200 droplet dPCR system(Bio-Rad Laboratories, Hercules, CA, USA). The concentration of thecomponents in the dPCR mix used in this study were as follows: 1×EvaGreen Droplet Generation Mix (Bio-Rad), 150U/mL WarmStart RTx ReverseTranscriptase, 800U/mL RiboGaurd RNase Inhibitor, 500 nM forward primer,and 500 nM reverse primer. The RNA extraction comprised 5% of the finalvolume in the dPCR mix.

For each isolate, candidate marker expression was quantified in thecontrol and treated samples and the fold-change difference (C:T ratio)was calculated. To account for potential differences between the controland treated samples that could arise from experimental variability andextraction efficiency, ribosomal RNA (rRNA) was used as an internalcontrol.

From the sequencing data, it was found that rRNA was not affected byantibiotic exposure in the time frame of this study and showed very lowvariability. The 16S rRNA in the control was therefore also quantified,samples were treated by dPCR and an rRNA C:T ratio was calculated. TheC:T ratio of each marker was normalized with the rRNA C:T ratio. AlldPCR C:T ratios reported in the example section of the disclosure arethe normalized C:T ratios.

Example 7: Temporal Shifts in Global Gene Expression Upon AntibioticExposure

RNA-seq was used to study the transcriptome response of susceptible andresistant isolates of N. gonorrhoeae after 5, 10, and 15 min ofciprofloxacin exposure (FIG. 1 ). Each clinical isolate was initiallysplit into two tubes, where one tube was exposed to the antibiotic (+)and the other served as the control with no antibiotic exposure (-).Samples were collected for RNA-seq prior to antibiotic exposure (timezero) and every 5 min for 15 min. The fold change in gene expression wascalculated between the control and treated samples, which is defined asthe control:treated ratio (C:T ratio).

Genes that demonstrated significant fold-change differences between thesusceptible and resistant isolates were identified as differentiallyexpressed. To account for biological variability, three pairs ofsusceptible and resistant isolates were used in this study to identifymarkers. Candidate markers were selected from the pool of differentiallyexpressed genes and were validated using droplet dPCR (see Examples 4and 6).

Global shifts were observed in RNA expression in susceptible isolates inas early as 5 min after antibiotic exposure (FIG. 2A). The distributionof fold changes in gene expression levels (C:T ratios) indicated globalshifts toward negative log₂ fold-change values (downregulation). Themagnitude of fold change at which most genes were distributed wasapproximately 2-fold. The tail of the distribution illustrates that afew genes responded to antibiotic exposure with changes as large as6-fold within 5 min. Increasing the antibiotic exposure time furthershifted the distribution to larger negative log₂ fold-change values. Thetranscriptional response in resistant isolates was tightly distributedaround negative log₂ zero values at all time points, indicating that thetranscriptome did not significantly respond to antibiotic in theresistant isolates (FIG. 2A).

To identify genes that were differentially expressed between control andtreated samples, a threshold of significance was defined (FIG. 2B). Thethreshold of significance took into account technical variability andwas calculated from the C:T ratios at t = 0 min of all biologicalreplicates that had RNA sequenced (three susceptible and three resistantisolates). For each of the six gene expression datasets (one for eachisolate), the -log₂(C:T ratio) was plotted against the -log₂(expression)for all genes and a negative exponential curve was fit to the outer edgeof each plot.

The curves were then averaged from all six datasets and added a 90%confidence interval to the average curve by assuming a Gaussian fit forthe error distribution, which was defined as the threshold ofsignificance. Genes with a -log₂(C:T ratio) value above or below theupper and lower thresholds were identified as differentially expressed.Downregulated genes (fold changes below the significance threshold)appeared as early as 5 min after antibiotic exposure (blue dots, FIG.2B). Two upregulated genes (above the significance threshold) appearedafter 10 min of exposure (orange dots, FIG. 2B).

A key aim of this study was to identify RNA markers that would yield ameasurable response after only a short antibiotic exposure (less orequal to 15 min) to ensure this approach can fit within the requiredtimescale for a rapid AST. It is possible that longer exposure timescould provide additional insight into the biological response of N.gonorrhoeae to ciprofloxacin, but this was not the focus of this study.Furthermore, the short exposure times potentially introduce a biastoward transcripts present at low abundance when evaluating fold change.

For transcripts present at high abundance to display the same foldchange, a substantially higher number of mRNA molecules must betranscribed, which would require longer timescales. As an example, a4-fold change from 1 to 4 transcripts requires 3 additional mRNA to beproduced, whereas a 4-fold change from 20 to 80 requires 60 mRNA to betranscribed. This bias also holds true in downregulation, where mRNAcontinues to be transcribed in the control samples, whereas transcriptlevels drop in treated samples due to degradation of RNA, and/or areduction in rate of transcription.

Example 8: Selection of Candidate Markers That Are Consistent inResponse and Abundant

RNA expression in response to antibiotics can be heterogeneous amongdifferent isolates of the same species[26]; thus, it is important toselect candidate markers from differentially expressed genes thatrespond consistently across isolates of N. gonorrhoeae.

To identify these markers, three different pairs of susceptible isolates(minimum inhibitory concentrations (MICs) <= 0.015microg/mL) andresistant isolates (MICs 2.0 microgram/mL, 4.0 microgram/mL, and 16.0microgram/mL) were exposed to ciprofloxacin for 15 min and extracted RNAfor sequencing (see workflow in FIG. 1 ).

The nature of the transcriptional response of N. gonorrhoeae toantibiotic exposure was a global downregulation in transcript levels. Inparticular, 181, 41, and 410 differentially expressed genes were foundin susceptible isolates 1, 2, and 3, respectively (FIG. 3A).

Among the differentially expressed genes, 38 genes respondedconsistently across the three pairs of susceptible and resistantisolates (i.e. responses overlapped in all three susceptible isolatesand were not responsive in all three resistant isolates) (see FIG. 6 ).

Among the 38 candidate markers, 15 were ribosomal proteins (includingone of the top markers, rpmB), which play a prominent role in assemblyand function of the ribosomes and are essential for cell growth.Mutations in ribosomal proteins have been reported to confer resistanceto different classes of antibiotics[27].

These 38 genes spanned a variety of biochemical functions in the cell.Six candidate transcript markers were selected for further analysisbased on the following criteria: (1) high fold change; (2) highexpression levels (>75 transcripts per million, TPM); and (3)representative of different biochemical pathways.

The selected candidate markers were: porB (membrane protein), rpmB(ribosomal protein), tig (molecular chaperone), yebC (transcriptionalregulator), pilB (pilus assembly ATPase), and cysK (cysteine synthase).Among the candidate marks, all exhibited downregulation in response tociprofloxacin.

The candidate marker with the highest abundance and largest fold changeupon antibiotic exposure was porB, which is a membrane channel formingprotein and the site of antibiotic influx into the cell[28]. porB is aporin protein responsible for uptake of small nutrients and the site ofantibiotic influx into the cell. The expression of porins is highlyregulated in response to environmental stimuli[29]. Reducingpermeability to decrease intracellular antibiotic concentration is aknown mechanism for bacteria to confer antibiotic resistance[27]. Thedownregulation of porB observed in this study can be attributed to ahalt in growth processes caused by ciprofloxacin damage and possibly anattempt to reduce influx of antibiotic.

A high level of gene expression was one of the criteria for selection ofcandidate markers from the sequencing data. High expression of candidatemarkers is not only important for sensitivity and limits of detection,as has been previously demonstrated in AST methods based onquantification of DNA replication[30], but is particularly important forclinical samples with low numbers of pathogen cells. One of theadvantages of RNA compared with DNA as a nucleic acid marker is itsnatural abundance in the cell. Because the gene expression valuesobtained from sequencing are relative values, the next step was toquantify the absolute copies per cell for the candidate markers. In thequantification approach, clinical isolate samples were plated after 15min of ciprofloxacin exposure to obtain cell numbers in colony formingunits (CFU/mL). Primers were designed for the candidate markers (seeExample 6 and FIG. 7 ) and measured their absolute concentration usingdPCR. The concentrations were converted to per cell values using thecell counts from plating (FIG. 3B).

Additionally, the RNA sequencing data was used to obtaintranscriptome-wide estimates of transcript copies per cell. In thesequencing approach, external RNA control consortium (ERCC) spike-inswas added to the lysis buffer step of the extraction protocol in orderto capture any loss of RNA throughout the extraction steps. By linearregression the relationship between ERCC copies added to the samples andERCC quantified by sequencing was captured. Using the linear regression,gene expression values were converted from RNA sequencing (in TPM) toapproximate copy numbers per cell (see Example 5). The transcript copiesper cell estimated for the candidate markers using the sequencingapproach were within the same order of magnitude as the absolute copiesper cell measured by digital PCR (FIG. 3B).

It is noted that gyrA and parC, which are known genotypic markers forresistance to ciprofloxacin, were not found to be differentiallyexpressed. recA, which is one of the prominent genes in the SOSresponse, was also not found to have an increased transcript levelbecause N. gonorrhoeae does not have a true SOS system[31, 32]. WhereasrecA is a specific cellular response to overcome DNA damage, the globaldownregulation that was observed suggests a general shift away fromgrowth and cell proliferation

Example 9: Validation of Markers by dPCR

To determine how the relative changes observed through RNA-seq compareto direct gene expression measurements by dPCR, dPCR assays weredesigned for candidate markers, which involved measuring the expressionof the candidate marker in both control and treated samples, andcalculating the C:T ratio.

In this assay, the 16S rRNA was also measured and used to normalize theC:T ratio of the candidate markers. In the three susceptible isolatesthat were sequenced we found that rRNA consistently showed the smallestfold change (< 1.06) in response to ciprofloxacin compared with allother genes in N. gonorrhoeae. Therefore, to account for experimentalvariations in the antibiotic exposure and RNA extraction steps betweencontrol and treated samples, the 16S rRNA was used as an intracellularcontrol for normalizing the C:T ratios (see Example 6). It was foundthat the C:T ratios measured by the dPCR assay agreed with the C:Tratios obtained through sequencing (FIG. 4 ), confirming that bothapproaches accurately capture the transcriptional response to antibioticexposure.

Example 10: Validation of RNA Markers porB and rpmB Across CDC Isolates

To determine whether candidate markers respond consistently across alarge pool of isolates with genetic variability, the two candidatemarkers with the highest abundance and fold change (porB and rpmB) werechosen to determine the susceptibility of 49 clinical isolates, with awide range of MIC values (see FIG. 8 ), from the N. gonorrhoeae panel ofthe Centers for Disease Control (CDC) Antimicrobial Resistance IsolateBank.

The MIC values were representative of the population-wide distributionvalues reported by the European Committee on AntimicrobialSusceptibility Testing[34]. Each clinical isolate was exposed tociprofloxacin for 10 min and the fold change was measured in expressionof the two candidate markers between the control and treated sampleusing dPCR (FIG. 5 ). The results show that both markers correctlyclassified all 49 CDC isolates, based on Clinical and LaboratoryStandards Institute (CLSI) breakpoint values, as 9 susceptible and 40resistant strains.

In particular, both markers were consistent in their ability tocorrectly determine susceptibility or resistance of all 49 clinicalisolates. porB demonstrated C:T ratios between 2.5 to 7 and rpmBdemonstrated C:T ratios between 2 and 6 after 10 min of antibioticexposure in the nine susceptible clinical isolates. The large foldchanges highlight the significance of using RNA response as an ASTmarker compared with quantification of DNA replication. The previouswork using dPCR quantification of DNA replication demonstrated C:Tratios between 1.2 and 2.4 for 15 min of antibiotic exposure insusceptible E. coli[30], which has a doubling time approximately 3 timesshorter than N. gonorrhoeae.

An alignment search of porB was performed against other prokaryotes andporB was found to be specific to the Neisseria genus. AST markers shouldbe specific to the pathogen of interest because additional bacterialspecies are likely to be present in clinical samples.

Example 11: DNA Quantification of N. Gonorrhoeae

Antibiotic susceptible and resistant clinical isolates plated fromglycerol stocks onto Chocolate Agar plates and grown in staticincubation overnight (37° C., 5% CO2). Cells were re-suspended in HardyFastidious Broth (HFB) and incubated for 45 min (37° C., 5% CO2) withshaking (800 rpm) to an OD600 between 1 and 5. Cultures were diluted(5X) into HFB. Each isolate culture was split into “treated” and“control” tubes. Ciprofloxacin was added to the “treated” tubes (finalconcentration of 0.5 µg/mL) and water was added to the “control” tubes;cultures were incubated (static; 37° C., 5% CO2) for 15 min. Samples forDNA quantification were extracted at 0 and 15 min using the EpicentreQuickExtract DNA Extraction Solution according to the manufacturer’sprotocol. 10 uL of sample is placed into 90 uL extraction buffer andincubated at 65° C. for 6 min, followed by 98° C. for 4 min. t0 sampleswere left at 65° C. during treatment. DNA quantification was performedby digital droplet PCR. The concentrations of the components in the dPCRmix was as follows: 1× QX200 ddPCR EvaGreen Supermix (Bio-Rad), 500 nMforward primer GTTTCAGCGGCAGCATTCA (SEQ ID NO: 176), and 500 nM reverseprimer CCGGAACTGGTTTCATCTGATT (SEQ ID NO: 177). Primers that target the16S or 23S gene of N. gonorrhoeae can be used for dPCR amplification.

Example 12: porB Sequences in 50 Clinical Isolates From the CDC Bank

In order to understand the variability of the porB gene among the 50 CDCclinical isolates, a clustal omega alignment was performed to determinethe smallest percent identity between the FA 1090 sequence and the 50CDC sequences. The percent identity was shown to be 94.94%. porB isknown to be more variable than rpmB and therefore it is likely thatpercent identity will be higher for rpmB. The porB sequences for the 50clinical isolates from the CDC bank are listed in ANNEX D (SEQ ID NO:178-227).

Example 13: Determination of Antibiotic MIC in Targeted Microorganism

An antibiotic MIC in a targeted organism can be determined in connectionwith any one of the methods herein described.

For example, when determining ciprofloxacin MIC in Neisseriagonorrhoeae, in some embodiments samples would be treated at 0.015,0.030, 0.060, 0.125, 0.25, 0.5, 1.0, 2.0, and 4.0 microgram/mL. The C:Tratios measured at each concentration would then be used to determinethe sample’s MIC. MIC could be determined, for example, by fitting acurve to the C:T ratios obtained at each concentration of antibioticplotted vs the concentration of antibiotic used for treatment, anddetermining the concentration at which the maximum slope of the curveoccurs.

This concentration of antibiotic would then correlate to a particularMIC, determined from performing this method on samples with known MICs.MIC could also be determined by the value at which the fit curve crossesa pre-defined threshold or from the lowest antibiotic concentration thatgives a CT response larger than a pre-defined threshold. MIC could alsobe determined from matching the shape of single curve (or multiplecurves) fit to the CT ratios to a pre-constructed library of curvesdetermined by performing the method on isolates with known MICs. Anexemplary curve fitting antibiotic concentrations and C:T ratios isreported in FIG. 9 .

Example 14: Determination of Type of Degree of Antibiotic Susceptibilityin Targeted Microorganism

In order to determine if a sample contains bacteria with intermediatesusceptibility, susceptible bacteria, or resistant bacteria to theantibiotic of interest, the sample can be exposed to threeconcentrations of antibiotic: a concentration equal to the susceptibleMIC breakpoint, a concentration equal to the concentration of theresistant MIC breakpoint, and a concentration equal to the average ofthe maximum and minimum of the intermediate MIC breakpoint range.Susceptibility would then be determined , for example, by measuring theslope obtained by fitting a curve or line to the three points on the C:Tratio vs treatment concentration plot, and/or by comparing the relativedifference in C:T ratio between the low and intermediate concentrationof antibiotic and the difference in CT ratio between the intermediateand high concentration, and/or by comparing the magnitude of the valuerelative to a pre-defined threshold, or a combination of these analyses.For example, for exposure or treatment of Neisseria gonorrhoeae tociprofloxacin the sample would be exposed to 0.06, 0.25, and 1.0 ug/mLciprofloxacin.

Example 15: Detection of Antibiotic Susceptibility of a N GonorrhoeaeUsing an N. Gonorrhoeae RNA Marker of the Disclosure (Prophetic)

This example follows the procedure used in [30] Schoepp, N.G., et al.,Rapid pathogen-specific phenotypic antibiotic susceptibility testingusing digital LAMP quantification in clinical samples. Sci Transl Med,2017. 9(410)). Urine containing or suspected of containing Neisseriagonorrhoeae is obtained from a patient. Urine is then mixed andincubated in exposure media with and without antibiotics. Afterincubation in exposure media, nucleic acids are extracted and the targetNeisseria gonorrhoeae RNA marker is quantified using digitalloop-mediated isothermal amplification (dLAMP). The marker concentrationin the control sample (sample without antibiotics) is divided by theconcentration in the treated sample (sample with antibiotics) togenerate a control-treated ratio (C:T ratio).

If the C:T ratio is above the threshold, Neisseria gonorrhoeae bacteriafrom this patient sample are called susceptible. If the C:T ratio isbelow the threshold, Neisseria gonorrhoeae bacteria from this patientsample are called resistant. If the C:T ratio is at the threshold, orwithin 0.05 of the threshold, Neisseria gonorrhoeae bacteria from thispatient sample are called indeterminate.

Example 16: An Exemplary Performance Standard for AntimicrobialSusceptibility Testing According to CISI Standard

The following description is taken from Clinical Laboratory StandardsInstitute (CISI) as an example for performing an AntibioticSusceptibility Test (AST) as well as breakpoint MIC values for variousbacteria according to the CLSI standard. More detailed description andupdates for CLSI documents can be further found athttps://clsi.org/standards-development/documentcorrection-notices/ aswill be understood by a person skilled in the art.

TABLE 8 below shows an exemplary zone diameter and MIC breakpoints forNeisseria gonorrhoease. Table 8: An exemplary zone diameter and MICbreakpoints for Neiseeria gonorrhoeae Testing Conditions Routine QCRecommendations (see Tables 4B and 5C of the CLSI document athttps://Clsi.org/standards/products/mi crobiology/documents/m100/foracceptable QC ranges) N. gonorrhoeae ATCCⓇ* 49226 When a commercial testsystem is used for susceptibility testing, refer to the manufacturer’sinstructions for Medium Disk diffusion: GC agar base and 1% definedgrowth supplement. (The use of a cysteine-free growth supplement is notrequired for disk diffusion testing.) Agar dilution: GC agar base and 1%defined growth supplement. (The use of a cysteine-free growth supplementis required for agar dilution tests with carbapenems and clavulanate.Cysteine-containing defined growth supplement does not significantlyalter dilution test results with other drugs.) QC test recommendationsand QC ranges. Inoculum Colony suspension, equivalent to a 0.5 McFarlandstandard prepared in MHB or 0.9% phosphate-buffered saline, pH 7, usingcolonies from an overnight (20- to 24-hour) chocolate agar plateincubated in 5% CO2 Incubation 36° C. ± 1° C. (do not exceed 37° C.); 5%CO2; all methods, 20-24 hours * ATCC® is a registered trademark of theAmerican Type Culture Collection

General Comments include:

-   (1) For disk diffusion, test a maximum of 9 disks on a 150-mm plate    and 4 disks on a 100-mm plate. For some agents, eg, fluoroquinolones    or cephalosporins, only 2 to 3 disks may be tested per plate.    Measure the diameter of the zones of complete inhibition (as judged    by the unaided eye), including the diameter of the disk. Hold the    Petri plate a few inches above a black background illuminated with    reflected light. The zone margin should be considered the area    showing no obvious, visible growth that can be detected with the    unaided eye. Ignore faint growth of tiny colonies that can be    detected only with a magnifying lens at the edge of the zone of    inhibited growth.-   (2) The clinical effectiveness of cefmetazole, cefotetan, cefoxitin,    and spectinomycin for treating infections due to organisms that    produce intermediate results with these agents is unknown.-   (3) For disk diffusion testing of N. gonorrhoeae, an intermediate    result for an antimicrobial agent indicates either a technical    problem that should be resolved by repeat testing or a lack of    clinical experience in treating infections due to organisms with    these zones. Strains with intermediate zones to agents other than    cefmetazole, cefotetan, cefoxitin, and spectinomycin have a    documented lower clinical cure rate (85% to 95%) compared with > 95%    for susceptible strains.-   (4) The recommended medium for testing N. gonorrhoeae consists of GC    agar to which a 1% defined growth supplement (1.1 g L-cystine, 0.03    g guanine HCl, 0.003 g thiamine HCl, 0.013 g para-aminobenzoic acid,    0.01 g B12, 0.1 g cocarboxylase, 0.25 g NAD, 1 g adenine, 10 g    L-glutamine, 100 g glucose, 0.02 g ferric nitrate, 25.9 g L-cysteine    HCl [in 1L H2O]) is added after autoclaving.

TABLE 9 Table 9: A list of exemplary antibiotics and their zone diameterand MIC breakpoints Test/Repo rt Group Antimicrobia 1 Agent Disk Content Interpretive Categories and Zone Diameter Breakpoints, nearest wholemm Interpretive Categories and MIC Breakpoints, µg/mL Comments S I R S IR PENICILLINS O Penicillin 10 units ≥ 47 27-46 ≤ 26 ≤ 0.06 0.12-1 ≥2 Seegeneral comment (3). (5) A positive β-lactamase test predicts resistanceto penicillin, ampicillin, and amoxicillin. (6) A β-lactamase testdetects one form of penicillin resistance in N. gonorrhoeae and also maybe used to provide epidemiological information. Strains withchromosomally mediated resistance can be detected only by the diskdiffusion method or the agar dilution MIC method. (7) Gonococci thatproduce zones of inhibition of ≤ 19 mm around a 10-unit penicillin diskare likely to be β-lactamase-producing strains. However, the β-lactamasetest remains preferable to other susceptibility methods for rapid,accurate recognition of this plasmid-mediated penicillin resistance.CEPHEMS (PARENTERAL) (Including cephalosporins I, II, III, and IV.Please refer to Glossary I.) A Ceftriaxone 30 µg ≥ 35 - - ≤ 0.25 - - OCefoxitin 30 µg ≥ 28 24-27 ≤ 23 ≤2 4 ≥8 See general comment (2) OCefuroxime 30 µg ≥31 26-30 ≤ 25 ≤1 2 ≥4 See general comment (2) OCefepime 30 µg ≥31 - - ≤ 0.5 - - O Cefmetazole 30 µg ≥ 33 28-32 ≤ 27 ≤24 ≥8 See general comment (2) O Cefotaxime 30 µg ≥31 - - ≤ 0.5 - - OCefotetan 30 µg ≥26 20-25 ≤19 ≤2 4 ≥8 See general comment (2) OCeftazidime 30 µg ≥31 - - ≤ 0.5 - - O Ceftizoxime 30 µg ≥38 - - ≤0.5 - - A Cefixime 5 µg ≥31 - - ≤ 0.25 - - O Cefpodoxime 10 µg ≥29 - ≤0.5 - - Inv. Cefetamet 10 µg ≥31 - - ≤ 0.5 - - A Tetracycline 30 µg ≥3831-37 ≤ 30 ≤ 0.25 0.5-1 ≥2 (9) Gonococci with 30-µg tetracycline diskzone diameters of ≤ 19 mm usually indicate a plasmid-mediatedtetracycline-resistant N. gonorrhoeae isolate. Resistance in thesestrains should be confirmed by a dilution test (MIC ≥16 µg/mL). ACiprofloxacin 5 µg ≥41 28-40 ≤ 27 ≤ 0.06 0.12-0.5 ≥1 O Enoxacin 10 µg≥36 32-35 ≤ 31 ≤ 0.5 1 ≥2 O Lomefloxacin 10 µg ≥38 27-37 ≤ 26 ≤ 0.120.25-1 ≥2 O Ofloxacin 5 µg ≥31 25-30 ≤ 24 ≤ 0.25 0.5-1 ≥2 Inv.Fleroxacin 5 µg ≥35 29-34 ≤ 28 ≤ 0.25 0.5 ≥1 O Spectinomycin 100 µg ≥1815-17 ≤14 ≤ 32 64 ≥ 128 See general comment (2) Abbreviations: ATCC®,American Type Culture Collection; I, intermediate; MHB, Mueller-Hintonbroth; MIC, minimal inhibitory concentration; QC, quality control; NAD,nicotinamide adenine dinucleotide; R, resistant; S, susceptible.

The examples set forth above are provided to give those of ordinaryskill in the art a complete disclosure and description of how to makeand use the embodiments of the materials, compositions, systems andmethods of the disclosure, and are not intended to limit the scope ofwhat the inventors regard as their disclosure. Those skilled in the artwill recognize how to adapt the features of the exemplified methods andsystems based on the RNA markers identified herein for detection ofsusceptibility and resistance against various antibiotics inantimicrobial-resistance bacteria according to various embodiments andscope of the claims.

All patents and publications mentioned in the specification areindicative of the levels of skill of those skilled in the art to whichthe disclosure pertains.

The entire disclosure of each document cited (including webpagespatents, patent applications, journal articles, abstracts, laboratorymanuals, books, or other disclosures) in the Background, Summary,Detailed Description, and Examples is hereby incorporated herein byreference. All references cited in this disclosure, including referencescited in any one of the Appendices, are incorporated by reference to thesame extent as if each reference had been incorporated by reference inits entirety individually. However, if any inconsistency arises betweena cited reference and the present disclosure, the present disclosuretakes precedence. Furthermore, the computer readable form of thesequence listing of the ASCII text file named“P2255-US-2021-08-23-Sq-List-ST25”, created on Aug. 23, 2021, and havinga file size (not “size on disk”) of 425 kilobytes measured on WindowsServer 2016 Standard ver. 1607, is incorporated herein by reference inits entirety.

The terms and expressions which have been employed herein are used asterms of description and not of limitation, and there is no intention inthe use of such terms and expressions of excluding any equivalents ofthe features shown and described or portions thereof, but it isrecognized that various modifications are possible within the scope ofthe disclosure claimed. Thus, it should be understood that although thedisclosure has been specifically disclosed by embodiments, exemplaryembodiments and optional features, modification and variation of theconcepts herein disclosed can be resorted to by those skilled in theart, and that such modifications and variations are considered to bewithin the scope of this disclosure as defined by the appended claims.

It is also to be understood that the terminology used herein is for thepurpose of describing particular embodiments only, and is not intendedto be limiting. As used in this specification and the appended claims,the singular forms “a,” “an,” and “the” include plural referents unlessthe content clearly dictates otherwise. The term “plurality” includestwo or more referents unless the content clearly dictates otherwise.Unless defined otherwise, all technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe art to which the disclosure pertains.

When a Markush group or other grouping is used herein, all individualmembers of the group and all combinations and possible subcombinationsof the group are intended to be individually included in the disclosure.Every combination of components or materials described or exemplifiedherein can be used to practice the disclosure, unless otherwise stated.One of ordinary skill in the art will appreciate that methods, deviceelements, and materials other than those specifically exemplified may beemployed in the practice of the disclosure without resort to undueexperimentation. All art-known functional equivalents, of any suchmethods, device elements, and materials are intended to be included inthis disclosure. Whenever a range is given in the specification, forexample, a temperature range, a frequency range, a time range, or acomposition range, all intermediate ranges and all subranges, as wellas, all individual values included in the ranges given are intended tobe included in the disclosure. Any one or more individual members of arange or group disclosed herein may be excluded from a claim of thisdisclosure. The disclosure illustratively described herein suitably maybe practiced in the absence of any element or elements, limitation orlimitations which is not specifically disclosed herein.

A number of embodiments of the disclosure have been described. Thespecific embodiments provided herein are examples of useful embodimentsof the invention and it will be apparent to one skilled in the art thatthe disclosure can be carried out using a large number of variations ofthe devices, device components, methods steps set forth in the presentdescription. As will be obvious to one of skill in the art, methods anddevices useful for the present methods may include a large number ofoptional composition and processing elements and steps.

In particular, it will be understood that various modifications may bemade without departing from the spirit and scope of the presentdisclosure. Accordingly, other embodiments are within the scope of thefollowing claims.

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ANNEX A

Appendix D: List of 16S ribosomal RNA and 23S ribosomal RNA used fornormalization

Sequences for rRNA

> A9Y61_06450: 23S ribosomal RNA (1 of 4 copies)NZ_CP016017.1:1190505-1193403 - Is on the negative strand DNA (-strand): SEQ ID NO: 1

TGAAATGATAGAGTCAAGTGAATAAGTGCATCAGGCGGATGCCTTGGCGATGATAGGCGACGAAGGACGTGTAAGCCTGCGAAAAGCGCGGGGGAGCTGGCAATAAAGCAATGATCCCGCGGTGTCCGAATGGGGAAACCCACTGCATTCTGTGCAGTATCCTAAGTTGAATACATAGGCTTAGAGAAGCGAACCCGGAGAACTGAACCATCTAAGTACCCGGAGGAAAAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAACGCGGAGGAGCCTGTACGTAATAACTGTCGAGGTAGAAGAACAAGCTGGGAAGCTTGACCATAGCGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCGTACGAAAAGTAGGGCGGGACACGTGAAATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCATCATCGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAACAGAACCTGAAACCTGATGCATACAAACAGTGGGAGCGCCCTAGTGGTGTGACTGCGTACCTTTTGTATAATGGGTCAACGACTTACATTCAGTAGCGAGCTTAACCGGATAGGGGAGGCGTAGGGAAACCGAGTCTTAATAGGGCGATGAGTTGCTGGGTGTAGACCCGAAACCGAGTGATCTATCCATGGCCAGGTTGAAGGTGCCGTAACAGGTACTGGAGGACCGAACCCACGCATGTTGCAAAATGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCTAAACAAACTCGGAGATAGCTGGTTCTCCCCGAAAACTATTTAGGTAGTGCCTCGAGCAAGACACTGATGGGGGTAAAGCACTGTTATGGCTAGGGGGTTATTGCAACTTACCAACCCATGGCAAACTCAGAATACCATCAAGTGGTTCCTCGGGAGACAGACAGCGGGTGCTAACGTCCGTTGTCAAGAGGGAAACAACCCAGACCGCCGGCTAAGGTCCCAAATGATAGATTAAGTGGTAAACGAAGTGGGAAGGCACAGACAGCCAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGTCCTGCGCGGAAGATGTAACGGGGCTCAAATCTATAACCGAAGCTGCGGATGCCGGTTTACCGGCATGGTAGGGGAGCGTTCTGTAGGCTGATGAAGGTGCATTGTAAAGTGTGCTGGAGGTATCAGAAGTGCGAATGTTGACATGAGTAGCGATAAAGCGGGTGAAAAGCCCGCTCGCCGAAAGCCCAAGGTTTCCTACGCAACGTTCATCGGCGTAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAAATGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGATTCAAATGCGATGTGGGGACGGAGAAGGTTAGGTTGGCAAGCTGTTGGAATAGCTTGTTTAAGCCGGTAGGTGGAAGACTTAGGCAAATCCGGGTTTTCTTAACACCGAAGAAGTGATGACGAGTGTTTACGGACACGAAGCAACCGATACCACGCTTCCAGGAAAAGCCACTAAGCTTCAGTTTGAATCGAACCGTACCGCAAACCGACACAGGTGGGCAGGATGAGAATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGATACCGTAACTTCGGGAGAAGGTATGCCCTCTAAGGTTAAGGACTTGCTCCGTAAGCCCCGGAGGGTCGCAGAGAATAGGTGGCTGCGACTTGTTTATTAAAAACACGAGCACTCTTGCCAACACGAAAGTGGACGTATAGGGTGTAACGCCTGCCCGGTGCCGGAAGGTTAATTGAAGATGTGCAAGCATCGGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAATGGCGTAACGATGGCCACACTGTCTCCTCCCGAGACTCAGCGAAGTTGAAGTGGTTGTGAAGATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGTCACTTGTGTAGGATAGGTGGAAGGCTTGGAAGCAAAGACGCCAGTCTCTGTGGAGTCGTCCTTGAAAATACCACCCTGGTGTCTTTGAGGTTCTAACCCAGACCCGTCATCCGGGTCGGGGACCGTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGCGTAACGGAGGAGTTCGAAGGTTACCTAGGTCCGGTCGGAAATCGGACTGATAGTGCAATGGCAAAAGGTAGCTTAACTGCGAGACCGACAAGTCGGGCAGGTGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTTGATTCCGCCCAAGAGTTCATATCGACGGCGGAGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGTAGTCGGTCCCAAGGGTATGGCTGTTCGCCATTTTAAAGTGGTACGTGAGTTGGGTTTAAAACGTCGTGAGACAGTTTGGTCCCTATCTGCAGTGGGCGTTGGAAGTTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGAACCTCTGGTGTACCGGTTGTAACGCCAGTTGCATAGCCGGGTAGCTAAGTTCGGAAGAGATAAGCGCTGAAAGCATCTAAGCGCGAAACTCGCCTGAAGATGAGACTTCCCTTGCGGTTTAACCGCACTAAAGGGTCGTTCGAGACCAGGACGTTGATAGGTGGGGTGTGGAAGCGCGGTAACGCGTGAAGCTAACCCATACTAATTGCCCGTGAGGCTTGACTCT

cDNA: SEQ ID NO: 2

AGAGTCAAGCCTCACGGGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACACCCCACCTATCAACGTCCTGGTCTCGAACGACCCTTTAGTGCGGTTAAACCGCAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCGCTTAGATGCTTTCAGCGCTTATCTCTTCCGAACTTAGCTACCCGGCTATGCAACTGGCGTTACAACCGGTACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCGTCAAACTTCCAACGCCCACTGCAGATAGGGACCAAACTGTCTCACGACGTTTTAAACCCAACTCACGTACCACTTTAAAATGGCGAACAGCCATACCCTTGGGACCGACTACAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACTCCGCCGTCGATATGAACTCTTGGGCGGAATCAAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTATGTCCTGCTTTCGCACCTGCCCGACTTGTCGGTCTCGCAGTTAAGCTACCTTTTGCCATTGCACTATCAGTCCGATTTCCGACCGGACCTAGGTAACCTTCGAACTCCTCCGTTACGCTTTGGGAGGAGACCGCCCCAGTCAAACTGCCTACCATGCACGGTCCCCGACCCGGATGACGGGTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTTCAAGGACGACTCCACAGAGACTGGCGTCTTTGCTTCCAAGCCTTCCACCTATCCTACACAAGTGACTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCAGCGGGTAGATTGCATCTTCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCCGATGCTTGCACATCTTCAATTAACCTTCCGGCACCGGGCAGGCGTTACACCCTATACGTCCACTTTCGTGTTGGCAAGAGTGCTCGTGTTTTTAATAAACAAGTCGCAGCCACCTATTCTCTGCGACCCTCCGGGGCTTACGGAGCAAGTCCTTAACCTTAGAGGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAATTCTCATCCTGCCCACCTGTGTCGGTTTGCGGTACGGTTCGATTCAAACTGAAGCTTAGTGGCTTTTCCTGGAAGCGTGGTATCGGTTGCTTCGTGTCCGTAAACACTCGTCATCACTTCTTCGGTGTTAAGAAAACCCGGATTTGCCTAAGTCTTCCACCTACCGGCTTAAACAAGCTATTCCAACAGCTTGCCAACCTAACCTTCTCCGTCCCCACATCGCATTTGAATCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCATTTCTGCCTCGCCTTAGGGGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTTCGGCGAGCGGGCTTTTCACCCGCTTTATCGCTACTCATGTCAACATTCGCACTTCTGATACCTCCAGCACACTTTACAATGCACCTTCATCAGCCTACAGAACGCTCCCCTACCATGCCGGTAAACCGGCATCCGCAGCTTCGGTTATAGATTTGAGCCCCGTTACATCTTCCGCGCAGGACGACTCGACCAGTGAGCTATTACGCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGTGCCTTCCCACTTCGTTTACCACTTAATCTATCATTTGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTTGACAACGGACGTTAGCACCCGCTGTCTGTCTCCCGAGGAACCACTTGATGGTATTCTGAGTTTGCCATGGGTTGGTAAGTTGCAATAACCCCCTAGCCATAACAGTGCTTTACCCCCATCAGTGTCTTGCTCGAGGCACTACCTAAATAGTTTTCGGGGAGAACCAGCTATCTCCGAGTTTGTTTAGCCTTTCACCCCTACCCACAGCTCATCCCCGCATTTTGCAACATGCGTGGGTTCGGTCCTCCAGTACCTGTTACGGCACCTTCAACCTGGCCATGGATAGATCACTCGGTTTCGGGTCTACACCCAGCAACTCATCGCCCTATTAAGACTCGGTTTCCCTACGCCTCCCCTATCCGGTTAAGCTCGCTACTGAATGTAAGTCGTTGACCCATTATACAAAAGGTACGCAGTCACACCACTAGGGCGCTCCCACTGTTTGTATGCATCAGGTTTCAGGTTCTGTTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCGATGATGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATTTCACGTGTCCCGCCCTACTTTTCGTACGCTTAGTACCGCTGTTGAGATTTCGAATACGGGACTGTCACCCGCTATGGTCAAGCTTCCCAGCTTGTTCTTCTACCTCGACAGTTATTACGTACAGGCTCCTCCGCGTTCGCTCGCCACTACTTGCGGAATCTCGGTTGATTTCTTTTCCTCCGGGTACTTAGATGGTTCAGTTCTCCGGGTTCGCTTCTCTAAGCCTATGTATTCAACTTAGGATACTGCACAGAATGCAGTGGGTTTCCCCATTCGGACACCGCGGGATCATTGCTTTATTGCCAGCTCCCCCGCGCTTTTCGCAGGCTTACACGTCCTTCGTCGCCTATCATCGCCAAGGCATCCGCCTGATGCACTTATTCACTTGACTCTATCATTTCA

RNA: SEQ ID NO: 3

UGAAAUGAUAGAGUCAAGUGAAUAAGUGCAUCAGGCGGAUGCCUUGGCGAUGAUAGGCGACGAAGGACGUGUAAGCCUGCGAAAAGCGCGGGGGAGCUGGCAAUAAAGCAAUGAUCCCGCGGUGUCCGAAUGGGGAAACCCACUGCAUUCUGUGCAGUAUCCUAAGUUGAAUACAUAGGCUUAGAGAAGCGAACCCGGAGAACUGAACCAUCUAAGUACCCGGAGGAAAAGAAAUCAACCGAGAUUCCGCAAGUAGUGGCGAGCGAACGCGGAGGAGCCUGUACGUAAUAACUGUCGAGGUAGAAGAACAAGCUGGGAAGCUUGACCAUAGCGGGUGACAGUCCCGUAUUCGAAAUCUCAACAGCGGUACUAAGCGUACGAAAAGUAGGGCGGGACACGUGAAAUCCUGUCUGAAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCAUCAUCGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGUGAAACAGAACCUGAAACCUGAUGCAUACAAACAGUGGGAGCGCCCUAGUGGUGUGACUGCGUACCUUUUGUAUAAUGGGUCAACGACUUACAUUCAGUAGCGAGCUUAACCGGAUAGGGGAGGCGUAGGGAAACCGAGUCUUAAUAGGGCGAUGAGUUGCUGGGUGUAGACCCGAAACCGAGUGAUCUAUCCAUGGCCAGGUUGAAGGUGCCGUAACAGGUACUGGAGGACCGAACCCACGCAUGUUGCAAAAUGCGGGGAUGAGCUGUGGGUAGGGGUGAAAGGCUAAACAAACUCGGAGAUAGCUGGUUCUCCCCGAAAACUAUUUAGGUAGUGCCUCGAGCAAGACACUGAUGGGGGUAAAGCACUGUUAUGGCUAGGGGGUUAUUGCAACUUACCAACCCAUGGCAAACUCAGAAUACCAUCAAGUGGUUCCUCGGGAGACAGACAGCGGGUGCUAACGUCCGUUGUCAAGAGGGAAACAACCCAGACCGCCGGCUAAGGUCCCAAAUGAUAGAUUAAGUGGUAAACGAAGUGGGAAGGCACAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGUCCUGCGCGGAAGAUGUAACGGGGCUCAAAUCUAUAACCGAAGCUGCGGAUGCCGGUUUACCGGCAUGGUAGGGGAGCGUUCUGUAGGCUGAUGAAGGUGCAUUGUAAAGUGUGCUGGAGGUAUCAGAAGUGCGAAUGUUGACAUGAGUAGCGAUAAAGCGGGUGAAAAGCCCGCUCGCCGAAAGCCCAAGGUUUCCUACGCAACGUUCAUCGGCGUAGGGUGAGUCGGCCCCUAAGGCGAGGCAGAAAUGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGAUUCAAAUGCGAUGUGGGGACGGAGAAGGUUAGGUUGGCAAGCUGUUGGAAUAGCUUGUUUAAGCCGGUAGGUGGAAGACUUAGGCAAAUCCGGGUUUUCUUAACACCGAAGAAGUGAUGACGAGUGUUUACGGACACGAAGCAACCGAUACCACGCUUCCAGGAAAAGCCACUAAGCUUCAGUUUGAAUCGAACCGUACCGCAAACCGACACAGGUGGGCAGGAUGAGAAUUCUAAGGCGCUUGAGAGAACUCGGGAGAAGGAACUCGGCAAAUUGAUACCGUAACUUCGGGAGAAGGUAUGCCCUCUAAGGUUAAGGACUUGCUCCGUAAGCCCCGGAGGGUCGCAGAGAAUAGGUGGCUGCGACUUGUUUAUUAAAAACACGAGCACUCUUGCCAACACGAAAGUGGACGUAUAGGGUGUAACGCCUGCCCGGUGCCGGAAGGUUAAUUGAAGAUGUGCAAGCAUCGGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAUGGCGUAACGAUGGCCACACUGUCUCCUCCCGAGACUCAGCGAAGUUGAAGUGGUUGUGAAGAUGCAAUCUACCCGCUGCUAGACGGAAAGACCCCGUGAACCUUUACUGUAGCUUUGCAUUGGACUUUGAAGUCACUUGUGUAGGAUAGGUGGAAGGCUUGGAAGCAAAGACGCCAGUCUCUGUGGAGUCGUCCUUGAAAAUACCACCCUGGUGUCUUUGAGGUUCUAACCCAGACCCGUCAUCCGGGUCGGGGACCGUGCAUGGUAGGCAGUUUGACUGGGGCGGUCUCCUCCCAAAGCGUAACGGAGGAGUUCGAAGGUUACCUAGGUCCGGUCGGAAAUCGGACUGAUAGUGCAAUGGCAAAAGGUAGCUUAACUGCGAGACCGACAAGUCGGGCAGGUGCGAAAGCAGGACAUAGUGAUCCGGUGGUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUUGAUUCCGCCCAAGAGUUCAUAUCGACGGCGGAGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGUAGUCGGUCCCAAGGGUAUGGCUGUUCGCCAUUUUAAAGUGGUACGUGAGUUGGGUUUAAAACGUCGUGAGACAGUUUGGUCCCUAUCUGCAGUGGGCGUUGGAAGUUUGACGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGAACCUCUGGUGUACCGGUUGUAACGCCAGUUGCAUAGCCGGGUAGCUAAGUUCGGAAGAGAUAAGCGCUGAAAGCAUCUAAGCGCGAAACUCGCCUGAAGAUGAGACUUCCCUUGCGGUUUAACCGCACUAAAGGGUCGUUCGAGACCAGGACGUUGAUAGGUGGGGUGUGGAAGCGCGGUAACGCGUGAAGCUAACCCAUACUAAUUGCCCGUGAGGCUU GACUCU

> A9Y61_06465: 16S ribosomal RNA (1 of 4 copies)NZ_CP016017.1:1194001-1195552 - Is on the negative strand DNA (-strand): SEQ ID NO: 4

TGAACATAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCAGCACAGGGAAGCTTGCTTCTCGGGTGGCGAGTGGCGAACGGGTGAGTAACATATCGGAACGTACCGGGTAGCGGGGGATAACTGATCGAAAGATCAGCTAATACCGCATACGTCTTGAGAGGGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCCGAGCGGCCGATATCTGATTAGCTGGTTGGCGGGGTAAAGGCCCACCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTCTGAAGAAGGCCTTCGGGTTGTAAAGGACTTTTGTCAGGGAAGAAAAGGCCGTTGCCAATATCGGCGGCCGATGACGGTACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTTAAGCAGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCTGAACTGGGTGACTCGAGTGTGTCAGAGGGAGGTGGAATTCCACGTGTAGCAGTGAAATGCGTAGAGATGTGGAGGAATACCGATGGCGAAGGCAGCCTCCTGGGATAACACTGACGTTCATGTCCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAATTAGCTGTTGGGCAACTTGATTGCTTGGTAGCGTAGCTAACGCGTGAAATTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAGAACCTTACCTGGTTTTGACATGTGCGGAATCCTCCGGAGACGGAGGAGTGCCTTCGGGAGCCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCATTCGGTTGGGCACTCTAATGAGACTGCCGGTGACAAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGACCAGGGCTTCACACGTCATACAATGGTCGGTACAGAGGGTAGCCAAGCCGCGAGGCGGAGCCAATCTCACAAAACCGATCGTAGTCCGGATTGCACTCTGCAACTCGAGTGCATGAAGTCGGAATCGCTAGTAATCGCAGGTCAGCATACTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAGTCCGCTTACCACGGTATGCTTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCAC CTCCTTTCTA

cDNA: SEQ ID NO: 5

TAGAAAGGAGGTGATCCAGCCGCAGGTTCCCCTACGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCGGACTCCTTGCGGTTACCCTACCTACTTCTGGTATCCCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCGACTTCATGCACTCGAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTGTGAGATTGGCTCCGCCTCGCGGCTTGGCTACCCTCTGTACCGACCATTGTATGACGTGTGAAGCCCTGGTCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGCTTGTCACCGGCAGTCTCATTAGAGTGCCCAACCGAATGATGGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTACGGCTCCCGAAGGCACTCCTCCGTCTCCGGAGGATTCCGCACATGTCAAAACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCACATCATCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACGCTACCAAGCAATCAAGTTGCCCAACAGCTAATTGACATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGACATGAACGTCAGTGTTATCCCAGGAGGCTGCCTTCGCCATCGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGAATTCCACCTCCCTCTGACACACTCGAGTCACCCAGTTCAGAACGCAGTTCCCGGGTTGAGCCCGGGGATTTCACATCCTGCTTAAGTAACCGTCTGCGCCCGCTTTACGCCCAGTAATTCCGATTAACGCTCGCACCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACCGTCATCGGCCGCCGATATTGGCAACGGCCTTTTCTTCCCTGACAAAAGTCCTTTACAACCCGAAGGCCTTCTTCAGACACGCGGCATGGCTGGATCAGGCTTGCGCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCTACTGATCGTCGCCTTGGTGGGCCTTTACCCCGCCAACCAGCTAATCAGATATCGGCCGCTCGGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCCCTCTCAAGACGTATGCGGTATTAGCTGATCTTTCGATCAGTTATCCCCCGCTACCCGGTACGTTCCGATATGTTACTCACCCGTTCGCCACTCGCCACCCGAGAAGCAAGCTTCCCTGTGCTGCCGTCCGACTTGCATGTGTAAAGCATGCCGCCAGCGTTCAATCTGAGCCAGGATCAAACTCTTATGTTCA

RNA: SEQ ID NO: 6

UGAACAUAAGAGUUUGAUCCUGGCUCAGAUUGAACGCUGGCGGCAUGCUUUACACAUGCAAGUCGGACGGCAGCACAGGGAAGCUUGCUUCUCGGGUGGCGAGUGGCGAACGGGUGAGUAACAUAUCGGAACGUACCGGGUAGCGGGGGAUAACUGAUCGAAAGAUCAGCUAAUACCGCAUACGUCUUGAGAGGGAAAGCAGGGGACCUUCGGGCCUUGCGCUAUCCGAGCGGCCGAUAUCUGAUUAGCUGGUUGGCGGGGUAAAGGCCCACCAAGGCGACGAUCAGUAGCGGGUCUGAGAGGAUGAUCCGCCACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUUUUGGACAAUGGGCGCAAGCCUGAUCCAGCCAUGCCGCGUGUCUGAAGAAGGCCUUCGGGUUGUAAAGGACUUUUGUCAGGGAAGAAAAGGCCGUUGCCAAUAUCGGCGGCCGAUGACGGUACCUGAAGAAUAAGCACCGGCUAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGGUGCGAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGGGCGCAGACGGUUACUUAAGCAGGAUGUGAAAUCCCCGGGCUCAACCCGGGAACUGCGUUCUGAACUGGGUGACUCGAGUGUGUCAGAGGGAGGUGGAAUUCCACGUGUAGCAGUGAAAUGCGUAGAGAUGUGGAGGAAUACCGAUGGCGAAGGCAGCCUCCUGGGAUAACACUGACGUUCAUGUCCGAAAGCGUGGGUAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCCUAAACGAUGUCAAUUAGCUGUUGGGCAACUUGAUUGCUUGGUAGCGUAGCUAACGCGUGAAAUUGACCGCCUGGGGAGUACGGUCGCAAGAUUAAAACUCAAAGGAAUUGACGGGGACCCGCACAAGCGGUGGAUGAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUUUUGACAUGUGCGGAAUCCUCCGGAGACGGAGGAGUGCCUUCGGGAGCCGUAACACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUGUCAUUAGUUGCCAUCAUUCGGUUGGGCACUCUAAUGAGACUGCCGGUGACAAGCCGGAGGAAGGUGGGGAUGACGUCAAGUCCUCAUGGCCCUUAUGACCAGGGCUUCACACGUCAUACAAUGGUCGGUACAGAGGGUAGCCAAGCCGCGAGGCGGAGCCAAUCUCACAAAACCGAUCGUAGUCCGGAUUGCACUCUGCAACUCGAGUGCAUGAAGUCGGAAUCGCUAGUAAUCGCAGGUCAGCAUACUGCGGUGAAUACGUUCCCGGGUCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGGGAUACCAGAAGUAGGUAGGGUAACCGCAAGGAGUCCGCUUACCACGGUAUGCUUCAUGACUGGGGUGAAGUCGUAACAAGGUAGCCGUAGGGGAACCUGCGGCUGGAUCACCUCCUUUCU A

> A9Y61_07175: 23S ribosomal RNA (1 of 4 copies)NZ_CP016017.1:1325810-1328708 - Is on the negative strand DNA (-strand): SEQ ID NO: 7

TGAAATGATAGAGTCAAGTGAATAAGTGCATCAGGCGGATGCCTTGGCGATGATAGGCGACGAAGGACGTGTAAGCCTGCGAAAAGCGCGGGGGAGCTGGCAATAAAGCAATGATCCCGCGGTGTCCGAATGGGGAAACCCACTGCATTCTGTGCAGTATCCTAAGTTGAATACATAGGCTTAGAGAAGCGAACCCGGAGAACTGAACCATCTAAGTACCCGGAGGAAAAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAACGCGGAGGAGCCTGTACGTAATAACTGTCGAGGTAGAAGAACAAGCTGGGAAGCTTGACCATAGCGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCGTACGAAAAGTAGGGCGGGACACGTGAAATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCATCATCGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAACAGAACCTGAAACCTGATGCATACAAACAGTGGGAGCGCCCTAGTGGTGTGACTGCGTACCTTTTGTATAATGGGTCAACGACTTACATTCAGTAGCGAGCTTAACCGGATAGGGGAGGCGTAGGGAAACCGAGTCTTAATAGGGCGATGAGTTGCTGGGTGTAGACCCGAAACCGAGTGATCTATCCATGGCCAGGTTGAAGGTGCCGTAACAGGTACTGGAGGACCGAACCCACGCATGTTGCAAAATGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCTAAACAAACTCGGAGATAGCTGGTTCTCCCCGAAAACTATTTAGGTAGTGCCTCGAGCAAGACACTGATGGGGGTAAAGCACTGTTATGGCTAGGGGGTTATTGCAACTTACCAACCCATGGCAAACTCAGAATACCATCAAGTGGTTCCTCGGGAGACAGACAGCGGGTGCTAACGTCCGTTGTCAAGAGGGAAACAACCCAGACCGCCGGCTAAGGTCCCAAATGATAGATTAAGTGGTAAACGAAGTGGGAAGGCACAGACAGCCAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGTCCTGCGCGGAAGATGTAACGGGGCTCAAATCTATAACCCAAGCTGCGTATGCCGGTTTACCGGCATGGTAGGGGAGCGTTCTGTAGGCTGATGAAGGTGCATTGTAAAGTGTGCTGGAGGTATCAGAAGTGCGAATGTTGACATGAGTAGCGATAAAGCGGGTGAAAAGCCCGCTCGCCGCAAAGCCCAAGGTTTCCTACGCAACGTTCATCGGCGTAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAAATGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGATTCAAATGCGATGTGGGGACGGAGAAGGTTAGGTTGGCAAGCTGTTGGAATAGCTTGTTTAAGCCGGTAGGTGGAAGACTTAGGCAAATCCGGGTTTTCTTAACACCGAGAAGTGATGACGAGTGTCTACGGACACGAAGCAACCGATACCACGCTTCCAGGAAAAGCCACTAAGCTTCAGTTTGAATCGAACCGTACCGCAAACCGACACAGGTGGGCAGGATGAGAATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGATACCGTAACTTCGGGAGAAGGTATGCCCTCTAAGGTTAAGGACTTGCTCCGTAAGCCCCGGAGGGTCGCAGAGAATAGGTGGCTGCGACTGTTTATTAAAAACACAGCACTCTGCCAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGAAGATGTGCAAGCATCGGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAATGGCGTAACGATGGCCACACTGTCTCCTCCCGAGACTCAGCGAAGTTGAAGTGGTTGTGAAGATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGTCACTTGTGTAGGATAGGTGGGAGGCTTGGAAGCAGAGACGCCAGTCTCTGTGGAGTCGTCCTTGAAATACCACCCTGGTGTCTTTGAGGTTCTAACCCAGACCCGTCATCCGGGTCGGGGACCGTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGCGTAACGGAGGAGTTCGAAGGTTACCTAGGTCCGGTCGGAAATCGGACTGATAGTGCAATGGCAAAAGGTAGCTTAACTGCGAGACCGACAAGTCGGGCAGGTGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATTCCGCCCAAGAGTTCATATCGACGGCGGAGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGTAGTCGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGTGAGCTGGGTTTAAAACGTCGTGAGACAGTTTGGTCCCTATCTGCAGTGGGCGTTGGAAGTTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGAACCTCTGGTGTACCGGTTGTAACGCCAGTTGCATAGCCGGGTAGCTAAGTTCGGAAGAGATAAGCGCTGAAAGCATCTAAGCGCGAAACTCGCCTGAAGATGAGACTTCCCTTGCGGTTTAACCGCACTAAAGGGTCGTTCGAGACCAGGACGTTGATAGGTGGGGTGTGGAAGCGCGGTAACGCGTGAAGCTAACCCATACTAATTGCCCGTGAGGCTTGACTCT

cDNA: SEQ ID NO: 8

AGAGTCAAGCCTCACGGGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACACCCCACCTATCAACGTCCTGGTCTCGAACGACCCTTTAGTGCGGTTAAACCGCAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCGCTTAGATGCTTTCAGCGCTTATCTCTTCCGAACTTAGCTACCCGGCTATGCAACTGGCGTTACAACCGGTACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCGTCAAACTTCCAACGCCCACTGCAGATAGGGACCAAACTGTCTCACGACGTTTTAAACCCAGCTCACGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTACAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACTCCGCCGTCGATATGAACTCTTGGGCGGAATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTATGTCCTGCTTTCGCACCTGCCCGACTTGTCGGTCTCGCAGTTAAGCTACCTTTTGCCATTGCACTATCAGTCCGATTTCCGACCGGACCTAGGTAACCTTCGAACTCCTCCGTTACGCTTTGGGAGGAGACCGCCCCAGTCAAACTGCCTACCATGCACGGTCCCCGACCCGGATGACGGGTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGACTCCACAGAGACTGGCGTCTCTGCTTCCAAGCCTCCCACCTATCCTACACAAGTGACTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCAGCGGGTAGATTGCATCTTCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCCGATGCTTGCACATCTTCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTGGCAGAGTGCTGTGTTTTTAATAAACAGTCGCAGCCACCTATTCTCTGCGACCCTCCGGGGCTTACGGAGCAAGTCCTTAACCTTAGAGGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAATTCTCATCCTGCCCACCTGTGTCGGTTTGCGGTACGGTTCGATTCAAACTGAAGCTTAGTGGCTTTTCCTGGAAGCGTGGTATCGGTTGCTTCGTGTCCGTAGACACTCGTCATCACTTCTCGGTGTTAAGAAAACCCGGATTTGCCTAAGTCTTCCACCTACCGGCTTAAACAAGCTATTCCAACAGCTTGCCAACCTAACCTTCTCCGTCCCCACATCGCATTTGAATCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCATTTCTGCCTCGCCTTAGGGGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTTGCGGCGAGCGGGCTTTTCACCCGCTTTATCGCTACTCATGTCAACATTCGCACTTCTGATACCTCCAGCACACTTTACAATGCACCTTCATCAGCCTACAGAACGCTCCCCTACCATGCCGGTAAACCGGCATACGCAGCTTGGGTTATAGATTTGAGCCCCGTTACATCTTCCGCGCAGGACGACTCGACCAGTGAGCTATTACGCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGTGCCTTCCCACTTCGTTTACCACTTAATCTATCATTTGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTTGACAACGGACGTTAGCACCCGCTGTCTGTCTCCCGAGGAACCACTTGATGGTATTCTGAGTTTGCCATGGGTTGGTAAGTTGCAATAACCCCCTAGCCATAACAGTGCTTTACCCCCATCAGTGTCTTGCTCGAGGCACTACCTAAATAGTTTTCGGGGAGAACCAGCTATCTCCGAGTTTGTTTAGCCTTTCACCCCTACCCACAGCTCATCCCCGCATTTTGCAACATGCGTGGGTTCGGTCCTCCAGTACCTGTTACGGCACCTTCAACCTGGCCATGGATAGATCACTCGGTTTCGGGTCTACACCCAGCAACTCATCGCCCTATTAAGACTCGGTTTCCCTACGCCTCCCCTATCCGGTTAAGCTCGCTACTGAATGTAAGTCGTTGACCCATTATACAAAAGGTACGCAGTCACACCACTAGGGCGCTCCCACTGTTTGTATGCATCAGGTTTCAGGTTCTGTTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCGATGATGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATTTCACGTGTCCCGCCCTACTTTTCGTACGCTTAGTACCGCTGTTGAGATTTCGAATACGGGACTGTCACCCGCTATGGTCAAGCTTCCCAGCTTGTTCTTCTACCTCGACAGTTATTACGTACAGGCTCCTCCGCGTTCGCTCGCCACTACTTGCGGAATCTCGGTTGATTTCTTTTCCTCCGGGTACTTAGATGGTTCAGTTCTCCGGGTTCGCTTCTCTAAGCCTATGTATTCAACTTAGGATACTGCACAGAATGCAGTGGGTTTCCCCATTCGGACACCGCGGGATCATTGCTTTATTGCCAGCTCCCCCGCGCTTTTCGCAGGCTTACACGTCCTTCGTCGCCTATCATCGCCAAGGCATCCGCCTGATGCACTTATTCACTTGACTCTATCATTTCA

RNA: SEQ ID NO: 9

UGAAAUGAUAGAGUCAAGUGAAUAAGUGCAUCAGGCGGAUGCCUUGGCGAUGAUAGGCGACGAAGGACGUGUAAGCCUGCGAAAAGCGCGGGGGAGCUGGCAAUAAAGCAAUGAUCCCGCGGUGUCCGAAUGGGGAAACCCACUGCAUUCUGUGCAGUAUCCUAAGUUGAAUACAUAGGCUUAGAGAAGCGAACCCGGAGAACUGAACCAUCUAAGUACCCGGAGGAAAAGAAAUCAACCGAGAUUCCGCAAGUAGUGGCGAGCGAACGCGGAGGAGCCUGUACGUAAUAACUGUCGAGGUAGAAGAACAAGCUGGGAAGCUUGACCAUAGCGGGUGACAGUCCCGUAUUCGAAAUCUCAACAGCGGUACUAAGCGUACGAAAAGUAGGGCGGGACACGUGAAAUCCUGUCUGAAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCAUCAUCGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGUGAAACAGAACCUGAAACCUGAUGCAUACAAACAGUGGGAGCGCCCUAGUGGUGUGACUGCGUACCUUUUGUAUAAUGGGUCAACGACUUACAUUCAGUAGCGAGCUUAACCGGAUAGGGGAGGCGUAGGGAAACCGAGUCUUAAUAGGGCGAUGAGUUGCUGGGUGUAGACCCGAAACCGAGUGAUCUAUCCAUGGCCAGGUUGAAGGUGCCGUAACAGGUACUGGAGGACCGAACCCACGCAUGUUGCAAAAUGCGGGGAUGAGCUGUGGGUAGGGGUGAAAGGCUAAACAAACUCGGAGAUAGCUGGUUCUCCCCGAAAACUAUUUAGGUAGUGCCUCGAGCAAGACACUGAUGGGGGUAAAGCACUGUUAUGGCUAGGGGGUUAUUGCAACUUACCAACCCAUGGCAAACUCAGAAUACCAUCAAGUGGUUCCUCGGGAGACAGACAGCGGGUGCUAACGUCCGUUGUCAAGAGGGAAACAACCCAGACCGCCGGCUAAGGUCCCAAAUGAUAGAUUAAGUGGUAAACGAAGUGGGAAGGCACAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGUCCUGCGCGGAAGAUGUAACGGGGCUCAAAUCUAUAACCCAAGCUGCGUAUGCCGGUUUACCGGCAUGGUAGGGGAGCGUUCUGUAGGCUGAUGAAGGUGCAUUGUAAAGUGUGCUGGAGGUAUCAGAAGUGCGAAUGUUGACAUGAGUAGCGAUAAAGCGGGUGAAAAGCCCGCUCGCCGCAAAGCCCAAGGUUUCCUACGCAACGUUCAUCGGCGUAGGGUGAGUCGGCCCCUAAGGCGAGGCAGAAAUGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGAUUCAAAUGCGAUGUGGGGACGGAGAAGGUUAGGUUGGCAAGCUGUUGGAAUAGCUUGUUUAAGCCGGUAGGUGGAAGACUUAGGCAAAUCCGGGUUUUCUUAACACCGAGAAGUGAUGACGAGUGUCUACGGACACGAAGCAACCGAUACCACGCUUCCAGGAAAAGCCACUAAGCUUCAGUUUGAAUCGAACCGUACCGCAAACCGACACAGGUGGGCAGGAUGAGAAUUCUAAGGCGCUUGAGAGAACUCGGGAGAAGGAACUCGGCAAAUUGAUACCGUAACUUCGGGAGAAGGUAUGCCCUCUAAGGUUAAGGACUUGCUCCGUAAGCCCCGGAGGGUCGCAGAGAAUAGGUGGCUGCGACUGUUUAUUAAAAACACAGCACUCUGCCAACACGAAAGUGGACGUAUAGGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAAGAUGUGCAAGCAUCGGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAUGGCGUAACGAUGGCCACACUGUCUCCUCCCGAGACUCAGCGAAGUUGAAGUGGUUGUGAAGAUGCAAUCUACCCGCUGCUAGACGGAAAGACCCCGUGAACCUUUACUGUAGCUUUGCAUUGGACUUUGAAGUCACUUGUGUAGGAUAGGUGGGAGGCUUGGAAGCAGAGACGCCAGUCUCUGUGGAGUCGUCCUUGAAAUACCACCCUGGUGUCUUUGAGGUUCUAACCCAGACCCGUCAUCCGGGUCGGGGACCGUGCAUGGUAGGCAGUUUGACUGGGGCGGUCUCCUCCCAAAGCGUAACGGAGGAGUUCGAAGGUUACCUAGGUCCGGUCGGAAAUCGGACUGAUAGUGCAAUGGCAAAAGGUAGCUUAACUGCGAGACCGACAAGUCGGGCAGGUGCGAAAGCAGGACAUAGUGAUCCGGUGGUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUUCCGCCCAAGAGUUCAUAUCGACGGCGGAGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGUAGUCGGUCCCAAGGGUAUGGCUGUUCGCCAUUUAAAGUGGUACGUGAGCUGGGUUUAAAACGUCGUGAGACAGUUUGGUCCCUAUCUGCAGUGGGCGUUGGAAGUUUGACGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGAACCUCUGGUGUACCGGUUGUAACGCCAGUUGCAUAGCCGGGUAGCUAAGUUCGGAAGAGAUAAGCGCUGAAAGCAUCUAAGCGCGAAACUCGCCUGAAGAUGAGACUUCCCUUGCGGUUUAACCGCACUAAAGGGUCGUUCGAGACCAGGACGUUGAUAGGUGGGGUGUGGAAGCGCGGUAACGCGUGAAGCUAACCCAUACUAAUUGCCCGUGAGGCUUGACUCU

> A9Y61_RS07190: 16S ribosomal RNA (1 of 4 copies)NZ_CP016017.1:1329306-1330857 - Is on the negative strand DNA (+strand): SEQ ID NO: 13

TGAACATAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCAGCACAGGGAAGCTTGCTTCTCGGGTGGCGAGTGGCGAACGGGTGAGTAACATATCGGAACGTACCGGGTAGCGGGGGATAACTGATCGAAAGATCAGCTAATACCGCATACGTCTTGAGAGGGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCCGAGCGGCCGATATCTGATTAGCTGGTTGGCGGGGTAAAGGCCCACCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTCTGAAGAAGGCCTTCGGGTTGTAAAGGACTTTTGTCAGGGAAGAAAAGGCCGTTGCCAATATCGGCGGCCGATGACGGTACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTTAAGCAGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCTGAACTGGGTGACTCGAGTGTGTCAGAGGGAGGTGGAATTCCACGTGTAGCAGTGAAATGCGTAGAGATGTGGAGGAATACCGATGGCGAAGGCAGCCTCCTGGGATAACACTGACGTTCATGTCCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAATTAGCTGTTGGGCAACTTGATTGCTTGGTAGCGTAGCTAACGCGTGAAATTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAGAACCTTACCTGGTTTTGACATGTGCGGAATCCTCCGGAGACGGAGGAGTGCCTTCGGGAGCCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCATTCGGTTGGGCACTCTAATGAGACTGCCGGTGACAAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGACCAGGGCTTCACACGTCATACAATGGTCGGTACAGAGGGTAGCCAAGCCGCGAGGCGGAGCCAATCTCACAAAACCGATCGTAGTCCGGATTGCACTCTGCAACTCGAGTGCATGAAGTCGGAATCGCTAGTAATCGCAGGTCAGCATACTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAGTCCGCTTACCACGGTATGCTTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCAC CTCCTTTCTA

cDNA: SEQ ID NO: 14

TAGAAAGGAGGTGATCCAGCCGCAGGTTCCCCTACGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCGGACTCCTTGCGGTTACCCTACCTACTTCTGGTATCCCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCGACTTCATGCACTCGAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTGTGAGATTGGCTCCGCCTCGCGGCTTGGCTACCCTCTGTACCGACCATTGTATGACGTGTGAAGCCCTGGTCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGCTTGTCACCGGCAGTCTCATTAGAGTGCCCAACCGAATGATGGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTACGGCTCCCGAAGGCACTCCTCCGTCTCCGGAGGATTCCGCACATGTCAAAACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCACATCATCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACGCTACCAAGCAATCAAGTTGCCCAACAGCTAATTGACATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGACATGAACGTCAGTGTTATCCCAGGAGGCTGCCTTCGCCATCGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGAATTCCACCTCCCTCTGACACACTCGAGTCACCCAGTTCAGAACGCAGTTCCCGGGTTGAGCCCGGGGATTTCACATCCTGCTTAAGTAACCGTCTGCGCCCGCTTTACGCCCAGTAATTCCGATTAACGCTCGCACCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACCGTCATCGGCCGCCGATATTGGCAACGGCCTTTTCTTCCCTGACAAAAGTCCTTTACAACCCGAAGGCCTTCTTCAGACACGCGGCATGGCTGGATCAGGCTTGCGCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCTACTGATCGTCGCCTTGGTGGGCCTTTACCCCGCCAACCAGCTAATCAGATATCGGCCGCTCGGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCCCTCTCAAGACGTATGCGGTATTAGCTGATCTTTCGATCAGTTATCCCCCGCTACCCGGTACGTTCCGATATGTTACTCACCCGTTCGCCACTCGCCACCCGAGAAGCAAGCTTCCCTGTGCTGCCGTCCGACTTGCATGTGTAAAGCATGCCGCCAGCGTTCAATCTGAGCCAGGATCAAACTCTTATGTTCA

RNA: SEQ ID NO: 15

UGAACAUAAGAGUUUGAUCCUGGCUCAGAUUGAACGCUGGCGGCAUGCUUUACACAUGCAAGUCGGACGGCAGCACAGGGAAGCUUGCUUCUCGGGUGGCGAGUGGCGAACGGGUGAGUAACAUAUCGGAACGUACCGGGUAGCGGGGGAUAACUGAUCGAAAGAUCAGCUAAUACCGCAUACGUCUUGAGAGGGAAAGCAGGGGACCUUCGGGCCUUGCGCUAUCCGAGCGGCCGAUAUCUGAUUAGCUGGUUGGCGGGGUAAAGGCCCACCAAGGCGACGAUCAGUAGCGGGUCUGAGAGGAUGAUCCGCCACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUUUUGGACAAUGGGCGCAAGCCUGAUCCAGCCAUGCCGCGUGUCUGAAGAAGGCCUUCGGGUUGUAAAGGACUUUUGUCAGGGAAGAAAAGGCCGUUGCCAAUAUCGGCGGCCGAUGACGGUACCUGAAGAAUAAGCACCGGCUAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGGUGCGAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGGGCGCAGACGGUUACUUAAGCAGGAUGUGAAAUCCCCGGGCUCAACCCGGGAACUGCGUUCUGAACUGGGUGACUCGAGUGUGUCAGAGGGAGGUGGAAUUCCACGUGUAGCAGUGAAAUGCGUAGAGAUGUGGAGGAAUACCGAUGGCGAAGGCAGCCUCCUGGGAUAACACUGACGUUCAUGUCCGAAAGCGUGGGUAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCCUAAACGAUGUCAAUUAGCUGUUGGGCAACUUGAUUGCUUGGUAGCGUAGCUAACGCGUGAAAUUGACCGCCUGGGGAGUACGGUCGCAAGAUUAAAACUCAAAGGAAUUGACGGGGACCCGCACAAGCGGUGGAUGAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUUUUGACAUGUGCGGAAUCCUCCGGAGACGGAGGAGUGCCUUCGGGAGCCGUAACACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUGUCAUUAGUUGCCAUCAUUCGGUUGGGCACUCUAAUGAGACUGCCGGUGACAAGCCGGAGGAAGGUGGGGAUGACGUCAAGUCCUCAUGGCCCUUAUGACCAGGGCUUCACACGUCAUACAAUGGUCGGUACAGAGGGUAGCCAAGCCGCGAGGCGGAGCCAAUCUCACAAAACCGAUCGUAGUCCGGAUUGCACUCUGCAACUCGAGUGCAUGAAGUCGGAAUCGCUAGUAAUCGCAGGUCAGCAUACUGCGGUGAAUACGUUCCCGGGUCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGGGAUACCAGAAGUAGGUAGGGUAACCGCAAGGAGUCCGCUUACCACGGUAUGCUUCAUGACUGGGGUGAAGUCGUAACAAGGUAGCCGUAGGGGAACCUGCGGCUGGAUCACCUCCUUUCU A

> A9Y61_09315: 23S ribosomal RNA (1 of 4 copies)NZ_CP016017.1:1718894-1721792 - Is on the negative strand DNA (-strand): SEQ ID NO: 16

TGAAATGATAGAGTCAAGTGAATAAGTGCATCAGGCGGATGCCTTGGCGATGATAGGCGACGAAGGACGTGTAAGCCTGCGAAAAGCGCGGGGGAGCTGGCAATAAAGCAATGATCCCGCGGTGTCCGAATGGGGAAACCCACTGCATTCTGTGCAGTATCCTAAGTTGAATACATAGGCTTAGAGAAGCGAACCCGGAGAACTGAACCATCTAAGTACCCGGAGGAAAAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAACGCGGAGGAGCCTGTACGTAATAACTGTCGAGGTAGAAGAACAAGCTGGGAAGCTTGACCATAGCGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCGTACGAAAAGTAGGGCGGGACACGTGAAATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCATCATCGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAACAGAACCTGAAACCTGATGCATACAAACAGTGGGAGCGCCCTAGTGGTGTGACTGCGTACCTTTTGTATAATGGGTCAACGACTTACATTCAGTAGCGAGCTTAACCGGATAGGGGAGGCGTAGGGAAACCGAGTCTTAATAGGGCGATGAGTTGCTGGGTGTAGACCCGAAACCGAGTGATCTATCCATGGCCAGGTTGAAGGTGCCGTAACAGGTACTGGAGGACCGAACCCACGCATGTTGCAAAATGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCTAAACAAACTCGGAGATAGCTGGTTCTCCCCGAAAACTATTTAGGTAGTGCCTCGAGCAAGACACTGATGGGGGTAAAGCACTGTTATGGCTAGGGGGTTATTGCAACTTACCAACCCATGGCAAACTCAGAATACCATCAAGTGGTTCCTCGGGAGACAGACAGCGGGTGCTAACGTCCGTTGTCAAGAGGGAAACAACCCAGACCGCCGGCTAAGGTCCCAAATGATAGATTAAGTGGTAAACGAAGTGGGAAGGCACAGACAGCCAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGTCCTGCGCGGAAGATGTAACGGGGCTCAAATCTATAACCGAAGCTGCGGATGCCGGTTTACCGGCATGGTAGGGGAGCGTTCTGTAGGCTGATGAAGGTGCATTGTAAAGTGTGCTGGAGGTATCAGAAGTGCGAATGTTGACATGAGTAGCGATAAAGCGGGTGAAAAGCCCGCTCGCCGAAAGCCCAAGGTTTCCTACGCAACGTTCATCGGCGTAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAAATGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGATTCAAATGCGATGTGGGGACGGAGAAGGTTAGGTTGGCAAGCTGTTGGAATAGCTTGTTTAAGCCGGTAGGTGGAAGACTTAGGCAAATCCGGGTTTTCTTAACACCGAGAAGTGATGACGAGTGTCTACGGACACGAAGCAACCGATACCACGCTTCCAGGAAAAGCCACTAAGCTTCAGTTTGAATCGAACCGTACCGCAAACCGACACAGGTGGGCAGGATGAGAATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGATACCGTAACTTCGGGAGAAGGTATGCCCTCTAAGGTTAAGGACTTGCTCCGTAAGCCCCGGAGGGTCGCAGAGAATAGGTGGCTGCGACTGTTTATTAAAAACACAGCACTCTGCCAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGAAGATGTGCAAGCATCGGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAATGGCGTAACGATGGCCACACTGTCTCCTCCCGAGACTCAGCGAAGTTGAAGTGGTTGTGAAGATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGTCACTTGTGTAGGATAGGTGGGAGGCTTGGAAGCAGAGACGCCAGTCTCTGTGGAGTCGTCCTTGAAATACCACCCTGGTGTCTTTGAGGTTCTAACCCAGACCCGTCATCCGGGTCGGGGACCGTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGCGTAACGGAGGAGTTCGAAGGTTACCTAGGTCCGGTCGGAAATCGGACTGATAGTGCAATGGCAAAAGGTAGCTTAACTGCGAGACCGACAAGTCGGGCAGGTGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATTCCGCCCAAGAGTTCATATCGACGGCGGAGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGTAGTCGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGTGAGCTGGGTTTAAAACGTCGTGAGACAGTTTGGTCCCTATCTGCAGTGGGCGTTGGAAGTTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGAACCTCTGGTGTACCGGTTGTAACGCCAGTTGCATAGCCGGGTAGCTAAGTTCGGAAGAGATAAGCGCTGAAAGCATCTAAGCGCGAAACTCGCCTGAAGATGAGACTTCCCTTGCGGTTTAACCGCACTAAAGGGTCGTTCGAGACCAGGACGTTGATAGGTGGGGTGTGGAAGCGCGGTAACGCGTGAAGCTAACCCATACTAATTGCCCGTGAGGCTTGACTCT

cDNA: SEQ ID NO: 17

AGAGTCAAGCCTCACGGGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACACCCCACCTATCAACGTCCTGGTCTCGAACGACCCTTTAGTGCGGTTAAACCGCAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCGCTTAGATGCTTTCAGCGCTTATCTCTTCCGAACTTAGCTACCCGGCTATGCAACTGGCGTTACAACCGGTACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCGTCAAACTTCCAACGCCCACTGCAGATAGGGACCAAACTGTCTCACGACGTTTTAAACCCAGCTCACGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTACAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACTCCGCCGTCGATATGAACTCTTGGGCGGAATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTATGTCCTGCTTTCGCACCTGCCCGACTTGTCGGTCTCGCAGTTAAGCTACCTTTTGCCATTGCACTATCAGTCCGATTTCCGACCGGACCTAGGTAACCTTCGAACTCCTCCGTTACGCTTTGGGAGGAGACCGCCCCAGTCAAACTGCCTACCATGCACGGTCCCCGACCCGGATGACGGGTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGACTCCACAGAGACTGGCGTCTCTGCTTCCAAGCCTCCCACCTATCCTACACAAGTGACTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCAGCGGGTAGATTGCATCTTCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCCGATGCTTGCACATCTTCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTGGCAGAGTGCTGTGTTTTTAATAAACAGTCGCAGCCACCTATTCTCTGCGACCCTCCGGGGCTTACGGAGCAAGTCCTTAACCTTAGAGGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAATTCTCATCCTGCCCACCTGTGTCGGTTTGCGGTACGGTTCGATTCAAACTGAAGCTTAGTGGCTTTTCCTGGAAGCGTGGTATCGGTTGCTTCGTGTCCGTAGACACTCGTCATCACTTCTCGGTGTTAAGAAAACCCGGATTTGCCTAAGTCTTCCACCTACCGGCTTAAACAAGCTATTCCAACAGCTTGCCAACCTAACCTTCTCCGTCCCCACATCGCATTTGAATCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCATTTCTGCCTCGCCTTAGGGGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTTCGGCGAGCGGGCTTTTCACCCGCTTTATCGCTACTCATGTCAACATTCGCACTTCTGATACCTCCAGCACACTTTACAATGCACCTTCATCAGCCTACAGAACGCTCCCCTACCATGCCGGTAAACCGGCATCCGCAGCTTCGGTTATAGATTTGAGCCCCGTTACATCTTCCGCGCAGGACGACTCGACCAGTGAGCTATTACGCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGTGCCTTCCCACTTCGTTTACCACTTAATCTATCATTTGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTTGACAACGGACGTTAGCACCCGCTGTCTGTCTCCCGAGGAACCACTTGATGGTATTCTGAGTTTGCCATGGGTTGGTAAGTTGCAATAACCCCCTAGCCATAACAGTGCTTTACCCCCATCAGTGTCTTGCTCGAGGCACTACCTAAATAGTTTTCGGGGAGAACCAGCTATCTCCGAGTTTGTTTAGCCTTTCACCCCTACCCACAGCTCATCCCCGCATTTTGCAACATGCGTGGGTTCGGTCCTCCAGTACCTGTTACGGCACCTTCAACCTGGCCATGGATAGATCACTCGGTTTCGGGTCTACACCCAGCAACTCATCGCCCTATTAAGACTCGGTTTCCCTACGCCTCCCCTATCCGGTTAAGCTCGCTACTGAATGTAAGTCGTTGACCCATTATACAAAAGGTACGCAGTCACACCACTAGGGCGCTCCCACTGTTTGTATGCATCAGGTTTCAGGTTCTGTTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCGATGATGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATTTCACGTGTCCCGCCCTACTTTTCGTACGCTTAGTACCGCTGTTGAGATTTCGAATACGGGACTGTCACCCGCTATGGTCAAGCTTCCCAGCTTGTTCTTCTACCTCGACAGTTATTACGTACAGGCTCCTCCGCGTTCGCTCGCCACTACTTGCGGAATCTCGGTTGATTTCTTTTCCTCCGGGTACTTAGATGGTTCAGTTCTCCGGGTTCGCTTCTCTAAGCCTATGTATTCAACTTAGGATACTGCACAGAATGCAGTGGGTTTCCCCATTCGGACACCGCGGGATCATTGCTTTATTGCCAGCTCCCCCGCGCTTTTCGCAGGCTTACACGTCCTTCGTCGCCTATCATCGCCAAGGCATCCGCCTGATGCACTTATTCACTTGACTCTATCATTTCA

RNA: SEQ ID NO: 18

UGAAAUGAUAGAGUCAAGUGAAUAAGUGCAUCAGGCGGAUGCCUUGGCGAUGAUAGGCGACGAAGGACGUGUAAGCCUGCGAAAAGCGCGGGGGAGCUGGCAAUAAAGCAAUGAUCCCGCGGUGUCCGAAUGGGGAAACCCACUGCAUUCUGUGCAGUAUCCUAAGUUGAAUACAUAGGCUUAGAGAAGCGAACCCGGAGAACUGAACCAUCUAAGUACCCGGAGGAAAAGAAAUCAACCGAGAUUCCGCAAGUAGUGGCGAGCGAACGCGGAGGAGCCUGUACGUAAUAACUGUCGAGGUAGAAGAACAAGCUGGGAAGCUUGACCAUAGCGGGUGACAGUCCCGUAUUCGAAAUCUCAACAGCGGUACUAAGCGUACGAAAAGUAGGGCGGGACACGUGAAAUCCUGUCUGAAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCAUCAUCGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGUGAAACAGAACCUGAAACCUGAUGCAUACAAACAGUGGGAGCGCCCUAGUGGUGUGACUGCGUACCUUUUGUAUAAUGGGUCAACGACUUACAUUCAGUAGCGAGCUUAACCGGAUAGGGGAGGCGUAGGGAAACCGAGUCUUAAUAGGGCGAUGAGUUGCUGGGUGUAGACCCGAAACCGAGUGAUCUAUCCAUGGCCAGGUUGAAGGUGCCGUAACAGGUACUGGAGGACCGAACCCACGCAUGUUGCAAAAUGCGGGGAUGAGCUGUGGGUAGGGGUGAAAGGCUAAACAAACUCGGAGAUAGCUGGUUCUCCCCGAAAACUAUUUAGGUAGUGCCUCGAGCAAGACACUGAUGGGGGUAAAGCACUGUUAUGGCUAGGGGGUUAUUGCAACUUACCAACCCAUGGCAAACUCAGAAUACCAUCAAGUGGUUCCUCGGGAGACAGACAGCGGGUGCUAACGUCCGUUGUCAAGAGGGAAACAACCCAGACCGCCGGCUAAGGUCCCAAAUGAUAGAUUAAGUGGUAAACGAAGUGGGAAGGCACAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGUCCUGCGCGGAAGAUGUAACGGGGCUCAAAUCUAUAACCGAAGCUGCGGAUGCCGGUUUACCGGCAUGGUAGGGGAGCGUUCUGUAGGCUGAUGAAGGUGCAUUGUAAAGUGUGCUGGAGGUAUCAGAAGUGCGAAUGUUGACAUGAGUAGCGAUAAAGCGGGUGAAAAGCCCGCUCGCCGAAAGCCCAAGGUUUCCUACGCAACGUUCAUCGGCGUAGGGUGAGUCGGCCCCUAAGGCGAGGCAGAAAUGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGAUUCAAAUGCGAUGUGGGGACGGAGAAGGUUAGGUUGGCAAGCUGUUGGAAUAGCUUGUUUAAGCCGGUAGGUGGAAGACUUAGGCAAAUCCGGGUUUUCUUAACACCGAGAAGUGAUGACGAGUGUCUACGGACACGAAGCAACCGAUACCACGCUUCCAGGAAAAGCCACUAAGCUUCAGUUUGAAUCGAACCGUACCGCAAACCGACACAGGUGGGCAGGAUGAGAAUUCUAAGGCGCUUGAGAGAACUCGGGAGAAGGAACUCGGCAAAUUGAUACCGUAACUUCGGGAGAAGGUAUGCCCUCUAAGGUUAAGGACUUGCUCCGUAAGCCCCGGAGGGUCGCAGAGAAUAGGUGGCUGCGACUGUUUAUUAAAAACACAGCACUCUGCCAACACGAAAGUGGACGUAUAGGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAAGAUGUGCAAGCAUCGGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAUGGCGUAACGAUGGCCACACUGUCUCCUCCCGAGACUCAGCGAAGUUGAAGUGGUUGUGAAGAUGCAAUCUACCCGCUGCUAGACGGAAAGACCCCGUGAACCUUUACUGUAGCUUUGCAUUGGACUUUGAAGUCACUUGUGUAGGAUAGGUGGGAGGCUUGGAAGCAGAGACGCCAGUCUCUGUGGAGUCGUCCUUGAAAUACCACCCUGGUGUCUUUGAGGUUCUAACCCAGACCCGUCAUCCGGGUCGGGGACCGUGCAUGGUAGGCAGUUUGACUGGGGCGGUCUCCUCCCAAAGCGUAACGGAGGAGUUCGAAGGUUACCUAGGUCCGGUCGGAAAUCGGACUGAUAGUGCAAUGGCAAAAGGUAGCUUAACUGCGAGACCGACAAGUCGGGCAGGUGCGAAAGCAGGACAUAGUGAUCCGGUGGUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUUCCGCCCAAGAGUUCAUAUCGACGGCGGAGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGUAGUCGGUCCCAAGGGUAUGGCUGUUCGCCAUUUAAAGUGGUACGUGAGCUGGGUUUAAAACGUCGUGAGACAGUUUGGUCCCUAUCUGCAGUGGGCGUUGGAAGUUUGACGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGAACCUCUGGUGUACCGGUUGUAACGCCAGUUGCAUAGCCGGGUAGCUAAGUUCGGAAGAGAUAAGCGCUGAAAGCAUCUAAGCGCGAAACUCGCCUGAAGAUGAGACUUCCCUUGCGGUUUAACCGCACUAAAGGGUCGUUCGAGACCAGGACGUUGAUAGGUGGGGUGUGGAAGCGCGGUAACGCGUGAAGCUAACCCAUACUAAUUGCCCGUGAGGCUUGACUCU

> A9Y61_09330: 16S ribosomal RNA (1 of 4 copies)NZ_CP016017.1:1722390-17239411721792 — Is on the negative strand DNA (-strand): SEQ ID NO: 19

TGAACATAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCAGCACAGGGAAGCTTGCTTCTCGGGTGGCGAGTGGCGAACGGGTGAGTAACATATCGGAACGTACCGGGTAGCGGGGGATAACTGATCGAAAGATCAGCTAATACCGCATACGTCTTGAGAGGGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCCGAGCGGCCGATATCTGATTAGCTGGTTGGCGGGGTAAAGGCCCACCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTCTGAAGAAGGCCTTCGGGTTGTAAAGGACTTTTGTCAGGGAAGAAAAGGCCGTTGCCAATATCGGCGGCCGATGACGGTACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTTAAGCAGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCTGAACTGGGTGACTCGAGTGTGTCAGAGGGAGGTGGAATTCCACGTGTAGCAGTGAAATGCGTAGAGATGTGGAGGAATACCGATGGCGAAGGCAGCCTCCTGGGATAACACTGACGTTCATGTCCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAATTAGCTGTTGGGCAACTTGATTGCTTGGTAGCGTAGCTAACGCGTGAAATTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAGAACCTTACCTGGTTTTGACATGTGCGGAATCCTCCGGAGACGGAGGAGTGCCTTCGGGAGCCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCATTCGGTTGGGCACTCTAATGAGACTGCCGGTGACAAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGACCAGGGCTTCACACGTCATACAATGGTCGGTACAGAGGGTAGCCAAGCCGCGAGGCGGAGCCAATCTCACAAAACCGATCGTAGTCCGGATTGCACTCTGCAACTCGAGTGCATGAAGTCGGAATCGCTAGTAATCGCAGGTCAGCATACTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAGTCCGCTTACCACGGTATGCTTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCAC CTCCTTTCTA

cDNA: SEQ ID NO: 20

TAGAAAGGAGGTGATCCAGCCGCAGGTTCCCCTACGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCGGACTCCTTGCGGTTACCCTACCTACTTCTGGTATCCCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCGACTTCATGCACTCGAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTGTGAGATTGGCTCCGCCTCGCGGCTTGGCTACCCTCTGTACCGACCATTGTATGACGTGTGAAGCCCTGGTCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGCTTGTCACCGGCAGTCTCATTAGAGTGCCCAACCGAATGATGGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTACGGCTCCCGAAGGCACTCCTCCGTCTCCGGAGGATTCCGCACATGTCAAAACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCACATCATCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACGCTACCAAGCAATCAAGTTGCCCAACAGCTAATTGACATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGACATGAACGTCAGTGTTATCCCAGGAGGCTGCCTTCGCCATCGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGAATTCCACCTCCCTCTGACACACTCGAGTCACCCAGTTCAGAACGCAGTTCCCGGGTTGAGCCCGGGGATTTCACATCCTGCTTAAGTAACCGTCTGCGCCCGCTTTACGCCCAGTAATTCCGATTAACGCTCGCACCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACCGTCATCGGCCGCCGATATTGGCAACGGCCTTTTCTTCCCTGACAAAAGTCCTTTACAACCCGAAGGCCTTCTTCAGACACGCGGCATGGCTGGATCAGGCTTGCGCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCTACTGATCGTCGCCTTGGTGGGCCTTTACCCCGCCAACCAGCTAATCAGATATCGGCCGCTCGGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCCCTCTCAAGACGTATGCGGTATTAGCTGATCTTTCGATCAGTTATCCCCCGCTACCCGGTACGTTCCGATATGTTACTCACCCGTTCGCCACTCGCCACCCGAGAAGCAAGCTTCCCTGTGCTGCCGTCCGACTTGCATGTGTAAAGCATGCCGCCAGCGTTCAATCTGAGCCAGGATCAAACTCTTATGTTCA

RNA: SEQ ID NO: 21

UGAACAUAAGAGUUUGAUCCUGGCUCAGAUUGAACGCUGGCGGCAUGCUUUACACAUGCAAGUCGGACGGCAGCACAGGGAAGCUUGCUUCUCGGGUGGCGAGUGGCGAACGGGUGAGUAACAUAUCGGAACGUACCGGGUAGCGGGGGAUAACUGAUCGAAAGAUCAGCUAAUACCGCAUACGUCUUGAGAGGGAAAGCAGGGGACCUUCGGGCCUUGCGCUAUCCGAGCGGCCGAUAUCUGAUUAGCUGGUUGGCGGGGUAAAGGCCCACCAAGGCGACGAUCAGUAGCGGGUCUGAGAGGAUGAUCCGCCACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUUUUGGACAAUGGGCGCAAGCCUGAUCCAGCCAUGCCGCGUGUCUGAAGAAGGCCUUCGGGUUGUAAAGGACUUUUGUCAGGGAAGAAAAGGCCGUUGCCAAUAUCGGCGGCCGAUGACGGUACCUGAAGAAUAAGCACCGGCUAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGGUGCGAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGGGCGCAGACGGUUACUUAAGCAGGAUGUGAAAUCCCCGGGCUCAACCCGGGAACUGCGUUCUGAACUGGGUGACUCGAGUGUGUCAGAGGGAGGUGGAAUUCCACGUGUAGCAGUGAAAUGCGUAGAGAUGUGGAGGAAUACCGAUGGCGAAGGCAGCCUCCUGGGAUAACACUGACGUUCAUGUCCGAAAGCGUGGGUAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCCUAAACGAUGUCAAUUAGCUGUUGGGCAACUUGAUUGCUUGGUAGCGUAGCUAACGCGUGAAAUUGACCGCCUGGGGAGUACGGUCGCAAGAUUAAAACUCAAAGGAAUUGACGGGGACCCGCACAAGCGGUGGAUGAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUUUUGACAUGUGCGGAAUCCUCCGGAGACGGAGGAGUGCCUUCGGGAGCCGUAACACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUGUCAUUAGUUGCCAUCAUUCGGUUGGGCACUCUAAUGAGACUGCCGGUGACAAGCCGGAGGAAGGUGGGGAUGACGUCAAGUCCUCAUGGCCCUUAUGACCAGGGCUUCACACGUCAUACAAUGGUCGGUACAGAGGGUAGCCAAGCCGCGAGGCGGAGCCAAUCUCACAAAACCGAUCGUAGUCCGGAUUGCACUCUGCAACUCGAGUGCAUGAAGUCGGAAUCGCUAGUAAUCGCAGGUCAGCAUACUGCGGUGAAUACGUUCCCGGGUCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGGGAUACCAGAAGUAGGUAGGGUAACCGCAAGGAGUCCGCUUACCACGGUAUGCUUCAUGACUGGGGUGAAGUCGUAACAAGGUAGCCGUAGGGGAACCUGCGGCUGGAUCACCUCCUUUCU A

> A9Y61_10490: 23S ribosomal RNA (1 of 4 copies)NZ_CP016017.1:1941315-1944213 - Is on the negative strand DNA (-strand): SEQ ID NO: 22

TGAAATGATAGAGTCAAGTGAATAAGTGCATCAGGCGGATGCCTTGGCGATGATAGGCGACGAAGGACGTGTAAGCCTGCGAAAAGCGCGGGGGAGCTGGCAATAAAGCAATGATCCCGCGGTGTCCGAATGGGGAAACCCACTGCATTCTGTGCAGTATCCTAAGTTGAATACATAGGCTTAGAGAAGCGAACCCGGAGAACTGACCCATCTAAGTACCCGGAGGAAAAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAACGCGGAGGAGCCTGTACGTAATAACTGTCGAGGTAGAAGAACAAGCTGGGAAGCTTGACCATAGCGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCGTACGAAAAGTAGGGCGGGACACGTGAAATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCATCATCGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAACAGAACCTGAAACCTGATGCATACAAACAGTGGGAGCGCCCTAGTGGTGTGACTGCGTACCTTTTGTATAATGGGTCAACGACTTACATTCAGTAGCGAGCTTAACCGGATAGGGGAGGCGTAGGGAAACCGAGTCTTAATAGGGCGATGAGTTGCTGGGTGTAGACCCGAAACCGAGTGATCTATCCATGGCCAGGTTGAAGGTGCCGTAACAGGTACTGGAGGACCGAACCCACGCATGTTGCAAAATGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCTAAACAAACTCGGAGATAGCTGGTTCTCCCCGAAAACTATTTAGGTAGTGCCTCGAGCAAGACACTGATGGGGGTAAAGCACTGTTATGGCTAGGGGGTTATTGCAACTTACCAACCCATGGCAAACTCAGAATACCATCAAGTGGTTCCTCGGGAGACAGACAGCGGGTGCTAACGTCCGTTGTCAAGAGGGAAACAACCCAGACCGCCGGCTAAGGTCCCAAATGATAGATTAAGTGGTAAACGAAGTGGGAAGGCACAGACAGCCAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGTCCTGCGCGGAAGATGTAACGGGGCTCAAATCTATAACCGAAGCTGCGGATGCCGGTTTACCGGCATGGTAGGGGAGCGTTCTGTAGGCTGATGAAGGTGCATTGTAAAGTGTGCTGGAGGTATCAGAAGTGCGAATGTTGACATGAGTAGCGATAAAGCGGGTGAAAAGCCCGCTCGCCGAAAGCCCAAGGTTTCCTACGCAACGTTCATCGGCGTAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAAATGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGATTCAAATGCGATGTGGGGACGGAGAAGGTTAGGTTGGCAAGCTGTTGGAATAGCTTGTTTAAGCCGGTAGGTGGAAGACTTAGGCAAATCCGGGTTTTCTTAACACCGAGAAGTGATGACGAGTGTCTACGGACACGAAGCAACCGATACCACGCTTCCAGGAAAAGCCACTAAGCTTCAGTTTGAATCGAACCGTACCGCAAACCGACACAGGTGGGCAGGATGAGAATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGATACCGTAACTTCGGGAGAAGGTATGCCCTCTAAGGTTAAGGACTTGCTCCGTAAGCCCCGGAGGGTCGCAGAGAATAGGTGGCTGCGACTGTTTATTAAAAACACAGCACTCTGCCAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGAAGATGTGCAAGCATCGGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAATGGCGTAACGATGGCCACACTGTCTCCTCCCGAGACTCAGCGAAGTTGAAGTGGTTGTGAAGATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGTCACTTGTGTAGGATAGGTGGGAGGCTTGGAAGCAGAGACGCCAGTCTCTGTGGAGTCGTCCTTGAAATACCACCCTGGTGTCTTTGAGGTTCTAACCCAGACCCGTCATCCGGGTCGGGGACCGTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGCGTAACGGAGGAGTTCGAAGGTTACCTAGGTCCGGTCGGAAATCGGACTGATAGTGCAATGGCAAAAGGTAGCTTAACTGCGAGACCGACAAGTCGGGCAGGTGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATTCCGCCCAAGAGTTCATATCGACGGCGGAGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGTAGTCGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGTGAGCTGGGTTTAAAACGTCGTGAGACAGTTTGGTCCCTATCTGCAGTGGGCGTTGGAAGTTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGAACCTCTGGTGTACCGGTTGTAACGCCAGTTGCATAGCCGGGTAGCTAAGTTCGGAAGAGATAAGCGCTGAAAGCATCTAAGCGCGAAACTCGCCTGAAGATGAGACTTCCCTTGCGGTTTAACCGCACTAAAGGGTCGTTCGAGACCAGGACGTTGATAGGTGGGGTGTGGAAGCGCGGTAACGCGTGAAGCTAACCCATACTAATTGCCCGTGAGGCTTGACTCT

cDNA: SEQ ID NO: 23

AGAGTCAAGCCTCACGGGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACACCCCACCTATCAACGTCCTGGTCTCGAACGACCCTTTAGTGCGGTTAAACCGCAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCGCTTAGATGCTTTCAGCGCTTATCTCTTCCGAACTTAGCTACCCGGCTATGCAACTGGCGTTACAACCGGTACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCGTCAAACTTCCAACGCCCACTGCAGATAGGGACCAAACTGTCTCACGACGTTTTAAACCCAGCTCACGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTACAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACTCCGCCGTCGATATGAACTCTTGGGCGGAATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTATGTCCTGCTTTCGCACCTGCCCGACTTGTCGGTCTCGCAGTTAAGCTACCTTTTGCCATTGCACTATCAGTCCGATTTCCGACCGGACCTAGGTAACCTTCGAACTCCTCCGTTACGCTTTGGGAGGAGACCGCCCCAGTCAAACTGCCTACCATGCACGGTCCCCGACCCGGATGACGGGTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGACTCCACAGAGACTGGCGTCTCTGCTTCCAAGCCTCCCACCTATCCTACACAAGTGACTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCAGCGGGTAGATTGCATCTTCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCCGATGCTTGCACATCTTCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTGGCAGAGTGCTGTGTTTTTAATAAACAGTCGCAGCCACCTATTCTCTGCGACCCTCCGGGGCTTACGGAGCAAGTCCTTAACCTTAGAGGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAATTCTCATCCTGCCCACCTGTGTCGGTTTGCGGTACGGTTCGATTCAAACTGAAGCTTAGTGGCTTTTCCTGGAAGCGTGGTATCGGTTGCTTCGTGTCCGTAGACACTCGTCATCACTTCTCGGTGTTAAGAAAACCCGGATTTGCCTAAGTCTTCCACCTACCGGCTTAAACAAGCTATTCCAACAGCTTGCCAACCTAACCTTCTCCGTCCCCACATCGCATTTGAATCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCATTTCTGCCTCGCCTTAGGGGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTTCGGCGAGCGGGCTTTTCACCCGCTTTATCGCTACTCATGTCAACATTCGCACTTCTGATACCTCCAGCACACTTTACAATGCACCTTCATCAGCCTACAGAACGCTCCCCTACCATGCCGGTAAACCGGCATCCGCAGCTTCGGTTATAGATTTGAGCCCCGTTACATCTTCCGCGCAGGACGACTCGACCAGTGAGCTATTACGCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGTGCCTTCCCACTTCGTTTACCACTTAATCTATCATTTGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTTGACAACGGACGTTAGCACCCGCTGTCTGTCTCCCGAGGAACCACTTGATGGTATTCTGAGTTTGCCATGGGTTGGTAAGTTGCAATAACCCCCTAGCCATAACAGTGCTTTACCCCCATCAGTGTCTTGCTCGAGGCACTACCTAAATAGTTTTCGGGGAGAACCAGCTATCTCCGAGTTTGTTTAGCCTTTCACCCCTACCCACAGCTCATCCCCGCATTTTGCAACATGCGTGGGTTCGGTCCTCCAGTACCTGTTACGGCACCTTCAACCTGGCCATGGATAGATCACTCGGTTTCGGGTCTACACCCAGCAACTCATCGCCCTATTAAGACTCGGTTTCCCTACGCCTCCCCTATCCGGTTAAGCTCGCTACTGAATGTAAGTCGTTGACCCATTATACAAAAGGTACGCAGTCACACCACTAGGGCGCTCCCACTGTTTGTATGCATCAGGTTTCAGGTTCTGTTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCGATGATGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATTTCACGTGTCCCGCCCTACTTTTCGTACGCTTAGTACCGCTGTTGAGATTTCGAATACGGGACTGTCACCCGCTATGGTCAAGCTTCCCAGCTTGTTCTTCTACCTCGACAGTTATTACGTACAGGCTCCTCCGCGTTCGCTCGCCACTACTTGCGGAATCTCGGTTGATTTCTTTTCCTCCGGGTACTTAGATGGGTCAGTTCTCCGGGTTCGCTTCTCTAAGCCTATGTATTCAACTTAGGATACTGCACAGAATGCAGTGGGTTTCCCCATTCGGACACCGCGGGATCATTGCTTTATTGCCAGCTCCCCCGCGCTTTTCGCAGGCTTACACGTCCTTCGTCGCCTATCATCGCCAAGGCATCCGCCTGATGCACTTATTCACTTGACTCTATCATTTCA

RNA: SEQ ID NO: 24

UGAAAUGAUAGAGUCAAGUGAAUAAGUGCAUCAGGCGGAUGCCUUGGCGAUGAUAGGCGACGAAGGACGUGUAAGCCUGCGAAAAGCGCGGGGGAGCUGGCAAUAAAGCAAUGAUCCCGCGGUGUCCGAAUGGGGAAACCCACUGCAUUCUGUGCAGUAUCCUAAGUUGAAUACAUAGGCUUAGAGAAGCGAACCCGGAGAACUGACCCAUCUAAGUACCCGGAGGAAAAGAAAUCAACCGAGAUUCCGCAAGUAGUGGCGAGCGAACGCGGAGGAGCCUGUACGUAAUAACUGUCGAGGUAGAAGAACAAGCUGGGAAGCUUGACCAUAGCGGGUGACAGUCCCGUAUUCGAAAUCUCAACAGCGGUACUAAGCGUACGAAAAGUAGGGCGGGACACGUGAAAUCCUGUCUGAAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCAUCAUCGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGUGAAACAGAACCUGAAACCUGAUGCAUACAAACAGUGGGAGCGCCCUAGUGGUGUGACUGCGUACCUUUUGUAUAAUGGGUCAACGACUUACAUUCAGUAGCGAGCUUAACCGGAUAGGGGAGGCGUAGGGAAACCGAGUCUUAAUAGGGCGAUGAGUUGCUGGGUGUAGACCCGAAACCGAGUGAUCUAUCCAUGGCCAGGUUGAAGGUGCCGUAACAGGUACUGGAGGACCGAACCCACGCAUGUUGCAAAAUGCGGGGAUGAGCUGUGGGUAGGGGUGAAAGGCUAAACAAACUCGGAGAUAGCUGGUUCUCCCCGAAAACUAUUUAGGUAGUGCCUCGAGCAAGACACUGAUGGGGGUAAAGCACUGUUAUGGCUAGGGGGUUAUUGCAACUUACCAACCCAUGGCAAACUCAGAAUACCAUCAAGUGGUUCCUCGGGAGACAGACAGCGGGUGCUAACGUCCGUUGUCAAGAGGGAAACAACCCAGACCGCCGGCUAAGGUCCCAAAUGAUAGAUUAAGUGGUAAACGAAGUGGGAAGGCACAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGUCCUGCGCGGAAGAUGUAACGGGGCUCAAAUCUAUAACCGAAGCUGCGGAUGCCGGUUUACCGGCAUGGUAGGGGAGCGUUCUGUAGGCUGAUGAAGGUGCAUUGUAAAGUGUGCUGGAGGUAUCAGAAGUGCGAAUGUUGACAUGAGUAGCGAUAAAGCGGGUGAAAAGCCCGCUCGCCGAAAGCCCAAGGUUUCCUACGCAACGUUCAUCGGCGUAGGGUGAGUCGGCCCCUAAGGCGAGGCAGAAAUGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGAUUCAAAUGCGAUGUGGGGACGGAGAAGGUUAGGUUGGCAAGCUGUUGGAAUAGCUUGUUUAAGCCGGUAGGUGGAAGACUUAGGCAAAUCCGGGUUUUCUUAACACCGAGAAGUGAUGACGAGUGUCUACGGACACGAAGCAACCGAUACCACGCUUCCAGGAAAAGCCACUAAGCUUCAGUUUGAAUCGAACCGUACCGCAAACCGACACAGGUGGGCAGGAUGAGAAUUCUAAGGCGCUUGAGAGAACUCGGGAGAAGGAACUCGGCAAAUUGAUACCGUAACUUCGGGAGAAGGUAUGCCCUCUAAGGUUAAGGACUUGCUCCGUAAGCCCCGGAGGGUCGCAGAGAAUAGGUGGCUGCGACUGUUUAUUAAAAACACAGCACUCUGCCAACACGAAAGUGGACGUAUAGGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAAGAUGUGCAAGCAUCGGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAUGGCGUAACGAUGGCCACACUGUCUCCUCCCGAGACUCAGCGAAGUUGAAGUGGUUGUGAAGAUGCAAUCUACCCGCUGCUAGACGGAAAGACCCCGUGAACCUUUACUGUAGCUUUGCAUUGGACUUUGAAGUCACUUGUGUAGGAUAGGUGGGAGGCUUGGAAGCAGAGACGCCAGUCUCUGUGGAGUCGUCCUUGAAAUACCACCCUGGUGUCUUUGAGGUUCUAACCCAGACCCGUCAUCCGGGUCGGGGACCGUGCAUGGUAGGCAGUUUGACUGGGGCGGUCUCCUCCCAAAGCGUAACGGAGGAGUUCGAAGGUUACCUAGGUCCGGUCGGAAAUCGGACUGAUAGUGCAAUGGCAAAAGGUAGCUUAACUGCGAGACCGACAAGUCGGGCAGGUGCGAAAGCAGGACAUAGUGAUCCGGUGGUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUUCCGCCCAAGAGUUCAUAUCGACGGCGGAGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGUAGUCGGUCCCAAGGGUAUGGCUGUUCGCCAUUUAAAGUGGUACGUGAGCUGGGUUUAAAACGUCGUGAGACAGUUUGGUCCCUAUCUGCAGUGGGCGUUGGAAGUUUGACGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGAACCUCUGGUGUACCGGUUGUAACGCCAGUUGCAUAGCCGGGUAGCUAAGUUCGGAAGAGAUAAGCGCUGAAAGCAUCUAAGCGCGAAACUCGCCUGAAGAUGAGACUUCCCUUGCGGUUUAACCGCACUAAAGGGUCGUUCGAGACCAGGACGUUGAUAGGUGGGGUGUGGAAGCGCGGUAACGCGUGAAGCUAACCCAUACUAAUUGCCCGUGAGGCUUGACUCU

> A9Y61_10505: 16S ribosomal RNA (1 of 4 copies)NZ_CP016017.1:1944811-1946362- Is on the negative strand DNA (- strand):SEQ ID NO: 25

TGAACATAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCAGCACAGGGAAGCTTGCTTCTCGGGTGGCGAGTGGCGAACGGGTGAGTAACATATCGGAACGTACCGGGTAGCGGGGGATAACTGATCGAAAGATCAGCTAATACCGCATACGTCTTGAGAGGGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCCGAGCGGCCGATATCTGATTAGCTGGTTGGCGGGGTAAAGGCCCACCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTCTGAAGAAGGCCTTCGGGTTGTAAAGGACTTTTGTCAGGGAAGAAAAGGCCGTTGCCAATATCGGCGGCCGATGACGGTACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTTAAGCAGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCTGAACTGGGTGACTCGAGTGTGTCAGAGGGAGGTGGAATTCCACGTGTAGCAGTGAAATGCGTAGAGATGTGGAGGAATACCGATGGCGAAGGCAGCCTCCTGGGATAACACTGACGTTCATGTCCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAATTAGCTGTTGGGCAACTTGATTGCTTGGTAGCGTAGCTAACGCGTGAAATTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAGAACCTTACCTGGTTTTGACATGTGCGGAATCCTCCGGAGACGGAGGAGTGCCTTCGGGAGCCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCATTCGGTTGGGCACTCTAATGAGACTGCCGGTGACAAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGACCAGGGCTTCACACGTCATACAATGGTCGGTACAGAGGGTAGCCAAGCCGCGAGGCGGAGCCAATCTCACAAAACCGATCGTAGTCCGGATTGCACTCTGCAACTCGAGTGCATGAAGTCGGAATCGCTAGTAATCGCAGGTCAGCATACTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAGTCCGCTTACCACGGTATGCTTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCAC CTCCTTTCTA

cDNA: SEQ ID NO: 26

TAGAAAGGAGGTGATCCAGCCGCAGGTTCCCCTACGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCGGACTCCTTGCGGTTACCCTACCTACTTCTGGTATCCCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCGACTTCATGCACTCGAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTGTGAGATTGGCTCCGCCTCGCGGCTTGGCTACCCTCTGTACCGACCATTGTATGACGTGTGAAGCCCTGGTCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGCTTGTCACCGGCAGTCTCATTAGAGTGCCCAACCGAATGATGGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTACGGCTCCCGAAGGCACTCCTCCGTCTCCGGAGGATTCCGCACATGTCAAAACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCACATCATCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACGCTACCAAGCAATCAAGTTGCCCAACAGCTAATTGACATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGACATGAACGTCAGTGTTATCCCAGGAGGCTGCCTTCGCCATCGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGAATTCCACCTCCCTCTGACACACTCGAGTCACCCAGTTCAGAACGCAGTTCCCGGGTTGAGCCCGGGGATTTCACATCCTGCTTAAGTAACCGTCTGCGCCCGCTTTACGCCCAGTAATTCCGATTAACGCTCGCACCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACCGTCATCGGCCGCCGATATTGGCAACGGCCTTTTCTTCCCTGACAAAAGTCCTTTACAACCCGAAGGCCTTCTTCAGACACGCGGCATGGCTGGATCAGGCTTGCGCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCTACTGATCGTCGCCTTGGTGGGCCTTTACCCCGCCAACCAGCTAATCAGATATCGGCCGCTCGGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCCCTCTCAAGACGTATGCGGTATTAGCTGATCTTTCGATCAGTTATCCCCCGCTACCCGGTACGTTCCGATATGTTACTCACCCGTTCGCCACTCGCCACCCGAGAAGCAAGCTTCCCTGTGCTGCCGTCCGACTTGCATGTGTAAAGCATGCCGCCAGCGTTCAATCTGAGCCAGGATCAAACTCTTATGTTCA

RNA: SEQ ID NO: 27

UGAACAUAAGAGUUUGAUCCUGGCUCAGAUUGAACGCUGGCGGCAUGCUUUACACAUGCAAGUCGGACGGCAGCACAGGGAAGCUUGCUUCUCGGGUGGCGAGUGGCGAACGGGUGAGUAACAUAUCGGAACGUACCGGGUAGCGGGGGAUAACUGAUCGAAAGAUCAGCUAAUACCGCAUACGUCUUGAGAGGGAAAGCAGGGGACCUUCGGGCCUUGCGCUAUCCGAGCGGCCGAUAUCUGAUUAGCUGGUUGGCGGGGUAAAGGCCCACCAAGGCGACGAUCAGUAGCGGGUCUGAGAGGAUGAUCCGCCACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUUUUGGACAAUGGGCGCAAGCCUGAUCCAGCCAUGCCGCGUGUCUGAAGAAGGCCUUCGGGUUGUAAAGGACUUUUGUCAGGGAAGAAAAGGCCGUUGCCAAUAUCGGCGGCCGAUGACGGUACCUGAAGAAUAAGCACCGGCUAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGGUGCGAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGGGCGCAGACGGUUACUUAAGCAGGAUGUGAAAUCCCCGGGCUCAACCCGGGAACUGCGUUCUGAACUGGGUGACUCGAGUGUGUCAGAGGGAGGUGGAAUUCCACGUGUAGCAGUGAAAUGCGUAGAGAUGUGGAGGAAUACCGAUGGCGAAGGCAGCCUCCUGGGAUAACACUGACGUUCAUGUCCGAAAGCGUGGGUAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCCUAAACGAUGUCAAUUAGCUGUUGGGCAACUUGAUUGCUUGGUAGCGUAGCUAACGCGUGAAAUUGACCGCCUGGGGAGUACGGUCGCAAGAUUAAAACUCAAAGGAAUUGACGGGGACCCGCACAAGCGGUGGAUGAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUUUUGACAUGUGCGGAAUCCUCCGGAGACGGAGGAGUGCCUUCGGGAGCCGUAACACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUGUCAUUAGUUGCCAUCAUUCGGUUGGGCACUCUAAUGAGACUGCCGGUGACAAGCCGGAGGAAGGUGGGGAUGACGUCAAGUCCUCAUGGCCCUUAUGACCAGGGCUUCACACGUCAUACAAUGGUCGGUACAGAGGGUAGCCAAGCCGCGAGGCGGAGCCAAUCUCACAAAACCGAUCGUAGUCCGGAUUGCACUCUGCAACUCGAGUGCAUGAAGUCGGAAUCGCUAGUAAUCGCAGGUCAGCAUACUGCGGUGAAUACGUUCCCGGGUCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGGGAUACCAGAAGUAGGUAGGGUAACCGCAAGGAGUCCGCUUACCACGGUAUGCUUCAUGACUGGGGUGAAGUCGUAACAAGGUAGCCGUAGGGGAACCUGCGGCUGGAUCACCUCCUUUCU A

ANNEX B

Sequences for the exemplary marker genes differentially expressedbetween an untreated sample and a sample treated with antibiotics

1. porB (Locus Tag: NGO1812) Ngo1812: Nc_002946.2:1788697-1789744

DNA (+ strand): SEQ ID NO: 28

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAATGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATACAGACGGCAAGGTAAGTAAAGTGGAAACCGGCAGCGAAATCGCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGTGCCTCCGTCGCCGGCACTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAGGGCGGCTTCGGTACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCGGCGCCAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCAAAACAGCGGCTTCTTCGCGCAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACGATGGTCAAACTTATAGTATCCCCAGTCTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTAGGCGGTTACGACAATAATGCCCTGTACGTTTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGCGGCATACCGTTTCGGCAATGTAACGCCCCGCGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATAGTGCAAACCACGACAATACTTATGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

cDNA: SEQ ID NO: 29

TTAGAATTTGTGGCGCAGAACGACGGCGCTGGCAGTCGATACGATTTTGTCTGCGCCTTTGCCTTCTTGCAACCAGCCGGCAGAAACCAAGGCAGAAGTGCGTTTGGAGAAGTCGTATTCCGCACCGACAACCACTTGGTCATAAGTATTGTCGTGGTTTGCACTATCAACAGTGCCTTTGAAGCCGTGGGCGTAAGAAACGCGGGGCGTTACATTGCCGAAACGGTATGCCGCGGTAGCGGCAACTTCGGTTTGAGAGTTGTGCGAATTACCGCTCATTGCTCCATACAATTTGGCATCTTGTTGTTGTGCGGCTACGGAAACGTACAGGGCATTATTGTCGTAACCGCCTACCAAACGGTGAACTTGCAGTTTTTCAACAAACAGACTGGGGATACTATAAGTTTGACCATCGTATTCGATTTTTTTAGTGCCTTCGCCGTATCTTTGGAACAAGCCGGCGTATTGCGCGAAGAAGCCGCTGTTTTGGTAGTTCAAGCCAACGTGGTAAGATTCGCCGTTTGAGCCTGAATTGTCTTTAGGTGCGTATTGTACGCTGCCGCTGAAGCCGGCAAATTCGGGAGAATCGTAGCGTACGGACAGGTAGCGGTGTTCCCGTTGGGCCATTCCGCTGATTTCCAGCACATTGCCGGTAAATTTGCCGGATTCCCAAGCATTGACGTTGGCGCCGGTGTTTTTCAGGGGGCTGTTCAGGCTACCGGCGCGGATGGTACCGAAGCCGCCCTTCAAGCCGACGAAGGATTGTTTGTTGCCCCAGCCGGTGTTAGTGCCGGCGACGGAGGCACCTTGTTCCAACTGCCAAACGGCCTTCAGGCCGTTGCCGAGGTCTTCTTGGCCTTTGAAGCCGATTTTTGAACCGAAGTCGGCGATTTCGCTGCCGGTTTCCACTTTACTTACCTTGCCGTCTGTATGTTCTACAGAACGGTAAGTTTGTACGCCGGCTTTGATGGCGCCGTACAGGGTGACATCGGCCATTGCCGCAACAGGAAGGGCTGCCAAAGTCAGGGCAATCAGGGATTTTTTCAT

RNA: SEQ ID NO: 30

AUGAAAAAAUCCCUGAUUGCCCUGACUUUGGCAGCCCUUCCUGUUGCGGCAAUGGCCGAUGUCACCCUGUACGGCGCCAUCAAAGCCGGCGUACAAACUUACCGUUCUGUAGAACAUACAGACGGCAAGGUAAGUAAAGUGGAAACCGGCAGCGAAAUCGCCGACUUCGGUUCAAAAAUCGGCUUCAAAGGCCAAGAAGACCUCGGCAACGGCCUGAAGGCCGUUUGGCAGUUGGAACAAGGUGCCUCCGUCGCCGGCACUAACACCGGCUGGGGCAACAAACAAUCCUUCGUCGGCUUGAAGGGCGGCUUCGGUACCAUCCGCGCCGGUAGCCUGAACAGCCCCCUGAAAAACACCGGCGCCAACGUCAAUGCUUGGGAAUCCGGCAAAUUUACCGGCAAUGUGCUGGAAAUCAGCGGAAUGGCCCAACGGGAACACCGCUACCUGUCCGUACGCUACGAUUCUCCCGAAUUUGCCGGCUUCAGCGGCAGCGUACAAUACGCACCUAAAGACAAUUCAGGCUCAAACGGCGAAUCUUACCACGUUGGCUUGAACUACCAAAACAGCGGCUUCUUCGCGCAAUACGCCGGCUUGUUCCAAAGAUACGGCGAAGGCACUAAAAAAAUCGAAUACGAUGGUCAAACUUAUAGUAUCCCCAGUCUGUUUGUUGAAAAACUGCAAGUUCACCGUUUGGUAGGCGGUUACGACAAUAAUGCCCUGUACGUUUCCGUAGCCGCACAACAACAAGAUGCCAAAUUGUAUGGAGCAAUGAGCGGUAAUUCGCACAACUCUCAAACCGAAGUUGCCGCUACCGCGGCAUACCGUUUCGGCAAUGUAACGCCCCGCGUUUCUUACGCCCACGGCUUCAAAGGCACUGUUGAUAGUGCAAACCACGACAAUACUUAUGACCAAGUGGUUGUCGGUGCGGAAUACGACUUCUCCAAACGCACUUCUGCCUUGGUUUCUGCCGGCUGGUUGCAAGAAGGCAAAGGCGCAGACAAAAUCGUAUCGACUGCCAGCGCCGUCGUUCUGCGCCACAAA UUCUAA

2. rpmB (Locus Tag: NGO1680 NGO1680: NC_002946.2: C1633854-1633621

DNA (- strand): SEQ ID NO: 31

ATGGCACGAGTTTGCAAAGTGACCGGTAAACGCCCGATGTCCGGCAACAACGTATCGCACGCCAACAACAAAACCAAACGCCGTTTTTTGCCCAACTTGCAATCACGTCGTTTTTGGGTAGAAAGTGAAAACCGCTGGGTTCGCCTGCGCGTTTCCAACGCTGCATTGCGTACCATCGACAAAGTAGGCATTGATGTCGTATTGGCTGATTTGCGTGCTCGCGGCGA AGCTTAA

cDNA: SEQ ID NO: 32

TTAAGCTTCGCCGCGAGCACGCAAATCAGCCAATACGACATCAATGCCTACTTTGTCGATGGTACGCAATGCAGCGTTGGAAACGCGCAGGCGAACCCAGCGGTTTTCACTTTCTACCCAAAAACGACGTGATTGCAAGTTGGGCAAAAAACGGCGTTTGGTTTTGTTGTTGGCGTGCGATACGTTGTTGCCGGACATCGGGCGTTTACCGGTCACTTTGCAAACTCG TGCCAT

RNA: SEQ ID NO: 33

AUGGCACGAGUUUGCAAAGUGACCGGUAAACGCCCGAUGUCCGGCAACAACGUAUCGCACGCCAACAACAAAACCAAACGCCGUUUUUUGCCCAACUUGCAAUCACGUCGUUUUUGGGUAGAAAGUGAAAACCGCUGGGUUCGCCUGCGCGUUUCCAACGCUGCAUUGCGUACCAUCGACAAAGUAGGCAUUGAUGUCGUAUUGGCUGAUUUGCGUGC UCGCGGCGAAGCUUAA

3. NGO0016: NC_002946.2:c14431-14081

DNA (- strand): SEQ ID NO: 34

ATGGAAGCCTTCAAAACCCTAATTTGGATTATTAATATTATTTCCGCTTTGGCCGTCATCGTGTTAGTATTGCTCCAACACGGCAAAGGCGCGGATGCCGGCGCGACCTTCGGATCGGGAAGCGGCAGCGCGCAAGGCGTATTCGGCTCTGCCGGCAACGCCAACTTCCTCAGCCGCTCGACCGCCGTTGCAGCAACATTTTTCTTTGCAACCTGCATGGCTATGGTGTATATTCACACCCACACGACAAAACACGGTTTGGACTTCAGCAACATACGACAGACTCAGCAAGCACCCAAACCCGTAAGCAATACCGAACCTTCTGCCCCTGTTCCTCAGCA GCAGAAATAA

cDNA: SEQ ID NO: 35

TTATTTCTGCTGCTGAGGAACAGGGGCAGAAGGTTCGGTATTGCTTACGGGTTTGGGTGCTTGCTGAGTCTGTCGTATGTTGCTGAAGTCCAAACCGTGTTTTGTCGTGTGGGTGTGAATATACACCATAGCCATGCAGGTTGCAAAGAAAAATGTTGCTGCAACGGCGGTCGAGCGGCTGAGGAAGTTGGCGTTGCCGGCAGAGCCGAATACGCCTTGCGCGCTGCCGCTTCCCGATCCGAAGGTCGCGCCGGCATCCGCGCCTTTGCCGTGTTGGAGCAATACTAACACGATGACGGCCAAAGCGGAAATAATATTAATAATCCAAATTAGGGTTTTG AAGGCTTCCAT

RNA: SEQ ID NO: 36

AUGGAAGCCUUCAAAACCCUAAUUUGGAUUAUUAAUAUUAUUUCCGCUUUGGCCGUCAUCGUGUUAGUAUUGCUCCAACACGGCAAAGGCGCGGAUGCCGGCGCGACCUUCGGAUCGGGAAGCGGCAGCGCGCAAGGCGUAUUCGGCUCUGCCGGCAACGCCAACUUCCUCAGCCGCUCGACCGCCGUUGCAGCAACAUUUUUCUUUGCAACCUGCAUGGCUAUGGUGUAUAUUCACACCCACACGACAAAACACGGUUUGGACUUCAGCAACAUACGACAGACUCAGCAAGCACCCAAACCCGUAAGCAAUACCGAACCUUCUGCCCCUGUUCCUCAGCAGCAGAAAUAA

4. NGO0171: NC_002946.2:c174519-174154

DNA (- strand): SEQ ID NO: 37

ATGAACCTGATTCAACAGCTCGAGCAAGAAGAAATTGCCCGCCTGAACAAAGAAATCCCCGAATTCGCACCGGGCGACACCGTAGTCGTATCCGTACGCGTCGTGGAAGGTACCCGCAGCCGTCTGCAAGCCTACGAAGGCGTGGTTATCGCCCGTCGCAACCGTGGTTTGAACAGCAACTTCATCGTCCGCAAAATCTCCAGCGGCGAAGGTGTTGAACGTACTTTCCAACTGTATTCCCCTACTGTTGAGAAAATCGAAGTCAAACGCCGTGGCGACGTACGCCGTGCCAAACTGTACTACCTGCGCGGTCTGACCGGCAAAGCTGCACGCATCAAAGAAAAACTGCCTGCACGCAAAGGTTGA

cDNA: SEQ ID NO: 38

TCAACCTTTGCGTGCAGGCAGTTTTTCTTTGATGCGTGCAGCTTTGCCGGTCAGACCGCGCAGGTAGTACAGTTTGGCACGGCGTACGTCGCCACGGCGTTTGACTTCGATTTTCTCAACAGTAGGGGAATACAGTTGGAAAGTACGTTCAACACCTTCGCCGCTGGAGATTTTGCGGACGATGAAGTTGCTGTTCAAACCACGGTTGCGACGGGCGATAACCACGCCTTCGTAGGCTTGCAGACGGCTGCGGGTACCTTCCACGACGCGTACGGATACGACTACGGTGTCGCCCGGTGCGAATTCGGGGATTTCTTTGTTCAGGCGGGCAATTTCTTCTTGCTCGAGCTGTTGAATCAGGTTCAT

RNA: SEQ ID NO: 39

AUGAACCUGAUUCAACAGCUCGAGCAAGAAGAAAUUGCCCGCCUGAACAAAGAAAUCCCCGAAUUCGCACCGGGCGACACCGUAGUCGUAUCCGUACGCGUCGUGGAAGGUACCCGCAGCCGUCUGCAAGCCUACGAAGGCGUGGUUAUCGCCCGUCGCAACCGUGGUUUGAACAGCAACUUCAUCGUCCGCAAAAUCUCCAGCGGCGAAGGUGUUGAACGUACUUUCCAACUGUAUUCCCCUACUGUUGAGAAAAUCGAAGUCAAACGCCGUGGCGACGUACGCCGUGCCAAACUGUACUACCUGCGCGGUCUGACCGGCAAAGCUGCACGCAUCAAAGAAAAACUGCCUGCACGCAAAGGUUGA

5. NGO0172: NC_002946.2:c175283-174534

DNA (- strand): SEQ ID NO: 40

ATGCTTATCCAGGCAGTTACCATTTTCCCCGAAATGTTCGACAGCATTACCCGCTACGGCGTAACGGGACGCGCGAACAGACAGGGAATCTGGCAGTTTGAAGCAGTCAATCCCCGAAAGTTTGCCGACAACAGATTGGGCTATATCGACGACCGCCCGTTCGGCGGCGGTCCGGGAATGATTATGATGGCTCCGCCGCTTCATGCGGCGATAGAACACGCCAAAGCACAATCTTCCCAAACCGCAAAAGTCATCTACCTCAGCCCCCAAGGAAAACCGCTGACACACCAAAAAGCGGCAGAACTGGCAGAACTTACGCATCTGATTCTGCTGTGCGGACGCTATGAGGGAATAGACGAAAGACTGCTGCAAAGCAGCGTCGATGAAGAAATCAGCATCGGAGACTTCGTCGTTTCCGGCGGAGAGCTTCCCGCCATGATGCTGATGGATGCGGTATTGAGGCTCGTACCCGGCATATTGGGCGACATTCAGTCTGCCGAACAGGATTCGTTCTCAAGCGGTATTTTGGACTGCCCCCACTACACCAAACCCTTAGAATTTCAAGGCATGGCTGTTCCGGAAGTATTGCGCTCCGGAAATCATGGCTTGATAGCGGAATGGCGGTTGGAACAATCGCTGCGCCGCACCTTGGAGCGCAGACCCGATCTTTTGGAAAAGCGCGTTTTAATCCCAAAGGAATCCCGCCTCTTGAATAAAATCCTACAAGAGCAACGGGAAATCCAATCATAA

cDNA: SEQ ID NO: 41

TTATGATTGGATTTCCCGTTGCTCTTGTAGGATTTTATTCAAGAGGCGGGATTCCTTTGGGATTAAAACGCGCTTTTCCAAAAGATCGGGTCTGCGCTCCAAGGTGCGGCGCAGCGATTGTTCCAACCGCCATTCCGCTATCAAGCCATGATTTCCGGAGCGCAATACTTCCGGAACAGCCATGCCTTGAAATTCTAAGGGTTTGGTGTAGTGGGGGCAGTCCAAAATACCGCTTGAGAACGAATCCTGTTCGGCAGACTGAATGTCGCCCAATATGCCGGGTACGAGCCTCAATACCGCATCCATCAGCATCATGGCGGGAAGCTCTCCGCCGGAAACGACGAAGTCTCCGATGCTGATTTCTTCATCGACGCTGCTTTGCAGCAGTCTTTCGTCTATTCCCTCATAGCGTCCGCACAGCAGAATCAGATGCGTAAGTTCTGCCAGTTCTGCCGCTTTTTGGTGTGTCAGCGGTTTTCCTTGGGGGCTGAGGTAGATGACTTTTGCGGTTTGGGAAGATTGTGCTTTGGCGTGTTCTATCGCCGCATGAAGCGGCGGAGCCATCATAATCATTCCCGGACCGCCGCCGAACGGGCGGTCGTCGATATAGCCCAATCTGTTGTCGGCAAACTTTCGGGGATTGACTGCTTCAAACTGCCAGATTCCCTGTCTGTTCGCGCGTCCCGTTACGCCGTAGCGGGTAATGCTGTCGAACATTTCGGGGAAAATGGTAACTGCCTGG ATAAGCAT

RNA: SEQ ID NO: 42

AUGCUUAUCCAGGCAGUUACCAUUUUCCCCGAAAUGUUCGACAGCAUUACCCGCUACGGCGUAACGGGACGCGCGAACAGACAGGGAAUCUGGCAGUUUGAAGCAGUCAAUCCCCGAAAGUUUGCCGACAACAGAUUGGGCUAUAUCGACGACCGCCCGUUCGGCGGCGGUCCGGGAAUGAUUAUGAUGGCUCCGCCGCUUCAUGCGGCGAUAGAACACGCCAAAGCACAAUCUUCCCAAACCGCAAAAGUCAUCUACCUCAGCCCCCAAGGAAAACCGCUGACACACCAAAAAGCGGCAGAACUGGCAGAACUUACGCAUCUGAUUCUGCUGUGCGGACGCUAUGAGGGAAUAGACGAAAGACUGCUGCAAAGCAGCGUCGAUGAAGAAAUCAGCAUCGGAGACUUCGUCGUUUCCGGCGGAGAGCUUCCCGCCAUGAUGCUGAUGGAUGCGGUAUUGAGGCUCGUACCCGGCAUAUUGGGCGACAUUCAGUCUGCCGAACAGGAUUCGUUCUCAAGCGGUAUUUUGGACUGCCCCCACUACACCAAACCCUUAGAAUUUCAAGGCAUGGCUGUUCCGGAAGUAUUGCGCUCCGGAAAUCAUGGCUUGAUAGCGGAAUGGCGGUUGGAACAAUCGCUGCGCCGCACCUUGGAGCGCAGACCCGAUCUUUUGGAAAAGCGCGUUUUAAUCCCAAAGGAAUCCCGCCUCUUGAAUAAAAUCCUACAAGAGCAACGGGAAAUCCAAUCAUAA

6. NGO0173: NC_002946.2:c175792-175283

DNA (- strand): SEQ ID NO: 43

ATGACAGACACTCAAAACCGGGTAGCCATGGGCTACATCAAAGGCGTATTCGGCATAAAAGGCTGGCTGAAAATTGCCGCCAACACCGAATATTCCGACAGCCTTTTGGACTACCCCGAGTGGCATTTGGCCAAGGACGGCAAAACCGTCAGCGTTACCCTTGAAGCCGGAAAAGTCGTCAACGGCGAACTCCAAGTCAAATTCGAAGGCATAGACGACCGCGATTCAGCATTCTCATTGCGCGGTTACACCATCGAAATACCCCGTGAAGCATTCGCCCCGACAGAAGAAGACGAATACTACTGGGCAGACTTGGTCGGCATGACCGTCGTCAACAAAGACGATACCGTTTTAGGCAAGGTAAGCAACCTGATGGAAACCGGCGCAAACGACGTATTGATGATTGACGGAGAACACGGGCAGATTCTGATTCCGTTCGTTTCCCAATATATCGAAACCGTCGATACCGGCAGCAAGACCATTACTGCCGACTGGGGTTTGGACTACT GA

cDNA: SEQ ID NO: 44

TCAGTAGTCCAAACCCCAGTCGGCAGTAATGGTCTTGCTGCCGGTATCGACGGTTTCGATATATTGGGAAACGAACGGAATCAGAATCTGCCCGTGTTCTCCGTCAATCATCAATACGTCGTTTGCGCCGGTTTCCATCAGGTTGCTTACCTTGCCTAAAACGGTATCGTCTTTGTTGACGACGGTCATGCCGACCAAGTCTGCCCAGTAGTATTCGTCTTCTTCTGTCGGGGCGAATGCTTCACGGGGTATTTCGATGGTGTAACCGCGCAATGAGAATGCTGAATCGCGGTCGTCTATGCCTTCGAATTTGACTTGGAGTTCGCCGTTGACGACTTTTCCGGCTTCAAGGGTAACGCTGACGGTTTTGCCGTCCTTGGCCAAATGCCACTCGGGGTAGTCCAAAAGGCTGTCGGAATATTCGGTGTTGGCGGCAATTTTCAGCCAGCCTTTTATGCCGAATACGCCTTTGATGTAGCCCATGGCTACCCGGTTTTGAGTGTCTGTCAT

RNA: SEQ ID NO: 45

AUGACAGACACUCAAAACCGGGUAGCCAUGGGCUACAUCAAAGGCGUAUUCGGCAUAAAAGGCUGGCUGAAAAUUGCCGCCAACACCGAAUAUUCCGACAGCCUUUUGGACUACCCCGAGUGGCAUUUGGCCAAGGACGGCAAAACCGUCAGCGUUACCCUUGAAGCCGGAAAAGUCGUCAACGGCGAACUCCAAGUCAAAUUCGAAGGCAUAGACGACCGCGAUUCAGCAUUCUCAUUGCGCGGUUACACCAUCGAAAUACCCCGUGAAGCAUUCGCCCCGACAGAAGAAGACGAAUACUACUGGGCAGACUUGGUCGGCAUGACCGUCGUCAACAAAGACGAUACCGUUUUAGGCAAGGUAAGCAACCUGAUGGAAACCGGCGCAAACGACGUAUUGAUGAUUGACGGAGAACACGGGCAGAUUCUGAUUCCGUUCGUUUCCCAAUAUAUCGAAACCGUCGAUACCGGCAGCAAGACCAUUACUGCCGACUGGGGUUUGGACUACUGA

7. NGO0174: NC_002946.2:c176053-175808

DNA (- strand): SEQ ID NO: 46

ATGGTAGTTATCCGTTTGGCACGCGGCGGCTCGAAACACCGCCCCTTCTACAACGTCATCGTTACTGACTCACGCAGCCGCCGCGACGGCCGCTTCATCGAACGCGTAGGCTTCTACAACCCCGTAGCCAACGAAAAACAAGAGCGCGTCCGCCTCAATGCAGACCGCCTGAACCACTGGATTGCACAAGGCGCGCAAGTCAGCGACTCCGTTGCAAAACTGATTAAAGAACAAAAAGCCGTCTAA

cDNA: SEQ ID NO: 47

TTAGACGGCTTTTTGTTCTTTAATCAGTTTTGCAACGGAGTCGCTGACTTGCGCGCCTTGTGCAATCCAGTGGTTCAGGCGGTCTGCATTGAGGCGGACGCGCTCTTGTTTTTCGTTGGCTACGGGGTTGTAGAAGCCTACGCGTTCGATGAAGCGGCCGTCGCGGCGGCTGCGTGAGTCAGTAACGATGACGTTGTAGAAGGGGCGGTGTTTCGAGCCGCCGCGTGCCAAACGGATAACTACCAT

RNA: SEQ ID NO: 48

AUGGUAGUUAUCCGUUUGGCACGCGGCGGCUCGAAACACCGCCCCUUCUACAACGUCAUCGUUACUGACUCACGCAGCCGCCGCGACGGCCGCUUCAUCGAACGCGUAGGCUUCUACAACCCCGUAGCCAACGAAAAACAAGAGCGCGUCCGCCUCAAUGCAGACCGCCUGAACCACUGGAUUGCACAAGGCGCGCAAGUCAGCGACUCCGUUGCAAAACUGAUUAAAGAACAAAAAGCCGUCUAA

8. Ngo0340: Nc_002946.2:334760-335692

DNA (+ strand): SEQ ID NO: 49

ATGAAAATTGCAAACAGCATCACCGAATTGATCGGCAACACGCCTTTGGTCAAACTGAACCGTTTGACCAAAGGTTTGAAGGCAGAGGTTGCCGTGAAACTGGAATTTTTTAATCCGGGCAGCAGCGTCAAAGACCGCATTGCCGAAGCAATGATCGAGGCCGCCGAAAAAGCGGGAAAAATCAACAAAAACACCGTCATTGTCGAAGCAACCAGCGGCAATACGGGTATCGGTTTGGCAATGGTATGTGCCGCACGCGGCTACAAACTGGCGATTACCATGCCGGAAAGCATGAGCAAAGAGCGCAAAATGCTGTTGCGCACGTTTGGCGCGGAACTGATTCTAACCCCCGCCGCCGAAGGTATGGCGGGCGCGATTGCCAAAGCGCAATCCTTGGTGGACGCTCATCCAGACACTTATTTTATGCCGCGCCAGTTCGACAATGAGGCAAATCCCGAAGTCCACCGCAAAACAACCGCCGAGGAAATTTGGAACGATACCGACGGTAAAGTCGATGTCTTCGTTGCCGGCGTCGGCACGGGCGGTACGATTACCGGCGTGGGCGAAGTGTTGAAAAAATACAAACCCGAAATTGAAGTGTGTGCCGTCGAAGCTGGCGCTTCCCCCGTATTGAGCGGCGGCGAAAAAGGTCCGCACCCGATTCAAGGTATCGGCGCAGGTTTTATTCCGACCGTTTTGAATACCAAAATCTACGACAGCATTGCCAAAGTGCCGAACGAAGCGGCTTTTGAAACCGCCCGTGCAATGGCGGAAAAAGAAGGCATTTTGGCGGGCATTTCTTCCGGTGCGGCGGTTTGGAGCGCGTTGCAGCTTGCCAAACAGCCTGAAAACGAAGGCAAGCTGATAGTCGTGCTGCTGCCTTCTTATGGCGAACGCTATCTTTCTACGCCACTTTTTGCAGATTTGGCATAA

cDNA: SEQ ID NO: 50

TTATGCCAAATCTGCAAAAAGTGGCGTAGAAAGATAGCGTTCGCCATAAGAAGGCAGCAGCACGACTATCAGCTTGCCTTCGTTTTCAGGCTGTTTGGCAAGCTGCAACGCGCTCCAAACCGCCGCACCGGAAGAAATGCCCGCCAAAATGCCTTCTTTTTCCGCCATTGCACGGGCGGTTTCAAAAGCCGCTTCGTTCGGCACTTTGGCAATGCTGTCGTAGATTTTGGTATTCAAAACGGTCGGAATAAAACCTGCGCCGATACCTTGAATCGGGTGCGGACCTTTTTCGCCGCCGCTCAATACGGGGGAAGCGCCAGCTTCGACGGCACACACTTCAATTTCGGGTTTGTATTTTTTCAACACTTCGCCCACGCCGGTAATCGTACCGCCCGTGCCGACGCCGGCAACGAAGACATCGACTTTACCGTCGGTATCGTTCCAAATTTCCTCGGCGGTTGTTTTGCGGTGGACTTCGGGATTTGCCTCATTGTCGAACTGGCGCGGCATAAAATAAGTGTCTGGATGAGCGTCCACCAAGGATTGCGCTTTGGCAATCGCGCCCGCCATACCTTCGGCGGCGGGGGTTAGAATCAGTTCCGCGCCAAACGTGCGCAACAGCATTTTGCGCTCTTTGCTCATGCTTTCCGGCATGGTAATCGCCAGTTTGTAGCCGCGTGCGGCACATACCATTGCCAAACCGATACCCGTATTGCCGCTGGTTGCTTCGACAATGACGGTGTTTTTGTTGATTTTTCCCGCTTTTTCGGCGGCCTCGATCATTGCTTCGGCAATGCGGTCTTTGACGCTGCTGCCCGGATTAAAAAATTCCAGTTTCACGGCAACCTCTGCCTTCAAACCTTTGGTCAAACGGTTCAGTTTGACCAAAGGCGTGTTGCCGATCAATTCGGTGATGCTGTTTGCAATTTTCAT

RNA: SEQ ID NO 51

AUGAAAAUUGCAAACAGCAUCACCGAAUUGAUCGGCAACACGCCUUUGGUCAAACUGAACCGUUUGACCAAAGGUUUGAAGGCAGAGGUUGCCGUGAAACUGGAAUUUUUUAAUCCGGGCAGCAGCGUCAAAGACCGCAUUGCCGAAGCAAUGAUCGAGGCCGCCGAAAAAGCGGGAAAAAUCAACAAAAACACCGUCAUUGUCGAAGCAACCAGCGGCAAUACGGGUAUCGGUUUGGCAAUGGUAUGUGCCGCACGCGGCUACAAACUGGCGAUUACCAUGCCGGAAAGCAUGAGCAAAGAGCGCAAAAUGCUGUUGCGCACGUUUGGCGCGGAACUGAUUCUAACCCCCGCCGCCGAAGGUAUGGCGGGCGCGAUUGCCAAAGCGCAAUCCUUGGUGGACGCUCAUCCAGACACUUAUUUUAUGCCGCGCCAGUUCGACAAUGAGGCAAAUCCCGAAGUCCACCGCAAAACAACCGCCGAGGAAAUUUGGAACGAUACCGACGGUAAAGUCGAUGUCUUCGUUGCCGGCGUCGGCACGGGCGGUACGAUUACCGGCGUGGGCGAAGUGUUGAAAAAAUACAAACCCGAAAUUGAAGUGUGUGCCGUCGAAGCUGGCGCUUCCCCCGUAUUGAGCGGCGGCGAAAAAGGUCCGCACCCGAUUCAAGGUAUCGGCGCAGGUUUUAUUCCGACCGUUUUGAAUACCAAAAUCUACGACAGCAUUGCCAAAGUGCCGAACGAAGCGGCUUUUGAAACCGCCCGUGCAAUGGCGGAAAAAGAAGGCAUUUUGGCGGGCAUUUCUUCCGGUGCGGCGGUUUGGAGCGCGUUGCAGCUUGCCAAACAGCCUGAAAACGAAGGCAAGCUGAUAGUCGUGCUGCUGCCUUCUUAUGGCGAACGCUAUCUUUCUACGCCACUUUUUGCAGAUUUGGCA UAA

9. Ngo0592: Nc_002946.2:578772-580085

DNA (+ strand): SEQ ID NO: 52

ATGATGAGCGTAACTGTTGAAATTTTAGAAAATCTGGAACGCAAAGTAGTGTTGTCCCTGCCTTGGTCCGAAATCAACGCAGAAACCGATAAAAAACTGAAACAAACCCAACGCCGTGCAAAAATCGACGGTTTCCGTCCGGGTAAAGCACCTTTAAAAATGATTGCCCAAATGTACGGTGCGAGCGCGCAAAACGACGTGATCAACGAGCTGGTGCAACGCCGCTTCTACGATGTTGCCGTTGCCCAAGAGTTGAAAGTGGCAGGCTATCCACGTTTTGAAGGCGTTGAAGAACAAGACGATAAAGAGTCTTTCAAAGTTGCCGCCATTTTTGAAGTGTTCCCCGAAGTCGTTATCGGCGATTTGTCTGCACAAGAGGTCGAAAAAGTAACCGCTTCCGTCGGCGATGCCGAAGTCGACCAAACTGTAGAAATCCTGCGTAAACAACGTACCCGCTTCAACCATGTTGACCGCGAAGCCCGAAACGGCGACCGCGTCATCATCGACTTTGAAGGCAAAATCGACGGCGAACCTTTTGCCGGCGGCACATCCAAAAACTACGCCTTCGTATTGGGCGCAGGTCAAATGCTGCCTGAATTTGAAGCCGGCGTAGTCGGCATGAAAGCGGGCGAAAGTAAAGACGTTACCGTCAACTTCCCTGAAGAATACCACGGCAAAGATGTTGCCGGTAAAACTGCCGTGTTCACCATTACGCTGAACAATGTTTCCGAGCCCACTCTGCCTGAAGTCGATGCAGATTTTGCAAAAGCCTTGGGTATTGCGGATGGCGATGTTGCCAAAATGCGTGAAGAAGTGAAGAAAAACGTAAGCCGCGAAGTGGAACGCCGCGTGAACGAACAAACCAAAGAATCCGTAATGAACGCGCTGATTAAAGCCGTAGAGTTGAAAGTTCCGGTTGCTTTGGTCAATGAAGAAGCCGCCCGCCTGGCAAACGAAATGAAACAAAACTTCGTTAACCAAGGTATGACCGATGCCGCGAACTTGGATTTGCCTTTGGATATGTTCAAAGAACAAGCCGAACGTCGCGTATCTTTGGGTCTGATTTTGGCCAAACTGGTTGACGAAAACAAACTGGAACCGACTGAAGGGCAAATCAAAGCCGTTGTCGCCAACTTCGCAGAAAGCTACGAAGATCCTCAAGAAGTGATTGACTGGTACTACGCAGATACTTCCCGCCTGCAAGCCCCGACTTCTTTGGCAGTAGAAAGCAACGTTGTTGATTTCGTTTTGGGCAAAGCCAAAGTAAACAAAAAAGCTTTGTCTTTTGACGAAGTGATGG GCGCGCAAGCCTGA

cDNA: SEQ ID NO: 53

TCAGGCTTGCGCGCCCATCACTTCGTCAAAAGACAAAGCTTTTTTGTTTACTTTGGCTTTGCCCAAAACGAAATCAACAACGTTGCTTTCTACTGCCAAAGAAGTCGGGGCTTGCAGGCGGGAAGTATCTGCGTAGTACCAGTCAATCACTTCTTGAGGATCTTCGTAGCTTTCTGCGAAGTTGGCGACAACGGCTTTGATTTGCCCTTCAGTCGGTTCCAGTTTGTTTTCGTCAACCAGTTTGGCCAAAATCAGACCCAAAGATACGCGACGTTCGGCTTGTTCTTTGAACATATCCAAAGGCAAATCCAAGTTCGCGGCATCGGTCATACCTTGGTTAACGAAGTTTTGTTTCATTTCGTTTGCCAGGCGGGCGGCTTCTTCATTGACCAAAGCAACCGGAACTTTCAACTCTACGGCTTTAATCAGCGCGTTCATTACGGATTCTTTGGTTTGTTCGTTCACGCGGCGTTCCACTTCGCGGCTTACGTTTTTCTTCACTTCTTCACGCATTTTGGCAACATCGCCATCCGCAATACCCAAGGCTTTTGCAAAATCTGCATCGACTTCAGGCAGAGTGGGCTCGGAAACATTGTTCAGCGTAATGGTGAACACGGCAGTTTTACCGGCAACATCTTTGCCGTGGTATTCTTCAGGGAAGTTGACGGTAACGTCTTTACTTTCGCCCGCTTTCATGCCGACTACGCCGGCTTCAAATTCAGGCAGCATTTGACCTGCGCCCAATACGAAGGCGTAGTTTTTGGATGTGCCGCCGGCAAAAGGTTCGCCGTCGATTTTGCCTTCAAAGTCGATGATGACGCGGTCGCCGTTTCGGGCTTCGCGGTCAACATGGTTGAAGCGGGTACGTTGTTTACGCAGGATTTCTACAGTTTGGTCGACTTCGGCATCGCCGACGGAAGCGGTTACTTTTTCGACCTCTTGTGCAGACAAATCGCCGATAACGACTTCGGGGAACACTTCAAAAATGGCGGCAACTTTGAAAGACTCTTTATCGTCTTGTTCTTCAACGCCTTCAAAACGTGGATAGCCTGCCACTTTCAACTCTTGGGCAACGGCAACATCGTAGAAGCGGCGTTGCACCAGCTCGTTGATCACGTCGTTTTGCGCGCTCGCACCGTACATTTGGGCAATCATTTTTAAAGGTGCTTTACCCGGACGGAAACCGTCGATTTTTGCACGGCGTTGGGTTTGTTTCAGTTTTTTATCGGTTTCTGCGTTGATTTCGGACCAAGGCAGGGACAACACTACTTTGCGTTCCAGATTTTCTAAAATTTCAACAGTTACGCTCATCAT

RNA: SEQ ID NO: 54

AUGAUGAGCGUAACUGUUGAAAUUUUAGAAAAUCUGGAACGCAAAGUAGUGUUGUCCCUGCCUUGGUCCGAAAUCAACGCAGAAACCGAUAAAAAACUGAAACAAACCCAACGCCGUGCAAAAAUCGACGGUUUCCGUCCGGGUAAAGCACCUUUAAAAAUGAUUGCCCAAAUGUACGGUGCGAGCGCGCAAAACGACGUGAUCAACGAGCUGGUGCAACGCCGCUUCUACGAUGUUGCCGUUGCCCAAGAGUUGAAAGUGGCAGGCUAUCCACGUUUUGAAGGCGUUGAAGAACAAGACGAUAAAGAGUCUUUCAAAGUUGCCGCCAUUUUUGAAGUGUUCCCCGAAGUCGUUAUCGGCGAUUUGUCUGCACAAGAGGUCGAAAAAGUAACCGCUUCCGUCGGCGAUGCCGAAGUCGACCAAACUGUAGAAAUCCUGCGUAAACAACGUACCCGCUUCAACCAUGUUGACCGCGAAGCCCGAAACGGCGACCGCGUCAUCAUCGACUUUGAAGGCAAAAUCGACGGCGAACCUUUUGCCGGCGGCACAUCCAAAAACUACGCCUUCGUAUUGGGCGCAGGUCAAAUGCUGCCUGAAUUUGAAGCCGGCGUAGUCGGCAUGAAAGCGGGCGAAAGUAAAGACGUUACCGUCAACUUCCCUGAAGAAUACCACGGCAAAGAUGUUGCCGGUAAAACUGCCGUGUUCACCAUUACGCUGAACAAUGUUUCCGAGCCCACUCUGCCUGAAGUCGAUGCAGAUUUUGCAAAAGCCUUGGGUAUUGCGGAUGGCGAUGUUGCCAAAAUGCGUGAAGAAGUGAAGAAAAACGUAAGCCGCGAAGUGGAACGCCGCGUGAACGAACAAACCAAAGAAUCCGUAAUGAACGCGCUGAUUAAAGCCGUAGAGUUGAAAGUUCCGGUUGCUUUGGUCAAUGAAGAAGCCGCCCGCCUGGCAAACGAAAUGAAACAAAACUUCGUUAACCAAGGUAUGACCGAUGCCGCGAACUUGGAUUUGCCUUUGGAUAUGUUCAAAGAACAAGCCGAACGUCGCGUAUCUUUGGGUCUGAUUUUGGCCAAACUGGUUGACGAAAACAAACUGGAACCGACUGAAGGGCAAAUCAAAGCCGUUGUCGCCAACUUCGCAGAAAGCUACGAAGAUCCUCAAGAAGUGAUUGACUGGUACUACGCAGAUACUUCCCGCCUGCAAGCCCCGACUUCUUUGGCAGUAGAAAGCAACGUUGUUGAUUUCGUUUUGGGCAAAGCCAAAGUAAACAAAAAAGCUUUGUCUUUUGACGAAGUGAUGGGCGCGCAAG CCUGA

10. Ngo0593: Nc_002946.2:580181-580795

DNA (+ strand): SEQ ID NO: 55

ATGTCTTTTGATAACCATCTTGTCCCTACCGTTATCGAGCAGAGCGGTCGCGGTGAGCGTGCATTCGATATCTATTCCCGGCTTTTGAAAGAGCGCATCGTATTTCTGGTTGGCCCGGTAACCGATGAGTCTGCTAATCTGGTGGTCGCCCAACTGTTGTTTTTGGAAAGTGAGAATCCGGATAAGGATATTTTCTTCTACATCAATTCCCCCGGCGGCTCGGTAACGGCCGGTATGTCGATTTACGACACGATGAATTTCATCAAGCCCGATGTATCGACTTTGTGCTTGGGGCAGGCGGCAAGTATGGGCGCGTTCTTATTGTCGGCAGGCGAGAAAGGCAAACGTTTCGCCCTGCCCAACAGCCGGATTATGATTCACCAGCCTTTAATCAGCGGCGGCTTGGGCGGTCAGGCATCCGACATTGAAATCCACGCACGCGAGTTGTTGAAAATCAAAGAAAAACTCAACCGCCTGATGGCGAAACATTGCGGCCGCGATTTGGCAGATTTGGAGCGCGACACCGACCGTGATAATTTCATGTCTGCCGAAGAAGCAAAAGAATATGGTTTGATCGACCAAGTTTTGGAAAACCGCGCTTCTTTGCGGCTTTAA

cDNA: SEQ ID NO: 56

TTAAAGCCGCAAAGAAGCGCGGTTTTCCAAAACTTGGTCGATCAAACCATATTCTTTTGCTTCTTCGGCAGACATGAAATTATCACGGTCGGTGTCGCGCTCCAAATCTGCCAAATCGCGGCCGCAATGTTTCGCCATCAGGCGGTTGAGTTTTTCTTTGATTTTCAACAACTCGCGTGCGTGGATTTCAATGTCGGATGCCTGACCGCCCAAGCCGCCGCTGATTAAAGGCTGGTGAATCATAATCCGGCTGTTGGGCAGGGCGAAACGTTTGCCTTTCTCGCCTGCCGACAATAAGAACGCGCCCATACTTGCCGCCTGCCCCAAGCACAAAGTCGATACATCGGGCTTGATGAAATTCATCGTGTCGTAAATCGACATACCGGCCGTTACCGAGCCGCCGGGGGAATTGATGTAGAAGAAAATATCCTTATCCGGATTCTCACTTTCCAAAAACAACAGTTGGGCGACCACCAGATTAGCAGACTCATCGGTTACCGGGCCAACCAGAAATACGATGCGCTCTTTCAAAAGCCGGGAATAGATATCGAATGCACGCTCACCGCGACCGCTCTGCTCGATAACGGTAGGGACAAGATGGTTATCAAAAGACAT

RNA: SEQ ID NO: 57

AUGUCUUUUGAUAACCAUCUUGUCCCUACCGUUAUCGAGCAGAGCGGUCGCGGUGAGCGUGCAUUCGAUAUCUAUUCCCGGCUUUUGAAAGAGCGCAUCGUAUUUCUGGUUGGCCCGGUAACCGAUGAGUCUGCUAAUCUGGUGGUCGCCCAACUGUUGUUUUUGGAAAGUGAGAAUCCGGAUAAGGAUAUUUUCUUCUACAUCAAUUCCCCCGGCGGCUCGGUAACGGCCGGUAUGUCGAUUUACGACACGAUGAAUUUCAUCAAGCCCGAUGUAUCGACUUUGUGCUUGGGGCAGGCGGCAAGUAUGGGCGCGUUCUUAUUGUCGGCAGGCGAGAAAGGCAAACGUUUCGCCCUGCCCAACAGCCGGAUUAUGAUUCACCAGCCUUUAAUCAGCGGCGGCUUGGGCGGUCAGGCAUCCGACAUUGAAAUCCACGCACGCGAGUUGUUGAAAAUCAAAGAAAAACUCAACCGCCUGAUGGCGAAACAUUGCGGCCGCGAUUUGGCAGAUUUGGAGCGCGACACCGACCGUGAUAAUUUCAUGUCUGCCGAAGAAGCAAAAGAAUAUGGUUUGAUCGACCAAGUUUUGGAAAACCGCGC UUCUUUGCGGCUUUAA

11. NGO0604: NC_002946.2:c591006-589321

DNA (- strand): SEQ ID NO: 58

ATGTCTATGGAAAATTTTGCTCAGCTGTTGGAAGAAAGCTTTACCCTGCAAGAAATGACCCGGGTGAGGTGATTACCGCTGAAGTAGTGGCAATCGACCAAAACTTCGTTACCGTAAACGCAGGTCTGAAATCAGAATCCCTGATCGATGTAGCTGAATTCAAAAACGCTCAAGGCGAAATTGAAGTTAAAGTCGGCGACTTCGTTACCGTTACCATCGAATCCGTCGAAAACGGCTTCGGCGAAACCAAACTGTCCCGCGAAAAAGCCAAACGCGCAGCCGATTGGATCGCTTTGGAAGAAGCCATGGAAAACGGCAACATCCTGTCCGGCATCATCAACGGTAAAGTCAAAGGCGGCCTGACCGTTATGATCAGCAGCATCCGCGCATTCCTGCCGGGTTCTTTGGTCGACGTACGTCCCGTTAAAGACACTTCCCATTTTGAAGGCAAAGAGATCGAATTCAAAGTGATCAAACTGGACAAAAAACGCAACAACGTCGTTGTTTCCCGCCGCGCCGTTTTGGAAGCCACTTTGGGTGAAGAACGCAAAGCCCTGCTGGAAAACCTGCAAGAAGGCTCCGTCATCAAAGGCATCGTCAAAAATATCACCGACTACGGCGCATTCGTTGACCTGGGCGGCATCGACGGCCTGCTGCACATCACCGATTTGGCATGGCGTCGCGTGAAACACCCGAGCGAAGTCTTGGAAGTCGGTCAGGAAGTTGAAGCCAAAGTATTGAAATTCGACCAAGAAAAACAACGTGTTTCCTTGGGTATGAAACAACTGGGCGAAGATCCTTGGAGCGGTCTGACCCGCCGTTATCCGCAAGCCACCCGCCTGTTCGGCAAAGTATCCAACCTGACCGACTACGGCGCATTCGTCGAAATCGAACAAGGCATCGAAGGTTTGGTACACGTCTCCGAAATGGACTGGACCAACAAAAACGTACACCCGAGCAAAGTCGTACAACTGGGTGACGAAGTCGAAGTCATGATTTTGGAAATCGACGAAGGCCGCCGCCGTATCTCTTTGGGTATGAAACAATGCCAAGCCAATCCTTGGGAAGAATTTGCCGCCAACCACAACAAAGGAGACAAAATCTCCGGTGCGGTTAAATCCATTACCGATTTCGGCGTATTCGTCGGCCTGCCCGGCGGCATCGACGGTCTGGTTCACCTGTCCGACCTGTCTTGGACCGAATCCGGCGAAGAAGCCGTACGCAAATACAAAAAAGGAGAAGAAGTCGAAGCCGTCGTATTGGCAATCGATGTGGAAAAAGAACGCATCTCCTTGGGTATCAAACAACTGGAAGGCGATCCTTTCGGCAACTTCATCAGCGTGAACGACAAAGGTTCTTTGGTTAAAGGTTCCGTGAAATCTGTTGACGCCAAAGGCGCTGTTATCGCCCTGTCTGACGAAGTAGAAGGCTACCTGCCTGCTTCCGAATTTGCAGCCGACCGCGTTGAAGACTTGACCACCAAACTGAAAGAAGGCGACGAAGTTGAAGCCGTCATCGTTACCGTTGACCGCAAAAACCGCAGCATCAAACTTTCCGTTAAAGCCAAAGATGCCAAAGAAAGCCGCGAAGCACTGAACTCCGTCAATGCCGCCGCCAATGCGAATGCCGGTACCACCAGCTTGGGCGACCTGCTGAAAGCCAAACTCTCCGGCGAACAAGAATAA

cDNA: SEQ ID NO: 59

TTATTCTTGTTCGCCGGAGAGTTTGGCTTTCAGCAGGTCGCCCAAGCTGGTGGTACCGGCATTCGCATTGGCGGCGGCATTGACGGAGTTCAGTGCTTCGCGGCTTTCTTTGGCATCTTTGGCTTTAACGGAAAGTTTGATGCTGCGGTTTTTGCGGTCAACGGTAACGATGACGGCTTCAACTTCGTCGCCTTCTTTCAGTTTGGTGGTCAAGTCTTCAACGCGGTCGGCTGCAAATTCGGAAGCAGGCAGGTAGCCTTCTACTTCGTCAGACAGGGCGATAACAGCGCCTTTGGCGTCAACAGATTTCACGGAACCTTTAACCAAAGAACCTTTGTCGTTCACGCTGATGAAGTTGCCGAAAGGATCGCCTTCCAGTTGTTTGATACCCAAGGAGATGCGTTCTTTTTCCACATCGATTGCCAATACGACGGCTTCGACTTCTTCTCCTTTTTTGTATTTGCGTACGGCTTCTTCGCCGGATTCGGTCCAAGACAGGTCGGACAGGTGAACCAGACCGTCGATGCCGCCGGGCAGGCCGACGAATACGCCGAAATCGGTAATGGATTTAACCGCACCGGAGATTTTGTCTCCTTTGTTGTGGTTGGCGGCAAATTCTTCCCAAGGATTGGCTTGGCATTGTTTCATACCCAAAGAGATACGGCGGCGGCCTTCGTCGATTTCCAAAATCATGACTTCGACTTCGTCACCCAGTTGTACGACTTTGCTCGGGTGTACGTTTTTGTTGGTCCAGTCCATTTCGGAGACGTGTACCAAACCTTCGATGCCTTGTTCGATTTCGACGAATGCGCCGTAGTCGGTCAGGTTGGATACTTTGCCGAACAGGCGGGTGGCTTGCGGATAACGGCGGGTCAGACCGCTCCAAGGATCTTCGCCCAGTTGTTTCATACCCAAGGAAACACGTTGTTTTTCTTGGTCGAATTTCAATACTTTGGCTTCAACTTCCTGACCGACTTCCAAGACTTCGCTCGGGTGTTTCACGCGACGCCATGCCAAATCGGTGATGTGCAGCAGGCCGTCGATGCCGCCCAGGTCAACGAATGCGCCGTAGTCGGTGATATTTTTGACGATGCCTTTGATGACGGAGCCTTCTTGCAGGTTTTCCAGCAGGGCTTTGCGTTCTTCACCCAAAGTGGCTTCCAAAACGGCGCGGCGGGAAACAACGACGTTGTTGCGTTTTTTGTCCAGTTTGATCACTTTGAATTCGATCTCTTTGCCTTCAAAATGGGAAGTGTCTTTAACGGGACGTACGTCGACCAAAGAACCCGGCAGGAATGCGCGGATGCTGCTGATCATAACGGTCAGGCCGCCTTTGACTTTACCGTTGATGATGCCGGACAGGATGTTGCCGTTTTCCATGGCTTCTTCCAAAGCGATCCAATCGGCTGCGCGTTTGGCTTTTTCGCGGGACAGTTTGGTTTCGCCGAAGCCGTTTTCGACGGATTCGATGGTAACGGTAACGAAGTCGCCGACTTTAACTTCAATTTCGCCTTGAGCGTTTTTGAATTCAGCTACATCGATCAGGGATTCTGATTTCAGACCTGCGTTTACGGTAACGAAGTTTTGGTCGATTGCCACTACTTCAGCGGTAATCACCTCACCCGGGTTCATTTCTTGCAGGGTAAAGCTTTCTTCCAACAGCTGAGCAAAATTTTCCATAGACAT

RNA: SEQ ID NO: 60

AUGUCUAUGGAAAAUUUUGCUCAGCUGUUGGAAGAAAGCUUUACCCUGCAAGAAAUGAACCCGGGUGAGGUGAUUACCGCUGAAGUAGUGGCAAUCGACCAAAACUUCGUUACCGUAAACGCAGGUCUGAAAUCAGAAUCCCUGAUCGAUGUAGCUGAAUUCAAAAACGCUCAAGGCGAAAUUGAAGUUAAAGUCGGCGACUUCGUUACCGUUACCAUCGAAUCCGUCGAAAACGGCUUCGGCGAAACCAAACUGUCCCGCGAAAAAGCCAAACGCGCAGCCGAUUGGAUCGCUUUGGAAGAAGCCAUGGAAAACGGCAACAUCCUGUCCGGCAUCAUCAACGGUAAAGUCAAAGGCGGCCUGACCGUUAUGAUCAGCAGCAUCCGCGCAUUCCUGCCGGGUUCUUUGGUCGACGUACGUCCCGUUAAAGACACUUCCCAUUUUGAAGGCAAAGAGAUCGAAUUCAAAGUGAUCAAACUGGACAAAAAACGCAACAACGUCGUUGUUUCCCGCCGCGCCGUUUUGGAAGCCACUUUGGGUGAAGAACGCAAAGCCCUGCUGGAAAACCUGCAAGAAGGCUCCGUCAUCAAAGGCAUCGUCAAAAAUAUCACCGACUACGGCGCAUUCGUUGACCUGGGCGGCAUCGACGGCCUGCUGCACAUCACCGAUUUGGCAUGGCGUCGCGUGAAACACCCGAGCGAAGUCUUGGAAGUCGGUCAGGAAGUUGAAGCCAAAGUAUUGAAAUUCGACCAAGAAAAACAACGUGUUUCCUUGGGUAUGAAACAACUGGGCGAAGAUCCUUGGAGCGGUCUGACCCGCCGUUAUCCGCAAGCCACCCGCCUGUUCGGCAAAGUAUCCAACCUGACCGACUACGGCGCAUUCGUCGAAAUCGAACAAGGCAUCGAAGGUUUGGUACACGUCUCCGAAAUGGACUGGACCAACAAAAACGUACACCCGAGCAAAGUCGUACAACUGGGUGACGAAGUCGAAGUCAUGAUUUUGGAAAUCGACGAAGGCCGCCGCCGUAUCUCUUUGGGUAUGAAACAAUGCCAAGCCAAUCCUUGGGAAGAAUUUGCCGCCAACCACAACAAAGGAGACAAAAUCUCCGGUGCGGUUAAAUCCAUUACCGAUUUCGGCGUAUUCGUCGGCCUGCCCGGCGGCAUCGACGGUCUGGUUCACCUGUCCGACCUGUCUUGGACCGAAUCCGGCGAAGAAGCCGUACGCAAAUACAAAAAAGGAGAAGAAGUCGAAGCCGUCGUAUUGGCAAUCGAUGUGGAAAAAGAACGCAUCUCCUUGGGUAUCAAACAACUGGAAGGCGAUCCUUUCGGCAACUUCAUCAGCGUGAACGACAAAGGUUCUUUGGUUAAAGGUUCCGUGAAAUCUGUUGACGCCAAAGGCGCUGUUAUCGCCCUGUCUGACGAAGUAGAAGGCUACCUGCCUGCUUCCGAAUUUGCAGCCGACCGCGUUGAAGACUUGACCACCAAACUGAAAGAAGGCGACGAAGUUGAAGCCGUCAUCGUUACCGUUGACCGCAAAAACCGCAGCAUCAAACUUUCCGUUAAAGCCAAAGAUGCCAAAGAAAGCCGCGAAGCACUGAACUCCGUCAAUGCCGCCGCCAAUGCGAAUGCCGGUACCACCAGCUUGGGCGACCUGCUGAAAGCCAAACUCUCCGGCGAACAAGAAUAA

12. NGO0618: NC_002946.2:c606708-606268

DNA (- strand): SEQ ID NO: 61

ATGATGCAGACTTTCCGAAAAATCAGCCTGTATGCCGCAACCTTGTGGCTCGGTATGCAGATTATGGCAGGTTATATCGCCGCACCGGTGCTGTTCAAAATGCTGCCCAAAATGCAGGCGGGCGAAATTGCCGGCGTATTGTTCGACATCCTCTCTTGGAGCGGGCTTGCCGTTTGGGGCACGGTACTGGCTGCCGCCTTTGCCGCCCTAACCCGGCGGCAAACCGCCCTGCTGCTTTTTTTATTGTCCGCCCTTGCCGCCAACCAATTTTTGGTTACACCCGTTATCGAGGCACTGAAATACGGGCATGAAAATTGGCTGTTGTCGGTTGCAGGCGGATCCTTCGGAATGTGGCACGGTATTTCCAGCATGACTTTCATGGCAACCGCCCTACTTTCAGCAGTTTTAAGTTGGCGGCTTTCCGGCAAAGAGGCCGTCTGA

cDNA: SEQ ID NO: 62

TCAGACGGCCTCTTTGCCGGAAAGCCGCCAACTTAAAACTGCTGAAAGTAGGGCGGTTGCCATGAAAGTCATGCTGGAAATACCGTGCCACATTCCGAAGGATCCGCCTGCAACCGACAACAGCCAATTTTCATGCCCGTATTTCAGTGCCTCGATAACGGGTGTAACCAAAAATTGGTTGGCGGCAAGGGCGGACAATAAAAAAAGCAGCAGGGCGGTTTGCCGCCGGGTTAGGGCGGCAAAGGCGGCAGCCAGTACCGTGCCCCAAACGGCAAGCCCGCTCCAAGAGAGGATGTCGAACAATACGCCGGCAATTTCGCCCGCCTGCATTTTGGGCAGCATTTTGAACAGCACCGGTGCGGCGATATAACCTGCCATAATCTGCATACCGAGCCACAAGGTTGCGGCATACAGGCTGATTTTTCGGAAAGTCTGCATCAT

RNA: SEQ ID NO: 63

AUGAUGCAGACUUUCCGAAAAAUCAGCCUGUAUGCCGCAACCUUGUGGCUCGGUAUGCAGAUUAUGGCAGGUUAUAUCGCCGCACCGGUGCUGUUCAAAAUGCUGCCCAAAAUGCAGGCGGGCGAAAUUGCCGGCGUAUUGUUCGACAUCCUCUCUUGGAGCGGGCUUGCCGUUUGGGGCACGGUACUGGCUGCCGCCUUUGCCGCCCUAACCCGGCGGCAAACCGCCCUGCUGCUUUUUUUAUUGUCCGCCCUUGCCGCCAACCAAUUUUUGGUUACACCCGUUAUCGAGGCACUGAAAUACGGGCAUGAAAAUUGGCUGUUGUCGGUUGCAGGCGGAUCCUUCGGAAUGUGGCACGGUAUUUCCAGCAUGACUUUCAUGGCAACCGCCCUACUUUCAGCAGUUUUAAGUUGGCGGCUUUCCGGCAAAGAGGCCGU CUGA

13. NGO0619: NC_002946.2:c607565-606723

DNA (- strand): SEQ ID NO: 64

ATGGATATTAAAATCAACGACATCACCCTCGGCAACAATTCGCCTTTCGTCCTATTCGGCGGCATCAACGTTTTAGAAGATTTGGATTCCACCCTCCAAACCTGTGCGCATTACGTCGAAGTTACCCGCAAACTGGGCATCCCCTATATCTTTAAAGCCTCTTTCGACAAGGCAAACCGCTCGTCTATCCATTCCTATCGCGGCGTAGGCTTGGAAGAAGGCTTAAAGATTTTTGAAAAAGTCAAAGCAGAGTTCGGCATCCCCGTCATTACCGACGTACACGAACCCCATCAATGCCAACCCGTCGCCGAAGTGTGCGATGTCATCCAGCTTCCCGCCTTTCTTGCGCGGCAGACCGATTTGGTGGCCGCAATGGCGGAAACGGGCAATGTTATCAACATCAAAAAACCCCAGTTCCTCAGCCCTTCGCAAATGAAAAACATCGTGGAAAAATTCCGCGAAGCCGGCAACGGGAAGCTGATTTTATGCGAACGCGGCAGCAGCTTCGGCTACGACAACCTCGTTGTCGATATGCTCGGTTTCGGCGTGATGAAACAAACCTGCGGCAACCTGCCGGTTATTTTCGACGTTACCCATTCCCTGCAAACCCGCGATGCCGGTTCTGCCGCATCCGGCGGTCGTCGCGCACAGGCTTTGGATTTGGCACTTGCAGGCATGGCAACCCGCCTTGCCGGCCTGTTCCTCGAATCGCACCCCGATCCGAAACTGGCAAAATGCGACGGCCCCAGCGCGCTGCCGCTACACCTTTTAGAAAATTTTTTAATCCGCATCAAAGCATTGGACGATTTAATCAAATCACAACCGATTTTAACAATCGAGTAA

cDNA: SEQ ID NO: 65

TTACTCGATTGTTAAAATCGGTTGTGATTTGATTAAATCGTCCAATGCTTTGATGCGGATTAAAAAATTTTCTAAAAGGTGTAGCGGCAGCGCGCTGGGGCCGTCGCATTTTGCCAGTTTCGGATCGGGGTGCGATTCGAGGAACAGGCCGGCAAGGCGGGTTGCCATGCCTGCAAGTGCCAAATCCAAAGCCTGTGCGCGACGACCGCCGGATGCGGCAGAACCGGCATCGCGGGTTTGCAGGGAATGGGTAACGTCGAAAATAACCGGCAGGTTGCCGCAGGTTTGTTTCATCACGCCGAAACCGAGCATATCGACAACGAGGTTGTCGTAGCCGAAGCTGCTGCCGCGTTCGCATAAAATCAGCTTCCCGTTGCCGGCTTCGCGGAATTTTTCCACGATGTTTTTCATTTGCGAAGGGCTGAGGAACTGGGGTTTTTTGATGTTGATAACATTGCCCGTTTCCGCCATTGCGGCCACCAAATCGGTCTGCCGCGCAAGAAAGGCGGGAAGCTGGATGACATCGCACACTTCGGCGACGGGTTGGCATTGATGGGGTTCGTGTACGTCGGTAATGACGGGGATGCCGAACTCTGCTTTGACTTTTTCAAAAATCTTTAAGCCTTCTTCCAAGCCTACGCCGCGATAGGAATGGATAGACGAGCGGTTTGCCTTGTCGAAAGAGGCTTTAAAGATATAGGGGATGCCCAGTTTGCGGGTAACTTCGACGTAATGCGCACAGGTTTGGAGGGTGGAATCCAAATCTTCTAAAACGTTGATGCCGCCGAATAGGACGAAAGGCGAATTGTTGCCGAGGGTGATGTCGTTGATTTTAATATCCAT

RNA: SEQ ID NO: 66

AUGGAUAUUAAAAUCAACGACAUCACCCUCGGCAACAAUUCGCCUUUCGUCCUAUUCGGCGGCAUCAACGUUUUAGAAGAUUUGGAUUCCACCCUCCAAACCUGUGCGCAUUACGUCGAAGUUACCCGCAAACUGGGCAUCCCCUAUAUCUUUAAAGCCUCUUUCGACAAGGCAAACCGCUCGUCUAUCCAUUCCUAUCGCGGCGUAGGCUUGGAAGAAGGCUUAAAGAUUUUUGAAAAAGUCAAAGCAGAGUUCGGCAUCCCCGUCAUUACCGACGUACACGAACCCCAUCAAUGCCAACCCGUCGCCGAAGUGUGCGAUGUCAUCCAGCUUCCCGCCUUUCUUGCGCGGCAGACCGAUUUGGUGGCCGCAAUGGCGGAAACGGGCAAUGUUAUCAACAUCAAAAAACCCCAGUUCCUCAGCCCUUCGCAAAUGAAAAACAUCGUGGAAAAAUUCCGCGAAGCCGGCAACGGGAAGCUGAUUUUAUGCGAACGCGGCAGCAGCUUCGGCUACGACAACCUCGUUGUCGAUAUGCUCGGUUUCGGCGUGAUGAAACAAACCUGCGGCAACCUGCCGGUUAUUUUCGACGUUACCCAUUCCCUGCAAACCCGCGAUGCCGGUUCUGCCGCAUCCGGCGGUCGUCGCGCACAGGCUUUGGAUUUGGCACUUGCAGGCAUGGCAACCCGCCUUGCCGGCCUGUUCCUCGAAUCGCACCCCGAUCCGAAACUGGCAAAAUGCGACGGCCCCAGCGCGCUGCCGCUACACCUUUUAGAAAAUUUUUUAAUCCGCAUCAAAGCAUUGGACGAUUUAAUCAAAUCACAACCGAUUUUAACAAUCGAGUAA

14. NGO0620: NC_002946.2:c607970-607587

DNA (- strand): SEQ ID NO: 67

ATGTTCCGTACTATACTTGGCGGAAAAATCCACCGCGCCACCGTAACCGAAGCCGATTTAAACTACGTCGGCAGCATTACCGTCGATCAAGACCTGTTAGACGCGGCAGGCATCTGCCCCAACGAAAAAGTCGCCATCGTCAACAACAACAACGGCGAACGTTTTGAAACCTATACCATTGCAGGGAAACGCGGCAGCGGCGTGATTTGCCTGAACGGTGCTGCAGCCAGGCTGGTACAGAAAGGCGACATCGTCATCATTATGTCTTATATCCAACTTTCCGAACCGGAAATCGCCGCACACGAACCCAAAGTCGTCTTAGTGGACGGAAACAATAAAATCCGCGACATCATCTCCTACGAGCCGCCGCACACCGTACTGTAA

cDNA: SEQ ID NO: 68

TTACAGTACGGTGTGCGGCGGCTCGTAGGAGATGATGTCGCGGATTTTATTGTTTCCGTCCACTAAGACGACTTTGGGTTCGTGTGCGGCGATTTCCGGTTCGGAAAGTTGGATATAAGACATAATGATGACGATGTCGCCTTTCTGTACCAGCCTGGCTGCAGCACCGTTCAGGCAAATCACGCCGCTGCCGCGTTTCCCTGCAATGGTATAGGTTTCAAAACGTTCGCCGTTGTTGTTGTTGACGATGGCGACTTTTTCGTTGGGGCAGATGCCTGCCGCGTCTAACAGGTCTTGATCGACGGTAATGCTGCCGACGTAGTTTAAATCGGCTTCGGTTACGGTGGCGCGGTGGATTTTTCCGCCAAGTATAGTACGGAACAT

RNA: SEQ ID NO: 69

AUGUUCCGUACUAUACUUGGCGGAAAAAUCCACCGCGCCACCGUAACCGAAGCCGAUUUAAACUACGUCGGCAGCAUUACCGUCGAUCAAGACCUGUUAGACGCGGCAGGCAUCUGCCCCAACGAAAAAGUCGCCAUCGUCAACAACAACAACGGCGAACGUUUUGAAACCUAUACCAUUGCAGGGAAACGCGGCAGCGGCGUGAUUUGCCUGAACGGUGCUGCAGCCAGGCUGGUACAGAAAGGCGACAUCGUCAUCAUUAUGUCUUAUAUCCAACUUUCCGAACCGGAAAUCGCCGCACACGAACCCAAAGUCGUCUUAGUGGACGGAAACAAUAAAAUCCGCGACAUCAUCUCCUACGAGCCGCCGCACACCGUACUGUAA   

15. Ngo0648: Nc_002946.2:638163-638717

DNA (+ strand): SEQ ID NO: 70

ATGAAAAAAATCATCGCCTCCGCGCTTATCGCAACATTCGCACTCACCGCCTGCCAAGACGACACGCAGGCGCGGCTCGAACGGCAGCAGAAACAGATTGAAGCCCTGCAACAGCAGCTCGCACAGCAGGCAGACGATACGGTTTACCAACTGACTCCCGAAGCAGTCAAAGACACCATTCCTGCCCAGGCGCAGGCAAACGGCAACAACGGTCAGCCCGTTACCGGCAAAGACGGGCAGCAGTATATTTACGACCAATCGACAGGAAGCTGGCTGCTGCAAAGCCTGATTGGCGCGGCGGCAGGCGCGTTTATCGGCAACGCGCTGGCAAACAAATTCACACGGGCGGGCAACCAAGACAGCCCCGTCGCCCGTCGCGCGCGTGCTGCCTACCATCAGTCCGCACGCCCCAATGCGCGCACCAGCAGGGATTTGAACACGCGCAGCCTCCGTGCAAAACAACAGGCGGCGCAGGCGCAGCGTTACCGCCCGACAACGCGCCCGCCCGTCAATTACCGCCGTCCCGCTATGCGCGGTTTCGGCAGAAGGCGGTAA

cDNA: SEQ ID NO: 71

TTACCGCCTTCTGCCGAAACCGCGCATAGCGGGACGGCGGTAATTGACGGGCGGGCGCGTTGTCGGGCGGTAACGCTGCGCCTGCGCCGCCTGTTGTTTTGCACGGAGGCTGCGCGTGTTCAAATCCCTGCTGGTGCGCGCATTGGGGCGTGCGGACTGATGGTAGGCAGCACGCGCGCGACGGGCGACGGGGCTGTCTTGGTTGCCCGCCCGTGTGAATTTGTTTGCCAGCGCGTTGCCGATAAACGCGCCTGCCGCCGCGCCAATCAGGCTTTGCAGCAGCCAGCTTCCTGTCGATTGGTCGTAAATATACTGCTGCCCGTCTTTGCCGGTAACGGGCTGACCGTTGTTGCCGTTTGCCTGCGCCTGGGCAGGAATGGTGTCTTTGACTGCTTCGGGAGTCAGTTGGTAAACCGTATCGTCTGCCTGCTGTGCGAGCTGCTGTTGCAGGGCTTCAATCTGTTTCTGCTGCCGTTCGAGCCGCGCCTGCGTGTCGTCTTGGCAGGCGGTGAGTGCGAATGTTGCGATAAGCGCGGAGGCGATGATTTTTTTCAT

RNA: SEQ ID NO: 72

AUGAAAAAAAUCAUCGCCUCCGCGCUUAUCGCAACAUUCGCACUCACCGCCUGCCAAGACGACACGCAGGCGCGGCUCGAACGGCAGCAGAAACAGAUUGAAGCCCUGCAACAGCAGCUCGCACAGCAGGCAGACGAUACGGUUUACCAACUGACUCCCGAAGCAGUCAAAGACACCAUUCCUGCCCAGGCGCAGGCAAACGGCAACAACGGUCAGCCCGUUACCGGCAAAGACGGGCAGCAGUAUAUUUACGACCAAUCGACAGGAAGCUGGCUGCUGCAAAGCCUGAUUGGCGCGGCGGCAGGCGCGUUUAUCGGCAACGCGCUGGCAAACAAAUUCACACGGGCGGGCAACCAAGACAGCCCCGUCGCCCGUCGCGCGCGUGCUGCCUACCAUCAGUCCGCACGCCCCAAUGCGCGCACCAGCAGGGAUUUGAACACGCGCAGCCUCCGUGCAAAACAACAGGCGGCGCAGGCGCAGCGUUACCGCCCGACAACGCGCCCGCCCGUCAAUUACCGCCGUCCCGCUAUGCGCGGUUUCGGCAGAAGG CGGUAA

16. Ngo1291: Nc_002946.2:1246814-1247542

DNA (+ strand): SEQ ID NO: 73

ATGGCAGGCCATAGCAAGTGGGCGAATATCCAGCATAAAAAAGCCCGTCAGGATGCCAAACGCGGCAAAATCTTCACCCGTTTAATCAAAGAAATCACCGTTGCGGCGCGTATGGGCGGCGGCGATCCCGGCGCAAATCCGCGCCTGCGTCTGGCTTTGGAAAAAGCAGCCGAAAACAATATGCCCAAAGACAATGTGCAACGCGCCATCGACAAAGGTACGGGTAACTTGGAAGGCGTGGAATACATCGAGTTGCGCTACGAAGGCTACGGCATCGGCGGCGCAGCTTTGATGGTGGACTGCCTGACCGACAACAAAACCCGCACCGTTGCGGACGTACGCCACGCATTTACCAAAAACGGCGGCAACTTGGGTACCGACGGCTGCGTGGCGTTCAACTTCGTGCATCAGGGCTATTTGGTATTCGAACCCGGCGTTGACGAAGACGAGCTGATGGAAGCGGCTTTGGAAGCCGGTGCGGAAGACGTGGTTACCAACGACGACGGTTCCATCGAAGTCATTACCGCGCCAAATGATTGGGCGGGCGTAAAATCCGCTTTGGAGGCGGCAGGTTACAAATCCGTTGACGGCGACGTTACGATGCGCGCCCAAAACGAAACCGAACTCTCCGGCGACGATGCCGTCAAAATGCAAAAACTGATTGACGCGCTGGAAGACTTGGACGACGTGCAAGACGTTTACACTTCCGCCGTATTGAATCTGGACTGA

cDNA: SEQ ID NO: 74

TCAGTCCAGATTCAATACGGCGGAAGTGTAAACGTCTTGCACGTCGTCCAAGTCTTCCAGCGCGTCAATCAGTTTTTGCATTTTGACGGCATCGTCGCCGGAGAGTTCGGTTTCGTTTTGGGCGCGCATCGTAACGTCGCCGTCAACGGATTTGTAACCTGCCGCCTCCAAAGCGGATTTTACGCCCGCCCAATCATTTGGCGCGGTAATGACTTCGATGGAACCGTCGTCGTTGGTAACCACGTCTTCCGCACCGGCTTCCAAAGCCGCTTCCATCAGCTCGTCTTCGTCAACGCCGGGTTCGAATACCAAATAGCCCTGATGCACGAAGTTGAACGCCACGCAGCCGTCGGTACCCAAGTTGCCGCCGTTTTTGGTAAATGCGTGGCGTACGTCCGCAACGGTGCGGGTTTTGTTGTCGGTCAGGCAGTCCACCATCAAAGCTGCGCCGCCGATGCCGTAGCCTTCGTAGCGCAACTCGATGTATTCCACGCCTTCCAAGTTACCCGTACCTTTGTCGATGGCGCGTTGCACATTGTCTTTGGGCATATTGTTTTCGGCTGCTTTTTCCAAAGCCAGACGCAGGCGCGGATTTGCGCCGGGATCGCCGCCGCCCATACGCGCCGCAACGGTGATTTCTTTGATTAAACGGGTGAAGATTTTGCCGCGTTTGGCATCCTGACGGGCTTTTTTATGCTGGATATTCGCCCACTTGCTATGGCCTGCCAT

RNA: SEQ ID NO: 75

AUGGCAGGCCAUAGCAAGUGGGCGAAUAUCCAGCAUAAAAAAGCCCGUCAGGAUGCCAAACGCGGCAAAAUCUUCACCCGUUUAAUCAAAGAAAUCACCGUUGCGGCGCGUAUGGGCGGCGGCGAUCCCGGCGCAAAUCCGCGCCUGCGUCUGGCUUUGGAAAAAGCAGCCGAAAACAAUAUGCCCAAAGACAAUGUGCAACGCGCCAUCGACAAAGGUACGGGUAACUUGGAAGGCGUGGAAUACAUCGAGUUGCGCUACGAAGGCUACGGCAUCGGCGGCGCAGCUUUGAUGGUGGACUGCCUGACCGACAACAAAACCCGCACCGUUGCGGACGUACGCCACGCAUUUACCAAAAACGGCGGCAACUUGGGUACCGACGGCUGCGUGGCGUUCAACUUCGUGCAUCAGGGCUAUUUGGUAUUCGAACCCGGCGUUGACGAAGACGAGCUGAUGGAAGCGGCUUUGGAAGCCGGUGCGGAAGACGUGGUUACCAACGACGACGGUUCCAUCGAAGUCAUUACCGCGCCAAAUGAUUGGGCGGGCGUAAAAUCCGCUUUGGAGGCGGCAGGUUACAAAUCCGUUGACGGCGACGUUACGAUGCGCGCCCAAAACGAAACCGAACUCUCCGGCGACGAUGCCGUCAAAAUGCAAAAACUGAUUGACGCGCUGGAAGACUUGGACGACGUGCAAGACGUUUACACUUCCGCCGUAUUGAAUCUGGACUGA

17. NGO1440: NC_002946.2:c1406345-1405167

DNA (- strand): SEQ ID NO: 76

ATGGCAAAAATGATGAAATGGGCGGCTGTTGCGGCGGTCGCGGCGGCAGCGGTTTGGGGCGGATGGTCTTATCTGAAGCCCGAACCGCAGGCTGCTTATATTACGGAAACGGTCAGGCGCGGCGATATCAGCCGGACGGTTTCCGCGACGGGCGAGATTTCGCCGTCCAACCTGGTATCGGTCGGCGCGCAGGCTTCGGGGCAGATTAAAAAGCTTTATGTCAAACTCGGGCAACAGGTCAAAAAGGGCGATTTGATTGCGGAAATCAATTCGACCACGCAGACCAACACGATCGATATGGAAAAATCCAAATTGGAAACGTATCAGGCGAAGCTGGTGTCGGCACAGATTGCATTGGGCAGCGCGGAGAAGAAATATAAGCGTCAGGCGGCGTTGTGGAAGGATGATGCGACCTCTAAAGAAGATTTGGAAAGCGCGCAGGATGCGCTTGCCGCCGCCAAAGCCAATGTTGCCGAGTTGAAGGCTTTAATCAGACAGAGCAAAATTTCCATCAATACCGCCGAGTCGGATTTGGGCTACACGCGCATTACCGCGACGATGGACGGCACGGTGGTGGCGATTCCCGTGGAAGAGGGGCAGACTGTGAACGCGGCGCAGTCTACGCCGACGATTGTCCAATTGGCGAATCTGGATATGATGTTGAACAAAATGCAGATTGCCGAGGGCGATATTACCAAGGTGAAGGCGGGGCAGGATATTTCGTTTACGATTTTGTCCGAACCGGATACGCCGATTAAGGCGAAGCTCGACAGCGTCGACCCCGGGCTGACCACGATGTCGTCGGGCGGCTACAACAGCAGTACGGATACGGCTTCCAATGCGGTCTATTATTATGCCCGTTCGTTTGTGCCGAATCCGGACGGCAAACTCGCCACGGGGATGACGACGCAGAATACGGTTGAAATCGACGGTGTGAAAAATGTGTTGCTTATTCCGTCGCTGACCGTGAAAAATCGCGGCGGCAAGGCGTTCGTACGCGTGTTGGGTGCGGACGGCAAGGCAGTGGAACGCGAAATCCGGACCGGTATGAAAGACAGTATGAATACCGAAGTGAAAAGCGGGTTGAAAGAGGGGGACAAAGTGGTCATCTCCGAAATAACCGCCGCCGAGCAGCAGGAAAGCGGCGAACGCGCCCTAGGCGGCCCGCCGCGCCGATAA

cDNA: SEQ ID NO: 77

TTATCGGCGCGGCGGGCCGCCTAGGGCGCGTTCGCCGCTTTCCTGCTGCTCGGCGGCGGTTATTTCGGAGATGACCACTTTGTCCCCCTCTTTCAACCCGCTTTTCACTTCGGTATTCATACTGTCTTTCATACCGGTCCGGATTTCGCGTTCCACTGCCTTGCCGTCCGCACCCAACACGCGTACGAACGCCTTGCCGCCGCGATTTTTCACGGTCAGCGACGGAATAAGCAACACATTTTTCACACCGTCGATTTCAACCGTATTCTGCGTCGTCATCCCCGTGGCGAGTTTGCCGTCCGGATTCGGCACAAACGAACGGGCATAATAATAGACCGCATTGGAAGCCGTATCCGTACTGCTGTTGTAGCCGCCCGACGACATCGTGGTCAGCCCGGGGTCGACGCTGTCGAGCTTCGCCTTAATCGGCGTATCCGGTTCGGACAAAATCGTAAACGAAATATCCTGCCCCGCCTTCACCTTGGTAATATCGCCCTCGGCAATCTGCATTTTGTTCAACATCATATCCAGATTCGCCAATTGGACAATCGTCGGCGTAGACTGCGCCGCGTTCACAGTCTGCCCCTCTTCCACGGGAATCGCCACCACCGTGCCGTCCATCGTCGCGGTAATGCGCGTGTAGCCCAAATCCGACTCGGCGGTATTGATGGAAATTTTGCTCTGTCTGATTAAAGCCTTCAACTCGGCAACATTGGCTTTGGCGGCGGCAAGCGCATCCTGCGCGCTTTCCAAATCTTCTTTAGAGGTCGCATCATCCTTCCACAACGCCGCCTGACGCTTATATTTCTTCTCCGCGCTGCCCAATGCAATCTGTGCCGACACCAGCTTCGCCTGATACGTTTCCAATTTGGATTTTTCCATATCGATCGTGTTGGTCTGCGTGGTCGAATTGATTTCCGCAATCAAATCGCCCTTTTTGACCTGTTGCCCGAGTTTGACATAAAGCTTTTTAATCTGCCCCGAAGCCTGCGCGCCGACCGATACCAGGTTGGACGGCGAAATCTCGCCCGTCGCGGAAACCGTCCGGCTGATATCGCCGCGCCTGACCGTTTCCGTAATATAAGCAGCCTGCGGTTCGGGCTTCAGATAAGACCATCCGCCCCAAACCGCTGCCGCCGCGACCGCCGCAACAGCCGCCCATTTCATCATTTTTGCCAT

RNA: SEQ ID NO: 78

AUGGCAAAAAUGAUGAAAUGGGCGGCUGUUGCGGCGGUCGCGGCGGCAGCGGUUUGGGGCGGAUGGUCUUAUCUGAAGCCCGAACCGCAGGCUGCUUAUAUUACGGAAACGGUCAGGCGCGGCGAUAUCAGCCGGACGGUUUCCGCGACGGGCGAGAUUUCGCCGUCCAACCUGGUAUCGGUCGGCGCGCAGGCUUCGGGGCAGAUUAAAAAGCUUUAUGUCAAACUCGGGCAACAGGUCAAAAAGGGCGAUUUGAUUGCGGAAAUCAAUUCGACCACGCAGACCAACACGAUCGAUAUGGAAAAAUCCAAAUUGGAAACGUAUCAGGCGAAGCUGGUGUCGGCACAGAUUGCAUUGGGCAGCGCGGAGAAGAAAUAUAAGCGUCAGGCGGCGUUGUGGAAGGAUGAUGCGACCUCUAAAGAAGAUUUGGAAAGCGCGCAGGAUGCGCUUGCCGCCGCCAAAGCCAAUGUUGCCGAGUUGAAGGCUUUAAUCAGACAGAGCAAAAUUUCCAUCAAUACCGCCGAGUCGGAUUUGGGCUACACGCGCAUUACCGCGACGAUGGACGGCACGGUGGUGGCGAUUCCCGUGGAAGAGGGGCAGACUGUGAACGCGGCGCAGUCUACGCCGACGAUUGUCCAAUUGGCGAAUCUGGAUAUGAUGUUGAACAAAAUGCAGAUUGCCGAGGGCGAUAUUACCAAGGUGAAGGCGGGGCAGGAUAUUUCGUUUACGAUUUUGUCCGAACCGGAUACGCCGAUUAAGGCGAAGCUCGACAGCGUCGACCCCGGGCUGACCACGAUGUCGUCGGGCGGCUACAACAGCAGUACGGAUACGGCUUCCAAUGCGGUCUAUUAUUAUGCCCGUUCGUUUGUGCCGAAUCCGGACGGCAAACUCGCCACGGGGAUGACGACGCAGAAUACGGUUGAAAUCGACGGUGUGAAAAAUGUGUUGCUUAUUCCGUCGCUGACCGUGAAAAAUCGCGGCGGCAAGGCGUUCGUACGCGUGUUGGGUGCGGACGGCAAGGCAGUGGAACGCGAAAUCCGGACCGGUAUGAAAGACAGUAUGAAUACCGAAGUGAAAAGCGGGUUGAAAGAGGGGGACAAAGUGGUCAUCUCCGAAAUAACCGCCGCCGAGCAGCAGGAAAGCGGCGAACGCGCCCUAGGCGGCCCGCCGCGCCGAUAA

18. NGO1658: NC_002946.2:c1613531-1613241

DNA (- strand): SEQ ID NO: 79

GTGGAATATTTTATGTTGCTGGCAACAGACGGGGAGGATGTGCATGAAGCGCGTATGGCGGCACGTCCCGAACACTTTAAACGGCTGGAAACGCTGAAATCGGAAGGCCGTCTGCTGACGGCAGGCCCAAACCTGCTGCCGGACAATCCCGAACGTGTTTCGGGCAGCTTGATTGTGGCACAGTTCGAGTCTTTGGATGCGGCGCAGGCTTGGGCTGAAGACGATCCCTATGTTCATGCCGGCGTGTACAGCGAAGTGCTGATCAAGCCGTTTAAAGCGGTG TTCAAATAA

cDNA: SEQ ID NO: 80

TTATTTGAACACCGCTTTAAACGGCTTGATCAGCACTTCGCTGTACACGCCGGCATGAACATAGGGATCGTCTTCAGCCCAAGCCTGCGCCGCATCCAAAGACTCGAACTGTGCCACAATCAAGCTGCCCGAAACACGTTCGGGATTGTCCGGCAGCAGGTTTGGGCCTGCCGTCAGCAGACGGCCTTCCGATTTCAGCGTTTCCAGCCGTTTAAAGTGTTCGGGACGTGCCGCCATACGCGCTTCATGCACATCCTCCCCGTCTGTTGCCAGCAACATAAAA TATTCCAC

RNA: SEQ ID NO: 81

GUGGAAUAUUUUAUGUUGCUGGCAACAGACGGGGAGGAUGUGCAUGAAGCGCGUAUGGCGGCACGUCCCGAACACUUUAAACGGCUGGAAACGCUGAAAUCGGAAGGCCGUCUGCUGACGGCAGGCCCAAACCUGCUGCCGGACAAUCCCGAACGUGUUUCGGGCAGCUUGAUUGUGGCACAGUUCGAGUCUUUGGAUGCGGCGCAGGCUUGGGCUGAAGACGAUCCCUAUGUUCAUGCCGGCGUGUACAGCGAAGUGCUGAUCAAGCCGUUUAAAGCGGUGUUCAAAUAA

19. NGO1659: NC_002946.2:c1614064-1613534

DNA (- strand): SEQ ID NO: 82

ATGAAATTTGTCAGCGACCTTTTGTCCGTCATCTTGTTTTTTGCTACTTATACCGTTACCAAAAATATGATTGCCGCTGCGGCGGTTGCCTTGGTTGCAGGCGTGGTTCAGGCGGCTTTCCTGTATTGGAAGCATAAAAGGCTGGATACGATGCAGTGGGTCGGACTGGTGCTGATTGTCGTATTCGGCGGCGCAACCATTGTTTTGGGCGACAGCCGCTTCATTATGTGGAAGCCGACAGTATTGTTCTGGTGCGGGGCGTTATTCCTGCTGGGCAGCCACCTTGCGGGTAAAAACGGCTTGAAAGCGAGTATCGGCAGGGAGATTCAGCTTCCGGATGCCGTATGGGGAAAATTGACATATATGTGGGTCGGTTTTCTGATTTTTATGGGTATTGCCAACTGGTTTGTGTTTACTAGGTTTGAAGCGCAATGGGTTAACTATAAGATGTTCGGTTCGACTGCGCTGATGCTTTTTTTCTTTATTATTCAGGGTATTTATCTGAGTACCTATCTGAAAAAGGAGGATTGA

cDNA: SEQ ID NO: 83

TCAATCCTCCTTTTTCAGATAGGTACTCAGATAAATACCCTGAATAATAAAGAAAAAAAGCATCAGCGCAGTCGAACCGAACATCTTATAGTTAACCCATTGCGCTTCAAACCTAGTAAACACAAACCAGTTGGCAATACCCATAAAAATCAGAAAACCGACCCACATATATGTCAATTTTCCCCATACGGCATCCGGAAGCTGAATCTCCCTGCCGATACTCGCTTTCAAGCCGTTTTTACCCGCAAGGTGGCTGCCCAGCAGGAATAACGCCCCGCACCAGAACAATACTGTCGGCTTCCACATAATGAAGCGGCTGTCGCCCAAAACAATGGTTGCGCCGCCGAATACGACAATCAGCACCAGTCCGACCCACTGCATCGTATCCAGCCTTTTATGCTTCCAATACAGGAAAGCCGCCTGAACCACGCCTGCAACCAAGGCAACCGCCGCAGCGGCAATCATATTTTTGGTAACGGTATAAGTAGCAAAAAACAAGATGACGGACAAAAGGTCGCTGACAAATTTCAT

RNA: SEQ ID NO: 84

AUGAAAUUUGUCAGCGACCUUUUGUCCGUCAUCUUGUUUUUUGCUACUUAUACCGUUACCAAAAAUAUGAUUGCCGCUGCGGCGGUUGCCUUGGUUGCAGGCGUGGUUCAGGCGGCUUUCCUGUAUUGGAAGCAUAAAAGGCUGGAUACGAUGCAGUGGGUCGGACUGGUGCUGAUUGUCGUAUUCGGCGGCGCAACCAUUGUUUUGGGCGACAGCCGCUUCAUUAUGUGGAAGCCGACAGUAUUGUUCUGGUGCGGGGCGUUAUUCCUGCUGGGCAGCCACCUUGCGGGUAAAAACGGCUUGAAAGCGAGUAUCGGCAGGGAGAUUCAGCUUCCGGAUGCCGUAUGGGGAAAAUUGACAUAUAUGUGGGUCGGUUUUCUGAUUUUUAUGGGUAUUGCCAACUGGUUUGUGUUUACUAGGUUUGAAGCGCAAUGGGUUAACUAUAAGAUGUUCGGUUCGACUGCGCUGAUGCUUUUUUUCUUUAUUAUUCAGGGUAUUUAUCUGAGUACCUAUCUGAAAAAGGAGGAUUGA

20. NGO1673: NC_002946.2:c1629235-1627559

DNA (- strand): SEQ ID NO: 85

ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCGGGCCGAGCATTACTACAATGAGTCGCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGAGGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAGATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACCCAGATGCCGCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGCTCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAAGAGGAAGAGGAAAGCCACACCCTGTATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGTAGCGGGGCATCCGACATCCATTTCGAGTTTTACGAACACAATGCGCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGGTTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGGATACCGCAGGACGGTAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGACGCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGCCGTTCCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCTTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGTAAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGAGATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCCTGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAAACAGGGCATATGGTGTTTTCCACACTGCACACGAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGCGCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGGAAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGTTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTACCGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGAAGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGGTATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATTTGCGCCGGGCCGGTATTTTGAAAATTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

cDNA: SEQ ID NO: 86

CTAATCGTTGGTATTTGCCGTTACCTCTTCCAATGAAGTAATGCCCTGCATAATTTTCAAAATACCGGCCCGGCGCAAATCCACCATACCCTCCTTATAGGCAACGTCCAAAATACCCACTTCCGTACCGTTGTTCATAATCACACGCTGCATTTCTTCGCTGATGGGCATAACCTCATACACGCCCGCACGCCCCTTATAACCCTGCCCCCGGCAACGGTCGCAACCGACGGCGCGGTAAAGTTTCCAATCTTTTGCAAGATCCTCATCGGTGAAACCGACTTCCTTCAAAGCAGAGGCAGACGGGCGTTCCACTTCCTGTTTGCAGCTCGAACACAGCCTGCGTAAAAGACGCTGCGCCATAATCAGGCTGACCGAACTGGCAATATTAAACGGCGCGACACCCATATTCAGCATACGCGACAACGTCGCCGGCGCATTATTCGTGTGCAGTGTGGAAAACACCATATGCCCTGTTTGTGCCGCCTTAATCGCAATATCGGCAGTTTCCAAATCACGAATCTCACCGACCATAATGATGTCCGGGTCCTGACGCAGGAAAGACTTCAAAGCAGCGGCAAAAGTCAGACCCTGCTTATCATTGACGTTAACCTGATTGATGCCCGGCAGGTTAATCTCGGCAGGGTCTTCCGCCGTTGCAATATTTACCGACTCCGTATTCAAAATATTCAAACAGGTATAGAGCGACACCGTCTTACCCGAACCCGTCGGACCGGTTACCAGCACCATCCCGTAAGGACGGTGAATCGCTTCCAACAACAATTTTTTCTGGAACGGCTCAAAACCGAGCTGGTCGATGTTCAAAGACGCGGCATCGGAATTCAAAATCCGCATCACGACCTTTTCGCCAAACAGCGTCGGCAATGTGCTGACACGGAAATCGACAGGCTTGCCGCCCTTTTGAAAGGTCAGCTGCATCCTACCGTCCTGCGGTATCCGTTTTTCGGAAATGTCCAAACGCGACATTACCTTAATCCGGGAAGCAAGCTGCCCCCTTACCGCAATGGGCGGCTGAACCACCTCGCGGAGCTGCCCGTCCACACGGAAACGGATACGCGCATTGTGTTCGTAAAACTCGAAATGGATGTCGGATGCCCCGCTACGCAAGGCATCCGACAAAGTTTTATGGATAAACCTCGGAACAGGGCCGTCTTCTGCCTCCTCGTTGTCGATATACAGGGTGTGGCTTTCCTCTTCCTCTTGCCCCTCCCCAAGCTCCTGAAGCAGCGATGTCGAACGCGAACCCACCCAATCGAGCAAACCCGCCAACTGGTCATCCTCGACAATGACCAACTCAACCGCAATCCCTGCGGCAGAAACGGTTTTCTGAATTTGCGGCATCTGGGTCGGATCGGAAACCGCAAAAAATACTTTGTCGCCCCGACGGAAAACCGGCACACAGTGGAACTCCACCATCTGCTCCTCCGTCAACACCCCCATCAGCACCCTGTGGCGCGGATAATGACGCAAATCAAGAATCGAATAACTGAACACCCTCGCAATCAATGCCGCAAGCGACTTGGGCGAAATGACACCGTCTGAAAACAGCATCGGCAACACTTCCTTACCCGCCTGCGACTCATTGTAGTAATGCTCGGCCCGCTCAACAGTAACCACCTGGTTTTGAACCAGAATCCTCAGCAAACCTACGCTCAT

RNA: SEQ ID NO: 87

AUGAGCGUAGGUUUGCUGAGGAUUCUGGUUCAAAACCAGGUGGUUACUGUUGAGCGGGCCGAGCAUUACUACAAUGAGUCGCAGGCGGGUAAGGAAGUGUUGCCGAUGCUGUUUUCAGACGGUGUCAUUUCGCCCAAGUCGCUUGCGGCAUUGAUUGCGAGGGUGUUCAGUUAUUCGAUUCUUGAUUUGCGUCAUUAUCCGCGCCACAGGGUGCUGAUGGGGGUGUUGACGGAGGAGCAGAUGGUGGAGUUCCACUGUGUGCCGGUUUUCCGUCGGGGCGACAAAGUAUUUUUUGCGGUUUCCGAUCCGACCCAGAUGCCGCAAAUUCAGAAAACCGUUUCUGCCGCAGGGAUUGCGGUUGAGUUGGUCAUUGUCGAGGAUGACCAGUUGGCGGGUUUGCUCGAUUGGGUGGGUUCGCGUUCGACAUCGCUGCUUCAGGAGCUUGGGGAGGGGCAAGAGGAAGAGGAAAGCCACACCCUGUAUAUCGACAACGAGGAGGCAGAAGACGGCCCUGUUCCGAGGUUUAUCCAUAAAACUUUGUCGGAUGCCUUGCGUAGCGGGGCAUCCGACAUCCAUUUCGAGUUUUACGAACACAAUGCGCGUAUCCGUUUCCGUGUGGACGGGCAGCUCCGCGAGGUGGUUCAGCCGCCCAUUGCGGUAAGGGGGCAGCUUGCUUCCCGGAUUAAGGUAAUGUCGCGUUUGGACAUUUCCGAAAAACGGAUACCGCAGGACGGUAGGAUGCAGCUGACCUUUCAAAAGGGCGGCAAGCCUGUCGAUUUCCGUGUCAGCACAUUGCCGACGCUGUUUGGCGAAAAGGUCGUGAUGCGGAUUUUGAAUUCCGAUGCCGCGUCUUUGAACAUCGACCAGCUCGGUUUUGAGCCGUUCCAGAAAAAAUUGUUGUUGGAAGCGAUUCACCGUCCUUACGGGAUGGUGCUGGUAACCGGUCCGACGGGUUCGGGUAAGACGGUGUCGCUCUAUACCUGUUUGAAUAUUUUGAAUACGGAGUCGGUAAAUAUUGCAACGGCGGAAGACCCUGCCGAGAUUAACCUGCCGGGCAUCAAUCAGGUUAACGUCAAUGAUAAGCAGGGUCUGACUUUUGCCGCUGCUUUGAAGUCUUUCCUGCGUCAGGACCCGGACAUCAUUAUGGUCGGUGAGAUUCGUGAUUUGGAAACUGCCGAUAUUGCGAUUAAGGCGGCACAAACAGGGCAUAUGGUGUUUUCCACACUGCACACGAAUAAUGCGCCGGCGACGUUGUCGCGUAUGCUGAAUAUGGGUGUCGCGCCGUUUAAUAUUGCCAGUUCGGUCAGCCUGAUUAUGGCGCAGCGUCUUUUACGCAGGCUGUGUUCGAGCUGCAAACAGGAAGUGGAACGCCCGUCUGCCUCUGCUUUGAAGGAAGUCGGUUUCACCGAUGAGGAUCUUGCAAAAGAUUGGAAACUUUACCGCGCCGUCGGUUGCGACCGUUGCCGGGGGCAGGGUUAUAAGGGGCGUGCGGGCGUGUAUGAGGUUAUGCCCAUCAGCGAAGAAAUGCAGCGUGUGAUUAUGAACAACGGUACGGAAGUGGGUAUUUUGGACGUUGCCUAUAAGGAGGGUAUGGUGGAUUUGCGCCGGGCCGGUAUUUUGAAAAUUAUGCAGGGCAUUACUUCAUUGGAAGAGGUAACGGCAAAUACCAACGAUUAG

21. Ngo1676: Nc_002946.2:1631221-1631529

DNA (+ strand): SEQ ID NO: 88

ATGTACGCGGTCGTAAAAACCGGCGGCAAACAGTATAAAGTTTCCGTCGGCGAAAAATTGAAAGTAGAACAGATACCAGCCCAACTCGACAGCCAAATCGAACTGACCGAAGTTTTGATGATTGCTGACGGCGAATCTGTAAAAGTTGGCGCACCCTTTATCGAAGGTGCAAAAGTAACGGCTAAAGTAGTGGCACACGGTCGTGGCGAAAAAGTCCGCATCTTCAAAATGCGCCGCCGCAAACACTACCAAAAACGCCAAGGCCACCGCCAAAATTTCACCCAAATCGAAATCGTGGCAATCGCCTAA

cDNA: SEQ ID NO: 89

TTAGGCGATTGCCACGATTTCGATTTGGGTGAAATTTTGGCGGTGGCCTTGGCGTTTTTGGTAGTGTTTGCGGCGGCGCATTTTGAAGATGCGGACTTTTTCGCCACGACCGTGTGCCACTACTTTAGCCGTTACTTTTGCACCTTCGATAAAGGGTGCGCCAACTTTTACAGATTCGCCGTCAGCAATCATCAAAACTTCGGTCAGTTCGATTTGGCTGTCGAGTTGGGCTGGTATCTGTTCTACTTTCAATTTTTCGCCGACGGAAACTTTATACTGTTTGCCGCCGGTTTTTACGACCGCGTACAT

RNA: SEQ ID NO: 90

AUGUACGCGGUCGUAAAAACCGGCGGCAAACAGUAUAAAGUUUCCGUCGGCGAAAAAUUGAAAGUAGAACAGAUACCAGCCCAACUCGACAGCCAAAUCGAACUGACCGAAGUUUUGAUGAUUGCUGACGGCGAAUCUGUAAAAGUUGGCGCACCCUUUAUCGAAGGUGCAAAAGUAACGGCUAAAGUAGUGGCACACGGUCGUGGCGAAAAAGUCCGCAUCUUCAAAAUGCGCCGCCGCAAACACUACCAAAAACGCCAAGGCCACCGCCAAAAUUUCACCCAAAUCGAAAUCGUGGCAAUCGCCUAA

22. Ngo1677: Nc_002946.2:1631554-1631826

DNA (+ strand): SEQ ID NO: 91

ATGGCAAGTAAAAAAGCAGGCGGCAGCACCCGCAACGGTCGCGATTCAGAAGCCAAACGCTTGGGCGTTAAAGCCTACGGCAACGAGCTGATTCCCGCAGGTTCCATCATCGTACGCCAACGCGGTACCAAATTCCACGCAGGCGACAACGTAGGTATGGGCAAAGACCACACTTTGTTCGCCAAAATTGACGGTTATGTCGAATTCAAAACCAAAGGCGCGCTGAACCGTAAAACTGTCAGCATCCGTCCTTACACCGGTTCTGAAGAATAA

cDNA: SEQ ID NO: 92

TTATTCTTCAGAACCGGTGTAAGGACGGATGCTGACAGTTTTACGGTTCAGCGCGCCTTTGGTTTTGAATTCGACATAACCGTCAATTTTGGCGAACAAAGTGTGGTCTTTGCCCATACCTACGTTGTCGCCTGCGTGGAATTTGGTACCGCGTTGGCGTACGATGATGGAACCTGCGGGAATCAGCTCGTTGCCGTAGGCTTTAACGCCCAAGCGTTTGGCTTCTGAATCGCGACCGTTGCGGGTGCTGCCGCCTGCTTTTTTACTTGCCAT

RNA: SEQ ID NO: 93

AUGGCAAGUAAAAAAGCAGGCGGCAGCACCCGCAACGGUCGCGAUUCAGAAGCCAAACGCUUGGGCGUUAAAGCCUACGGCAACGAGCUGAUUCCCGCAGGUUCCAUCAUCGUACGCCAACGCGGUACCAAAUUCCACGCAGGCGACAACGUAGGUAUGGGCAAAGACCACACUUUGUUCGCCAAAAUUGACGGUUAUGUCGAAUUCAAAACCAAAGGCGCGCUGAACCGUAAAACUGUCAGCAUCCGUCCUUACACCGGUUCUGAAGAAUAA

23. NGO1679: NC_002946.2:c1633589-1633434

DNA (- strand): SEQ ID NO: 94

ATGCGCGATAAAATCAAACTGGAATCCGGTGCAGGTACTGGCCACTTCTACACCACTACCAAAAATAAACGCACTATGCCCGGCAAACTGGAAATCAAAAAATTCGATCCGGTTGCCCGCAAACACGTAGTGTACAAAGAAACCAAACTGAAATAA

cDNA: SEQ ID NO: 95

TTATTTCAGTTTGGTTTCTTTGTACACTACGTGTTTGCGGGCAACCGGATCGAATTTTTTGATTTCCAGTTTGCCGGGCATAGTGCGTTTATTTTTGGTAGTGGTGTAGAAGTGGCCAGTACCTGCACCGGATTCCAGTTTGATTTTATCGCGCAT

RNA: SEQ ID NO: 96

AUGCGCGAUAAAAUCAAACUGGAAUCCGGUGCAGGUACUGGCCACUUCUACACCACUACCAAAAAUAAACGCACUAUGCCCGGCAAACUGGAAAUCAAAAAAUUCGAUCCGGUUGCCCGCAAACACGUAGUGUACAAAGAAACCAAACUGAAAUAA

24. Ngo1804: Nc_002946.2:1777677-1778126

DNA (+ strand): SEQ ID NO: 97

ATGGACGTACAACTCCCCATCGAAGCCAAAGACATCCAAAAACTCATCCCCCACCGCTACCCGTTTCTCCAGCTCGACCGCATTACCGCCTTCGAGCCGATGAAAACCCTGACCGCCATCAAAAACGTAACCATAAACGAACCCCAATTCCAAGGCCATTTCCCCGACCTGCCCGTTATGCCCGGCGTACTCATCATCGAAGCGATGGCGCAGGCGTGCGGCACGTTGGCGATTTTGAGCGAAGGCGGGCGCAAGGAAAACGAATTTTTCTTCTTCGCCGGCATAGACGAAGCCCGTTTCAAACGCCAAGTCATCCCCGGCGACCAACTCGTCTTTGAAGTCGAACTCCTGACCAGCCGGCGCGGCATCGGCAAATTCAACGCCGTTGCCAAAGTGGACGGACAAGTCGCCGTCGAAGCCGTGATTATGTGCGCCAAACGCGTGGTTTGA

cDNA: SEQ ID NO: 98

TCAAACCACGCGTTTGGCGCACATAATCACGGCTTCGACGGCGACTTGTCCGTCCACTTTGGCAACGGCGTTGAATTTGCCGATGCCGCGCCGGCTGGTCAGGAGTTCGACTTCAAAGACGAGTTGGTCGCCGGGGATGACTTGGCGTTTGAAACGGGCTTCGTCTATGCCGGCGAAGAAGAAAAATTCGTTTTCCTTGCGCCCGCCTTCGCTCAAAATCGCCAACGTGCCGCACGCCTGCGCCATCGCTTCGATGATGAGTACGCCGGGCATAACGGGCAGGTCGGGGAAATGGCCTTGGAATTGGGGTTCGTTTATGGTTACGTTTTTGATGGCGGTCAGGGTTTTCATCGGCTCGAAGGCGGTAATGCGGTCGAGCTGGAGAAACGGGTAGCGGTGGGGGATGAGTTTTTGGATGTCTTTGGCTTCGATGGGGAGTTGTACGTCCAT

RNA: SEQ ID NO: 99

AUGGACGUACAACUCCCCAUCGAAGCCAAAGACAUCCAAAAACUCAUCCCCCACCGCUACCCGUUUCUCCAGCUCGACCGCAUUACCGCCUUCGAGCCGAUGAAAACCCUGACCGCCAUCAAAAACGUAACCAUAAACGAACCCCAAUUCCAAGGCCAUUUCCCCGACCUGCCCGUUAUGCCCGGCGUACUCAUCAUCGAAGCGAUGGCGCAGGCGUGCGGCACGUUGGCGAUUUUGAGCGAAGGCGGGCGCAAGGAAAACGAAUUUUUCUUCUUCGCCGGCAUAGACGAAGCCCGUUUCAAACGCCAAGUCAUCCCCGGCGACCAACUCGUCUUUGAAGUCGAACUCCUGACCAGCCGGCGCGGCAUCGGCAAAUUCAACGCCGUUGCCAAAGUGGACGGACAAGUCGCCGUCGAAGCCGUGAUUAUGUGCGCCAAAC GCGUGGUUUGA

25. NGO1833: NC_002946.2:c1803866-1803537

DNA (- strand): SEQ ID NO: 100

ATGAGAGTAAATGCACAACATAAAAATGCCCGTATCTCTGCTCAAAAGGCTCGTTTGGTAGCTGATTTGATTCGTGGTAAAGACGTTGCCCAAGCTTTGAATATTTTGGCTTTCAGCCCTAAAAAAGGTGCCGAGCTGATCAAAAAAGTATTGGAGTCAGCTATTGCTAATGCTGAGCATAATAACGGTGCGGACATTGATGAACTGAAAGTGGTAACTATCTTTGTTGACAAAGGTCCAAGCTTGAAACGTTTTCAAGCTCGCGCCAAAGGTCGCGGTAACCGCATCGAAAAACAAACTTGTCATATCAATGTGACAGTGGGTAACTAA

cDNA: SEQ ID NO: 101

TTAGTTACCCACTGTCACATTGATATGACAAGTTTGTTTTTCGATGCGGTTACCGCGACCTTTGGCGCGAGCTTGAAAACGTTTCAAGCTTGGACCTTTGTCAACAAAGATAGTTACCACTTTCAGTTCATCAATGTCCGCACCGTTATTATGCTCAGCATTAGCAATAGCTGACTCCAATACTTTTTTGATCAGCTCGGCACCTTTTTTAGGGCTGAAAGCCAAAATATTCAAAGCTTGGGCAACGTCTTTACCACGAATCAAATCAGCTACCAAACGAGCCTTTTGAGCAGAGATACGGGCATTTTTATGTTGTGCATTTACTCTCAT

RNA: SEQ ID NO: 102

AUGAGAGUAAAUGCACAACAUAAAAAUGCCCGUAUCUCUGCUCAAAAGGCUCGUUUGGUAGCUGAUUUGAUUCGUGGUAAAGACGUUGCCCAAGCUUUGAAUAUUUUGGCUUUCAGCCCUAAAAAAGGUGCCGAGCUGAUCAAAAAAGUAUUGGAGUCAGCUAUUGCUAAUGCUGAGCAUAAUAACGGUGCGGACAUUGAUGAACUGAAAGUGGUAACUAUCUUUGUUGACAAAGGUCCAAGCUUGAAACGUUUUCAAGCUCGCGCCAAAGGUCGCGGUAACCGCAUCGAAAAACAAACUUGUCAUAUCAAUGUGACAGUGGGU AACUAA

26. NGO1834: NC_002946.2:c1804153-1803875

DNA (- strand): SEQ ID NO: 103

ATGGCTCGTTCATTGAAAAAAGGCCCATATGTAGACCTGCATTTGCTGAAAAAAGTAGATGCTGTTCGCGCAAGCAACGACAAACGCCCGATTAAAACCTGGTCTCGTCGTTCTACCATTCTGCCTGATTTTATCGGTCTGACCATTGCCGTGCACAACGGTCGCACCCATGTGCCTGTGTTTATCAGCGACAATATGGTTGGTCATAAATTAGGCGAATTCTCATTGACCCGTACCTTTAAAGGCCACCTGGCCGATAAAAAGGCTAAAAAGAAATAA

cDNA: SEQ ID NO: 104

TTATTTCTTTTTAGCCTTTTTATCGGCCAGGTGGCCTTTAAAGGTACGGGTCAATGAGAATTCGCCTAATTTATGACCAACCATATTGTCGCTGATAAACACAGGCACATGGGTGCGACCGTTGTGCACGGCAATGGTCAGACCGATAAAATCAGGCAGAATGGTAGAACGACGAGACCAGGTTTTAATCGGGCGTTTGTCGTTGCTTGCGCGAACAGCATCTACTTTTTTCAGCAAATGCAGGTCTACATATGGGCCTTTTTTCAATGAACGAGCCAT

RNA: SEQ ID NO: 105

AUGGCUCGUUCAUUGAAAAAAGGCCCAUAUGUAGACCUGCAUUUGCUGAAAAAAGUAGAUGCUGUUCGCGCAAGCAACGACAAACGCCCGAUUAAAACCUGGUCUCGUCGUUCUACCAUUCUGCCUGAUUUUAUCGGUCUGACCAUUGCCGUGCACAACGGUCGCACCCAUGUGCCUGUGUUUAUCAGCGACAAUAUGGUUGGUCAUAAAUUAGGCGAAUUCUCAUUGACCCGUACCUUUAAAGGCCACCUGGCCGAUAAAAAGGCUAAAAAG AAAUAA

27. NGO1835: NC_002946.2:c1804992-1804159

DNA (- strand): SEQ ID NO: 106

ATGGCAATCGTTAAAATGAAGCCGACCTCTGCAGGCCGTCGCGGCATGGTTCGCGTGGTAACAGAAGGTTTGCACAAAGGTGCACCTTATGCACCCTTGCTTGAAAAGAAAAATTCTACTGCCGGCCGTAACAATAATGGTCATATCACCACCCGTCACAAAGGCGGCGGTCATAAACACCATTACCGTGTTGTAGACTTTAAACGTAACAAAGACGGCATTTCTGCTAAAGTAGAGCGTATCGAATACGATCCTAACCGTACTGCCTTCATCGCACTGTTGTGCTATGCAGACGGCGAGCGTCGTTACATCATCGCTCCTCGCGGTATTCAAGCCGGTGTCGTATTGGTTTCCGGTGCTGAAGCTGCCATCAAAGTAGGCAACACCCTGCCGATCCGCAACATTCCCGTTGGTACGACTATCCACTGTATCGAAATGAAACCCGGTAAAGGTGCTCAAATCGCACGTTCTGCCGGTGCTTCTGCGGTATTGTTGGCTAAAGAAGGTGCATACGCTCAAGTCCGTCTGCGCTCTGGCGAAGTTCGTAAAATCAACGTAGATTGCCGTGCGACCATCGGTGAAGTCGGTAACGAAGAGCAAAGCCTGAAAAAAATCGGTAAAGCCGGTGCTAACCGTTGGCGCGGTATTCGTCCGACCGTACGCGGTGTTGTCATGAATCCCGTCGATCACCCGCATGGTGGTGGTGAAGGCCGTACCGGCGAAGCCCGCGAACCGGTTAGTCCATGGGGTACTCCTGCTAAAGGCTACCGCACTCGTAATAACAAACGCACGGATAATATGATTGTTCGTCGCCGTTACTCAAATAAAGGTTAA

cDNA: SEQ ID NO: 107

TTAACCTTTATTTGAGTAACGGCGACGAACAATCATATTATCCGTGCGTTTGTTATTACGAGTGCGGTAGCCTTTAGCAGGAGTACCCCATGGACTAACCGGTTCGCGGGCTTCGCCGGTACGGCCTTCACCACCACCATGCGGGTGATCGACGGGATTCATGACAACACCGCGTACGGTCGGACGAATACCGCGCCAACGGTTAGCACCGGCTTTACCGATTTTTTTCAGGCTTTGCTCTTCGTTACCGACTTCACCGATGGTCGCACGGCAATCTACGTTGATTTTACGAACTTCGCCAGAGCGCAGACGGACTTGAGCGTATGCACCTTCTTTAGCCAACAATACCGCAGAAGCACCGGCAGAACGTGCGATTTGAGCACCTTTACCGGGTTTCATTTCGATACAGTGGATAGTCGTACCAACGGGAATGTTGCGGATCGGCAGGGTGTTGCCTACTTTGATGGCAGCTTCAGCACCGGAAACCAATACGACACCGGCTTGAATACCGCGAGGAGCGATGATGTAACGACGCTCGCCGTCTGCATAGCACAACAGTGCGATGAAGGCAGTACGGTTAGGATCGTATTCGATACGCTCTACTTTAGCAGAAATGCCGTCTTTGTTACGTTTAAAGTCTACAACACGGTAATGGTGTTTATGACCGCCGCCTTTGTGACGGGTGGTGATATGACCATTATTGTTACGGCCGGCAGTAGAATTTTTCTTTTCAAGCAAGGGTGCATAAGGTGCACCTTTGTGCAAACCTTCTGTTACCACGCGAACCATGCCGCGACGGCCTGCAGAGGTCGGCTTCATTTTAACGATTGCCAT

RNA: SEQ ID NO: 108

AUGGCAAUCGUUAAAAUGAAGCCGACCUCUGCAGGCCGUCGCGGCAUGGUUCGCGUGGUAACAGAAGGUUUGCACAAAGGUGCACCUUAUGCACCCUUGCUUGAAAAGAAAAAUUCUACUGCCGGCCGUAACAAUAAUGGUCAUAUCACCACCCGUCACAAAGGCGGCGGUCAUAAACACCAUUACCGUGUUGUAGACUUUAAACGUAACAAAGACGGCAUUUCUGCUAAAGUAGAGCGUAUCGAAUACGAUCCUAACCGUACUGCCUUCAUCGCACUGUUGUGCUAUGCAGACGGCGAGCGUCGUUACAUCAUCGCUCCUCGCGGUAUUCAAGCCGGUGUCGUAUUGGUUUCCGGUGCUGAAGCUGCCAUCAAAGUAGGCAACACCCUGCCGAUCCGCAACAUUCCCGUUGGUACGACUAUCCACUGUAUCGAAAUGAAACCCGGUAAAGGUGCUCAAAUCGCACGUUCUGCCGGUGCUUCUGCGGUAUUGUUGGCUAAAGAAGGUGCAUACGCUCAAGUCCGUCUGCGCUCUGGCGAAGUUCGUAAAAUCAACGUAGAUUGCCGUGCGACCAUCGGUGAAGUCGGUAACGAAGAGCAAAGCCUGAAAAAAAUCGGUAAAGCCGGUGCUAACCGUUGGCGCGGUAUUCGUCCGACCGUACGCGGUGUUGUCAUGAAUCCCGUCGAUCACCCGCAUGGUGGUGGUGAAGGCCGUACCGGCGAAGCCCGCGAACCGGUUAGUCCAUGGGGUACUCCUGCUAAAGGCUACCGCACUCGUAAUAACAAACGCACGGAUAAUAUGAUUGUUCGUCGCCGUUAC UCAAAUAAAGGUUAA

28. NGO1837: NC_002946.2:c1805929-1805309

DNA (- strand): SEQ ID NO: 109

ATGGAATTGAAAGTAATTGACGCTAAAGGACAAGTTTCAGGCAGCCTGTCTGTTTCTGATGCTTTGTTCGCCCGCGAATACAATGAAGCGTTGGTTCACCAGCTGGTAAATGCCTACTTGGCAAACGCCCGCTCTGGTAACCGTGCTCAAAAAACCCGTGCCGAAGTAAAACACTCAACCAAAAAACCATGGCGTCAAAAAGGTACCGGCCGCGCCCGTTCCGGTATGACTTCTTCTCCGCTGTGGCGTAAAGGCGGTCGCGCGTTCCCGAACAAACCCGACGAAAACTTCACTCAAAAAGTAAACCGTAAAATGTACCGTGCCGGTATGGCGACTATCCTGTCCCAATTGGCGCGTGACGAGCGTTTGTTTGTGATTGAGGCGTTGACTGCCGAAACTCCCAAAACCAAAGTTTTTGCCGAACAAGTAAAAAATTTGGCTCTGGAGCAAGTGCTGTTTGTAACCAAACGGCTCGACGAGAATGTTTACTTGGCTTCACGCAACTTGCCAAACGTATTGGTTTTGGAAGCTCAACAAGTTGATCCTTACAGCTTGCTGCGTTATAAAAAAGTAATCATCACTAAAGATGCGGTTGCACAATTAGAGGAGCAATGGGTATGA

cDNA: SEQ ID NO: 110

TCATACCCATTGCTCCTCTAATTGTGCAACCGCATCTTTAGTGATGATTACTTTTTTATAACGCAGCAAGCTGTAAGGATCAACTTGTTGAGCTTCCAAAACCAATACGTTTGGCAAGTTGCGTGAAGCCAAGTAAACATTCTCGTCGAGCCGTTTGGTTACAAACAGCACTTGCTCCAGAGCCAAATTTTTTACTTGTTCGGCAAAAACTTTGGTTTTGGGAGTTTCGGCAGTCAACGCCTCAATCACAAACAAACGCTCGTCACGCGCCAATTGGGACAGGATAGTCGCCATACCGGCACGGTACATTTTACGGTTTACTTTTTGAGTGAAGTTTTCGTCGGGTTTGTTCGGGAACGCGCGACCGCCTTTACGCCACAGCGGAGAAGAAGTCATACCGGAACGGGCGCGGCCGGTACCTTTTTGACGCCATGGTTTTTTGGTTGAGTGTTTTACTTCGGCACGGGTTTTTTGAGCACGGTTACCAGAGCGGGCGTTTGCCAAGTAGGCATTTACCAGCTGGTGAACCAACGCTTCATTGTATTCGCGGGCGAACAAAGCATCAGAAACAGACAGGCTGCCTGAAACTTGTCCTTTAGCGTCAATTACTTTCAATTCCAT

RNA: SEQ ID NO: 111

AUGGAAUUGAAAGUAAUUGACGCUAAAGGACAAGUUUCAGGCAGCCUGUCUGUUUCUGAUGCUUUGUUCGCCCGCGAAUACAAUGAAGCGUUGGUUCACCAGCUGGUAAAUGCCUACUUGGCAAACGCCCGCUCUGGUAACCGUGCUCAAAAAACCCGUGCCGAAGUAAAACACUCAACCAAAAAACCAUGGCGUCAAAAAGGUACCGGCCGCGCCCGUUCCGGUAUGACUUCUUCUCCGCUGUGGCGUAAAGGCGGUCGCGCGUUCCCGAACAAACCCGACGAAAACUUCACUCAAAAAGUAAACCGUAAAAUGUACCGUGCCGGUAUGGCGACUAUCCUGUCCCAAUUGGCGCGUGACGAGCGUUUGUUUGUGAUUGAGGCGUUGACUGCCGAAACUCCCAAAACCAAAGUUUUUGCCGAACAAGUAAAAAAUUUGGCUCUGGAGCAAGUGCUGUUUGUAACCAAACGGCUCGACGAGAAUGUUUACUUGGCUUCACGCAACUUGCCAAACGUAUUGGUUUUGGAAGCUCAACAAGUUGAUCCUUACAGCUUGCUGCGUUAUAAAAAAGUAAUCAUCACUAAAGAUGCGGUUGCACAAUUAGAGGAGCAAUGGGUAUGA

29. NGO1843: NC_002946.2:c1811065-1808960

DNA (- strand): SEQ ID NO: 112

ATGGCTCGTAAGACCCCGATCAGCCTGTACCGCAACATCGGTATTTCCGCCCATATCGATGCGGGTAAAACCACGACGACAGAACGTATTTTGTTCTATACCGGTTTGACCCACAAGCTGGGCGAAGTGCATGACGGTGCGGCTACTACCGACTACATGGAACAAGAGCAAGAGCGCGGTATTACCATTACCTCCGCTGCCGTTACTTCCTACTGGTCCGGTATGGCGAAACAATTCCCCGAGCACCGCTTCAACATCATCGACACCCCGGGGCACGTTGACTTTACCGTAGAGGTAGAGCGTTCTATGCGTGTATTGGACGGCGCGGTAATGGTTTACTGTGCGGTGGGCGGTGTTCAACCGCAATCTGAAACCGTATGGCGGCAAGCCAACAAATACCAAGTTCCGCGCTTGGCGTTTGTCAATAAAATGGACCGCCAAGGTGCCAACTTCTTCCGCGTTGTCGAGCAAATGAAAACCCGTTTGCGCGCAAACCCCGTACCTATCGTCATTCCGGTAGGCGCGGAAGACAGTTTTACCGGTGTTGTCGATTTGCTGAAAATGAAATCTATCATCTGGAATGAAGCCGATAAAGGTACAACCTTTACCTATGGCGATATTCCTGCCGAATTGGTCGAAACTGCCGAAGAATGGCGTCAAAATATGATTGAAGCCGCAGCCGAAGCCAGCGAAGAACTGATGGACAAATACTTGGGCGGTGAAGATCTGGCCGAAGAAGAAATCGTAGGCGCGTTGCGTCAACGTACTTTGGCAGGCGAAATTCAGCCTATGCTGTGCGGTTCTGCATTTAAAAACAAAGGTGTTCAACGTATGTTGGACGCAGTTGTAGAATTGCTGCCAGCTCCTACCGATATTCCTCCGGTTCAAGGTGTTAATCCTAACACTGAAGAAGCCGACAGCCGTCAAGCCAGCGATGAAGAGAAATTCTCTGCATTGGCATTCAAAATGTTGAACGACAAATACGTCGGTCAGCTGACCTTTATCCGCGTTTACTCAGGCGTAGTAAAATCCGGCGATACCGTACTGAATTCTGTAAAAGGCACTCGCGAACGTATCGGTCGTTTGGTGCAAATGACTGCCGCAGACCGTACTGAAATCGAAGAAGTACGCGCTGGCGACATCGCAGCCGCTATCGGTCTGAAAGACGTTACTACCGGTGAAACCTTGTGTGCGGAAAGCGCGCCGATTATCTTGGAACGTATGGAATTCCCCGAGCCGGTAATCCATATTGCCGTTGAGCCGAAAACCAAAGCCGACCAAGAGAAAATGGGTATCGCCCTGAACCGCTTGGCTAAAGAAGACCCTTCTTTCCGCGTTCGTACAGACGAAGAATCCGGTCAAACCATTATTTCCGGTATGGGTGAGCTGCACTTGGAAATTATTGTTGACCGTATGAAACGCGAATTCGGTGTGGAAGCAAATATCGGTGCACCTCAAGTGGCTTACCGTGAAACTATCCGCAAAGCCGTTAAAGCTGAATACAAACATGCAAAACAATCCGGTGGTAAAGGTCAATACGGTCACGTTGTGATTGAAATGGAACCTATGGAACCGGGTGGTGAAGGTTACGAGTTTATCGATGAAATTAAAGGTGGTGTGATTCCTCGCGAATTTATTCCGTCTGTCGATAAAGGTATCCGCGATACGTTGCCTAACGGTATCGTTGCCGGCTATCCTGTAGTTGACGTACGTATCCGTCTGGTATTCGGTTCTTACCATGATGTCGACTCTTCCCAATTGGCATTTGAATTGGCTGCTTCTCAAGCGTTTAAAGAAGGTATGCGTCAAGCATCTCCTGCCCTGCTTGAGCCGATTATGGCAGTTGAAGTGGAAACTCCGGAAGAATACATGGGCGACGTAATGGGCGACTTGAACCGCCGTCGCGGTGTTGTATTGGGTATGGATGATGACGGTATCGGCGGTAAAAAAGTCCGTGCCGAAGTACCTCTGGCAGAAATGTTCGGTTACTCGACCGACCTGCGTTCTGCAACCCAAGGCCGCGCTACTTACTCTATGGAGTTCAAGAAATATTCTGAAGCTCCTGCCCACATAGCTGCTGCTGTAACTGAAGCCCGTAA AGGCTAA

cDNA: SEQ ID NO: 113

TTAGCCTTTACGGGCTTCAGTTACAGCAGCAGCTATGTGGGCAGGAGCTTCAGAATATTTCTTGAACTCCATAGAGTAAGTAGCGCGGCCTTGGGTTGCAGAACGCAGGTCGGTCGAGTAACCGAACATTTCTGCCAGAGGTACTTCGGCACGGACTTTTTTACCGCCGATACCGTCATCATCCATACCCAATACAACACCGCGACGGCGGTTCAAGTCGCCCATTACGTCGCCCATGTATTCTTCCGGAGTTTCCACTTCAACTGCCATAATCGGCTCAAGCAGGGCAGGAGATGCTTGACGCATACCTTCTTTAAACGCTTGAGAAGCAGCCAATTCAAATGCCAATTGGGAAGAGTCGACATCATGGTAAGAACCGAATACCAGACGGATACGTACGTCAACTACAGGATAGCCGGCAACGATACCGTTAGGCAACGTATCGCGGATACCTTTATCGACAGACGGAATAAATTCGCGAGGAATCACACCACCTTTAATTTCATCGATAAACTCGTAACCTTCACCACCCGGTTCCATAGGTTCCATTTCAATCACAACGTGACCGTATTGACCTTTACCACCGGATTGTTTTGCATGTTTGTATTCAGCTTTAACGGCTTTGCGGATAGTTTCACGGTAAGCCACTTGAGGTGCACCGATATTTGCTTCCACACCGAATTCGCGTTTCATACGGTCAACAATAATTTCCAAGTGCAGCTCACCCATACCGGAAATAATGGTTTGACCGGATTCTTCGTCTGTACGAACGCGGAAAGAAGGGTCTTCTTTAGCCAAGCGGTTCAGGGCGATACCCATTTTCTCTTGGTCGGCTTTGGTTTTCGGCTCAACGGCAATATGGATTACCGGCTCGGGGAATTCCATACGTTCCAAGATAATCGGCGCGCTTTCCGCACACAAGGTTTCACCGGTAGTAACGTCTTTCAGACCGATAGCGGCTGCGATGTCGCCAGCGCGTACTTCTTCGATTTCAGTACGGTCTGCGGCAGTCATTTGCACCAAACGACCGATACGTTCGCGAGTGCCTTTTACAGAATTCAGTACGGTATCGCCGGATTTTACTACGCCTGAGTAAACGCGGATAAAGGTCAGCTGACCGACGTATTTGTCGTTCAACATTTTGAATGCCAATGCAGAGAATTTCTCTTCATCGCTGGCTTGACGGCTGTCGGCTTCTTCAGTGTTAGGATTAACACCTTGAACCGGAGGAATATCGGTAGGAGCTGGCAGCAATTCTACAACTGCGTCCAACATACGTTGAACACCTTTGTTTTTAAATGCAGAACCGCACAGCATAGGCTGAATTTCGCCTGCCAAAGTACGTTGACGCAACGCGCCTACGATTTCTTCTTCGGCCAGATCTTCACCGCCCAAGTATTTGTCCATCAGTTCTTCGCTGGCTTCGGCTGCGGCTTCAATCATATTTTGACGCCATTCTTCGGCAGTTTCGACCAATTCGGCAGGAATATCGCCATAGGTAAAGGTTGTACCTTTATCGGCTTCATTCCAGATGATAGATTTCATTTTCAGCAAATCGACAACACCGGTAAAACTGTCTTCCGCGCCTACCGGAATGACGATAGGTACGGGGTTTGCGCGCAAACGGGTTTTCATTTGCTCGACAACGCGGAAGAAGTTGGCACCTTGGCGGTCCATTTTATTGACAAACGCCAAGCGCGGAACTTGGTATTTGTTGGCTTGCCGCCATACGGTTTCAGATTGCGGTTGAACACCGCCCACCGCACAGTAAACCATTACCGCGCCGTCCAATACACGCATAGAACGCTCTACCTCTACGGTAAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGAAGCGGTGCTCGGGGAATTGTTTCGCCATACCGGACCAGTAGGAAGTAACGGCAGCGGAGGTAATGGTAATACCGCGCTCTTGCTCTTGTTCCATGTAGTCGGTAGTAGCCGCACCGTCATGCACTTCGCCCAGCTTGTGGGTCAAACCGGTATAGAACAAAATACGTTCTGTCGTCGTGGTTTTACCCGCATCGATATGGGCGGAAATACCGATGTTGCGGTACAGGCTGATCGGGGTCTTACGAGCCAT

RNA: SEQ ID NO: 114

AUGGCUCGUAAGACCCCGAUCAGCCUGUACCGCAACAUCGGUAUUUCCGCCCAUAUCGAUGCGGGUAAAACCACGACGACAGAACGUAUUUUGUUCUAUACCGGUUUGACCCACAAGCUGGGCGAAGUGCAUGACGGUGCGGCUACUACCGACUACAUGGAACAAGAGCAAGAGCGCGGUAUUACCAUUACCUCCGCUGCCGUUACUUCCUACUGGUCCGGUAUGGCGAAACAAUUCCCCGAGCACCGCUUCAACAUCAUCGACACCCCGGGGCACGUUGACUUUACCGUAGAGGUAGAGCGUUCUAUGCGUGUAUUGGACGGCGCGGUAAUGGUUUACUGUGCGGUGGGCGGUGUUCAACCGCAAUCUGAAACCGUAUGGCGGCAAGCCAACAAAUACCAAGUUCCGCGCUUGGCGUUUGUCAAUAAAAUGGACCGCCAAGGUGCCAACUUCUUCCGCGUUGUCGAGCAAAUGAAAACCCGUUUGCGCGCAAACCCCGUACCUAUCGUCAUUCCGGUAGGCGCGGAAGACAGUUUUACCGGUGUUGUCGAUUUGCUGAAAAUGAAAUCUAUCAUCUGGAAUGAAGCCGAUAAAGGUACAACCUUUACCUAUGGCGAUAUUCCUGCCGAAUUGGUCGAAACUGCCGAAGAAUGGCGUCAAAAUAUGAUUGAAGCCGCAGCCGAAGCCAGCGAAGAACUGAUGGACAAAUACUUGGGCGGUGAAGAUCUGGCCGAAGAAGAAAUCGUAGGCGCGUUGCGUCAACGUACUUUGGCAGGCGAAAUUCAGCCUAUGCUGUGCGGUUCUGCAUUUAAAAACAAAGGUGUUCAACGUAUGUUGGACGCAGUUGUAGAAUUGCUGCCAGCUCCUACCGAUAUUCCUCCGGUUCAAGGUGUUAAUCCUAACACUGAAGAAGCCGACAGCCGUCAAGCCAGCGAUGAAGAGAAAUUCUCUGCAUUGGCAUUCAAAAUGUUGAACGACAAAUACGUCGGUCAGCUGACCUUUAUCCGCGUUUACUCAGGCGUAGUAAAAUCCGGCGAUACCGUACUGAAUUCUGUAAAAGGCACUCGCGAACGUAUCGGUCGUUUGGUGCAAAUGACUGCCGCAGACCGUACUGAAAUCGAAGAAGUACGCGCUGGCGACAUCGCAGCCGCUAUCGGUCUGAAAGACGUUACUACCGGUGAAACCUUGUGUGCGGAAAGCGCGCCGAUUAUCUUGGAACGUAUGGAAUUCCCCGAGCCGGUAAUCCAUAUUGCCGUUGAGCCGAAAACCAAAGCCGACCAAGAGAAAAUGGGUAUCGCCCUGAACCGCUUGGCUAAAGAAGACCCUUCUUUCCGCGUUCGUACAGACGAAGAAUCCGGUCAAACCAUUAUUUCCGGUAUGGGUGAGCUGCACUUGGAAAUUAUUGUUGACCGUAUGAAACGCGAAUUCGGUGUGGAAGCAAAUAUCGGUGCACCUCAAGUGGCUUACCGUGAAACUAUCCGCAAAGCCGUUAAAGCUGAAUACAAACAUGCAAAACAAUCCGGUGGUAAAGGUCAAUACGGUCACGUUGUGAUUGAAAUGGAACCUAUGGAACCGGGUGGUGAAGGUUACGAGUUUAUCGAUGAAAUUAAAGGUGGUGUGAUUCCUCGCGAAUUUAUUCCGUCUGUCGAUAAAGGUAUCCGCGAUACGUUGCCUAACGGUAUCGUUGCCGGCUAUCCUGUAGUUGACGUACGUAUCCGUCUGGUAUUCGGUUCUUACCAUGAUGUCGACUCUUCCCAAUUGGCAUUUGAAUUGGCUGCUUCUCAAGCGUUUAAAGAAGGUAUGCGUCAAGCAUCUCCUGCCCUGCUUGAGCCGAUUAUGGCAGUUGAAGUGGAAACUCCGGAAGAAUACAUGGGCGACGUAAUGGGCGACUUGAACCGCCGUCGCGGUGUUGUAUUGGGUAUGGAUGAUGACGGUAUCGGCGGUAAAAAAGUCCGUGCCGAAGUACCUCUGGCAGAAAUGUUCGGUUACUCGACCGACCUGCGUUCUGCAACCCAAGGCCGCGCUACUUACUCUAUGGAGUUCAAGAAAUAUUCUGAAGCUCCUGCCCACAUAGCUGCUGCUGUAACUGAAGCCCGUAAAGGCUAA

30. NGO1844: NC_002946.2:c1811554-1811084

DNA (- strand): SEQ ID NO: 115

ATGCCAAGACGTAGAGAAGTCCCCAAGCGCGACGTACTGCCAGATCCTAAATTCGGTAGCGTCGAGTTGACCAAATTCATGAACGTATTGATGATTGACGGTAAAAAATCCGTTGCCGAGCGTATCGTTTACGGTGCGTTGGAACAGATTGAGAAAAAAACCGGCAAAGCAGCAATCGAAGTATTTAACGAAGCCATTGCAAACTCCAAACCTATCGTGGAAGTGAAAAGCCGCCGTGTAGGTGGTGCAAACTACCAAGTTCCTGTTGAAGTTCGTCCTTCACGCCGTCTGGCTTTGGCAATGCGTTGGGTTCGCGACGCGGCCCGCAAACGTGGTGAGAAATCCATGGATCTGCGTTTGGCAGGCGAGTTGATTGATGCGTCCGAAGGCCGTGGCGGTGCGTTGAAAAAACGTGAAGAAGTACACCGTATGGCTGAAGCCAACAAAGCATTCTCTCACTTCCGTTTCTAA

cDNA: SEQ ID NO: 116

TTAGAAACGGAAGTGAGAGAATGCTTTGTTGGCTTCAGCCATACGGTGTACTTCTTCACGTTTTTTCAACGCACCGCCACGGCCTTCGGACGCATCAATCAACTCGCCTGCCAAACGCAGATCCATGGATTTCTCACCACGTTTGCGGGCCGCGTCGCGAACCCAACGCATTGCCAAAGCCAGACGGCGTGAAGGACGAACTTCAACAGGAACTTGGTAGTTTGCACCACCTACACGGCGGCTTTTCACTTCCACGATAGGTTTGGAGTTTGCAATGGCTTCGTTAAATACTTCGATTGCTGCTTTGCCGGTTTTTTTCTCAATCTGTTCCAACGCACCGTAAACGATACGCTCGGCAACGGATTTTTTACCGTCAATCATCAATACGTTCATGAATTTGGTCAACTCGACGCTACCGAATTTAGGATCTGGCAGTACGTCGCGCTTGGGGACTTCT CTACGTCTTGGCAT

RNA: SEQ ID NO: 117

AUGCCAAGACGUAGAGAAGUCCCCAAGCGCGACGUACUGCCAGAUCCUAAAUUCGGUAGCGUCGAGUUGACCAAAUUCAUGAACGUAUUGAUGAUUGACGGUAAAAAAUCCGUUGCCGAGCGUAUCGUUUACGGUGCGUUGGAACAGAUUGAGAAAAAAACCGGCAAAGCAGCAAUCGAAGUAUUUAACGAAGCCAUUGCAAACUCCAAACCUAUCGUGGAAGUGAAAAGCCGCCGUGUAGGUGGUGCAAACUACCAAGUUCCUGUUGAAGUUCGUCCUUCACGCCGUCUGGCUUUGGCAAUGCGUUGGGUUCGCGACGCGGCCCGCAAACGUGGUGAGAAAUCCAUGGAUCUGCGUUUGGCAGGCGAGUUGAUUGAUGCGUCCGAAGGCCGUGGCGGUGCGUUGAAAAAACGUGAAGAAGUACACCGUAUGGCUGAAGCCAACAAAGCAUUCUCUCACUUCCGUUUCUAA

31. NGO1845: NC_002946.2:c1812043-1811672

DNA (- strand): SEQ ID NO: 118

ATGCCAACTATCAACCAATTGGTACGCAAAGGCCGTCAAAAGCCCGTGTACGTAAACAAAGTGCCCGCACTGGAAGCCTGCCCGCAAAAACGCGGCGTGTGCACCCGTGTATACACGACTACCCCTAGAAAACCTAACTCTGCATTGCGTAAAGTATGTAAAGTCCGCCTGACCAACGGTTTTGAAGTCATTTCATATATCGGCGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTACTGATTCGCGGCGGCCGTGTAAAAGACTTGCCGGGTGTACGTTACCACACTGTACGCGGTTCTTTGGATACTGCAGGTGTTAAAGACCGCAAACAAGCCCGTTCTAAATACGGTGCTAAGCGTCCTAAATAA

cDNA: SEQ ID NO: 119

TTATTTAGGACGCTTAGCACCGTATTTAGAACGGGCTTGTTTGCGGTCTTTAACACCTGCAGTATCCAAAGAACCGCGTACAGTGTGGTAACGTACACCCGGCAAGTCTTTTACACGGCCGCCGCGAATCAGTACGACGCTGTGCTCTTGCAGGTTGTGGCCTTCACCGCCGATATATGAAATGACTTCAAAACCGTTGGTCAGGCGGACTTTACATACTTTACGCAATGCAGAGTTAGGTTTTCTAGGGGTAGTCGTGTATACACGGGTGCACACGCCGCGTTTTTGCGGGCAGGCTTCCAGTGCGGGCACTTTGTTTACGTACACGGGCTTTTGACGGCCTTTGCGTACCAATTGGTTGATAGTTGGCAT

RNA: SEQ ID NO: 120

AUGCCAACUAUCAACCAAUUGGUACGCAAAGGCCGUCAAAAGCCCGUGUACGUAAACAAAGUGCCCGCACUGGAAGCCUGCCCGCAAAAACGCGGCGUGUGCACCCGUGUAUACACGACUACCCCUAGAAAACCUAACUCUGCAUUGCGUAAAGUAUGUAAAGUCCGCCUGACCAACGGUUUUGAAGUCAUUUCAUAUAUCGGCGGUGAAGGCCACAACCUGCAAGAGCACAGCGUCGUACUGAUUCGCGGCGGCCGUGUAAAAGACUUGCCGGGUGUACGUUACCACACUGUACGCGGUUCUUUGGAUACUGCAGGUGUUAAAGACCGCAAACAAGCCCGUUCUAAAUACGGUGCUAAGCGUCCUAAAUAA

32. NGO1890: NC_002946.2:c1857972-1856758

DNA (- strand): SEQ ID NO: 121

ATGGAATGGGCGTTTAACAGTTATTACACCTTGATTGCCGCCACTTTGGTTTTGTTGGTCGGCAAGGTTTTGGTTAAGAAAATCAAAATCTTGCGTGATTTTAACATCCCCGAACCCGTGGCGGGCGGGCTGATTGCCGCGATTATCCTGTTTGCGCTGCACGAGGCGTACGGCGTGAGCTTCAAATTTGAGAAACCGCTGCAAAATGCGTTTATGCTGATTTTCTTCACGTCCATCGGCTTGAGCGCGGATTTTTCCCGTTTGAAGGCGGGCGGTTTGCCGCTGGTGGTTTTTACCGCGATTGTGGGCGGATTTATCTTGGTGCAAAACTTTGTCGGGGTCGGACTGGCTACGGCTTTGGGTTTGGACCCGCTCATCGGTCTGATTACCGGTTCGGTGTCGCTGACGGGCGGACACGGCACGTCAGGTGCGTGGGGACCTAATTTTGAAACGCAATACGGCTTGGTCGGCGCAACCGGTTTGGGTATTGCTTCGGTTACTTTCGGGCTGGTGTTCGGCGGCCTGATCGGAGGGCCGGTTGCGCGCCGCCTGATCAACAAAATGGGCCGCAAACCGGTTGAAAACACAAAACAGGATCAGGACGACAACGCGGACGACGTGTTCGAGCAGGCAAAACGCACCCGCCTGATTACGGCGGAATCTGCCGTTGAAACGCTTGCCATGTTTGCCGCGTGTCTGGCGTTTGCCGAGATTATGGACGGTTTCGACAAAGAATACCTGTTCGACCTGCCCAAATTCGTGTGGTGTCTGTTTGGCGGCGTGGTTATCCGCAACATCCTTACCGCCGCATTCAAGGTCAATATGTTCGACCGTGCCATCGATGTGTTCGGCAATGCTTCGCTTTCGCTTTTCTTGGCAATGGCGTTGCTGAATTTGAAACTGTGGGAGCTGACCGGTTTGGCGGGGTCTGTAACCGTGATTCTTGCAGTACAAACCGCAGTGATGGTTTTGTACGCGACTTTTGTTACCTATGTCTTTATGGGGCGCGACTATGATGCCGCAGTATTGGCTGCCGGCCACTGCGGTTTCGGTTTGGGCGCAACGCCGACGGCGGTGGCAAATATGCAGTCCGTCACGCATACTTTCGGCGCGTCACATAAGGCGTTTTTGATTGTGCCTATGGTCGGCGCGTTCTTTGTCGATTTGATTAATGCCGCGATTCTCACCGGTTTTGTGAATTTCTTTAAAGGCTGA

cDNA: SEQ ID NO: 122

TCAGCCTTTAAAGAAATTCACAAAACCGGTGAGAATCGCGGCATTAATCAAATCGACAAAGAACGCGCCGACCATAGGCACAATCAAAAACGCCTTATGTGACGCGCCGAAAGTATGCGTGACGGACTGCATATTTGCCACCGCCGTCGGCGTTGCGCCCAAACCGAAACCGCAGTGGCCGGCAGCCAATACTGCGGCATCATAGTCGCGCCCCATAAAGACATAGGTAACAAAAGTCGCGTACAAAACCATCACTGCGGTTTGTACTGCAAGAATCACGGTTACAGACCCCGCCAAACCGGTCAGCTCCCACAGTTTCAAATTCAGCAACGCCATTGCCAAGAAAAGCGAAAGCGAAGCATTGCCGAACACATCGATGGCACGGTCGAACATATTGACCTTGAATGCGGCGGTAAGGATGTTGCGGATAACCACGCCGCCAAACAGACACCACACGAATTTGGGCAGGTCGAACAGGTATTCTTTGTCGAAACCGTCCATAATCTCGGCAAACGCCAGACACGCGGCAAACATGGCAAGCGTTTCAACGGCAGATTCCGCCGTAATCAGGCGGGTGCGTTTTGCCTGCTCGAACACGTCGTCCGCGTTGTCGTCCTGATCCTGTTTTGTGTTTTCAACCGGTTTGCGGCCCATTTTGTTGATCAGGCGGCGCGCAACCGGCCCTCCGATCAGGCCGCCGAACACCAGCCCGAAAGTAACCGAAGCAATACCCAAACCGGTTGCGCCGACCAAGCCGTATTGCGTTTCAAAATTAGGTCCCCACGCACCTGACGTGCCGTGTCCGCCCGTCAGCGACACCGAACCGGTAATCAGACCGATGAGCGGGTCCAAACCCAAAGCCGTAGCCAGTCCGACCCCGACAAAGTTTTGCACCAAGATAAATCCGCCCACAATCGCGGTAAAAACCACCAGCGGCAAACCGCCCGCCTTCAAACGGGAAAAATCCGCGCTCAAGCCGATGGACGTGAAGAAAATCAGCATAAACGCATTTTGCAGCGGTTTCTCAAATTTGAAGCTCACGCCGTACGCCTCGTGCAGCGCAAACAGGATAATCGCGGCAATCAGCCCGCCCGCCACGGGTTCGGGGATGTTAAAATCACGCAAGATTTTGATTTTCTTAACCAAAACCTTGCCGACCAACAAAACCAAAGTGGCGGCAATCAAGGTGTAATAACTGTTAAACGCCCATTCCAT

RNA: SEQ ID NO: 123

AUGGAAUGGGCGUUUAACAGUUAUUACACCUUGAUUGCCGCCACUUUGGUUUUGUUGGUCGGCAAGGUUUUGGUUAAGAAAAUCAAAAUCUUGCGUGAUUUUAACAUCCCCGAACCCGUGGCGGGCGGGCUGAUUGCCGCGAUUAUCCUGUUUGCGCUGCACGAGGCGUACGGCGUGAGCUUCAAAUUUGAGAAACCGCUGCAAAAUGCGUUUAUGCUGAUUUUCUUCACGUCCAUCGGCUUGAGCGCGGAUUUUUCCCGUUUGAAGGCGGGCGGUUUGCCGCUGGUGGUUUUUACCGCGAUUGUGGGCGGAUUUAUCUUGGUGCAAAACUUUGUCGGGGUCGGACUGGCUACGGCUUUGGGUUUGGACCCGCUCAUCGGUCUGAUUACCGGUUCGGUGUCGCUGACGGGCGGACACGGCACGUCAGGUGCGUGGGGACCUAAUUUUGAAACGCAAUACGGCUUGGUCGGCGCAACCGGUUUGGGUAUUGCUUCGGUUACUUUCGGGCUGGUGUUCGGCGGCCUGAUCGGAGGGCCGGUUGCGCGCCGCCUGAUCAACAAAAUGGGCCGCAAACCGGUUGAAAACACAAAACAGGAUCAGGACGACAACGCGGACGACGUGUUCGAGCAGGCAAAACGCACCCGCCUGAUUACGGCGGAAUCUGCCGUUGAAACGCUUGCCAUGUUUGCCGCGUGUCUGGCGUUUGCCGAGAUUAUGGACGGUUUCGACAAAGAAUACCUGUUCGACCUGCCCAAAUUCGUGUGGUGUCUGUUUGGCGGCGUGGUUAUCCGCAACAUCCUUACCGCCGCAUUCAAGGUCAAUAUGUUCGACCGUGCCAUCGAUGUGUUCGGCAAUGCUUCGCUUUCGCUUUUCUUGGCAAUGGCGUUGCUGAAUUUGAAACUGUGGGAGCUGACCGGUUUGGCGGGGUCUGUAACCGUGAUUCUUGCAGUACAAACCGCAGUGAUGGUUUUGUACGCGACUUUUGUUACCUAUGUCUUUAUGGGGCGCGACUAUGAUGCCGCAGUAUUGGCUGCCGGCCACUGCGGUUUCGGUUUGGGCGCAACGCCGACGGCGGUGGCAAAUAUGCAGUCCGUCACGCAUACUUUCGGCGCGUCACAUAAGGCGUUUUUGAUUGUGCCUAUGGUCGGCGCGUUCUUUGUCGAUUUGAUUAAUGCCGCGAUUCUCACCGGUUUUGUGAAUUUCUUUAAAGGCUGA

33. Ngo2024: Nc_002946.2:1995172-1995603

DNA (+ strand): SEQ ID NO: 124

ATGAAAACCTTTTCAGCGAAACCCCACGAGGTGAAGCGCGAATGGTTCGTCATCGATGCCCAAGACAAAGTCTTGGGTCGCGTTGCAACCGAAGTCGCCAGCCGTCTGCGTGGCAAACACAAACCTGAATACACCCCCCACGTCGATACCGGCGATTACATCATCGTCATCAATGCGGACAAACTGCGTGTAACCGGTGCCAAATTCGAAGATAAAAAATACTTCCGCCATTCCGGTTTTCCAGGCGGCATCTACGAGCGCACTTTCCGCGAAATGCAAGATCAATTCCCGGGCCGCGCTTTGGAGCAGGCTGTAAAAGGTATGCTGCCCAAAGGTCCGCTGGGTTACGCCATGATTAAAAAACTGAAAGTGTACGCTGGTGCGGAGCATGCCCATGCTGCGCAACAACCCAAAGTTTTGGAACTGAAATAA

cDNA: SEQ ID NO: 125

TTATTTCAGTTCCAAAACTTTGGGTTGTTGCGCAGCATGGGCATGCTCCGCACCAGCGTACACTTTCAGTTTTTTAATCATGGCGTAACCCAGCGGACCTTTGGGCAGCATACCTTTTACAGCCTGCTCCAAAGCGCGGCCCGGGAATTGATCTTGCATTTCGCGGAAAGTGCGCTCGTAGATGCCGCCTGGAAAACCGGAATGGCGGAAGTATTTTTTATCTTCGAATTTGGCACCGGTTACACGCAGTTTGTCCGCATTGATGACGATGATGTAATCGCCGGTATCGACGTGGGGGGTGTATTCAGGTTTGTGTTTGCCACGCAGACGGCTGGCGACTTCGGTTGCAACGCGACCCAAGACTTTGTCTTGGGCATCGATGACGAACCATTCGCGCTTCACCTCGTGGGGTTTCGCTGAAAAGGTTTTCAT

RNA: SEQ ID NO: 126

AUGAAAACCUUUUCAGCGAAACCCCACGAGGUGAAGCGCGAAUGGUUCGUCAUCGAUGCCCAAGACAAAGUCUUGGGUCGCGUUGCAACCGAAGUCGCCAGCCGUCUGCGUGGCAAACACAAACCUGAAUACACCCCCCACGUCGAUACCGGCGAUUACAUCAUCGUCAUCAAUGCGGACAAACUGCGUGUAACCGGUGCCAAAUUCGAAGAUAAAAAAUACUUCCGCCAUUCCGGUUUUCCAGGCGGCAUCUACGAGCGCACUUUCCGCGAAAUGCAAGAUCAAUUCCCGGGCCGCGCUUUGGAGCAGGCUGUAAAAGGUAUGCUGCCCAAAGGUCCGCUGGGUUACGCCAUGAUUAAAAAACUGAAAGUGUACGCUGGUGCGGAGCAUGCCCAUGCUGCGCAACAACCCAAAGUUUUGGAACUGAAAUAA

34. NGO2098: NC_002946.2:c2078739-2077519

DNA (- strand): SEQ ID NO: 127

ATGACCCTGTTTTGCGAACAAGTCCCCTACCCCCGCCTTGCCGAAGAATTCGGCACGCCGCTTTATGTGTACAGCCAATCCGCGCTGACCGGAGCATTTGAAAACTATCAAACCGCCTTTGCCGCTTTGAACCCGCTTGTCTGCTACGCCGTCAAGGCAAACGGCAACCTGAGCATTATCAAACACTTTGCTTCTTTGGGCAGCGGTTTTGACATTGTGTCGGGCGGCGAATTGGCACGCGTTTTGGCGGCAGGCGGCGATGCGGCGAAAACGATTTTTTCCGGCGTAGGCAAAAGCGAGGCGGAAATCGAGTTCGCGCTGAATGCCGGCGTAAAATGCTTCAATATGGAAAGCATCCCCGAAATCGACCGCATTCAGAAAATTGCCGCGCGTTTGGGCAAAACCGCGCCCGTCTCCCTGCGCGTCAATCCCGATGTCGATGCAAAAACCCATCCCTACATCTCCACAGGTCTGAAAGCCAACAAATTCGGCATCGCCTACGCCGACGCGCTCGAAGCCTACCGCCATGCCGCACAACAGCCCAATTTGAAAATCATCGGCATCGACTGCCACATCGGTTCGCAACTGACCGACTTAAGCCCACTGGTCGAAGCCTGCGAACGCATTTTGATTTTGGTTGACGCTCTTGCCGCCGAAGGCATTGTTTTGGAACATTTGGACTTAGGCGGCGGCGTCGGCATTGTTTACAAAGACGAAGGCGTCCCCGATTTGGGTGCGTATGCCCGAGCGGTTCAAAAACTGATGGGGACACGCCGTCTGAAACTCATTCTTGAGCCAGGCCGCAGCTTGGTCGGCAACGCAGGTGCATTGCTGACGCGCGTCGAATTTGTCAAACACGGTGAAGAGAAAAACTTTGTGATGGTCGATGCGGCGATGAACGATTTGATGCGCCCAGCCCTATACGATGCCTACCACCACATCGAAGCGGTTGAAACCAAAAACATTGAGCCTCTGACCGCCAACATCGTCGGCCCGATTTGTGAAACCGGCGACTTCCTCGGCAAAGACCGCACCATCGCCTGCGAAGAAGGCGATTTGCTGCTTATCCGCAGCGCGGGCGCATACGGGGCCAGTATGGCTAGCAATTACAACACGCGCAACCGTGCGGCGGAGGTGTTGGTTGACGGCGGCGGATACAAACTCATCCGCCGGCGCGAAACCTTGGAACAGCAAATGGCAAACGAACTCGCCTGCCTATAA

cDNA: SEQ ID NO: 128

TTATAGGCAGGCGAGTTCGTTTGCCATTTGCTGTTCCAAGGTTTCGCGCCGGCGGATGAGTTTGTATCCGCCGCCGTCAACCAACACCTCCGCCGCACGGTTGCGCGTGTTGTAATTGCTAGCCATACTGGCCCCGTATGCGCCCGCGCTGCGGATAAGCAGCAAATCGCCTTCTTCGCAGGCGATGGTGCGGTCTTTGCCGAGGAAGTCGCCGGTTTCACAAATCGGGCCGACGATGTTGGCGGTCAGAGGCTCAATGTTTTTGGTTTCAACCGCTTCGATGTGGTGGTAGGCATCGTATAGGGCTGGGCGCATCAAATCGTTCATCGCCGCATCGACCATCACAAAGTTTTTCTCTTCACCGTGTTTGACAAATTCGACGCGCGTCAGCAATGCACCTGCGTTGCCGACCAAGCTGCGGCCTGGCTCAAGAATGAGTTTCAGACGGCGTGTCCCCATCAGTTTTTGAACCGCTCGGGCATACGCACCCAAATCGGGGACGCCTTCGTCTTTGTAAACAATGCCGACGCCGCCGCCTAAGTCCAAATGTTCCAAAACAATGCCTTCGGCGGCAAGAGCGTCAACCAAAATCAAAATGCGTTCGCAGGCTTCGACCAGTGGGCTTAAGTCGGTCAGTTGCGAACCGATGTGGCAGTCGATGCCGATGATTTTCAAATTGGGCTGTTGTGCGGCATGGCGGTAGGCTTCGAGCGCGTCGGCGTAGGCGATGCCGAATTTGTTGGCTTTCAGACCTGTGGAGATGTAGGGATGGGTTTTTGCATCGACATCGGGATTGACGCGCAGGGAGACGGGCGCGGTTTTGCCCAAACGCGCGGCAATTTTCTGAATGCGGTCGATTTCGGGGATGCTTTCCATATTGAAGCATTTTACGCCGGCATTCAGCGCGAACTCGATTTCCGCCTCGCTTTTGCCTACGCCGGAAAAAATCGTTTTCGCCGCATCGCCGCCTGCCGCCAAAACGCGTGCCAATTCGCCGCCCGACACAATGTCAAAACCGCTGCCCAAAGAAGCAAAGTGTTTGATAATGCTCAGGTTGCCGTTTGCCTTGACGGCGTAGCAGACAAGCGGGTTCAAAGCGGCAAAGGCGGTTTGATAGTTTTCAAATGCTCCGGTCAGCGCGGATTGGCTGTACACATAAAGCGGCGTGCCGAATTCTTCGGCAAGGCGGGGGTAGGGGACTTGTTCGCAAAACAGGGTCAT

RNA: SEQ ID NO: 129

AUGACCCUGUUUUGCGAACAAGUCCCCUACCCCCGCCUUGCCGAAGAAUUCGGCACGCCGCUUUAUGUGUACAGCCAAUCCGCGCUGACCGGAGCAUUUGAAAACUAUCAAACCGCCUUUGCCGCUUUGAACCCGCUUGUCUGCUACGCCGUCAAGGCAAACGGCAACCUGAGCAUUAUCAAACACUUUGCUUCUUUGGGCAGCGGUUUUGACAUUGUGUCGGGCGGCGAAUUGGCACGCGUUUUGGCGGCAGGCGGCGAUGCGGCGAAAACGAUUUUUUCCGGCGUAGGCAAAAGCGAGGCGGAAAUCGAGUUCGCGCUGAAUGCCGGCGUAAAAUGCUUCAAUAUGGAAAGCAUCCCCGAAAUCGACCGCAUUCAGAAAAUUGCCGCGCGUUUGGGCAAAACCGCGCCCGUCUCCCUGCGCGUCAAUCCCGAUGUCGAUGCAAAAACCCAUCCCUACAUCUCCACAGGUCUGAAAGCCAACAAAUUCGGCAUCGCCUACGCCGACGCGCUCGAAGCCUACCGCCAUGCCGCACAACAGCCCAAUUUGAAAAUCAUCGGCAUCGACUGCCACAUCGGUUCGCAACUGACCGACUUAAGCCCACUGGUCGAAGCCUGCGAACGCAUUUUGAUUUUGGUUGACGCUCUUGCCGCCGAAGGCAUUGUUUUGGAACAUUUGGACUUAGGCGGCGGCGUCGGCAUUGUUUACAAAGACGAAGGCGUCCCCGAUUUGGGUGCGUAUGCCCGAGCGGUUCAAAAACUGAUGGGGACACGCCGUCUGAAACUCAUUCUUGAGCCAGGCCGCAGCUUGGUCGGCAACGCAGGUGCAUUGCUGACGCGCGUCGAAUUUGUCAAACACGGUGAAGAGAAAAACUUUGUGAUGGUCGAUGCGGCGAUGAACGAUUUGAUGCGCCCAGCCCUAUACGAUGCCUACCACCACAUCGAAGCGGUUGAAACCAAAAACAUUGAGCCUCUGACCGCCAACAUCGUCGGCCCGAUUUGUGAAACCGGCGACUUCCUCGGCAAAGACCGCACCAUCGCCUGCGAAGAAGGCGAUUUGCUGCUUAUCCGCAGCGCGGGCGCAUACGGGGCCAGUAUGGCUAGCAAUUACAACACGCGCAACCGUGCGGCGGAGGUGUUGGUUGACGGCGGCGGAUACAAACUCAUCCGCCGGCGCGAAACCUUGGAACAGCAAAUGGCAAACGAACUCGCCUGCCUAUAA

35. Ngo2100: Nc_002946.2:2078991-2079314

DNA (+ strand): SEQ ID NO: 130

ATGATGACCGAAAGCGAGTTTATCCGCGCGAGCGAAGCATTATTTGAACACATCGAAGACCAAATCGACGAAAACGGCTGGGATTTCGACTGCCGGTTTGCCGGAAACGTCCTGACCATCGAAGCCGGAGACGGCACGCAAATCATCGTCAACCGCCACACGCCCAACCAAGAATTGTGGATTGCCGCAAAAAGCGGCGGCTACCATTTCGCCGAACAAAACGGCAAATGGCTGGCAACGCGCGACAGCCGCGATTTTTACGACGTTTTAAACGAAGCCCTGAGCGCGGCTTCGGGCGAAGCGGTTGAGATTGCCGAATTGTGA

cDNA: SEQ ID NO: 131

TCACAATTCGGCAATCTCAACCGCTTCGCCCGAAGCCGCGCTCAGGGCTTCGTTTAAAACGTCGTAAAAATCGCGGCTGTCGCGCGTTGCCAGCCATTTGCCGTTTTGTTCGGCGAAATGGTAGCCGCCGCTTTTTGCGGCAATCCACAATTCTTGGTTGGGCGTGTGGCGGTTGACGATGATTTGCGTGCCGTCTCCGGCTTCGATGGTCAGGACGTTTCCGGCAAACCGGCAGTCGAAATCCCAGCCGTTTTCGTCGATTTGGTCTTCGATGTGTTCAAATAATGCTTCGCTCGCGCGGATAAACTCGCTTTCGGTCATCAT

RNA: SEQ ID NO: 132

AUGAUGACCGAAAGCGAGUUUAUCCGCGCGAGCGAAGCAUUAUUUGAACACAUCGAAGACCAAAUCGACGAAAACGGCUGGGAUUUCGACUGCCGGUUUGCCGGAAACGUCCUGACCAUCGAAGCCGGAGACGGCACGCAAAUCAUCGUCAACCGCCACACGCCCAACCAAGAAUUGUGGAUUGCCGCAAAAAGCGGCGGCUACCAUUUCGCCGAACAAAACGGCAAAUGGCUGGCAACGCGCGACAGCCGCGAUUUUUACGACGUUUUAAACGAAGCCCUGAGCGCGGCUUCGGGCGAAGCGGUUGAGAUUGCCGAAUUGUGA

36. NGO2164: NC_002946.2:c2141372-2139807

DNA (- strand): SEQ ID NO: 133

ATGACCCAAGACAAAATCCTCATCCTCGACTTCGGTTCTCAAGTTACCCGGCTGATTGCCCGCCGCGTGCGCGAAGCCCACGTTTACTGCGAACTGCATTCCTTCGATATGCCTTTGGACGAAATCAAAGCCTTCAACCCCAAAGGCATCATCCTTTCCGGCGGCCCTAATTCTGTTTACGAATCCGACTATCAAGCCGATACCGGTATTTTTGATTTGGGCATTCCGGTTTTGGGCATCTGCTACGGCATGCAGTTTATGGCGCACCACTTGGGTGGCGAAGTGCAGCCCGGCAACCAGCGCGAATTCGGTTACGCGCAAGTCAAAACCATCGACAGCGGACTGACACGCGGCATTCAAGACGACGCGCCCAACACACTCGACGTATGGATGAGCCACGGCGACAAAGTGTCCAAACTGCCCGACGGTTTCGCCGTCATCGGCGATACCCCGTCCTGCCCGATTGCAATGATGGAAAACGCCGAAAAACAATTCTACGGCATCCAGTTCCACCCCGAAGTTACCCACACCAAACAAGGCCGCGCCCTGTTGAACCGCTTTGTCTTGGATATTTGCGGCGCGCAACCGGGCTGGACGATGCCCAACTACATCGAAGAAGCCGTTGCCAAAATCCGCGAACAAGTCGGCAGCGACGAAGTGATTTTAGGTCTGTCCGGCGGCGTGGACTCTTCCGTAGCCGCCGCGCTGATTCACCGCGCCATCGGCGACCAACTGACCTGCGTGTTCGTCGATCACGGTTTGTTGCGCCTGAACGAAGGCAAAATGGTGATGGATATGTTCGCCCGCAACTTGGGTGTGAAAGTGATACACGTCGATGCCGAAGGGCAGTTTATGGCGAAACTCGCCGGCGTGACCGACCCTGAGAAAAAACGCAAAATCATCGGCGCGGAATTTATCGAAGTATTTGATGCCGAAGAGAAAAAACTCACCAACGCCAAATGGCTGGCGCAAGGCACGATTTACCCCGACGTAATCGAATCCGCCGGTGCGAAAACCAAAAAAGCCCACGCCATCAAATCCCACCACAACGTCGGCGGCCTGCCTGAAAATATGAAGCTCAAACTGCTTGAGCCCTTGCGCGACTTGTTCAAAGACGAAGTGCGCGAGTTGGGCGTGGCTTTGGGCCTGCCGCGCGAAATGGTGTACCGCCACCCCTTCCCGGGCCCCGGTTTGGGTGTGCGCATCTTGGGCGAAGTGAAAAAAGAATACGCCGACTTGCTGCGTCAGGCGGACGATATTTTCATCCAAGAATTACGCAATACTACCGACGAAAACGGCACGTCTTGGTATGACCTGACCAGCCAGGCATTTGCCGTATTCCTGCCCGTCAAATCCGTCGGCGTGATGGGCGACGGCCGCACTTACGACTACGTCGTCGCACTGCGCGCAGTCATCACCAGCGACTTTATGACTGCACACTGGGCAGAGCTGCCATACTCACTGCTCGGCCGCGTGTCCAACCGCATCATCAACGAAGTCAAAGGCATCAACCGCGTGGTGTACGATGTCAGCGGCAAACCGCCCGCCACCATCGAGTGGGAATAA

cDNA: SEQ ID NO: 134

TTATTCCCACTCGATGGTGGCGGGCGGTTTGCCGCTGACATCGTACACCACGCGGTTGATGCCTTTGACTTCGTTGATGATGCGGTTGGACACGCGGCCGAGCAGTGAGTATGGCAGCTCTGCCCAGTGTGCAGTCATAAAGTCGCTGGTGATGACTGCGCGCAGTGCGACGACGTAGTCGTAAGTGCGGCCGTCGCCCATCACGCCGACGGATTTGACGGGCAGGAATACGGCAAATGCCTGGCTGGTCAGGTCATACCAAGACGTGCCGTTTTCGTCGGTAGTATTGCGTAATTCTTGGATGAAAATATCGTCCGCCTGACGCAGCAAGTCGGCGTATTCTTTTTTCACTTCGCCCAAGATGCGCACACCCAAACCGGGGCCCGGGAAGGGGTGGCGGTACACCATTTCGCGCGGCAGGCCCAAAGCCACGCCCAACTCGCGCACTTCGTCTTTGAACAAGTCGCGCAAGGGCTCAAGCAGTTTGAGCTTCATATTTTCAGGCAGGCCGCCGACGTTGTGGTGGGATTTGATGGCGTGGGCTTTTTTGGTTTTCGCACCGGCGGATTCGATTACGTCGGGGTAAATCGTGCCTTGCGCCAGCCATTTGGCGTTGGTGAGTTTTTTCTCTTCGGCATCAAATACTTCGATAAATTCCGCGCCGATGATTTTGCGTTTTTTCTCAGGGTCGGTCACGCCGGCGAGTTTCGCCATAAACTGCCCTTCGGCATCGACGTGTATCACTTTCACACCCAAGTTGCGGGCGAACATATCCATCACCATTTTGCCTTCGTTCAGGCGCAACAAACCGTGATCGACGAACACGCAGGTCAGTTGGTCGCCGATGGCGCGGTGAATCAGCGCGGCGGCTACGGAAGAGTCCACGCCGCCGGACAGACCTAAAATCACTTCGTCGCTGCCGACTTGTTCGCGGATTTTGGCAACGGCTTCTTCGATGTAGTTGGGCATCGTCCAGCCCGGTTGCGCGCCGCAAATATCCAAGACAAAGCGGTTCAACAGGGCGCGGCCTTGTTTGGTGTGGGTAACTTCGGGGTGGAACTGGATGCCGTAGAATTGTTTTTCGGCGTTTTCCATCATTGCAATCGGGCAGGACGGGGTATCGCCGATGACGGCGAAACCGTCGGGCAGTTTGGACACTTTGTCGCCGTGGCTCATCCATACGTCGAGTGTGTTGGGCGCGTCGTCTTGAATGCCGCGTGTCAGTCCGCTGTCGATGGTTTTGACTTGCGCGTAACCGAATTCGCGCTGGTTGCCGGGCTGCACTTCGCCACCCAAGTGGTGCGCCATAAACTGCATGCCGTAGCAGATGCCCAAAACCGGAATGCCCAAATCAAAAATACCGGTATCGGCTTGATAGTCGGATTCGTAAACAGAATTAGGGCCGCCGGAAAGGATGATGCCTTTGGGGTTGAAGGCTTTGATTTCGTCCAAAGGCATATCGAAGGAATGCAGTTCGCAGTAAACGTGGGCTTCGCGCACGCGGCGGGCAATCAGCCGGGTAACTTGAGAACCGAAGTCGAGGATGAGGATTTTGTCTTGGGTCAT

RNA: SEQ ID NO: 135

AUGACCCAAGACAAAAUCCUCAUCCUCGACUUCGGUUCUCAAGUUACCCGGCUGAUUGCCCGCCGCGUGCGCGAAGCCCACGUUUACUGCGAACUGCAUUCCUUCGAUAUGCCUUUGGACGAAAUCAAAGCCUUCAACCCCAAAGGCAUCAUCCUUUCCGGCGGCCCUAAUUCUGUUUACGAAUCCGACUAUCAAGCCGAUACCGGUAUUUUUGAUUUGGGCAUUCCGGUUUUGGGCAUCUGCUACGGCAUGCAGUUUAUGGCGCACCACUUGGGUGGCGAAGUGCAGCCCGGCAACCAGCGCGAAUUCGGUUACGCGCAAGUCAAAACCAUCGACAGCGGACUGACACGCGGCAUUCAAGACGACGCGCCCAACACACUCGACGUAUGGAUGAGCCACGGCGACAAAGUGUCCAAACUGCCCGACGGUUUCGCCGUCAUCGGCGAUACCCCGUCCUGCCCGAUUGCAAUGAUGGAAAACGCCGAAAAACAAUUCUACGGCAUCCAGUUCCACCCCGAAGUUACCCACACCAAACAAGGCCGCGCCCUGUUGAACCGCUUUGUCUUGGAUAUUUGCGGCGCGCAACCGGGCUGGACGAUGCCCAACUACAUCGAAGAAGCCGUUGCCAAAAUCCGCGAACAAGUCGGCAGCGACGAAGUGAUUUUAGGUCUGUCCGGCGGCGUGGACUCUUCCGUAGCCGCCGCGCUGAUUCACCGCGCCAUCGGCGACCAACUGACCUGCGUGUUCGUCGAUCACGGUUUGUUGCGCCUGAACGAAGGCAAAAUGGUGAUGGAUAUGUUCGCCCGCAACUUGGGUGUGAAAGUGAUACACGUCGAUGCCGAAGGGCAGUUUAUGGCGAAACUCGCCGGCGUGACCGACCCUGAGAAAAAACGCAAAAUCAUCGGCGCGGAAUUUAUCGAAGUAUUUGAUGCCGAAGAGAAAAAACUCACCAACGCCAAAUGGCUGGCGCAAGGCACGAUUUACCCCGACGUAAUCGAAUCCGCCGGUGCGAAAACCAAAAAAGCCCACGCCAUCAAAUCCCACCACAACGUCGGCGGCCUGCCUGAAAAUAUGAAGCUCAAACUGCUUGAGCCCUUGCGCGACUUGUUCAAAGACGAAGUGCGCGAGUUGGGCGUGGCUUUGGGCCUGCCGCGCGAAAUGGUGUACCGCCACCCCUUCCCGGGCCCCGGUUUGGGUGUGCGCAUCUUGGGCGAAGUGAAAAAAGAAUACGCCGACUUGCUGCGUCAGGCGGACGAUAUUUUCAUCCAAGAAUUACGCAAUACUACCGACGAAAACGGCACGUCUUGGUAUGACCUGACCAGCCAGGCAUUUGCCGUAUUCCUGCCCGUCAAAUCCGUCGGCGUGAUGGGCGACGGCCGCACUUACGACUACGUCGUCGCACUGCGCGCAGUCAUCACCAGCGACUUUAUGACUGCACACUGGGCAGAGCUGCCAUACUCACUGCUCGGCCGCGUGUCCAACCGCAUCAUCAACGAAGUCAAAGGCAUCAACCGCGUGGUGUACGAUGUCAGCGGCAAACCGCCCGCCACCAUCGAGUGGGAAUAA

37. NGO2173: NC_002946.2:c2149065-2148886

DNA (- strand): SEQ ID NO: 136

ATGGCCGTTCAACAAAACAAAAAATCCCCTTCCAAACGCGGTATGCACCGTTCGCACGACGCACTGACCGCGCCCGCACTGTTTGTCGACAGCACAACCGGCGAAGTACACCGCCCGCACCACATCTCCCCCAACGGTATGTACCGCGGCCGCAAAGTGGTCAAAGCC AAAGGCGAATAA

cDNA: SEQ ID NO: 137

TTATTCGCCTTTGGCTTTGACCACTTTGCGGCCGCGGTACATACCGTTGGGGGAGATGTGGTGCGGGCGGTGTACTTCGCCGGTTGTGCTGTCGACAAACAGTGCGGGCGCGGTCAGTGCGTCGTGCGAACGGTGCATACCGCGTTTGGAAGGGGATTTTTTGTTTTGTTG AACGGCCAT

RNA: SEQ ID NO: 138

AUGGCCGUUCAACAAAACAAAAAAUCCCCUUCCAAACGCGGUAUGCACCGUUCGCACGACGCACUGACCGCGCCCGCACUGUUUGUCGACAGCACAACCGGCGAAGUACACCGCCCGCACCACAUCUCCCCCAACGGUAUGUACCGCGGCCGCAAAGUGGUCAAA GCCAAAGGCGAAUAA

38. NGO2174: NC_002946.2:c2149599-2149099

DNA (- strand): SEQ ID NO: 139

ATGTCAGACCCTAATTTGATTGACCCGGAAATTTTTGCCGCCGAAAGGCAGAACCTGCAAGGCAGTTTTCTGCTGGAAGAATTGGACGAGCGAGTCAGTTTGCACGATTATCCCGCCGACAGGCGGAACAAAATATCGTTTACACTGACCGGCGGTCGCGACCGGCTGCAACGCCTGTTCCTCGACCTGAACGTCAAAGCCGATATGCCCCTGATTTGCCAGAGATGTATCAAACCCATGCCGTTCATGCTCGATGAAAGCAGCCGTATCATCCTGTTTTCCGACGAAGAGTCCTTGGACGAATCCATGCTTGCCGACGAAGAACTCGAAGGCATACTGATTGAAAAAGAACTCGACGTGCGCGCATTGGTAGAAGACCAAATCCTGATGTCCCTGCCCTTTTCGCCGCGACACGGACACTGCGGCAATACCCTTCCGGAATCCGCCAACCAAGACAAACCCAACCCCTTTGCTGTTTTGGCGGGTTTGAAAAGCAGTTAA

cDNA: SEQ ID NO: 140

TTAACTGCTTTTCAAACCCGCCAAAACAGCAAAGGGGTTGGGTTTGTCTTGGTTGGCGGATTCCGGAAGGGTATTGCCGCAGTGTCCGTGTCGCGGCGAAAAGGGCAGGGACATCAGGATTTGGTCTTCTACCAATGCGCGCACGTCGAGTTCTTTTTCAATCAGTATGCCTTCGAGTTCTTCGTCGGCAAGCATGGATTCGTCCAAGGACTCTTCGTCGGAAAACAGGATGATACGGCTGCTTTCATCGAGCATGAACGGCATGGGTTTGATACATCTCTGGCAAATCAGGGGCATATCGGCTTTGACGTTCAGGTCGAGGAACAGGCGTTGCAGCCGGTCGCGACCGCCGGTCAGTGTAAACGATATTTTGTTCCGCCTGTCGGCGGGATAATCGTGCAAACTGACTCGCTCGTCCAATTCTTCCAGCAGAAAACTGCCTTGCAGGTTCTGCCTTTCGGCGGCAAAAATTTCCGGGTCAATCAAATTAGGGTCTGACAT

RNA: SEQ ID NO: 141

AUGUCAGACCCUAAUUUGAUUGACCCGGAAAUUUUUGCCGCCGAAAGGCAGAACCUGCAAGGCAGUUUUCUGCUGGAAGAAUUGGACGAGCGAGUCAGUUUGCACGAUUAUCCCGCCGACAGGCGGAACAAAAUAUCGUUUACACUGACCGGCGGUCGCGACCGGCUGCAACGCCUGUUCCUCGACCUGAACGUCAAAGCCGAUAUGCCCCUGAUUUGCCAGAGAUGUAUCAAACCCAUGCCGUUCAUGCUCGAUGAAAGCAGCCGUAUCAUCCUGUUUUCCGACGAAGAGUCCUUGGACGAAUCCAUGCUUGCCGACGAAGAACUCGAAGGCAUACUGAUUGAAAAAGAACUCGACGUGCGCGCAUUGGUAGAAGACCAAAUCCUGAUGUCCCUGCCCUUUUCGCCGCGACACGGACACUGCGGCAAUACCCUUCCGGAAUCCGCCAACCAAGACAAACCCAACCCCUUUGCUGUUUUGGCGGGUUUGAAAA GCAGUUAA

SEQUENCES FOR TRNA 1. NGO_t01: NC_002946.2:c14067-13982

DNA (- strand): SEQ ID NO: 142

GCCGACATGGTGAAATTGGTAGACACGCTATCTTGAGGGGGTAGTGGCCGTAGGCTGTGCGAGTTCAAATCTCGCTGTCGGCACCA

cDNA: SEQ ID NO: 143

TGGTGCCGACAGCGAGATTTGAACTCGCACAGCCTACGGCCACTACCCCCTCAAGATAGCGTGTCTACCAATTTCACCATGTCGGC

RNA: SEQ ID NO: 144

GCCGACAUGGUGAAAUUGGUAGACACGCUAUCUUGAGGGGGUAGUGGCCGUAGGCUGUGCGAGUUCAAAUCUCGCUGUCGGCACCA

2. NGO_t12: NC_002946.2:454725-454812

DNA (+ strand): SEQ ID NO: 145

GGAAGCGTGGCAGAGCGGTTTAATGCAACGGTCTTGAAAACCGTCGAGGGTTGATAGCCCTCCGTGAGTTCGAATCTCACCGCTTCCG

cDNA: SEQ ID NO: 146

CGGAAGCGGTGAGATTCGAACTCACGGAGGGCTATCAACCCTCGACGGTTTTCAAGACCGTTGCATTAAACCGCTCTGCCACGCTTCC

RNA: SEQ ID NO: 147

GGAAGCGUGGCAGAGCGGUUUAAUGCAACGGUCUUGAAAACCGUCGAGGGUUGAUAGCCCUCCGUGAGUUCGAAUCUCACCGCUUCCG

3. NGO_t14: NC_002946.2:793319-793402

DNA (+ strand): SEQ ID NO: 148

GCCCGGGTGGCGGAATTGGTAGACGCGCCAGCTTCAGGTGCTGGTATCCTCACGGGTATGGAAGTTCGAGTCTTCTCCCGGGCA

cDNA: SEQ ID NO: 149

TGCCCGGGAGAAGACTCGAACTTCCATACCCGTGAGGATACCAGCACCTGAAGCTGGCGCGTCTACCAATTCCGCCACCCGGGC

RNA: SEQ ID NO: 150

GCCCGGGUGGCGGAAUUGGUAGACGCGCCAGCUUCAGGUGCUGGUAUCCUCACGGGUAUGGAAGUUCGAGUCUUCUCCCGGGCA

4. NGO_t15: NC_002946.2:793444-793531

DNA (+ strand): SEQ ID NO: 151

AGAGAGGTGGATGAGTGGTTTAAGTCGCACGCCTGGAAAGCGTGTATACGTGAATAGCGTATCGAGGGTTCGAATCCCTTCCTCTCTG

cDNA: SEQ ID NO: 152

CAGAGAGGAAGGGATTCGAACCCTCGATACGCTATTCACGTATACACGCTTTCCAGGCGTGCGACTTAAACCACTCATCCACCTCTCT

RNA: SEQ ID NO: 153

AGAGAGGUGGAUGAGUGGUUUAAGUCGCACGCCUGGAAAGCGUGUAUACGUGAAUAGCGUAUCGAGGGUUCGAAUCCCUUCCUCUCUG

5. NGO_t37: NC_002946.2:cl629552-1629481

DNA (- strand): SEQ ID NO: 228

CTCGCCATAGTTCAACGGATAGAACGTATGCCTCCTAAGCGTAAAATACAGGTTCGATTCCTGTTGGCGAGG

cDNA: SEQ ID NO: 229

CCTCGCCAACAGGAATCGAACCTGTATTTTACGCTTAGGAGGCATACGTTCTATCCGTTGAACTATGGCGAG

RNA: SEQ ID NO: 230

CUCGCCAUAGUUCAACGGAUAGAACGUAUGCCUCCUAAGCGUAAAAUACAGGUUCGAUUCCUGUUGGCGAGG

ANNEX C Expected Exemplary RNA Markers of N. Meningitides PorBNC_003112.2:2157529-2158524 Neisseria Meningitides MC58

DNA(+)strand: SEQ ID NO: 154

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCAGCAATGGCTGACGTTACCCTGTACGGCACCATCAAAGCCGGCGTAGAAACTTCCCGCTCTGTATTTCACCAGAACGGCCAAGTTACTGAAGTTACAACCGCTACCGGCATCGTTGATTTGGGTTCGAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGTAACGGCCTGAAAGCCATTTGGCAGGTTGAGCAAAAAGCATCTATCGCCGGTACTGACTCCGGTTGGGGCAACCGCCAATCCTTCATCGGCTTGAAAGGCGGCTTCGGTAAATTGCGCGTCGGTCGTTTGAACAGCGTCCTGAAAGACACCGGCGACATCAATCCTTGGGATAGCAAAAGCGACTATTTGGGTGTAAACAAAATTGCCGAACCCGAGGCACGCCTCATTTCCGTACGCTACGATTCTCCCGAATTTGCCGGCCTCAGCGGCAGCGTACAATACGCGCTTAACGACAATGCAGGCAGACATAACAGCGAATCTTACCACGCCGGCTTCAACTACAAAAACGGTGGCTTCTTCGTGCAATATGGCGGTGCCTATAAAAGACATCATCAAGTGCAAGAGGGCTTGAATATTGAGAAATACCAGATTCACCGTTTGGTCAGCGGTTACGACAATGATGCCCTGTACGCTTCCGTAGCCGTACAGCAACAAGACGCGAAACTGACTGATGCTTCCAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCTTGGCATACCGCTTCGGCAACGTAACGCCCCGAGTTTCTTACGCCCACGGCTTCAAAGGTTTGGTTGATGATGCAGACATAGGCAACGAATACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGTTGGTTGCAAGAAGGCAAAGGCGAAAACAAATTCGTAGCGACTGCCGGCGGTGTCGGTCTGCGCCACA AATTCTAA

cDNA: SEQ ID NO: 155

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCAGCAATGGCTGACGTTACCCTGTACGGCACCATCAAAGCCGGCGTAGAAACTTCCCGCTCTGTATTTCACCAGAACGGCCAAGTTACTGAAGTTACAACCGCTACCGGCATCGTTGATTTGGGTTCGAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGTAACGGCCTGAAAGCCATTTGGCAGGTTGAGCAAAAAGCATCTATCGCCGGTACTGACTCCGGTTGGGGCAACCGCCAATCCTTCATCGGCTTGAAAGGCGGCTTCGGTAAATTGCGCGTCGGTCGTTTGAACAGCGTCCTGAAAGACACCGGCGACATCAATCCTTGGGATAGCAAAAGCGACTATTTGGGTGTAAACAAAATTGCCGAACCCGAGGCACGCCTCATTTCCGTACGCTACGATTCTCCCGAATTTGCCGGCCTCAGCGGCAGCGTACAATACGCGCTTAACGACAATGCAGGCAGACATAACAGCGAATCTTACCACGCCGGCTTCAACTACAAAAACGGTGGCTTCTTCGTGCAATATGGCGGTGCCTATAAAAGACATCATCAAGTGCAAGAGGGCTTGAATATTGAGAAATACCAGATTCACCGTTTGGTCAGCGGTTACGACAATGATGCCCTGTACGCTTCCGTAGCCGTACAGCAACAAGACGCGAAACTGACTGATGCTTCCAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCTTGGCATACCGCTTCGGCAACGTAACGCCCCGAGTTTCTTACGCCCACGGCTTCAAAGGTTTGGTTGATGATGCAGACATAGGCAACGAATACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGTTGGTTGCAAGAAGGCAAAGGCGAAAACAAATTCGTAGCGACTGCCGGCGGTGTCGGTCTGCGCCACA AATTCTAA

RNA: SEQ ID NO: 156

UUAGAAUUUGUGGCGCAGACCGACACCGCCGGCAGUCGCUACGAAUUUGUUUUCGCCUUUGCCUUCUUGCAACCAACCGGCAGAAACCAAGGCAGAAGUGCGUUUGGAGAAGUCGUAUUCCGCACCGACAACCACUUGGUCGUAUUCGUUGCCUAUGUCUGCAUCAUCAACCAAACCUUUGAAGCCGUGGGCGUAAGAAACUCGGGGCGUUACGUUGCCGAAGCGGUAUGCCAAGGUAGCGGCAACUUCGGUUUGAGAGUUGUGCGAAUUGGAAGCAUCAGUCAGUUUCGCGUCUUGUUGCUGUACGGCUACGGAAGCGUACAGGGCAUCAUUGUCGUAACCGCUGACCAAACGGUGAAUCUGGUAUUUCUCAAUAUUCAAGCCCUCUUGCACUUGAUGAUGUCUUUUAUAGGCACCGCCAUAUUGCACGAAGAAGCCACCGUUUUUGUAGUUGAAGCCGGCGUGGUAAGAUUCGCUGUUAUGUCUGCCUGCAUUGUCGUUAAGCGCGUAUUGUACGCUGCCGCUGAGGCCGGCAAAUUCGGGAGAAUCGUAGCGUACGGAAAUGAGGCGUGCCUCGGGUUCGGCAAUUUUGUUUACACCCAAAUAGUCGCUUUUGCUAUCCCAAGGAUUGAUGUCGCCGGUGUCUUUCAGGACGCUGUUCAAACGACCGACGCGCAAUUUACCGAAGCCGCCUUUCAAGCCGAUGAAGGAUUGGCGGUUGCCCCAACCGGAGUCAGUACCGGCGAUAGAUGCUUUUUGCUCAACCUGCCAAAUGGCUUUCAGGCCGUUACCGAGGUCUUCUUGGCCUUUGAAGCCGAUUUUCGAACCCAAAUCAACGAUGCCGGUAGCGGUUGUAACUUCAGUAACUUGGCCGUUCUGGUGAAAUACAGAGCGGGAAGUUUCUACGCCGGCUUUGAUGGUGCCGUACAGGGUAACGUCAGCCAUUGCUGCAACAGGAAGGGCUGCCAAAGUCAGGGCAAUCAGGGAUUUUUUCAU

rpmB >NC_003112.2:332567-332800 Neisseria Meningitidis MC58 - on the(-)strand

DNA (+)strand SEQ ID NO: 157

TTAAGCTTCGCCGCGAGCACGCAAATCAGCCAATACGACATCAATGCCTACTTTGTCGATGGTACGCAGTGCAGCGTTGGAAACGCGCAGGCGAACCCAGCGGTTTTCACTTTCTACCCAAAAACGACGTGATTGCAAGTTGGGCAAAAAACGGCGTTTGGTTTTGTTGTTGGCGTGCGATACGTTGTTGCCGGACATCGGGCGTTTGCCGGTCACTTTGCAAACTCGTGCCAT

Cdna SEQ ID NO: 158

ATGGCACGAGTTTGCAAAGTGACCGGCAAACGCCCGATGTCCGGCAACAACGTATCGCACG CCAACAACAAAACCAAACGCCGTTTTTTGCCCAACTTGCAATCACGTCGTTTTTGGGTAGAAAGTGAAAAC CGCTGGGTTCGCCTGCGCGTTTCCAACGCTGCACTGCGTACCATCGACAAAGTAGGCATTGATGTCGTAT TGGCTGATTTGCGTGCTCGCGGCGAAGCTTAA

RNA SEQ ID NO: 159

UUAAGCUUCGCCGCGAGCACGCAAAUCAGCCAAUACGACAUCAAUGCCUACUUUGUCGAUGGUACGCAGUGCAGCGUUGGAAACGCGCAGGCGAACCCAGCGGUUUUCACUUUCUACCCAAAAACGACGUGAUUGCAAGUUGGGCAAAAAACGGCGUUUGGUUUUGUUGUUGGCGUGCGAUACGUUGUUGCCGGACAUCGGGCGUUUGCCGGUCACUUUGCAAACUCGUGCCAU

ANNEX D

>ng_165_porB SEQ ID NO: 178

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_166_porB SEQ ID NO: 179

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATAATCAATTTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_167_porB SEQ ID NO: 180

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGTCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCGCTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAAGGCGGCTTCGGCACCATCCGCGTCGGCAGCCTGAACAGCCCCCTGAAAAACACCGGTGCCAACGTCAATGCTTGGGAATCCGGCAAATATACCGGCGAGCTTCTGGAAATCAGCAAAATGGCCGGACGGGAACACCGCTACCTGTCCGCACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGGTAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAGCGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACGATGATCAAACTTATAGTATGCCCAGTCTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTAGGCGGTTACGACAATAATGCCCTGTACGTTTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAACGAGGGTTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGCGGCATACCGTTTCGGCAATGTAACGCCCCGCGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTATGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTGCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_168_porB SEQ ID NO: 181

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_169_porB SEQ ID NO: 182

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCEGAAAGCCAGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCTGAATACCTATGGTCAAACTTAIAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAACGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_170_porB SEQ ID NO: 183

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_171_porB SEQ ID NO: 184

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_172_porB SEQ ID NO: 185

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_173_porB SEQ ID NO: 186

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAACGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_174_porB SEQ ID NO: 187

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_175_porB SEQ ID NO: 188

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGTCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCGCTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAAGGCGGCTTCGGCACCATCCGCGTCGGCAGCCTGAACAGCCCCCTGAAAAACACCGGTGCCAACGTCAATGCTTGGGAATCCGGCAAATATACCGGCGAGCTTCTGGAAATCAGCAAAATGGCCGGACGGGAACACCGCTACCTGTCCGCACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGGTAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAGCGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACGATGATCAAACTTATAGTATGCCCAGTCTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTAGGCGGTTACGACAATAATGCCCTGTACGTTTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAACGAGGGTTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGCGGCATACCGTTTCGGCAATGTAACGCCCCGCGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTATGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_176_porB SEQ ID NO: 189

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGGCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_177_porB SEQ ID NO: 190

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_178_porB SEQ ID NO: 191

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_179_porB SEQ ID NO: 192

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGAAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGTCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGTGCCTCCGTCGCCGGCACTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAAGGCGGCTTCGGCACCATCCGCGTCGGCAGCCTGAACAGCCCCCTGAAAAACACCGGTGCCAACGTCAATGCTTGGGAATCCGGCAAATATACCGGCGAGTTTCTGGAAATCAGCAAAATGGCCAGACGGGAACACCGCTACCTGTCCGCACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGATAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACGGCGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACGCTGGTCAATATTATAGTATCCCCAGCCTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAACATGGAGTGCTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGCGGCATACCGTTTCGGCAACTTAACGCCCCGCGTTTCTTACGCCCACGGCTTCAAAGGCTCTGTTCATAGTGCAGACTACGACAATACTTATGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGAAAAAGTCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_180_porB SEQ ID NO: 193

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_181_porB SEQ ID NO: 194

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGACGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCGCTAACAGCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCGGCAGCAAAGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAGTGTGCTGAAAATCAGCGGAATGGCCGAACGGGAACACCGCTACCTGTCCGCACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCAAAACAGCGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACGATGGICAAACTIATAGTATGCCCAGTCTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTAGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAACATGGAGTGCTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTGGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_182_porB SEQ ID NO: 195

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGAAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAATCTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_183_porB SEQ ID NO: 196

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_184_porB SEQ ID NO: 197

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_185_porB SEQ ID NO: 198

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTACTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_186_porB SEQ ID NO: 199

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_187_porB SEQ ID NO: 200

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGTCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAAGGCGGCTTCGGCACCATCCGCGTCGGCAGCCTGAACAGCCCCCIGAAAAACACCGGTGCCAACGTCAATGCTTGGGAAICCGGCAAATATACCGGCGAGCTTCTGGAAAICAGCAAAATGGCCGGACGGGAACACCGCTACCTGTCCGCACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGGTAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGCGGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_188_porB SEQ ID NO: 201

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATTCACCCTGTACGGCGGCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_189_porB SEQ ID NO: 202

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_190_porB SEQ ID NO: 203

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCACCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATACAAAAGGCAAGGTAAGTAAAGTGGAAACCGGCAGCGAAATCGCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGTGCCTCCGTCGCCGGCACTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAGGGCGGCTTCGGTACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGGACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCGATGTGCTGGAAATCAGCGGAATGGCCAAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGATAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACGGCGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACGATAATCAATTTTAATAGTGTCCCCAGCCTGTCTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAACATGGCGTGCTAATTCGCACAACTCTCAAACCGAAGTTGCTGCTACCGCGGCATACCGTTTCGGCAACTTAACGCCCCGCGTTTCTTACGCCCACGGCTTCAAAGGCTCTGTTCATAGTGCAGACTACGACAATACTTATGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_191_porB SEQ ID NO: 204

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGGCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAACTGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_192_porB SEQ ID NO: 205

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_193_porB SEQ ID NO: 206

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCACCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATACAAAAGGCAAGGTAAGTAAAGTGGAAACCGGCAGCGAAATCGCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGTGCCTCCGTCGCCCGGCACTAACACCGGCTCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAGGGCGGCTTCGGTACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGGACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCGATGTGCTGGAAATCAGCGGAATGGCCAAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGATAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACGGCGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACAATAATCAATTTTATAGTGTCCCCAGCCTGTCTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAACATGGCGTGCTAATTCGCACAACTCTCAAACCGAAGTTGCTGCTACCGCGGCATACCGTTTCGGCAACTTAACGCCCCGCGTTTCTTACGCCCACGGCTTCAAAGGCTCTGTTCATAGTGCAGACTACGACAATACTTATGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGCAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_194_porB SEQ ID NO: 207

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGAAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGTCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGTGCCTCCGTCGCCGGCACTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAGGGCGGCTTCGGTACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAACGCCAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCAAAATGGCCGAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGATAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACGGCGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACGATAATCAATCTTATAGTATCCCCAGCCTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTAGGCGGTTACGACAATAATGCCCTGTACGTCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGATCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGCGGCATACCGTTTCGGCAACGTAACGCCCCGCGTTTCTTACGCCCACGGCTTCAAAGGCAGTGTTGATAGTGCAAACCACGACAATACTTATGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_195_porB SEQ ID NO: 208

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATAATCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_196_porB SEQ ID NO: 209

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGACAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATAATCAATTTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_197_porB SEQ ID NO: 210

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGACAGCGAAATCTCCGACTTCGGTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACAATAATCAATTTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_198_porB SEQ ID NO: 211

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_199_porB SEQ ID NO: 212

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCACCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATACAAAAGGCAAGGTAAGTAAAGTGGAAACCGGCAGCGAAATCGCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGTGCCTCCGTCGCCGGCACTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAGGGCGGCTTCGGTACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGGACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCGATGTGCTGGAAATCAGCGGAATGGCCAAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGATAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACGGCGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACAATAATCAATTTTATAGTGTCCCCAGCCTGTCTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAACATGGCGTGCTAATTCGCACAACTCTCAAACCGAAGTTGCTGCTACCGCGGCATACCGTTTCGGCAACTTAACGCCCCGCGTTTCTTACGCCCACGGCTTCAAAGGCTCTGTTCATAGTGCAGACTACGACAATACTTATGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGCAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_200_porB SEQ ID NO: 213

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAARTCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCGGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_201_porB SEQ ID NO: 214

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCGGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_202_porB SEQ ID NO: 215

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGTCAAGAAGACCTCGGCAACGGCCTGAAGGCCGTTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACACCGGCTGGGGCAACAAACAATCCTTCGTCGGCTTGAAAGGCGGCTTCGGCACCATCCGCGTCGGCAGCCTGAACAGCCCCCTGAAAAACACCGGTGCCAACGTCAATGCTTGGGAATCCGGCAAATATACCGGCGAGCTTCTGGAAATCAGCAAAATGGCCGGACGGGAACACCGCTACCTGTCCGCACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGGTAATTCAGGCTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAGCGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACTACGATGATCAAACTTATAGTATGCCCAGTCTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTAGGCGGTTACGACAATAATGCCCTGTACGTTTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAACGAGGGTTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGCGGCATACCGTTTCGGCAATGTAACGCCCCGCGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTATGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_203_porB SEQ ID NO: 216

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCGGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_204_porB SEQ ID NO: 217

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGAAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_205_porB SEQ ID NO: 218

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_206_porB SEQ ID NO: 219

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCCAGAAGGCAAAGTAATTGGCGTGGAAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_207_porB SEQ ID NO: 220

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAGTTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_208_porB SEQ ID NO: 221

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_209_porB SEQ ID NO: 222

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_210_porB SEQ ID NO: 223

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCGGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_211_porB SEQ ID NO: 224

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTTACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCAGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_212_porB SEQ ID NO: 225

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGAAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATCGAATACGATGGTCAAACTTATAATATCCCCGGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_213_porB SEQ ID NO: 226

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGAAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGTCAAACTTATAATATCCCCGGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

>ng_214_porB SEQ ID NO: 227

ATGAAAAAATCCCTGATTGCCCTGACTTTGGCAGCCCTTCCTGTTGCGGCAACGGCCGATGTCACCCTGTACGGCGCCATCAAAGCCGGCGTACAAACTTACCGTTCTGTAGAACATCGGGAAGGCAAAGTAATTGGCGTGGGAACCGGCAGCGAAATCTCCGACTTCGGTTCAAAAATCGGCTTCAAAGGCCAAGAAGACCTCGGCAACGGCCTGAAAGCCATTTGGCAGTTGGAACAAGGCGCCTCCGTCGCCGGCACTAACAGCGGCTGGGGCAACAAACAATCCTTCATCGGCTTGAAGGGCGGCTTCGGCACCATCCGCGCCGGTAGCCTGAACAGCCCCCTGAAAAACACCAAGAACAACGTCAATGCTTGGGAATCCGGCAAATTTACCGGCAATGTGCTGGAAATCAGCGGAATGGCCCAACGGGAACACCGCTACCTGTCCGTACGCTACGATTCTCCCGAATTTGCCGGCTTCAGCGGCAGCGTACAATACGCACCTAAAGACAATTCAGGGTCAAACGGCGAATCTTACCACGTTGGCTTGAACTACCGAAACAACGGCTTCTTCGCACAATACGCCGGCTTGTTCCAAAGATACGGCGAAGGCACTAAAAAAATGGAATACGATGGECAAACITAIAATATCCCCGGTTTGTTTGTTGAAAAACTGCAAGTTCACCGTTTGGTCGGCGGTTACGACAATAATGCCCTGTACGCCTCCGTAGCCGCACAACAACAAGATGCCAAATTGTATGGAGCAATGAGCGGTAATTCGCACAACTCTCAAACCGAAGTTGCCGCTACCGTAGCATACCGTTTCGGCAACGTAACGCCCCGTGTTTCTTACGCCCACGGCTTCAAAGGCACTGTTGATGATGCAAACCACGACAATACTTACGACCAAGTGGTTGTCGGTGCGGAATACGACTTCTCCAAACGCACTTCTGCCTTGGTTTCTGCCGGCTGGTTGCAAGAAGGCAAAGGCGCAGACAAAATCGTATCGACTGCCAGCGCCGTCGTTCTGCGCCACAAATTCTAA

ANNEX E Sequences for Exemplary RNA With Low C:T Ratios to Be Used forNormalization 1. NGO0066a: NC_002946.2:c69710-69021

DNA (- strand): SEQ ID NO: 231

GTGCAGGCGGATTTAGCCTACGCCGCCGAACGCATTACCCACGATTATCCGGAA  CCAACCGGTGCAAAAAAAGGCAAAATAAGCACGGTAAGCGATTATTTCAGAAAC  ATCCGTACGCATTCCATCCACCCCAGGGTGTCGGTCGGCTACGACTTCGGCGGCT  GGAGGATAGCGGCAGATTATGCCCGTTACAGAAAGTGGAACAACAGTAAATATT  CCGTCAACACAAAAAAGGTGAACGAAAACAAGGGCGAAAAGATAAACGTGACG  CAATATCTGAAGGCGGAAAATCAGGAAAACGGTACGTTCCACGCCGTTTCTTCTC  TCGGCTTGTCCGCCGTTTACGATTTCAAACTCAACGACAAATTCAAACCCTATAT  CGGCATGCGCGTCGGCTACGGGCACGTCAGACATCAGGTTCGTTCGGTTGAACA  AGAAACCACGACTGTTACCACTTACCTACAGAGTGGTAAGCCAAGTCCTATCGT  ACGAGGTTCGACCCTCAAACTTCCCCATCACGAAAGCCGCAGCAGCCGCCGCTT  GGGCTTCGGCGCGATGGCGGGCGTGGGCATAGACGTCGCGCCCGGTCTGACCTT  GGACGCCGGCTACCGCTACCACTATTGGGGACGCCTGGAAAACACCCGCTTCAA  AACCCACGAAGCCTCGTTGGGCGTGCGCTACCGCTTCTGA

RNA SEQ ID NO: 232

GUGCAGGCGGAUUUAGCCUACGCCGCCGAACGCAUUACCCACGAUUAUCCGGA  ACCAACCGGUGCAAAAAAAGGCAAAAUAAGCACGGUAAGCGAUUAUUUCAGA  AACAUCCGUACGCAUUCCAUCCACCCCAGGGUGUCGGUCGGCUACGACUUCGG  CGGCUGGAGGAUAGCGGCAGAUUAUGCCCGUUACAGAAAGUGGAACAACAGU  AAAUAUUCCGUCAACACAAAAAAGGUGAACGAAAACAAGGGCGAAAAGAUAA  ACGUGACGCAAUAUCUGAAGGCGGAAAAUCAGGAAAACGGUACGUUCCACGC  CGUUUCUUCUCUCGGCUUGUCCGCCGUUUACGAUUUCAAACUCAACGACAAAU  UCAAACCCUAUAUCGGCAUGCGCGUCGGCUACGGGCACGUCAGACAUCAGGUU  CGUUCGGUUGAACAAGAAACCACGACUGUUACCACUUACCUACAGAGUGGUA  AGCCAAGUCCUAUCGUACGAGGUUCGACCCUCAAACUUCCCCAUCACGAAAGC  CGCAGCAGCCGCCGCUUGGGCUUCGGCGCGAUGGCGGGCGUGGGCAUAGACGU  CGCGCCCGGUCUGACCUUGGACGCCGGCUACCGCUACCACUAUUGGGGACGCC  UGGAAAACACCCGCUUCAAAACCCACGAAGCCUCGUUGGGCGUGCGCUACCGC   UUCUGA

cDNA: SEQ ID NO: 233

TCAGAAGCGGTAGCGCACGCCCAACGAGGCTTCGTGGGTTTTGAAGCGGGTGTT  TTCCAGGCGTCCCCAATAGTGGTAGCGGTAGCCGGCGTCCAAGGTCAGACCGGG  CGCGACGTCTATGCCCACGCCCGCCATCGCGCCGAAGCCCAAGCGGCGGCTGCT  GCGGCTTTCGTGATGGGGAAGTTTGAGGGTCGAACCTCGTACGATAGGACTTGG  CTTACCACTCTGTAGGTAAGTGGTAACAGTCGTGGTTTCTTGTTCAACCGAACGA  ACCTGATGTCTGACGTGCCCGTAGCCGACGCGCATGCCGATATAGGGTTTGAATT  TGTCGTTGAGTTTGAAATCGTAAACGGCGGACAAGCCGAGAGAAGAAACGGCGT  GGAACGTACCGTTTTCCTGATTTTCCGCCTTCAGATATTGCGTCACGTTTATCTTT  TCGCCCTTGTTTTCGTTCACCTTTTTTGTGTTGACGGAATATTTACTGTTGTTCCAC  TTTCTGTAACGGGCATAATCTGCCGCTATCCTCCAGCCGCCGAAGTCGTAGCCGA  CCGACACCCTGGGGTGGATGGAATGCGTACGGATGTTTCTGAAATAATCGCTTAC  CGTGCTTATTTTGCCTTTTTTTGCACCGGTTGGTTCCGGATAATCGTGGGTAATGC  GTTCGGCGGCGTAGGCTAAATCCGCCTGCAC

2. NGO0070: NC_002946.2: C75580-74783

DNA (- strand): SEQ ID NO: 234

ATGAATCCAGCCCGCAAAAAACCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT  CTTCTCTTCTCTTCGGCAGCGCAGGCGGCAAGTGAAGGCAATGGCCGCGGCCCG  TATGTGCAGGCGGATTTAGCCTACGCCGCCGAACGCATTACCCACGATTATCCGG  AACCAACCGCTCCAGGCAAAAACAAAATAAGCACGGTAAGCGATTATTTCAGAA  ACATCCGTACGCATTCCATCCACCCCAGGGTGTCGGTCGGCTACGACTTCGGCGG  CTGGAGGATAGCGGCAGATTATGCCCGTTACAGAAAGTGGAACGACAATAAATA  TTCCGTCGACATAAAAGAGTTGGAAAACAAGAATCAGAATAAGAGAGACCTGA  AGACGGAAAATCAGGAAAACGGCAGCTTCCACGCCGTTTCTTCTCTCGGCTTATC  AGCCGTTTACGATTTCAAACTCAACGACAAATTCAAACCCTATATCGGTGCGCGC  GTCGCCTACGGACACGTCAGACACAGCATCGATTCGACTAAAAAAATAACAGGT  ACTCTTACCGCCTACCCTAGTGATGCTGACGCAGCAGTTACGGTTTATCCTGACG  GACATCCGCAAAAAAACACCTATCAAAAAAGCAACAGCAGCCGCCGCTTGGGCT  TCGGCGCGATGGCGGGCGTGGGCATAGACGTCGCGCCCGGCCTGACCTTGGACG  CCGGCTACCGCTACCACAACTGGGGACGCTTGGAAAACACCCGCTTCAAAACCC  ACGAAGCCTCATTGGGCATGCGCTACCGCTTCTGA

RNA: SEQ ID NO: 235

AUGAAUCCAGCCCGCAAAAAACCUUCUCUUCUCUUCUCUUCUCUUCUCUUCUC  UUCUCUUCUCUUCUCUUCGGCAGCGCAGGCGGCAAGUGAAGGCAAUGGCCGCG  GCCCGUAUGUGCAGGCGGAUUUAGCCUACGCCGCCGAACGCAUUACCCACGAU  UAUCCGGAACCAACCGCUCCAGGCAAAAACAAAAUAAGCACGGUAAGCGAUU  AUUUCAGAAACAUCCGUACGCAUUCCAUCCACCCCAGGGUGUCGGUCGGCUAC  GACUUCGGCGGCUGGAGGAUAGCGGCAGAUUAUGCCCGUUACAGAAAGUGGA  ACGACAAUAAAUAUUCCGUCGACAUAAAAGAGUUGGAAAACAAGAAUCAGAA  UAAGAGAGACCUGAAGACGGAAAAUCAGGAAAACGGCAGCUUCCACGCCGUU  UCUUCUCUCGGCUUAUCAGCCGUUUACGAUUUCAAACUCAACGACAAAUUCAA  ACCCUAUAUCGGUGCGCGCGUCGCCUACGGACACGUCAGACACAGCAUCGAUU  CGACUAAAAAAAUAACAGGUACUCUUACCGCCUACCCUAGUGAUGCUGACGCA  GCAGUUACGGUUUAUCCUGACGGACAUCCGCAAAAAAACACCUAUCAAAAAA  GCAACAGCAGCCGCCGCUUGGGCUUCGGCGCGAUGGCGGGCGUGGGCAUAGAC  GUCGCGCCCGGCCUGACCUUGGACGCCGGCUACCGCUACCACAACUGGGGACG  CUUGGAAAACACCCGCUUCAAAACCCACGAAGCCUCAUUGGGCAUGCGCUACC   GCUUCUGA

cDNA: SEQ ID NO: 236

TCAGAAGCGGTAGCGCATGCCCAATGAGGCTTCGTGGGTTTTGAAGCGGGTGTTT  TCCAAGCGTCCCCAGTTGTGGTAGCGGTAGCCGGCGTCCAAGGTCAGGCCGGGC  GCGACGTCTATGCCCACGCCCGCCATCGCGCCGAAGCCCAAGCGGCGGCTGCTG  TTGCTTTTTTGATAGGTGTTTTTTTGCGGATGTCCGTCAGGATAAACCGTAACTGC  TGCGTCAGCATCACTAGGGTAGGCGGTAAGAGTACCTGTTATTTTTTTAGTCGAA  TCGATGCTGTGTCTGACGTGTCCGTAGGCGACGCGCGCACCGATATAGGGTTTGA  ATTTGTCGTTGAGTTTGAAATCGTAAACGGCTGATAAGCCGAGAGAAGAAACGG  CGTGGAAGCTGCCGTTTTCCTGATTTTCCGTCTTCAGGTCTCTCTTATTCTGATTC  TTGTTTTCCAACTCTTTTATGTCGACGGAATATTTATTGTCGTTCCACTTTCTGTA  ACGGGCATAATCTGCCGCTATCCTCCAGCCGCCGAAGTCGTAGCCGACCGACAC  CCTGGGGTGGATGGAATGCGTACGGATGTTTCTGAAATAATCGCTTACCGTGCTT  ATTTTGTTTTTGCCTGGAGCGGTTGGTTCCGGATAATCGTGGGTAATGCGTTCGG  CGGCGTAGGCTAAATCCGCCTGCACATACGGGCCGCGGCCATTGCCTTCACTTGC  CGCCTGCGCTGCCGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG  AGAAGAGAAGGTTTTTTGCGGGCTGGATTCAT

3. Ngo0372: Nc_002946.2:366358-367185

DNA (+ strand): SEQ ID NO: 237

ATGATGTTGAAAAAATTCGTACTCGGCGGCATTGCCGCATTGGTTTTGGCGGCCT  GCGGCGGTTCGGAAGGCGGCAGCGGAGCATCTTCCGCGCCTGCACAATCGGCAA  TTTCCGGTTCTTTAATCGAGCGCATCAACAATAAAGGCACGGTTACCGTCGGCAC  GGAAGGCACTTACGCACCGTTTACCTACCACGACAAAGACGGCAAACTGACCGG  TTACGACGTGGAAGTAACCCGCGCCGTGGCGGAAAAACTGGGCGTAAAAGTCGA  GTTTAAAGAAACGCAATGGGATTCGATGATGGCGGGTTTGAAAGCCGGACGTTT  CGACGTGGTGGCAAACCAAGTCGGCCTGACCAGCCCCGAACGCCAGGCGACATT  TGACAAATCCGAACCTTACAGCTGGAGCGGTGCGGTTTTGGTTGCGCATAACGA  CAGCAACATTAAATCCATAGCCGACATCAAAGGCGTGAAAACCGCGCAATCCCT  GACCAGCAACTACGGCGAAAAAGCCAAAGCCGCAGGTGCGCAACTCGTGCCGG  TGGACGGTTTGGCGCAATCGCTGACCCTGATTGAACAAAAACGCGCCGATGCGA  CGTTGAACGATGAATTGGCGGTTTTGGACTATCTGAAGAAAAACCCGAATGCGG  GGGTGAAAATCGTGTGGTCCGCGCCTGCCGATGAAAAAGTCGGTTCCGGTCTGA  TTGTCAACAAGGGCAATGACGAGGCCGTGGCGAAATTCAGCACGGCAATCAACG  AGCTGAAAGCCGACGGCACGTTGAAAAAACTGGGCGAACAATTCTTCGGAAAAG  ACATCAGTGTTCAATAA

RNA SEQ ID NO: 238

AUGAUGUUGAAAAAAUUCGUACUCGGCGGCAUUGCCGCAUUGGUUUUGGCGG  CCUGCGGCGGUUCGGAAGGCGGCAGCGGAGCAUCUUCCGCGCCUGCACAAUCG  GCAAUUUCCGGUUCUUUAAUCGAGCGCAUCAACAAUAAAGGCACGGUUACCG  UCGGCACGGAAGGCACUUACGCACCGUUUACCUACCACGACAAAGACGGCAAA  CUGACCGGUUACGACGUGGAAGUAACCCGCGCCGUGGCGGAAAAACUGGGCG  UAAAAGUCGAGUUUAAAGAAACGCAAUGGGAUUCGAUGAUGGCGGGUUUGAA  AGCCGGACGUUUCGACGUGGUGGCAAACCAAGUCGGCCUGACCAGCCCCGAAC  GCCAGGCGACAUUUGACAAAUCCGAACCUUACAGCUGGAGCGGUGCGGUUUU  GGUUGCGCAUAACGACAGCAACAUUAAAUCCAUAGCCGACAUCAAAGGCGUG  AAAACCGCGCAAUCCCUGACCAGCAACUACGGCGAAAAAGCCAAAGCCGCAGG  UGCGCAACUCGUGCCGGUGGACGGUUUGGCGCAAUCGCUGACCCUGAUUGAAC  AAAAACGCGCCGAUGCGACGUUGAACGAUGAAUUGGCGGUUUUGGACUAUCU  GAAGAAAAACCCGAAUGCGGGGGUGAAAAUCGUGUGGUCCGCGCCUGCCGAU  GAAAAAGUCGGUUCCGGUCUGAUUGUCAACAAGGGCAAUGACGAGGCCGUGG  CGAAAUUCAGCACGGCAAUCAACGAGCUGAAAGCCGACGGCACGUUGAAAAA  ACUGGGCGAACAAUUCUUCGGAAAAGACAUCAGUGUUCAAUAA

cDNA: SEQ ID NO: 239

TTATTGAACACTGATGTCTTTTCCGAAGAATTGTTCGCCCAGTTTTTTCAACGTGC  CGTCGGCTTTCAGCTCGTTGATTGCCGTGCTGAATTTCGCCACGGCCTCGTCATTG  CCCTTGTTGACAATCAGACCGGAACCGACTTTTTCATCGGCAGGCGCGGACCAC  ACGATTTTCACCCCCGCATTCGGGTTTTTCTTCAGATAGTCCAAAACCGCCAATT  CATCGTTCAACGTCGCATCGGCGCGTTTTTGTTCAATCAGGGTCAGCGATTGCGC  CAAACCGTCCACCGGCACGAGTTGCGCACCTGCGGCTTTGGCTTTTTCGCCGTAG  TTGCTGGTCAGGGATTGCGCGGTTTTCACGCCTTTGATGTCGGCTATGGATTTAA  TGTTGCTGTCGTTATGCGCAACCAAAACCGCACCGCTCCAGCTGTAAGGTTCGGA  TTTGTCAAATGTCGCCTGGCGTTCGGGGCTGGTCAGGCCGACTTGGTTTGCCACC  ACGTCGAAACGTCCGGCTTTCAAACCCGCCATCATCGAATCCCATTGCGTTTCTT  TAAACTCGACTTTTACGCCCAGTTTTTCCGCCACGGCGCGGGTTACTTCCACGTC  GTAACCGGTCAGTTTGCCGTCTTTGTCGTGGTAGGTAAACGGTGCGTAAGTGCCT  TCCGTGCCGACGGTAACCGTGCCTTTATTGTTGATGCGCTCGATTAAAGAACCGG  AAATTGCCGATTGTGCAGGCGCGGAAGATGCTCCGCTGCCGCCTTCCGAACCGC  CGCAGGCCGCCAAAACCAATGCGGCAATGCCGCCGAGTACGAATTTTTTCAACA   TCAT

4. Ngo0374: Nc_002946.2:367901-368656

DNA (+ strand): SEQ ID NO: 240

ATGATTAAAATCCGCAATATCCATAAGACCTTTGGCGAAAACACCATTTTGCGCG  GCATCGATTTGGATGTGGGCAAAGGGCAGGTGGTCGTCATCCTCGGGCCTTCCG  GCTCGGGTAAAACAACATTTCTGCGCTGCCTAAACGCGTTGGAAATGCCCGAAG  ACGGACAAATCGAGTTCGACAACGCGCGGCCGTTACGCATTGATTTTTCCAAAA  AAACAAGCAAACACGATATTTTGGCACTGCGCCGCAAGTCCGGAATGGTATTCC  AACAATACAACCTCTTCCCGCATAAAACCGTGTTGGAAAACGTGATGGAAGGGC  CGGTTGCCGTACAGGGCAAGCCTGCCGCCCAAGCGCGCGAAGAGGCTTTGAAAC  TGCTGGAAAAAGTCGGCTTGGGCGATAAAGTGGATTTGTATCCCTACCAGCTTTC  CGGCGGTCAGCAGCAGCGTGTCGGTATCGCCCGCGCACTGGCGATTCAGCCTGA  ATTGATGCTGTTTGACGAACCCACTTCCGCGCTGGACCCCGAGTTGGTGCAAGAC  GTGTTGGACGCCATGAAGGAATTGGCGCGGGAAGGTTGGACGATGGTCGTCGTT  ACCCACGAAATCAAGTTCACGCTGGAAGTTGCCACGAACGTCGTCGTGATGGAC  GGCGGCGTTATCGTAGAGCAGGGCAGCCCGAAAGAGTTGTTCGACCACCTCAAA  CACGAACGGACGCGGAGATTTTTAAGCCAAATCCAATCTGCCAAGATTTGA

RNA SEQ ID NO: 241

AUGAUUAAAAUCCGCAAUAUCCAUAAGACCUUUGGCGAAAACACCAUUUUGC  GCGGCAUCGAUUUGGAUGUGGGCAAAGGGCAGGUGGUCGUCAUCCUCGGGCC  UUCCGGCUCGGGUAAAACAACAUUUCUGCGCUGCCUAAACGCGUUGGAAAUG  CCCGAAGACGGACAAAUCGAGUUCGACAACGCGCGGCCGUUACGCAUUGAUUU  UUCCAAAAAAACAAGCAAACACGAUAUUUUGGCACUGCGCCGCAAGUCCGGA  AUGGUAUUCCAACAAUACAACCUCUUCCCGCAUAAAACCGUGUUGGAAAACG  UGAUGGAAGGGCCGGUUGCCGUACAGGGCAAGCCUGCCGCCCAAGCGCGCGAA  GAGGCUUUGAAACUGCUGGAAAAAGUCGGCUUGGGCGAUAAAGUGGAUUUGU  AUCCCUACCAGCUUUCCGGCGGUCAGCAGCAGCGUGUCGGUAUCGCCCGCGCA  CUGGCGAUUCAGCCUGAAUUGAUGCUGUUUGACGAACCCACUUCCGCGCUGGA  CCCCGAGUUGGUGCAAGACGUGUUGGACGCCAUGAAGGAAUUGGCGCGGGAA  GGUUGGACGAUGGUCGUCGUUACCCACGAAAUCAAGUUCACGCUGGAAGUUG  CCACGAACGUCGUCGUGAUGGACGGCGGCGUUAUCGUAGAGCAGGGCAGCCCG  AAAGAGUUGUUCGACCACCUCAAACACGAACGGACGCGGAGAUUUUUAAGCC  AAAUCCAAUCUGCCAAGAUUUGA

cDNA: SEQ ID NO: 242

TCAAATCTTGGCAGATTGGATTTGGCTTAAAAATCTCCGCGTCCGTTCGTGTTTG  AGGTGGTCGAACAACTCTTTCGGGCTGCCCTGCTCTACGATAACGCCGCCGTCCA  TCACGACGACGTTCGTGGCAACTTCCAGCGTGAACTTGATTTCGTGGGTAACGAC  GACCATCGTCCAACCTTCCCGCGCCAATTCCTTCATGGCGTCCAACACGTCTTGC  ACCAACTCGGGGTCCAGCGCGGAAGTGGGTTCGTCAAACAGCATCAATTCAGGC  TGAATCGCCAGTGCGCGGGCGATACCGACACGCTGCTGCTGACCGCCGGAAAGC  TGGTAGGGATACAAATCCACTTTATCGCCCAAGCCGACTTTTTCCAGCAGTTTCA  AAGCCTCTTCGCGCGCTTGGGCGGCAGGCTTGCCCTGTACGGCAACCGGCCCTTC  CATCACGTTTTCCAACACGGTTTTATGCGGGAAGAGGTTGTATTGTTGGAATACC  ATTCCGGACTTGCGGCGCAGTGCCAAAATATCGTGTTTGCTTGTTTTTTTGGAAA  AATCAATGCGTAACGGCCGCGCGTTGTCGAACTCGATTTGTCCGTCTTCGGGCAT  TTCCAACGCGTTTAGGCAGCGCAGAAATGTTGTTTTACCCGAGCCGGAAGGCCC  GAGGATGACGACCACCTGCCCTTTGCCCACATCCAAATCGATGCCGCGCAAAAT  GGTGTTTTCGCCAAAGGTCTTATGGATATTGCGGATTTTAATCAT

5. NGO0399: NC_002946.2: C392291-391452

DNA (- strand): SEQ ID NO: 243

GTGAAACGCATTTTTCTGTTTTTGGCTACCAATATCGCTGTTTTGGTCGTAATCAA  CATTGTTTTGGCGGTTCTGGGCATCAACAGCCGGGGCGGCGCGGGCAGCCTGTTG  GCGTATTCCGCCGTCGTCGGCTTCACTGGTTCGATTATTTCGCTGCTGATGTCCAA  ATTTATCGCCAAACAATCGGTCGGTGCGGAAGTCATCGACACGCCGCGCACCGA  AGAAGAAGCCTGGCTTCTGAACACTGTCGAAGCCCAAGCGCGGCAATGGAATCT  GAAAACGCCAGAAGTCGCCATCTACCACTCCCCCGAACCCAATGCCTTTGCCAC  GGGCGCATCGAGAAACAGCTCCCTGATCGCCGTCAGCACCGGTTTGCTCGACCA  TATGACGCGCGACGAAGTGGAAGCCGTGTTGGCGCACGAAATGGCGCACGTCGG  CAACGGCGACATGGTTACGCTGACGCTGATTCAAGGCGTGGTCAATACCTTTGTC  GTGTTCCTGTCGCGCATTATTGCCAACCTGATTGCCCGAAACAACGACGGCAGCC  AGTCCCAGGGAACTTATTTCCTAGTCAGCATGGTATTCCAAATCCTGTTCGGCTT  CCTTGCCAGCCTGATTGTCATGTGGTTCAGCCGCCAACGCGAATACCGCGCCGAC  GCGGGCGCGGCAAAACTGGTCGGCGCACCGAAAATGATTTCCGCCCTGCAAAGG  CTTAAAGGCAACCCGGTCGATTTGCCCGAAGAAATGAACGCAATGGGCATCGCC  GGAGATACGCGCGACTCCCTGCTCAGCACCCACCCTTCGCTGGACAACCGAATC  GCCCGCCTCAAATCGCTTTAA

RNA SEQ ID NO: 244

GUGAAACGCAUUUUUCUGUUUUUGGCUACCAAUAUCGCUGUUUUGGUCGUAA  UCAACAUUGUUUUGGCGGUUCUGGGCAUCAACAGCCGGGGCGGCGCGGGCAG  CCUGUUGGCGUAUUCCGCCGUCGUCGGCUUCACUGGUUCGAUUAUUUCGCUGC  UGAUGUCCAAAUUUAUCGCCAAACAAUCGGUCGGUGCGGAAGUCAUCGACAC  GCCGCGCACCGAAGAAGAAGCCUGGCUUCUGAACACUGUCGAAGCCCAAGCGC  GGCAAUGGAAUCUGAAAACGCCAGAAGUCGCCAUCUACCACUCCCCCGAACCC  AAUGCCUUUGCCACGGGCGCAUCGAGAAACAGCUCCCUGAUCGCCGUCAGCAC  CGGUUUGCUCGACCAUAUGACGCGCGACGAAGUGGAAGCCGUGUUGGCGCAC  GAAAUGGCGCACGUCGGCAACGGCGACAUGGUUACGCUGACGCUGAUUCAAG  GCGUGGUCAAUACCUUUGUCGUGUUCCUGUCGCGCAUUAUUGCCAACCUGAU  UGCCCGAAACAACGACGGCAGCCAGUCCCAGGGAACUUAUUUCCUAGUCAGCA  UGGUAUUCCAAAUCCUGUUCGGCUUCCUUGCCAGCCUGAUUGUCAUGUGGUU  CAGCCGCCAACGCGAAUACCGCGCCGACGCGGGCGCGGCAAAACUGGUCGGCG  CACCGAAAAUGAUUUCCGCCCUGCAAAGGCUUAAAGGCAACCCGGUCGAUUUG  CCCGAAGAAAUGAACGCAAUGGGCAUCGCCGGAGAUACGCGCGACUCCCUGCU  CAGCACCCACCCUUCGCUGGACAACCGAAUCGCCCGCCUCAAAUCGCUUUAA

cDNA: SEQ ID NO: 245

TTAAAGCGATTTGAGGCGGGCGATTCGGTTGTCCAGCGAAGGGTGGGTGCTGAG  CAGGGAGTCGCGCGTATCTCCGGCGATGCCCATTGCGTTCATTTCTTCGGGCAAA  TCGACCGGGTTGCCTTTAAGCCTTTGCAGGGCGGAAATCATTTTCGGTGCGCCGA  CCAGTTTTGCCGCGCCCGCGTCGGCGCGGTATTCGCGTTGGCGGCTGAACCACAT  GACAATCAGGCTGGCAAGGAAGCCGAACAGGATTTGGAATACCATGCTGACTAG  GAAATAAGTTCCCTGGGACTGGCTGCCGTCGTTGTTTCGGGCAATCAGGTTGGCA  ATAATGCGCGACAGGAACACGACAAAGGTATTGACCACGCCTTGAATCAGCGTC  AGCGTAACCATGTCGCCGTTGCCGACGTGCGCCATTTCGTGCGCCAACACGGCTT  CCACTTCGTCGCGCGTCATATGGTCGAGCAAACCGGTGCTGACGGCGATCAGGG  AGCTGTTTCTCGATGCGCCCGTGGCAAAGGCATTGGGTTCGGGGGAGTGGTAGA  TGGCGACTTCTGGCGTTTTCAGATTCCATTGCCGCGCTTGGGCTTCGACAGTGTTC  AGAAGCCAGGCTTCTTCTTCGGTGCGCGGCGTGTCGATGACTTCCGCACCGACCG  ATTGTTTGGCGATAAATTTGGACATCAGCAGCGAAATAATCGAACCAGTGAAGC  CGACGACGGCGGAATACGCCAACAGGCTGCCCGCGCCGCCCCGGCTGTTGATGC  CCAGAACCGCCAAAACAATGTTGATTACGACCAAAACAGCGATATTGGTAGCCA  AAAACAGAAAAATGCGTTTCAC

6. Ngo0453: Nc_002946.2:447935-448546

DNA (+ strand): SEQ ID NO: 246

ATGAAGAATAATGATTGCTTGCGCCTGAAAAATCCCCAGTCCGGTATGGCGTTG  ATAGAAGTCTTGGTCGCTATGCTCGTTCTGACCATCGGTATTTTGGCATTGCTGTC  CGTACAGTTGCGGACAGTCGCTTCCGTCAGGGAGGCGGAAACGCAAACCATCGT  CAGCCAAATCACGCAAAACCTGATGGAAGGAATGTTGATGAATCCGACCATTGA  TTTGGACAGCAACAAGAAAAACTATAGTCTTTACATGGGAAAACAGACACTATC  AGCTGTGGATGGTGAGTTTATGCTTGATGCCGAGAAAAGTAAGGCGCAGTTGGC  AGAGGAACAATTGAAGAGATTTAGTCATGAGCTGAAAAATGCCTTGCCGGATGC  GGTAGCTATTCATTACGCCGTCTGCAAGGATTCGTCGGGTGACGCGCCGACATTG  TCCGACAGCGGTGCTTTTTCTTCAAATTGCGACAATAAGGCAAACGGGGATACTT  TGATTAAAGTATTGTGGGTAAATGATTCGGCAGGGGATTCGGATATTTCCCGTAC  GAATCTTGAAGTGAGCGGCGACAATATCGTATATACCTATCAGGCAAGGGTCGG   AGGTCGTGAATGA

RNA SEQ ID NO: 247

AUGAAGAAUAAUGAUUGCUUGCGCCUGAAAAAUCCCCAGUCCGGUAUGGCGU  UGAUAGAAGUCUUGGUCGCUAUGCUCGUUCUGACCAUCGGUAUUUUGGCAUU  GCUGUCCGUACAGUUGCGGACAGUCGCUUCCGUCAGGGAGGCGGAAACGCAA  ACCAUCGUCAGCCAAAUCACGCAAAACCUGAUGGAAGGAAUGUUGAUGAAUC  CGACCAUUGAUUUGGACAGCAACAAGAAAAACUAUAGUCUUUACAUGGGAAA  ACAGACACUAUCAGCUGUGGAUGGUGAGUUUAUGCUUGAUGCCGAGAAAAGU  AAGGCGCAGUUGGCAGAGGAACAAUUGAAGAGAUUUAGUCAUGAGCUGAAAA  AUGCCUUGCCGGAUGCGGUAGCUAUUCAUUACGCCGUCUGCAAGGAUUCGUC  GGGUGACGCGCCGACAUUGUCCGACAGCGGUGCUUUUUCUUCAAAUUGCGAC  AAUAAGGCAAACGGGGAUACUUUGAUUAAAGUAUUGUGGGUAAAUGAUUCGG  CAGGGGAUUCGGAUAUUUCCCGUACGAAUCUUGAAGUGAGCGGCGACAAUAU  CGUAUAUACCUAUCAGGCAAGGGUCGGAGGUCGUGAAUGA

cDNA: SEQ ID NO: 248

TCATTCACGACCTCCGACCCTTGCCTGATAGGTATATACGATATTGTCGCCGCTC  ACTTCAAGATTCGTACGGGAAATATCCGAATCCCCTGCCGAATCATTTACCCACA  ATACTTTAATCAAAGTATCCCCGTTTGCCTTATTGTCGCAATTTGAAGAAAAAGC  ACCGCTGTCGGACAATGTCGGCGCGTCACCCGACGAATCCTTGCAGACGGCGTA  ATGAATAGCTACCGCATCCGGCAAGGCATTTTTCAGCTCATGACTAAATCTCTTC  AATTGTTCCTCTGCCAACTGCGCCTTACTTTTCTCGGCATCAAGCATAAACTCAC  CATCCACAGCTGATAGTGTCTGTTTTCCCATGTAAAGACTATAGTTTTTCTTGTTG  CTGTCCAAATCAATGGTCGGATTCATCAACATTCCTTCCATCAGGTTTTGCGTGA  TTTGGCTGACGATGGTTTGCGTTTCCGCCTCCCTGACGGAAGCGACTGTCCGCAA  CTGTACGGACAGCAATGCCAAAATACCGATGGTCAGAACGAGCATAGCGACCAA  GACTTCTATCAACGCCATACCGGACTGGGGATTTTTCAGGCGCAAGCAATCATTA   TTCTTCAT

7. Ng00571: Nc_002946.2:553869-555665

DNA (+ strand): SEQ ID NO: 249

ATGCGCTACAAACCCCTTCTGCTTGCCCTGATGCTCGTTTTTTCCACGCCCGCCGT  TGCCGCCCACGACGCGGCACACAACCGTTCCGCCGAAGTGAAAAAACAGGCGA  AGAACAAAAAAGAACAGCCCGAAGCGGCGGAAGGCAAAAAAGAAAAAGGCAA  AAATGCCGCAGTGAAAGATAAAAAAACAGGCGGCAAAGAGGCGGCAAAAGAGT  TCAAAAAAACCGCCAAAAACCGCAAAGAAGCAGAGAAGGAGGCGACATCCAGG  CAGTCTGCGCGCAAAGGACGCGAAGGGGATAAGGAATCGAAGGCGGAACACAA  AAAGGCACATGGCAAGCCCGTGTCCGGATCCAAAGAAAAAAACGCAAAAACAC  AGCCTGAAAACAAACAAGGCAAAAAAGGGGCAAAAGGACAGGGCAATCCGCGC  AAGGGCGGCAAGGCGGAAAAAGACACTGTTTCTGCAAATAAAAAAGCCCGTTCC  GACAAGAACGGCAAAGCAGTGAAACAGGACAAAAAACACACGGAAGAGAAAA  ATGCCAAAACCGATTCCGACGAATTGAAAGCCGCCGTTGCCGCTGCCACCAATG  ATGTCGAAAACAAAAAAGCCCTGCTCAAACAAAGCGAAGGAATGCTGCTTCATG  TCAGCAATTCCCTCAAACAGCTTCAGGAAGAGCGTATCCGCCAAGAACGTATCC  GCCAAGAGCGTATCCGTCAGGCGCGCGGCAACCTTGCTTCCGTCAACCGCAAAC  AGCGCGAGGCTTGGGACAAATTCCAAAAACTCAATACCGAGCTGAACCGTTTGA  AAACGGAAGTCGCCGCTACGAAAGCGCAGATTTCCCGTTTCGTATCGGGGAACT  ATAAAAACAGCCGGCCGAATGCGGTTGCCCTGTTCCTGAAAAACGCCGAACCGG  GTCAGAAAAACCGCTTTTTGCGTTATACGCGTTATGTAAACGCCTCCAATCGGGA  AGTTGTCAAGGATTTGGAAAAACAGCAGAAGGCTTTGGCGGTACAAGAGCAGAA  AATCAACAATGAGCTTGCCCGTTTGAAGAAAATTCAGGCAAACGTGCAATCCCT  GCTGAAAAAACAGGGTGTAACCGATGCGGCGGAACAGACGGAAAGCCGCAGAC  AGAATGCCAAAATCTCCAAAGATGCCCGAAAACTGCTGGAACAGAAAGGGAAC  GAGCAGCAGCTGAACAAGCTCTTGAGCAATTTGGAGAAAAAAAAAGCCGAACA  CCGCATTCAGGATGCGGAAGCAAAAAGAAAATTGGCTGAAGCCAAACTGGCGG  CAGCCGAAAAAGCCAGAAAAGAAGCGGCGCAGCAGAAGGCTGAAGCGCGACGT  GCGGAAATGTCCAACCTGACCGCCGAAGACAGGAACATCCAAGCGCCTTCGGTT  ATGGGTATCGGCAGTGCCGACGGTTTCAGCCGCATGCAGGGACGTTTGAAAAAA  CCGGTTGACGGTGTGCCGACCGGGCTTTTCGGGCAGAACCGGAGCGGCGGCGAT  GTTTGGAAAGGCGTGTTCTATTCCACTGCGCCTGCAACGGTTGAAAGCATTGCGC  CGGGAACGGTAAGCTATGCGGACGAGTTGGACGGCTACGGCAAAGTGGTCGTGA  TCGATCACGGCGAGAACTACATCAGCATCTATGCCGGTTTGAGCGAAATTTCCGC  CGGCAAGGGTTATACGGTCGCGGCAGGAAGCAAAATCGGCACGAGCGGGTCGC  TGCCGGACGGGGAAGAGGGGCTTTACCTGCAAATACGTTATCGAGGTCAGGTGT  TGAACCCTTCGGGCTGGATACGTTGA

RNA: SEQ ID NO: 250

AUGCGCUACAAACCCCUUCUGCUUGCCCUGAUGCUCGUUUUUUCCACGCCCGC  CGUUGCCGCCCACGACGCGGCACACAACCGUUCCGCCGAAGUGAAAAAACAGG  CGAAGAACAAAAAAGAACAGCCCGAAGCGGCGGAAGGCAAAAAAGAAAAAGG  CAAAAAUGCCGCAGUGAAAGAUAAAAAAACAGGCGGCAAAGAGGCGGCAAAA  GAGUUCAAAAAAACCGCCAAAAACCGCAAAGAAGCAGAGAAGGAGGCGACAU  CCAGGCAGUCUGCGCGCAAAGGACGCGAAGGGGAUAAGGAAUCGAAGGCGGA  ACACAAAAAGGCACAUGGCAAGCCCGUGUCCGGAUCCAAAGAAAAAAACGCA  AAAACACAGCCUGAAAACAAACAAGGCAAAAAAGGGGCAAAAGGACAGGGCA  AUCCGCGCAAGGGCGGCAAGGCGGAAAAAGACACUGUUUCUGCAAAUAAAAA  AGCCCGUUCCGACAAGAACGGCAAAGCAGUGAAACAGGACAAAAAACACACG  GAAGAGAAAAAUGCCAAAACCGAUUCCGACGAAUUGAAAGCCGCCGUUGCCG  CUGCCACCAAUGAUGUCGAAAACAAAAAAGCCCUGCUCAAACAAAGCGAAGG  AAUGCUGCUUCAUGUCAGCAAUUCCCUCAAACAGCUUCAGGAAGAGCGUAUCC  GCCAAGAACGUAUCCGCCAAGAGCGUAUCCGUCAGGCGCGCGGCAACCUUGCU  UCCGUCAACCGCAAACAGCGCGAGGCUUGGGACAAAUUCCAAAAACUCAAUAC  CGAGCUGAACCGUUUGAAAACGGAAGUCGCCGCUACGAAAGCGCAGAUUUCCC  GUUUCGUAUCGGGGAACUAUAAAAACAGCCGGCCGAAUGCGGUUGCCCUGUU  CCUGAAAAACGCCGAACCGGGUCAGAAAAACCGCUUUUUGCGUUAUACGCGU  UAUGUAAACGCCUCCAAUCGGGAAGUUGUCAAGGAUUUGGAAAAACAGCAGA  AGGCUUUGGCGGUACAAGAGCAGAAAAUCAACAAUGAGCUUGCCCGUUUGAA  GAAAAUUCAGGCAAACGUGCAAUCCCUGCUGAAAAAACAGGGUGUAACCGAU  GCGGCGGAACAGACGGAAAGCCGCAGACAGAAUGCCAAAAUCUCCAAAGAUG  CCCGAAAACUGCUGGAACAGAAAGGGAACGAGCAGCAGCUGAACAAGCUCUU  GAGCAAUUUGGAGAAAAAAAAAGCCGAACACCGCAUUCAGGAUGCGGAAGCA  AAAAGAAAAUUGGCUGAAGCCAAACUGGCGGCAGCCGAAAAAGCCAGAAAAG  AAGCGGCGCAGCAGAAGGCUGAAGCGCGACGUGCGGAAAUGUCCAACCUGACC  GCCGAAGACAGGAACAUCCAAGCGCCUUCGGUUAUGGGUAUCGGCAGUGCCG  ACGGUUUCAGCCGCAUGCAGGGACGUUUGAAAAAACCGGUUGACGGUGUGCC  GACCGGGCUUUUCGGGCAGAACCGGAGCGGCGGCGAUGUUUGGAAAGGCGUG  UUCUAUUCCACUGCGCCUGCAACGGUUGAAAGCAUUGCGCCGGGAACGGUAA  GCUAUGCGGACGAGUUGGACGGCUACGGCAAAGUGGUCGUGAUCGAUCACGG  CGAGAACUACAUCAGCAUCUAUGCCGGUUUGAGCGAAAUUUCCGCCGGCAAG  GGUUAUACGGUCGCGGCAGGAAGCAAAAUCGGCACGAGCGGGUCGCUGCCGG  ACGGGGAAGAGGGGCUUUACCUGCAAAUACGUUAUCGAGGUCAGGUGUUGAA  CCCUUCGGGCUGGAUACGUUGA   

cDNA: SEQ ID NO: 251

TCAACGTATCCAGCCCGAAGGGTTCAACACCTGACCTCGATAACGTATTTGCAG  GTAAAGCCCCTCTTCCCCGTCCGGCAGCGACCCGCTCGTGCCGATTTTGCTTCCT  GCCGCGACCGTATAACCCTTGCCGGCGGAAATTTCGCTCAAACCGGCATAGATG  CTGATGTAGTTCTCGCCGTGATCGATCACGACCACTTTGCCGTAGCCGTCCAACT  CGTCCGCATAGCTTACCGTTCCCGGCGCAATGCTTTCAACCGTTGCAGGCGCAGT  GGAATAGAACACGCCTTTCCAAACATCGCCGCCGCTCCGGTTCTGCCCGAAAAG  CCCGGTCGGCACACCGTCAACCGGTTTTTTCAAACGTCCCTGCATGCGGCTGAAA  CCGTCGGCACTGCCGATACCCATAACCGAAGGCGCTTGGATGTTCCTGTCTTCGG  CGGTCAGGTTGGACATTTCCGCACGTCGCGCTTCAGCCTTCTGCTGCGCCGCTTC  TTTTCTGGCTTTTTCGGCTGCCGCCAGTTTGGCTTCAGCCAATTTTCTTTTTGCTTC  CGCATCCTGAATGCGGTGTTCGGCTTTTTTTTTCTCCAAATTGCTCAAGAGCTTGT  TCAGCTGCTGCTCGTTCCCTTTCTGTTCCAGCAGTTTTCGGGCATCTTTGGAGATT  TTGGCATTCTGTCTGCGGCTTTCCGTCTGTTCCGCCGCATCGGTTACACCCTGTTT  TTTCAGCAGGGATTGCACGTTTGCCTGAATTTTCTTCAAACGGGCAAGCTCATTG  TTGATTTTCTGCTCTTGTACCGCCAAAGCCTTCTGCTGTTTTTCCAAATCCTTGAC  AACTTCCCGATTGGAGGCGTTTACATAACGCGTATAACGCAAAAAGCGGTTTTTC  TGACCCGGTTCGGCGTTTTTCAGGAACAGGGCAACCGCATTCGGCCGGCTGTTTT  TATAGTTCCCCGATACGAAACGGGAAATCTGCGCTTTCGTAGCGGCGACTTCCGT  TTTCAAACGGTTCAGCTCGGTATTGAGTTTTTGGAATTTGTCCCAAGCCTCGCGCT  GTTTGCGGTTGACGGAAGCAAGGTTGCCGCGCGCCTGACGGATACGCTCTTGGC  GGATACGTTCTTGGCGGATACGCTCTTCCTGAAGCTGTTTGAGGGAATTGCTGAC  ATGAAGCAGCATTCCTTCGCTTTGTTTGAGCAGGGCTTTTTTGTTTTCGACATCAT  TGGTGGCAGCGGCAACGGCGGCTTTCAATTCGTCGGAATCGGTTTTGGCATTTTT  CTCTTCCGTGTGTTTTTTGTCCTGTTTCACTGCTTTGCCGTTCTTGTCGGAACGGG  CTTTTTTATTTGCAGAAACAGTGTCTTTTTCCGCCTTGCCGCCCTTGCGCGGATTG  CCCTGTCCTTTTGCCCCTTTTTTGCCTTGTTTGTTTTCAGGCTGTGTTTTTGCGTTT  TTTTCTTTGGATCCGGACACGGGCTTGCCATGTGCCTTTTTGTGTTCCGCCTTCGA  TTCCTTATCCCCTTCGCGTCCTTTGCGCGCAGACTGCCTGGATGTCGCCTCCTTCT  CTGCTTCTTTGCGGTTTTTGGCGGTTTTTTTGAACTCTTTTGCCGCCTCTTTGCCGC  CTGTTTTTTTATCTTTCACTGCGGCATTTTTGCCTTTTTCTTTTTTGCCTTCCGCCG  CTTCGGGCTGTTCTTTTTTGTTCTTCGCCTGTTTTTTCACTTCGGCGGAACGGTTGT  GTGCCGCGTCGTGGGCGGCAACGGCGGGCGTGGAAAAAACGAGCATCAGGGCA  AGCAGAAGGGGTTTGTAGCGCAT

8. NGO0632: NC_002946.2:c622370-622050

DNA (- strand): SEQ ID NO: 252

ATGATTACCCTTACCGAGAATGCCGCAAAACACATCAATGACTATCTCGCCAAA  CGCGGCAAAGGCTTGGGCGTACGCTTGGGTGTAAAAACCAGCGGCTGCTCGGGG  ATGGCGTACAACCTTGAATTTGTCGATGAAGCCAACGGCGACGACCTGATTTTCG  AAGGACACGGCGCGCGCATTTATATCGACCCGAAAAGCTTGGTTTATCTGGACG  GCACACAAGTCGATTACACCAAAGAAGATTTGCAGGAAGGTTTCAAATTTGAAA  ACCCCAATGTCAAAGACTCCTGCGGCTGCGGCGAGAGCTTCCACGTTTAA

RNA: SEQ ID NO: 253

AUGAUUACCCUUACCGAGAAUGCCGCAAAACACAUCAAUGACUAUCUCGCCAA  ACGCGGCAAAGGCUUGGGCGUACGCUUGGGUGUAAAAACCAGCGGCUGCUCG  GGGAUGGCGUACAACCUUGAAUUUGUCGAUGAAGCCAACGGCGACGACCUGA  UUUUCGAAGGACACGGCGCGCGCAUUUAUAUCGACCCGAAAAGCUUGGUUUA  UCUGGACGGCACACAAGUCGAUUACACCAAAGAAGAUUUGCAGGAAGGUUUC  AAAUUUGAAAACCCCAAUGUCAAAGACUCCUGCGGCUGCGGCGAGAGCUUCCA   CGUUUAA

cDNA: SEQ ID NO: 254

TTAAACGTGGAAGCTCTCGCCGCAGCCGCAGGAGTCTTTGACATTGGGGTTTTCA  AATTTGAAACCTTCCTGCAAATCTTCTTTGGTGTAATCGACTTGTGTGCCGTCCAG  ATAAACCAAGCTTTTCGGGTCGATATAAATGCGCGCGCCGTGTCCTTCGAAAATC  AGGTCGTCGCCGTTGGCTTCATCGACAAATTCAAGGTTGTACGCCATCCCCGAGC  AGCCGCTGGTTTTTACACCCAAGCGTACGCCCAAGCCTTTGCCGCGTTTGGCGAG  ATAGTCATTGATGTGTTTTGCGGCATTCTCGGTAAGGGTAATCAT

9. NGO0633: NC_002946.2:c622843-622457

DNA (- strand): SEQ ID NO: 255

ATGGCATACAGCGATAAAGTAATCGACCACTACGAAAATCCCCGCAACGTCGGC  ACTTTCGACAAAAACGACGAGTCCGTCGGCACCGGCATGGTCGGCGCGCCCGCC  TGCGGCGACGTGATGCGCCTGCAAATCAAAGTGAACGATGAAGGCATCATCGAA  GATGCGAAATTCAAAACTTACGGCTGCGGTTCCGCCATCGCTTCGTCCAGCCTGA  TTACCGAGTGGGTCAAAGGCAAAAGTCTGGATGACGCGCTGGCAATCAAAAACA  GCGAAATCGCCGAAGAACTGGAATTGCCGCCGGTAAAAATCCACTGCTCCATCT  TGGCTGAAGATGCGGTAAAAGCGGCCGTTGCCGACTACCGCAAACGTCAGGAAA   ACAGATAA

RNA SEQ ID NO: 256

AUGGCAUACAGCGAUAAAGUAAUCGACCACUACGAAAAUCCCCGCAACGUCGG  CACUUUCGACAAAAACGACGAGUCCGUCGGCACCGGCAUGGUCGGCGCGCCCG  CCUGCGGCGACGUGAUGCGCCUGCAAAUCAAAGUGAACGAUGAAGGCAUCAU  CGAAGAUGCGAAAUUCAAAACUUACGGCUGCGGUUCCGCCAUCGCUUCGUCCA  GCCUGAUUACCGAGUGGGUCAAAGGCAAAAGUCUGGAUGACGCGCUGGCAAU  CAAAAACAGCGAAAUCGCCGAAGAACUGGAAUUGCCGCCGGUAAAAAUCCAC  UGCUCCAUCUUGGCUGAAGAUGCGGUAAAAGCGGCCGUUGCCGACUACCGCAA  ACGUCAGGAAAACAGAUAA

cDNA: SEQ ID NO: 257

TTATCTGTTTTCCTGACGTTTGCGGTAGTCGGCAACGGCCGCTTTTACCGCATCTT  CAGCCAAGATGGAGCAGTGGATTTTTACCGGCGGCAATTCCAGTTCTTCGGCGAT  TTCGCTGTTTTTGATTGCCAGCGCGTCATCCAGACTTTTGCCTTTGACCCACTCGG  TAATCAGGCTGGACGAAGCGATGGCGGAACCGCAGCCGTAAGTTTTGAATTTCG  CATCTTCGATGATGCCTTCATCGTTCACTTTGATTTGCAGGCGCATCACGTCGCCG  CAGGCGGGCGCGCCGACCATGCCGGTGCCGACGGACTCGTCGTTTTTGTCGAAA  GTGCCGACGTTGCGGGGATTTTCGTAGTGGTCGATTACTTTATCGCTGTATGCCA   T

10. NGO0678: NC_002946.2:c667233-666979

DNA (- strand): SEQ ID NO: 258

ATGAACAAACTTTTCGTTACCGCCCTGTCCGCCCTCGCCTTGTCCGCCTGCGCCG  GCACTTGGCAGGGCGCGAAACAAGACACCGCCCGCAACCTTGACAAAACACAG  GCCGCCGCCGAACGCGCCGCCGAACAAACAGGCAACGCCGTCGAAAAAGGTTG  GGACAAAACCAAAGAAGCCGTCAAAAAAGGCGGCAATGCCGTCGGACGCGGCA  TTTCCCATCTCGGCAAAAAAATCGAAAACGCCACCGAATAA

RNA SEQ ID NO: 259

AUGAACAAACUUUUCGUUACCGCCCUGUCCGCCCUCGCCUUGUCCGCCUGCGC  CGGCACUUGGCAGGGCGCGAAACAAGACACCGCCCGCAACCUUGACAAAACAC  AGGCCGCCGCCGAACGCGCCGCCGAACAAACAGGCAACGCCGUCGAAAAAGGU  UGGGACAAAACCAAAGAAGCCGUCAAAAAAGGCGGCAAUGCCGUCGGACGCG  GCAUUUCCCAUCUCGGCAAAAAAAUCGAAAACGCCACCGAAUAA

cDNA: SEQ ID NO: 260

TTATTCGGTGGCGTTTTCGATTTTTTTGCCGAGATGGGAAATGCCGCGTCCGACG  GCATTGCCGCCTTTTTTGACGGCTTCTTTGGTTTTGTCCCAACCTTTTTCGACGGC  GTTGCCTGTTTGTTCGGCGGCGCGTTCGGCGGCGGCCTGTGTTTTGTCAAGGTTG  CGGGCGGTGTCTTGTTTCGCGCCCTGCCAAGTGCCGGCGCAGGCGGACAAGGCG  AGGGCGGACAGGGCGGTAACGAAAAGTTTGTTCAT

11. NGO0926: NC_002946.2:c906814-906077

DNA (- strand): SEQ ID NO: 261

ATGGCTTTGCAAGATCGTACCGGTCAAAAAGTACCTTCCGTAGTATTCCGCACCC  GCGTCGGCGACACTTGGAAAGATGTGTCTACCGATGATTTGTTCAAAGGCAAAA  AAGTAGTCGTATTCTCCCTGCCCGGTGCATTTACCCCGACTTGTTCTTCTTCACAC  CTGCCGCGTTACAACGAATTGTTCGGCGCGTTCAAAGAAAACGGCGTTGACGCA  ATCTGCTGCGTATCTGTAAACGATACTTTCGTAATGAACGCTTGGGCTGCCGAAG  AAGAATCAGACAACATCTACATGATTCCTGACGGCAACGGCGAATTTACCGAAG  GTATGGGTATGCTGGTCGGTAAAGAAGACTTGGGCTTCGGCAAACGCTCTTGGC  GTTACTCCATGCTGGTTAACGACGGCGTGGTTGAAAAAATGTTCATCGAACCTGA  AGAACCGGGCGATCCTTTCAAAGTATCCGATGCAGATACTATGCTGAAATTCGTT  GCTCCCGATTGGAAGGCTCAAGAGTCTGTGGCAATTTTCACTAAACCAGGTTGCC  AATTCTGTGCCAAAGTCAAACAAGCTTTGCAAGACAAAGGTTTGTCTTACGAAG  AAATCGTATTGGGCAAAGATGCAACCGTTACTTCCGTTCGCGCTATTACCGGCAA  GATGACTGCCCCTCAAGTCTTCATCGGCGGCAAATACATCGGCGGCAGCGAAGA  TTTGGAAGCTTACTTGGCTAAAAACTGA

RNA: SEQ ID NO: 262

AUGGCUUUGCAAGAUCGUACCGGUCAAAAAGUACCUUCCGUAGUAUUCCGCA  CCCGCGUCGGCGACACUUGGAAAGAUGUGUCUACCGAUGAUUUGUUCAAAGG  CAAAAAAGUAGUCGUAUUCUCCCUGCCCGGUGCAUUUACCCCGACUUGUUCUU  CUUCACACCUGCCGCGUUACAACGAAUUGUUCGGCGCGUUCAAAGAAAACGGC  GUUGACGCAAUCUGCUGCGUAUCUGUAAACGAUACUUUCGUAAUGAACGCUU  GGGCUGCCGAAGAAGAAUCAGACAACAUCUACAUGAUUCCUGACGGCAACGG  CGAAUUUACCGAAGGUAUGGGUAUGCUGGUCGGUAAAGAAGACUUGGGCUUC  GGCAAACGCUCUUGGCGUUACUCCAUGCUGGUUAACGACGGCGUGGUUGAAA  AAAUGUUCAUCGAACCUGAAGAACCGGGCGAUCCUUUCAAAGUAUCCGAUGC  AGAUACUAUGCUGAAAUUCGUUGCUCCCGAUUGGAAGGCUCAAGAGUCUGUG  GCAAUUUUCACUAAACCAGGUUGCCAAUUCUGUGCCAAAGUCAAACAAGCUU  UGCAAGACAAAGGUUUGUCUUACGAAGAAAUCGUAUUGGGCAAAGAUGCAAC  CGUUACUUCCGUUCGCGCUAUUACCGGCAAGAUGACUGCCCCUCAAGUCUUCA  UCGGCGGCAAAUACAUCGGCGGCAGCGAAGAUUUGGAAGCUUACUUGGCUAA   AAACUGA

cDNA: SEQ ID NO: 263

TCAGTTTTTAGCCAAGTAAGCTTCCAAATCTTCGCTGCCGCCGATGTATTTGCCG  CCGATGAAGACTTGAGGGGCAGTCATCTTGCCGGTAATAGCGCGAACGGAAGTA  ACGGTTGCATCTTTGCCCAATACGATTTCTTCGTAAGACAAACCTTTGTCTTGCA  AAGCTTGTTTGACTTTGGCACAGAATTGGCAACCTGGTTTAGTGAAAATTGCCAC  AGACTCTTGAGCCTTCCAATCGGGAGCAACGAATTTCAGCATAGTATCTGCATCG  GATACTTTGAAAGGATCGCCCGGTTCTTCAGGTTCGATGAACATTTTTTCAACCA  CGCCGTCGTTAACCAGCATGGAGTAACGCCAAGAGCGTTTGCCGAAGCCCAAGT  CTTCTTTACCGACCAGCATACCCATACCTTCGGTAAATTCGCCGTTGCCGTCAGG  AATCATGTAGATGTTGTCTGATTCTTCTTCGGCAGCCCAAGCGTTCATTACGAAA  GTATCGTTTACAGATACGCAGCAGATTGCGTCAACGCCGTTTTCTTTGAACGCGC  CGAACAATTCGTTGTAACGCGGCAGGTGTGAAGAAGAACAAGTCGGGGTAAATG  CACCGGGCAGGGAGAATACGACTACTTTTTTGCCTTTGAACAAATCATCGGTAGA  CACATCTTTCCAAGTGTCGCCGACGCGGGTGCGGAATACTACGGAAGGTACTTTT  TGACCGGTACGATCTTGCAAAGCCAT

12. NGO0936: NC_002946.2:c914813-914253

DNA (- strand): SEQ ID NO: 264

ATGAAAACAGCACAAGAACTGCGCGCCGGCAATGTATTTATGGTCGGCAACGAT  CCTATGGTCGTTCAAAAAACCGAATACATCAAAGGCGGCCGCTCTTCCGCCAAA  GTCAGCATGAAACTGAAAAACCTGCTGACCGGCGCTGCTTCCGAAACCATTTAC  AAAGCCGACGACAAATTCGACGTGGTCATCCTGTCCCGCAAAAACTGTACGTAC  AGCTATTTTGCCGACCCGATGTACGTCTTTATGGACGAAGAATTCAACCAATACG  AAATCGAAGCCGACAACATCGGCGACGCGTTGAAATTCATCGTTGACGGTATGG  AAGACCAATGCGAAGTTACCTTCTATGAAGGCAATCCCATTTCTGTCGAACTGCC  CACCATCATCGTGCGCGAAGTCGAGTACACCGAGCCTGCCGTCAAAGGCGATAC  TTCCGGCAAAGTGATGAAAACCGCGCGTCTGGTCGGCGGCACCGAAATCCAAGT  GATGTCTTACATCGAAAACGGCGACAAAGTCGAAATCGATACCCGTACCGGCGA  ATTCCGCAAACGCGCCTGA

RNA: SEQ ID NO: 265

AUGAAAACAGCACAAGAACUGCGCGCCGGCAAUGUAUUUAUGGUCGGCAACG  AUCCUAUGGUCGUUCAAAAAACCGAAUACAUCAAAGGCGGCCGCUCUUCCGCC  AAAGUCAGCAUGAAACUGAAAAACCUGCUGACCGGCGCUGCUUCCGAAACCAU  UUACAAAGCCGACGACAAAUUCGACGUGGUCAUCCUGUCCCGCAAAAACUGUA  CGUACAGCUAUUUUGCCGACCCGAUGUACGUCUUUAUGGACGAAGAAUUCAA  CCAAUACGAAAUCGAAGCCGACAACAUCGGCGACGCGUUGAAAUUCAUCGUU  GACGGUAUGGAAGACCAAUGCGAAGUUACCUUCUAUGAAGGCAAUCCCAUUU  CUGUCGAACUGCCCACCAUCAUCGUGCGCGAAGUCGAGUACACCGAGCCUGCC  GUCAAAGGCGAUACUUCCGGCAAAGUGAUGAAAACCGCGCGUCUGGUCGGCG  GCACCGAAAUCCAAGUGAUGUCUUACAUCGAAAACGGCGACAAAGUCGAAAU  CGAUACCCGUACCGGCGAAUUCCGCAAACGCGCCUGA

cDNA: SEQ ID NO: 266

TCAGGCGCGTTTGCGGAATTCGCCGGTACGGGTATCGATTTCGACTTTGTCGCCG  TTTTCGATGTAAGACATCACTTGGATTTCGGTGCCGCCGACCAGACGCGCGGTTT  TCATCACTTTGCCGGAAGTATCGCCTTTGACGGCAGGCTCGGTGTACTCGACTTC  GCGCACGATGATGGTGGGCAGTTCGACAGAAATGGGATTGCCTTCATAGAAGGT  AACTTCGCATTGGTCTTCCATACCGTCAACGATGAATTTCAACGCGTCGCCGATG  TTGTCGGCTTCGATTTCGTATTGGTTGAATTCTTCGTCCATAAAGACGTACATCGG  GTCGGCAAAATAGCTGTACGTACAGTTTTTGCGGGACAGGATGACCACGTCGAA  TTTGTCGTCGGCTTTGTAAATGGTTTCGGAAGCAGCGCCGGTCAGCAGGTTTTTC  AGTTTCATGCTGACTTTGGCGGAAGAGCGGCCGCCTTTGATGTATTCGGTTTTTT  GAACGACCATAGGATCGTTGCCGACCATAAATACATTGCCGGCGCGCAGTTCTT   GTGCTGTTTTCAT

13. NGO0950a: NC_002946.2:925084-925782

DNA (+ strand): SEQ ID NO: 267

GTGCAGGCGGATTTAGCCTACGCCGCCGAACGCATTACCCACGATTATCCGGAA  CCAACCGGTGCAAAAAAAGACAAAAAAATAAGCACGGTAAGCGATTATTTCAG  AAACATCCGTACGCATTCCGTCCACCCCAGGGTGTCGGTCGGCTACGATTTCGGC  AGCTGGAGGATAGCGGCAGATTATGCCCGTTACAGAAAGTGGAACAACAGTAAA  TATTCCGTCAACATAAAAAGGGTGAAAGAAAACAATGGCAGCGGGAAAAAACT  GACGCAAGACCTGAAGACGGAAAATCAGGAAAACGGTACGTTCCACGCCGTTTC  TTCTCTCGGCTTGTCCGCCGTTTACGATTTCGATACCGGTTCCCGCTTCAAACCCT  ATGCAGGCGTGCGCGTCAGCTACGGACACGTCAGACACAGCATCGATTCGACCA  AAAAAACAACAGATGTTATTACCGCCCCCCCCACTACTTCTGACGGAGCACCTA  CAACTTATAATGCTAATCCACAGACGCAAAACCCTTATCACCAAAGCGACAGCA  TCCGCCGCGTGGGCCTCGGCGTCATCGCCGGCGTCGGTTTCGACATCACGCCCAA  CCTGACCCTGGACACCGGCTACCGCTACCACAACTGGGGACGCCTGGAAAACAC  CCGCTTCAAAACCCACGAAGCCTCATTGGGCATGCGCTACCGCTTCTGA

RNA: SEQ ID NO: 268

GUGCAGGCGGAUUUAGCCUACGCCGCCGAACGCAUUACCCACGAUUAUCCGGA  ACCAACCGGUGCAAAAAAAGACAAAAAAAUAAGCACGGUAAGCGAUUAUUUC  AGAAACAUCCGUACGCAUUCCGUCCACCCCAGGGUGUCGGUCGGCUACGAUUU  CGGCAGCUGGAGGAUAGCGGCAGAUUAUGCCCGUUACAGAAAGUGGAACAAC  AGUAAAUAUUCCGUCAACAUAAAAAGGGUGAAAGAAAACAAUGGCAGCGGGA  AAAAACUGACGCAAGACCUGAAGACGGAAAAUCAGGAAAACGGUACGUUCCA  CGCCGUUUCUUCUCUCGGCUUGUCCGCCGUUUACGAUUUCGAUACCGGUUCCC  GCUUCAAACCCUAUGCAGGCGUGCGCGUCAGCUACGGACACGUCAGACACAGC  AUCGAUUCGACCAAAAAAACAACAGAUGUUAUUACCGCCCCCCCCACUACUUC  UGACGGAGCACCUACAACUUAUAAUGCUAAUCCACAGACGCAAAACCCUUAUC  ACCAAAGCGACAGCAUCCGCCGCGUGGGCCUCGGCGUCAUCGCCGGCGUCGGU  UUCGACAUCACGCCCAACCUGACCCUGGACACCGGCUACCGCUACCACAACUG  GGGACGCCUGGAAAACACCCGCUUCAAAACCCACGAAGCCUCAUUGGGCAUGC   GCUACCGCUUCUGA

cDNA: SEQ ID NO: 269

TCAGAAGCGGTAGCGCATGCCCAATGAGGCTTCGTGGGTTTTGAAGCGGGTGTTT  TCCAGGCGTCCCCAGTTGTGGTAGCGGTAGCCGGTGTCCAGGGTCAGGTTGGGC  GTGATGTCGAAACCGACGCCGGCGATGACGCCGAGGCCCACGCGGCGGATGCTG  TCGCTTTGGTGATAAGGGTTTTGCGTCTGTGGATTAGCATTATAAGTTGTAGGTG  CTCCGTCAGAAGTAGTGGGGGGGGCGGTAATAACATCTGTTGTTTTTTTGGTCGA  ATCGATGCTGTGTCTGACGTGTCCGTAGCTGACGCGCACGCCTGCATAGGGTTTG  AAGCGGGAACCGGTATCGAAATCGTAAACGGCGGACAAGCCGAGAGAAGAAAC  GGCGTGGAACGTACCGTTTTCCTGATTTTCCGTCTTCAGGTCTTGCGTCAGTTTTT  TCCCGCTGCCATTGTTTTCTTTCACCCTTTTTATGTTGACGGAATATTTACTGTTGT  TCCACTTTCTGTAACGGGCATAATCTGCCGCTATCCTCCAGCTGCCGAAATCGTA  GCCGACCGACACCCTGGGGTGGACGGAATGCGTACGGATGTTTCTGAAATAATC  GCTTACCGTGCTTATTTTTTTGTCTTTTTTTGCACCGGTTGGTTCCGGATAATCGT  GGGTAATGCGTTCGGCGGCGTAGGCTAAATCCGCCTGCAC

14. NGO1040a: NC_002946.2:c1000440-999760

DNA (- strand): SEQ ID NO: 270

GTGCAGGCGGATCTGGCTTACGCCTACGAGCACATCACCCGCGATTATCCCGAT  GCAGCCGGTGCAAACCAAGGCAAAAAAATAAGCACGGTAAGCGATTATTTCAAA  AACATCCGTACGCATTCCATCCACCCCAGGGTGTCGGTCGGCTACGACTTCGGCG  GCTGGAGGATAGCGGCAGATTATGCCCGTTACAGAAAGTGGAACGACAATAAAT  ATTCCGTCGACATAAAAGAGTTGGAAAACAAGAATCAGAATAAGAGAGACCTG  AAGACGGAAAATCAGGAAAACGGCAGCTTCCACGCCGTTTCTTCTCTCGGCTTAT  CAGCCGTTTACGATTTCAAACTCAACGACAAATTCAAACCCTATATCGGTGCGCG  CGTCGCCTACGGACACGTCAGACACAGCATCGATTCGACCAAAAAAACAACAGA  GTTTCTTACCGCCGCCGGTCAGGATGGCGGAGCGCCTACGGTTTATAATAACGGA  AGTACGCAAGACGCCCATCAAGAAAGCGACAGCATCCGCCGCGTGGGCCTCGGC  GTCATCGCCGGTATCGGTTTCGACATCACGCCCAAGCTGACCCTGGACACCGGCT  ACCGCTACCACAACTGGGGACGCTTGGAAAACACCCGCTTCAAAACCCACGAAG  CCTCATTGGGCGTGCGCTACCGCTTCTGA

RNA: SEQ ID NO: 271

GUGCAGGCGGAUCUGGCUUACGCCUACGAGCACAUCACCCGCGAUUAUCCCGA  UGCAGCCGGUGCAAACCAAGGCAAAAAAAUAAGCACGGUAAGCGAUUAUUUC  AAAAACAUCCGUACGCAUUCCAUCCACCCCAGGGUGUCGGUCGGCUACGACUU  CGGCGGCUGGAGGAUAGCGGCAGAUUAUGCCCGUUACAGAAAGUGGAACGAC  AAUAAAUAUUCCGUCGACAUAAAAGAGUUGGAAAACAAGAAUCAGAAUAAGA  GAGACCUGAAGACGGAAAAUCAGGAAAACGGCAGCUUCCACGCCGUUUCUUC  UCUCGGCUUAUCAGCCGUUUACGAUUUCAAACUCAACGACAAAUUCAAACCCU  AUAUCGGUGCGCGCGUCGCCUACGGACACGUCAGACACAGCAUCGAUUCGACC  AAAAAAACAACAGAGUUUCUUACCGCCGCCGGUCAGGAUGGCGGAGCGCCUAC  GGUUUAUAAUAACGGAAGUACGCAAGACGCCCAUCAAGAAAGCGACAGCAUC  CGCCGCGUGGGCCUCGGCGUCAUCGCCGGUAUCGGUUUCGACAUCACGCCCAA  GCUGACCCUGGACACCGGCUACCGCUACCACAACUGGGGACGCUUGGAAAACA  CCCGCUUCAAAACCCACGAAGCCUCAUUGGGCGUGCGCUACCGCUUCUGA

cDNA: SEQ ID NO: 272

TCAGAAGCGGTAGCGCACGCCCAATGAGGCTTCGTGGGTTTTGAAGCGGGTGTT  TTCCAAGCGTCCCCAGTTGTGGTAGCGGTAGCCGGTGTCCAGGGTCAGCTTGGGC  GTGATGTCGAAACCGATACCGGCGATGACGCCGAGGCCCACGCGGCGGATGCTG  TCGCTTTCTTGATGGGCGTCTTGCGTACTTCCGTTATTATAAACCGTAGGCGCTCC  GCCATCCTGACCGGCGGCGGTAAGAAACTCTGTTGTTTTTTTGGTCGAATCGATG  CTGTGTCTGACGTGTCCGTAGGCGACGCGCGCACCGATATAGGGTTTGAATTTGT  CGTTGAGTTTGAAATCGTAAACGGCTGATAAGCCGAGAGAAGAAACGGCGTGGA  AGCTGCCGTTTTCCTGATTTTCCGTCTTCAGGTCTCTCTTATTCTGATTCTTGTTTT  CCAACTCTTTTATGTCGACGGAATATTTATTGTCGTTCCACTTTCTGTAACGGGCA  TAATCTGCCGCTATCCTCCAGCCGCCGAAGTCGTAGCCGACCGACACCCTGGGGT  GGATGGAATGCGTACGGATGTTTTTGAAATAATCGCTTACCGTGCTTATTTTTTTG  CCTTGGTTTGCACCGGCTGCATCGGGATAATCGCGGGTGATGTGCTCGTAGGCGT  AAGCCAGATCCGCCTGCAC

15. NGO1073a: NC_002946.2:1035309-1035998

DNA (+ strand): SEQ ID NO: 273

GTGCAGGCGGATTTAGCCTACGCCGCCGAACGCATTACCCACGATTATCCGGAA  CCAACCGGTACAAAAAAAGACAAAATAAGCACGGTAAGCGATTATTTCAGAAAC  ATCCGTACGCATTCCATCCACCCCAGGGTGTCGGTCGGCTACGACTTCGGCGGCT  GGAGGATAGCGGCAGATTATGCCCGTTACAGAAAGTGGAACAACAGTAAATATT  CCGTCAACACAAAAAAGGTGAACGAAAACAAGGGCGAAAAGATAAACGTGACG  CAATATCTGAAGGCGGAAAATCAGGAAAACGGTACGTTCCACGCCGTTTCTTCTC  TCGGCTTGTCCGCCGTTTACGATTTCAAACTCAACGACAAATTCAAACCCTATAT  CGGTGCGCGCGTCGCCTACGGACACGTCAGACACAGCATCGATTCGACCAAAAA  AACAACAGAGTTTCTTACCGCCGCCGGTCAGGATGGCGGAGCGCCTACGGTTTA  TAATAACGGAAGTACGCAAGACGCCCATCAAGAAAGCGACAGCATCCGCCGCGT  GGGCCTCGGCGTCATCGCCGGCGTCGGTTTCGACATCACGCCCAACCTGACCTTG  GACGCCGGGTACCGCTACCACAACTGGGGACGCTTGGAAAACACCCGCTTCAAA  ACCCACGAAGCCTCGTTGGGCATGCGCTACCGCTTCTGA

RNA: SEQ ID NO: 274

GUGCAGGCGGAUUUAGCCUACGCCGCCGAACGCAUUACCCACGAUUAUCCGGA  ACCAACCGGUACAAAAAAAGACAAAAUAAGCACGGUAAGCGAUUAUUUCAGA  AACAUCCGUACGCAUUCCAUCCACCCCAGGGUGUCGGUCGGCUACGACUUCGG  CGGCUGGAGGAUAGCGGCAGAUUAUGCCCGUUACAGAAAGUGGAACAACAGU  AAAUAUUCCGUCAACACAAAAAAGGUGAACGAAAACAAGGGCGAAAAGAUAA  ACGUGACGCAAUAUCUGAAGGCGGAAAAUCAGGAAAACGGUACGUUCCACGC  CGUUUCUUCUCUCGGCUUGUCCGCCGUUUACGAUUUCAAACUCAACGACAAAU  UCAAACCCUAUAUCGGUGCGCGCGUCGCCUACGGACACGUCAGACACAGCAUC  GAUUCGACCAAAAAAACAACAGAGUUUCUUACCGCCGCCGGUCAGGAUGGCG  GAGCGCCUACGGUUUAUAAUAACGGAAGUACGCAAGACGCCCAUCAAGAAAG  CGACAGCAUCCGCCGCGUGGGCCUCGGCGUCAUCGCCGGCGUCGGUUUCGACA  UCACGCCCAACCUGACCUUGGACGCCGGGUACCGCUACCACAACUGGGGACGC  UUGGAAAACACCCGCUUCAAAACCCACGAAGCCUCGUUGGGCAUGCGCUACCG   CUUCUGA

cDNA: SEQ ID NO: 275

TCAGAAGCGGTAGCGCATGCCCAACGAGGCTTCGTGGGTTTTGAAGCGGGTGTT  TTCCAAGCGTCCCCAGTTGTGGTAGCGGTACCCGGCGTCCAAGGTCAGGTTGGG  CGTGATGTCGAAACCGACGCCGGCGATGACGCCGAGGCCCACGCGGCGGATGCT  GTCGCTTTCTTGATGGGCGTCTTGCGTACTTCCGTTATTATAAACCGTAGGCGCTC  CGCCATCCTGACCGGCGGCGGTAAGAAACTCTGTTGTTTTTTTGGTCGAATCGAT  GCTGTGTCTGACGTGTCCGTAGGCGACGCGCGCACCGATATAGGGTTTGAATTTG  TCGTTGAGTTTGAAATCGTAAACGGCGGACAAGCCGAGAGAAGAAACGGCGTGG  AACGTACCGTTTTCCTGATTTTCCGCCTTCAGATATTGCGTCACGTTTATCTTTTC  GCCCTTGTTTTCGTTCACCTTTTTTGTGTTGACGGAATATTTACTGTTGTTCCACTT  TCTGTAACGGGCATAATCTGCCGCTATCCTCCAGCCGCCGAAGTCGTAGCCGACC  GACACCCTGGGGTGGATGGAATGCGTACGGATGTTTCTGAAATAATCGCTTACC  GTGCTTATTTTGTCTTTTTTTGTACCGGTTGGTTCCGGATAATCGTGGGTAATGCG  TTCGGCGGCGTAGGCTAAATCCGCCTGCAC

16. NGO1225: NC_002946.2:c1175547-1174729

DNA (- strand): SEQ ID NO: 276

ATGAACACCATTTTCAAAATCAGCGCACTGACCCTTTCCGCCGCTTTGGCACTTT  CCGCCTGCGGCAAAAAAGAAGCCGCCCCCGCATCTGCATCCGAACCTGCCGCCG  CTTCTGCCGCGCAGGGCGACACCTCTTCAATCGGCAGCACGATGCAGCAGGCAA  GCTATGCAATGGGCGTGGACATCGGACGCTCCCTGAAACAAATGAAGGAACAGG  GCGCGGAAATCGATTTGAAAGTCTTTACCGATGCCATGCAGGCAGTGTATGACG  GCAAAGAAATCAAAATGACCGAAGAGCAGGCCCAGGAAGTGATGATGAAATTC  CTGCAGGAGCAGCAGGCTAAAGCCGTAGAAAAACACAAGGCGGATGCGAAGGC  CAACAAAGAAAAAGGCGAAGCCTTCCTGAAGGAAAATGCCGCCAAAGACGGCG  TGAAGACCACTGCTTCCGGTCTGCAGTACAAAATCACCAAACAGGGTGAAGGCA  AACAGCCGACAAAAGACGACATCGTTACCGTGGAATACGAAGGCCGCCTGATTG  ACGGTACCGTATTCGACAGCAGCAAAGCCAACGGCGGCCCGGCCACCTTCCCTT  TGAGCCAAGTGATTCCGGGTTGGACCGAAGGCGTACGGCTTCTGAAAGAAGGCG  GCGAAGCCACGTTCTACATCCCGTCCAACCTTGCCTACCGCGAACAGGGTGCGG  GCGAAAAAATCGGTCCGAACGCCACTTTGGTATTTGACGTGAAACTGGTCAAAA  TCGGCGCACCCGAAAACGCGCCCGCCAAGCAGCCGGATCAAGTCGACATCAAAA   AAGTAAATTAA

RNA: SEQ ID NO: 277

AUGAACACCAUUUUCAAAAUCAGCGCACUGACCCUUUCCGCCGCUUUGGCACU  UUCCGCCUGCGGCAAAAAAGAAGCCGCCCCCGCAUCUGCAUCCGAACCUGCCG  CCGCUUCUGCCGCGCAGGGCGACACCUCUUCAAUCGGCAGCACGAUGCAGCAG  GCAAGCUAUGCAAUGGGCGUGGACAUCGGACGCUCCCUGAAACAAAUGAAGG  AACAGGGCGCGGAAAUCGAUUUGAAAGUCUUUACCGAUGCCAUGCAGGCAGU  GUAUGACGGCAAAGAAAUCAAAAUGACCGAAGAGCAGGCCCAGGAAGUGAUG  AUGAAAUUCCUGCAGGAGCAGCAGGCUAAAGCCGUAGAAAAACACAAGGCGG  AUGCGAAGGCCAACAAAGAAAAAGGCGAAGCCUUCCUGAAGGAAAAUGCCGC  CAAAGACGGCGUGAAGACCACUGCUUCCGGUCUGCAGUACAAAAUCACCAAAC  AGGGUGAAGGCAAACAGCCGACAAAAGACGACAUCGUUACCGUGGAAUACGA  AGGCCGCCUGAUUGACGGUACCGUAUUCGACAGCAGCAAAGCCAACGGCGGCC  CGGCCACCUUCCCUUUGAGCCAAGUGAUUCCGGGUUGGACCGAAGGCGUACGG  CUUCUGAAAGAAGGCGGCGAAGCCACGUUCUACAUCCCGUCCAACCUUGCCUA  CCGCGAACAGGGUGCGGGCGAAAAAAUCGGUCCGAACGCCACUUUGGUAUUU  GACGUGAAACUGGUCAAAAUCGGCGCACCCGAAAACGCGCCCGCCAAGCAGCC  GGAUCAAGUCGACAUCAAAAAAGUAAAUUAA

cDNA: SEQ ID NO: 278

TTAATTTACTTTTTTGATGTCGACTTGATCCGGCTGCTTGGCGGGCGCGTTTTCGG  GTGCGCCGATTTTGACCAGTTTCACGTCAAATACCAAAGTGGCGTTCGGACCGAT  TTTTTCGCCCGCACCCTGTTCGCGGTAGGCAAGGTTGGACGGGATGTAGAACGTG  GCTTCGCCGCCTTCTTTCAGAAGCCGTACGCCTTCGGTCCAACCCGGAATCACTT  GGCTCAAAGGGAAGGTGGCCGGGCCGCCGTTGGCTTTGCTGCTGTCGAATACGG  TACCGTCAATCAGGCGGCCTTCGTATTCCACGGTAACGATGTCGTCTTTTGTCGG  CTGTTTGCCTTCACCCTGTTTGGTGATTTTGTACTGCAGACCGGAAGCAGTGGTCT  TCACGCCGTCTTTGGCGGCATTTTCCTTCAGGAAGGCTTCGCCTTTTTCTTTGTTG  GCCTTCGCATCCGCCTTGTGTTTTTCTACGGCTTTAGCCTGCTGCTCCTGCAGGAA  TTTCATCATCACTTCCTGGGCCTGCTCTTCGGTCATTTTGATTTCTTTGCCGTCATA  CACTGCCTGCATGGCATCGGTAAAGACTTTCAAATCGATTTCCGCGCCCTGTTCC  TTCATTTGTTTCAGGGAGCGTCCGATGTCCACGCCCATTGCATAGCTTGCCTGCT  GCATCGTGCTGCCGATTGAAGAGGTGTCGCCCTGCGCGGCAGAAGCGGCGGCAG  GTTCGGATGCAGATGCGGGGGCGGCTTCTTTTTTGCCGCAGGCGGAAAGTGCCA  AAGCGGCGGAAAGGGTCAGTGCGCTGATTTTGAAAATGGTGTTCAT

17. NGO1277a: NC_002946.2:1231620-1232324

DNA (+ strand): SEQ ID NO: 279

GTGCAGGCGGATTTAGCCTACGCCGCCGAACGCATTACCCACGATTATCCGGAA  CCAACCGGTGCAAAAAAAGGCAAAATAAGCACGGTAAGCGATTATTTCAGAAAC  ATCCGTACGCATTCCATCCACCCCAGGGTGTCGGTCGGCTACGACTTCGGCGGCT  GGAGGATAGCGGCAGATTATGCCCGTTACAGAAAGTGGAACGACAATAAATATT  CCGTGAACATAAAAGAGTTGGGAAGAAAGGATGGTACCTCTTCTAGCGGCCGCT  ATCTTAACATACAAACCCGAAAGACGGAAAATCAGGAAAACGGTACGTTCCACG  CCGTTTCTTCTCTCGGCTTGTCAACCGTTTACGATTTCAGAGCCAACGATAAATTC  AAACCCTATATCGGCGTGCGCGTCGCCTACGGACACGTCAGACATCAGGTTCATT  CAATGGAAAAAGAAACCACGACTGTTACCACTTACCCAAGCGACGGTAGTGCGA  AAACTTCTGTTCCATCAGAAATGCCCCCCAAACCTGCCTATCACGAAAACCGCA  GCAGCCGCCGCTTGGGCTTCGGCGCGATGGCGGGCGTGGGCATAGACGTCGCGC  CCGGTCTGACCTTGGACGCCGGCTACCGCTACCACTATTGGGGACGCCTGGAAA  ACACCCGCTTCAAAACCCACGAAGCCTCATTGGGCATGCGCTACCGCTTCTGA

RNA: SEQ ID NO: 280

GUGCAGGCGGAUUUAGCCUACGCCGCCGAACGCAUUACCCACGAUUAUCCGGA  ACCAACCGGUGCAAAAAAAGGCAAAAUAAGCACGGUAAGCGAUUAUUUCAGA  AACAUCCGUACGCAUUCCAUCCACCCCAGGGUGUCGGUCGGCUACGACUUCGG  CGGCUGGAGGAUAGCGGCAGAUUAUGCCCGUUACAGAAAGUGGAACGACAAU  AAAUAUUCCGUGAACAUAAAAGAGUUGGGAAGAAAGGAUGGUACCUCUUCUA  GCGGCCGCUAUCUUAACAUACAAACCCGAAAGACGGAAAAUCAGGAAAACGG  UACGUUCCACGCCGUUUCUUCUCUCGGCUUGUCAACCGUUUACGAUUUCAGAG  CCAACGAUAAAUUCAAACCCUAUAUCGGCGUGCGCGUCGCCUACGGACACGUC  AGACAUCAGGUUCAUUCAAUGGAAAAAGAAACCACGACUGUUACCACUUACC  CAAGCGACGGUAGUGCGAAAACUUCUGUUCCAUCAGAAAUGCCCCCCAAACCU  GCCUAUCACGAAAACCGCAGCAGCCGCCGCUUGGGCUUCGGCGCGAUGGCGGG  CGUGGGCAUAGACGUCGCGCCCGGUCUGACCUUGGACGCCGGCUACCGCUACC  ACUAUUGGGGACGCCUGGAAAACACCCGCUUCAAAACCCACGAAGCCUCAUUG  GGCAUGCGCUACCGCUUCUGA

cDNA: SEQ ID NO: 281

TCAGAAGCGGTAGCGCATGCCCAATGAGGCTTCGTGGGTTTTGAAGCGGGTGTTT  TCCAGGCGTCCCCAATAGTGGTAGCGGTAGCCGGCGTCCAAGGTCAGACCGGGC  GCGACGTCTATGCCCACGCCCGCCATCGCGCCGAAGCCCAAGCGGCGGCTGCTG  CGGTTTTCGTGATAGGCAGGTTTGGGGGGCATTTCTGATGGAACAGAAGTTTTCG  CACTACCGTCGCTTGGGTAAGTGGTAACAGTCGTGGTTTCTTTTTCCATTGAATG  AACCTGATGTCTGACGTGTCCGTAGGCGACGCGCACGCCGATATAGGGTTTGAA  TTTATCGTTGGCTCTGAAATCGTAAACGGTTGACAAGCCGAGAGAAGAAACGGC  GTGGAACGTACCGTTTTCCTGATTTTCCGTCTTTCGGGTTTGTATGTTAAGATAGC  GGCCGCTAGAAGAGGTACCATCCTTTCTTCCCAACTCTTTTATGTTCACGGAATA  TTTATTGTCGTTCCACTTTCTGTAACGGGCATAATCTGCCGCTATCCTCCAGCCGC  CGAAGTCGTAGCCGACCGACACCCTGGGGTGGATGGAATGCGTACGGATGTTTC  TGAAATAATCGCTTACCGTGCTTATTTTGCCTTTTTTTGCACCGGTTGGTTCCGGA  TAATCGTGGGTAATGCGTTCGGCGGCGTAGGCTAAATCCGCCTGCAC

18. Ngo1513: Nc_002946.2:1481445-1482281

DNA (+ strand): SEQ ID NO: 282

ATGAATCCAGCCCGCAAAAAACCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT  CTTCTCTTCTCTTCTCTTCTCTTCTCTTCGGCAGCGCAGGCGGCAAGTGAAGGCAA  TGGCCGCGGCCCGTATGTGCAGGCGGATTTAGCCTACGCCGCCGAACGCATTAC  CCACGATTATCCGGAACCAACCGCTCCAGGCAAAAACAAAATAAGCACGGTAAG  CGATTATTTCAGAAACATCCGTACGCATTCCATCCACCCCAGGGTGTCGGTCGGC  TACGACTTCGGCGGCTGGCGCATCGCCGCGGATTATGCCCGTTACAGGAAATGG  CACAACAATAAATATTCCGTGAACATAAAAGAGTTGGAAAGAAAGAATAATAA  AACTTTTGGCGGCAACCAGCTTAACATAAAATACCAAAAGACGGAACATCAGGA  AAACGGCACATTCCACGCCGTTTCTTCTCTCGGCTTGTCCGCCGTTTACGATTTCA  AACTCAACGACAAATTCAAACCCTATATCGGTGCGCGCGTCGCCTACGGACACG  TCAGACACAGCATCGATTCGACTAAAAAAATAACAGGTACTCTTACCGCCTACC  CTAGTGATGCTGACGCAGCAGTTACGGTTTATCCTGACGGACATCCGCAAAAAA  ACACCTATCAAAAAAGCAACAGCAGCCGCCGCTTGGGCTTCGGCGCGATGGCGG  GCGTGGGCATAGACGTCGCGCCCGGCCTGACCTTGGACGCCGGCTACCGCTACC  ACAACTGGGGACGCTTGGAAAACACCCGCTTCAAAACCCACGAAGCCTCGTTGG  GCATGCGCTACCGCTTCTGA

RNA: SEQ ID NO: 283

AUGAAUCCAGCCCGCAAAAAACCUUCUCUUCUCUUCUCUUCUCUUCUCUUCUC  UUCUCUUCUCUUCUCUUCUCUUCUCUUCUCUUCGGCAGCGCAGGCGGCAAGUG  AAGGCAAUGGCCGCGGCCCGUAUGUGCAGGCGGAUUUAGCCUACGCCGCCGAA  CGCAUUACCCACGAUUAUCCGGAACCAACCGCUCCAGGCAAAAACAAAAUAAG  CACGGUAAGCGAUUAUUUCAGAAACAUCCGUACGCAUUCCAUCCACCCCAGGG  UGUCGGUCGGCUACGACUUCGGCGGCUGGCGCAUCGCCGCGGAUUAUGCCCGU  UACAGGAAAUGGCACAACAAUAAAUAUUCCGUGAACAUAAAAGAGUUGGAAA  GAAAGAAUAAUAAAACUUUUGGCGGCAACCAGCUUAACAUAAAAUACCAAAA  GACGGAACAUCAGGAAAACGGCACAUUCCACGCCGUUUCUUCUCUCGGCUUGU  CCGCCGUUUACGAUUUCAAACUCAACGACAAAUUCAAACCCUAUAUCGGUGCG  CGCGUCGCCUACGGACACGUCAGACACAGCAUCGAUUCGACUAAAAAAAUAAC  AGGUACUCUUACCGCCUACCCUAGUGAUGCUGACGCAGCAGUUACGGUUUAUC  CUGACGGACAUCCGCAAAAAAACACCUAUCAAAAAAGCAACAGCAGCCGCCGC  UUGGGCUUCGGCGCGAUGGCGGGCGUGGGCAUAGACGUCGCGCCCGGCCUGAC  CUUGGACGCCGGCUACCGCUACCACAACUGGGGACGCUUGGAAAACACCCGCU  UCAAAACCCACGAAGCCUCGUUGGGCAUGCGCUACCGCUUCUGA

cDNA: SEQ ID NO: 284

TCAGAAGCGGTAGCGCATGCCCAACGAGGCTTCGTGGGTTTTGAAGCGGGTGTT  TTCCAAGCGTCCCCAGTTGTGGTAGCGGTAGCCGGCGTCCAAGGTCAGGCCGGG  CGCGACGTCTATGCCCACGCCCGCCATCGCGCCGAAGCCCAAGCGGCGGCTGCT  GTTGCTTTTTTGATAGGTGTTTTTTTGCGGATGTCCGTCAGGATAAACCGTAACTG  CTGCGTCAGCATCACTAGGGTAGGCGGTAAGAGTACCTGTTATTTTTTTAGTCGA  ATCGATGCTGTGTCTGACGTGTCCGTAGGCGACGCGCGCACCGATATAGGGTTTG  AATTTGTCGTTGAGTTTGAAATCGTAAACGGCGGACAAGCCGAGAGAAGAAACG  GCGTGGAATGTGCCGTTTTCCTGATGTTCCGTCTTTTGGTATTTTATGTTAAGCTG  GTTGCCGCCAAAAGTTTTATTATTCTTTCTTTCCAACTCTTTTATGTTCACGGAAT  ATTTATTGTTGTGCCATTTCCTGTAACGGGCATAATCCGCGGCGATGCGCCAGCC  GCCGAAGTCGTAGCCGACCGACACCCTGGGGTGGATGGAATGCGTACGGATGTT  TCTGAAATAATCGCTTACCGTGCTTATTTTGTTTTTGCCTGGAGCGGTTGGTTCCG  GATAATCGTGGGTAATGCGTTCGGCGGCGTAGGCTAAATCCGCCTGCACATACG  GGCCGCGGCCATTGCCTTCACTTGCCGCCTGCGCTGCCGAAGAGAAGAGAAGAG  AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGGTTT  TTTGCGGGCTGGATTCAT

19. NGO1553a: NC_002946.2:1531422-1532120

DNA (+ strand): SEQ ID NO: 285

GTGCAGGCGGATTTAGCCTACGCCGCCGAACGCATTACCCACGATTATCCGGAA  CCAACCGGTGCAAAAAAAGACAAAAAAATAAGCACGGTAAGCGATTATTTCAG  AAACATCCGTACGCATTCCGTCCACCCCAGGGTGTCGGTCGGCTACGATTTCGGC  AGCTGGAGGATAGCGGCAGATTATGCCCGTTACAGAAAGTGGAACAACAGTAAA  TATTCCGTCAACATAAAAAGGGTGAAAGAAAACAATGGCAGCGGGAAAAAACT  GACGCAAGACCTGAAGACGGAAAATCAGGAAAACGGTACGTTCCACGCCGTTTC  TTCTCTCGGCTTGTCCGCCGTTTACGATTTCGATACCGGTTCCCGCTTCAAACCCT  ATGCAGGCGTGCGCGTCAGCTACGGACACGTCAGACACAGCATCGATTCGACCA  AAAAAACAACAGATGTTATTACCGCCCCCCCCACTACTTCTGACGGAGCACCTA  CAACTTATAATGCTAATCCACAGACGCAAAACCCTTATCACCAAAGCGACAGCA  TCCGCCGCGTGGGCCTCGGCGTCATCGCCGGCGTCGGTTTCGACATCACGCCCAA  CCTGACCCTGGACACCGGCTACCGCTACCACAACTGGGGACGCCTGGAAAACAC  CCGCTTCAAAACCCACGAAGCCTCATTGGGCATGCGCTACCGCTTCTGA

RNA: SEQ ID NO: 286

GUGCAGGCGGAUUUAGCCUACGCCGCCGAACGCAUUACCCACGAUUAUCCGGA  ACCAACCGGUGCAAAAAAAGACAAAAAAAUAAGCACGGUAAGCGAUUAUUUC  AGAAACAUCCGUACGCAUUCCGUCCACCCCAGGGUGUCGGUCGGCUACGAUUU  CGGCAGCUGGAGGAUAGCGGCAGAUUAUGCCCGUUACAGAAAGUGGAACAAC  AGUAAAUAUUCCGUCAACAUAAAAAGGGUGAAAGAAAACAAUGGCAGCGGGA  AAAAACUGACGCAAGACCUGAAGACGGAAAAUCAGGAAAACGGUACGUUCCA  CGCCGUUUCUUCUCUCGGCUUGUCCGCCGUUUACGAUUUCGAUACCGGUUCCC  GCUUCAAACCCUAUGCAGGCGUGCGCGUCAGCUACGGACACGUCAGACACAGC  AUCGAUUCGACCAAAAAAACAACAGAUGUUAUUACCGCCCCCCCCACUACUUC  UGACGGAGCACCUACAACUUAUAAUGCUAAUCCACAGACGCAAAACCCUUAUC  ACCAAAGCGACAGCAUCCGCCGCGUGGGCCUCGGCGUCAUCGCCGGCGUCGGU  UUCGACAUCACGCCCAACCUGACCCUGGACACCGGCUACCGCUACCACAACUG  GGGACGCCUGGAAAACACCCGCUUCAAAACCCACGAAGCCUCAUUGGGCAUGC   GCUACCGCUUCUGA

cDNA: SEQ ID NO: 287

TCAGAAGCGGTAGCGCATGCCCAATGAGGCTTCGTGGGTTTTGAAGCGGGTGTTT  TCCAGGCGTCCCCAGTTGTGGTAGCGGTAGCCGGTGTCCAGGGTCAGGTTGGGC  GTGATGTCGAAACCGACGCCGGCGATGACGCCGAGGCCCACGCGGCGGATGCTG  TCGCTTTGGTGATAAGGGTTTTGCGTCTGTGGATTAGCATTATAAGTTGTAGGTG  CTCCGTCAGAAGTAGTGGGGGGGGCGGTAATAACATCTGTTGTTTTTTTGGTCGA  ATCGATGCTGTGTCTGACGTGTCCGTAGCTGACGCGCACGCCTGCATAGGGTTTG  AAGCGGGAACCGGTATCGAAATCGTAAACGGCGGACAAGCCGAGAGAAGAAAC  GGCGTGGAACGTACCGTTTTCCTGATTTTCCGTCTTCAGGTCTTGCGTCAGTTTTT  TCCCGCTGCCATTGTTTTCTTTCACCCTTTTTATGTTGACGGAATATTTACTGTTGT  TCCACTTTCTGTAACGGGCATAATCTGCCGCTATCCTCCAGCTGCCGAAATCGTA  GCCGACCGACACCCTGGGGTGGACGGAATGCGTACGGATGTTTCTGAAATAATC  GCTTACCGTGCTTATTTTTTTGTCTTTTTTTGCACCGGTTGGTTCCGGATAATCGT  GGGTAATGCGTTCGGCGGCGTAGGCTAAATCCGCCTGCAC

20. Ngo1762: Nc_002946.2:1724401-1724637

DNA (+ strand): SEQ ID NO: 288

ATGTCAAACATCGAACAACAAGTTAAGAAAATTATTGCTGAACAACTGGGCGTA  AACGAAGCCGACGTGAAAAACGAATCTTCCTTCCAAGACGACTTGGGCGCGGAT  TCTTTGGATACCGTGGAGTTGGTTATGGCTTTGGAAGAAGCCTTCGGCTGCGAAA  TCCCCGACGAAGATGCCGAAAAAATCACCACCGTCCAACTGGCTATCGACTACA  TCAATGCCCACAACGGCTAA

RNA: SEQ ID NO: 289

AUGUCAAACAUCGAACAACAAGUUAAGAAAAUUAUUGCUGAACAACUGGGCG  UAAACGAAGCCGACGUGAAAAACGAAUCUUCCUUCCAAGACGACUUGGGCGC  GGAUUCUUUGGAUACCGUGGAGUUGGUUAUGGCUUUGGAAGAAGCCUUCGGC  UGCGAAAUCCCCGACGAAGAUGCCGAAAAAAUCACCACCGUCCAACUGGCUAU  CGACUACAUCAAUGCCCACAACGGCUAA

cDNA: SEQ ID NO: 290

TTAGCCGTTGTGGGCATTGATGTAGTCGATAGCCAGTTGGACGGTGGTGATTTTT  TCGGCATCTTCGTCGGGGATTTCGCAGCCGAAGGCTTCTTCCAAAGCCATAACCA  ACTCCACGGTATCCAAAGAATCCGCGCCCAAGTCGTCTTGGAAGGAAGATTCGT  TTTTCACGTCGGCTTCGTTTACGCCCAGTTGTTCAGCAATAATTTTCTTAACTTGT  TGTTCGATGTTTGACAT

21. NGO1842: NC_002946.2:c1808872-1807688

DNA (- strand): SEQ ID NO: 291

ATGGCTAAGGAAAAATTCGAACGTAGCAAACCGCACGTAAACGTTGGCACCATC  GGTCACGTTGACCATGGTAAAACCACCCTGACTGCTGCTTTGACTACTATTTTAG  CTAAAAAATTCGGCGGCGCTGCAAAAGCTTACGACCAAATCGACAACGCACCCG  AAGAAAAAGCACGCGGTATTACCATTAACACCTCGCACGTAGAATACGAAACCG  AAACCCGCCACTACGCACACGTAGACTGTCCGGGTCACGCCGACTACGTTAAAA  ACATGATTACCGGCGCCGCACAAATGGACGGTGCAATCCTGGTATGTTCTGCTGC  CGACGGCCCTATGCCGCAAACCCGCGAACACATCCTGCTGGCCCGTCAAGTAGG  CGTACCTTACATCATCGTGTTCATGAACAAATGCGACATGGTCGACGATGCCGAG  CTGTTGGAACTGGTTGAAATGGAAATCCGCGACCTGCTGTCCAGCTACGACTTCC  CCGGCGACGACTGCCCGATCGTACAAGGTTCCGCACTGAAAGCCTTGGAAGGCG  ATGCCGCTTACGAAGAAAAAATCTTCGAACTGGCTACCGCATTGGACAGCTACA  TCCCGACTCCCGAGCGTGCCGTGGACAAACCATTCCTGCTGCCTATCGAAGACGT  GTTCTCCATTTCCGGCCGCGGTACCGTAGTCACCGGCCGTGTAGAGCGAGGTATC  ATCCACGTTGGTGACGAGATTGAAATCGTCGGTCTGAAAGAAACCCAAAAAACC  ACCTGTACCGGCGTTGAAATGTTCCGCAAACTGCTGGACGAAGGTCAGGCGGGC  GACAACGTAGGCGTATTGCTGCGCGGTACCAAACGTGAAGACGTAGAACGCGGT  CAGGTATTGGCCAAACCGGGTACTATCACTCCTCACACCAAGTTCAAAGCAGAA  GTGTACGTATTGAGCAAAGAAGAGGGCGGCCGCCATACCCCGTTTTTCGCCAAC  TACCGTCCCCAATTCTACTTCCGTACCACTGACGTAACCGGCGCGGTTACTTTGG  AAAAAGGTGTGGAAATGGTAATGCCGGGTGAGAACGTAACCATTACTGTAGAAC  TGATTGCGCCTATCGCTATGGAAGAAGGTCTGCGCTTTGCGATTCGCGAAGGCGG  CCGTACCGTGGGTGCCGGCGTGGTTTCTTCTGTTATCGCTTAA

RNA: SEQ ID NO: 292

AUGGCUAAGGAAAAAUUCGAACGUAGCAAACCGCACGUAAACGUUGGCACCA  UCGGUCACGUUGACCAUGGUAAAACCACCCUGACUGCUGCUUUGACUACUAUU  UUAGCUAAAAAAUUCGGCGGCGCUGCAAAAGCUUACGACCAAAUCGACAACG  CACCCGAAGAAAAAGCACGCGGUAUUACCAUUAACACCUCGCACGUAGAAUAC  GAAACCGAAACCCGCCACUACGCACACGUAGACUGUCCGGGUCACGCCGACUA  CGUUAAAAACAUGAUUACCGGCGCCGCACAAAUGGACGGUGCAAUCCUGGUA  UGUUCUGCUGCCGACGGCCCUAUGCCGCAAACCCGCGAACACAUCCUGCUGGC  CCGUCAAGUAGGCGUACCUUACAUCAUCGUGUUCAUGAACAAAUGCGACAUG  GUCGACGAUGCCGAGCUGUUGGAACUGGUUGAAAUGGAAAUCCGCGACCUGC  UGUCCAGCUACGACUUCCCCGGCGACGACUGCCCGAUCGUACAAGGUUCCGCA  CUGAAAGCCUUGGAAGGCGAUGCCGCUUACGAAGAAAAAAUCUUCGAACUGG  CUACCGCAUUGGACAGCUACAUCCCGACUCCCGAGCGUGCCGUGGACAAACCA  UUCCUGCUGCCUAUCGAAGACGUGUUCUCCAUUUCCGGCCGCGGUACCGUAGU  CACCGGCCGUGUAGAGCGAGGUAUCAUCCACGUUGGUGACGAGAUUGAAAUC  GUCGGUCUGAAAGAAACCCAAAAAACCACCUGUACCGGCGUUGAAAUGUUCC  GCAAACUGCUGGACGAAGGUCAGGCGGGCGACAACGUAGGCGUAUUGCUGCG  CGGUACCAAACGUGAAGACGUAGAACGCGGUCAGGUAUUGGCCAAACCGGGU  ACUAUCACUCCUCACACCAAGUUCAAAGCAGAAGUGUACGUAUUGAGCAAAG  AAGAGGGCGGCCGCCAUACCCCGUUUUUCGCCAACUACCGUCCCCAAUUCUAC  UUCCGUACCACUGACGUAACCGGCGCGGUUACUUUGGAAAAAGGUGUGGAAA  UGGUAAUGCCGGGUGAGAACGUAACCAUUACUGUAGAACUGAUUGCGCCUAU  CGCUAUGGAAGAAGGUCUGCGCUUUGCGAUUCGCGAAGGCGGCCGUACCGUG  GGUGCCGGCGUGGUUUCUUCUGUUAUCGCUUAA

cDNA: SEQ ID NO: 293

TTAAGCGATAACAGAAGAAACCACGCCGGCACCCACGGTACGGCCGCCTTCGCG  AATCGCAAAGCGCAGACCTTCTTCCATAGCGATAGGCGCAATCAGTTCTACAGT  AATGGTTACGTTCTCACCCGGCATTACCATTTCCACACCTTTTTCCAAAGTAACC  GCGCCGGTTACGTCAGTGGTACGGAAGTAGAATTGGGGACGGTAGTTGGCGAAA  AACGGGGTATGGCGGCCGCCCTCTTCTTTGCTCAATACGTACACTTCTGCTTTGA  ACTTGGTGTGAGGAGTGATAGTACCCGGTTTGGCCAATACCTGACCGCGTTCTAC  GTCTTCACGTTTGGTACCGCGCAGCAATACGCCTACGTTGTCGCCCGCCTGACCT  TCGTCCAGCAGTTTGCGGAACATTTCAACGCCGGTACAGGTGGTTTTTTGGGTTT  CTTTCAGACCGACGATTTCAATCTCGTCACCAACGTGGATGATACCTCGCTCTAC  ACGGCCGGTGACTACGGTACCGCGGCCGGAAATGGAGAACACGTCTTCGATAGG  CAGCAGGAATGGTTTGTCCACGGCACGCTCGGGAGTCGGGATGTAGCTGTCCAA  TGCGGTAGCCAGTTCGAAGATTTTTTCTTCGTAAGCGGCATCGCCTTCCAAGGCT  TTCAGTGCGGAACCTTGTACGATCGGGCAGTCGTCGCCGGGGAAGTCGTAGCTG  GACAGCAGGTCGCGGATTTCCATTTCAACCAGTTCCAACAGCTCGGCATCGTCGA  CCATGTCGCATTTGTTCATGAACACGATGATGTAAGGTACGCCTACTTGACGGGC  CAGCAGGATGTGTTCGCGGGTTTGCGGCATAGGGCCGTCGGCAGCAGAACATAC  CAGGATTGCACCGTCCATTTGTGCGGCGCCGGTAATCATGTTTTTAACGTAGTCG  GCGTGACCCGGACAGTCTACGTGTGCGTAGTGGCGGGTTTCGGTTTCGTATTCTA  CGTGCGAGGTGTTAATGGTAATACCGCGTGCTTTTTCTTCGGGTGCGTTGTCGAT  TTGGTCGTAAGCTTTTGCAGCGCCGCCGAATTTTTTAGCTAAAATAGTAGTCAAA  GCAGCAGTCAGGGTGGTTTTACCATGGTCAACGTGACCGATGGTGCCAACGTTTA  CGTGCGGTTTGCTACGTTCGAATTTTTCCTTAGCCAT

22. NGO1871: NC_002946.2:c1842986-1842483

DNA (- strand): SEQ ID NO: 294

ATGGCTTTACTGAATATCTTGCAATATCCCGACGAGCGTCTGCACACGGTGGCAA  AGCCTGTCGAACAAGTTGACGAGCGCATCCGGAAGCTGGTTGCCGATATGTTTG  AAACGATGTACGAATCGCGCGGCATCGGGCTGGCGGCGACGCAGGTCGATGTGC  ACGAACGCGTGGTCGTGATGGATTTGACCGAAGACCGCAGCGAACCGCGCGTGT  TCATCAACCCCGTCATCGTTGAAAAAGACGGCGAAACCACTTACGAAGAGGGCT  GCCTGTCCGTACCGGGCATTTACGACGCCGTTACCCGCGCCGAACGCGTCAAGG  TCGAGGCTTTGAACGAAAAAGGCGAAAAATTCACGCTGGAGGCGGACGGGCTGC  TGGCGATTTGCGTGCAGCACGAGTTAGATCACCTGATGGGCATCGTGTTTGTCGA  ACGCCTTTCCCAACTCAAGCAGGGGCGGATTAAGACCAAACTGAAAAAACGTCA  GAAACATACGATTTGA

RNA: SEQ ID NO: 295

AUGGCUUUACUGAAUAUCUUGCAAUAUCCCGACGAGCGUCUGCACACGGUGG  CAAAGCCUGUCGAACAAGUUGACGAGCGCAUCCGGAAGCUGGUUGCCGAUAU  GUUUGAAACGAUGUACGAAUCGCGCGGCAUCGGGCUGGCGGCGACGCAGGUC  GAUGUGCACGAACGCGUGGUCGUGAUGGAUUUGACCGAAGACCGCAGCGAAC  CGCGCGUGUUCAUCAACCCCGUCAUCGUUGAAAAAGACGGCGAAACCACUUAC  GAAGAGGGCUGCCUGUCCGUACCGGGCAUUUACGACGCCGUUACCCGCGCCGA  ACGCGUCAAGGUCGAGGCUUUGAACGAAAAAGGCGAAAAAUUCACGCUGGAG  GCGGACGGGCUGCUGGCGAUUUGCGUGCAGCACGAGUUAGAUCACCUGAUGG  GCAUCGUGUUUGUCGAACGCCUUUCCCAACUCAAGCAGGGGCGGAUUAAGACC  AAACUGAAAAAACGUCAGAAACAUACGAUUUGA

cDNA: SEQ ID NO: 296

TCAAATCGTATGTTTCTGACGTTTTTTCAGTTTGGTCTTAATCCGCCCCTGCTTGA  GTTGGGAAAGGCGTTCGACAAACACGATGCCCATCAGGTGATCTAACTCGTGCT  GCACGCAAATCGCCAGCAGCCCGTCCGCCTCCAGCGTGAATTTTTCGCCTTTTTC  GTTCAAAGCCTCGACCTTGACGCGTTCGGCGCGGGTAACGGCGTCGTAAATGCC  CGGTACGGACAGGCAGCCCTCTTCGTAAGTGGTTTCGCCGTCTTTTTCAACGATG  ACGGGGTTGATGAACACGCGCGGTTCGCTGCGGTCTTCGGTCAAATCCATCACG  ACCACGCGTTCGTGCACATCGACCTGCGTCGCCGCCAGCCCGATGCCGCGCGATT  CGTACATCGTTTCAAACATATCGGCAACCAGCTTCCGGATGCGCTCGTCAACTTG  TTCGACAGGCTTTGCCACCGTGTGCAGACGCTCGTCGGGATATTGCAAGATATTC   AGTAAAGCCAT

23. Ngo1908: Nc_002946.2:1881198-1882241

DNA (+ strand): SEQ ID NO: 297

ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTC  ACCTGAGTTCGGGCATATCCCCTATGATTCGGGTTCACGGCGACATGCGGCGCAT  CAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGCAATATGGTAACTTCGGTGAT  GAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTT  CGAACTGCCCAACGTCGCCCGATTCCGCGTCAACGCCTTCAACACCGGCCGCGG  CCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGAAGAATTG  AAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTG  GTTACCGGCCCTACCGGTTCGGGCAAATCGACCACGCTTGCCGCGATGATCAACT  ACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGACCCGATCGAAT  TCGTCCACCAAAGCAAAAAATCCCTGATTAACCAACGCGAGCTGCACCAGCACA  CCCTCAGCTTCGCCAACGCGCTGAGTTCCGCATTGCGCGAAGACCCCGACGTTAT  CCTTGTCGGCGAGATGCGCGACCCCGAAACCATCGGCTTGGCACTGACCGCCGC  CGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCGGCAAAAAC  CGTCGACCGTATCGTGGACGTATTCCCCGCCGGAGAGAAAGAAATGGTGCGTTC  CATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAACCTGCTGAAAACGCAC  GACGGCGACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTC  CGCAACCTCATCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACC  GGGCGGGCGAGCGGTATGCAGACGATGGACCAATCGCTGCAATCGCTGGTGCGC  CAAGGGCTGATCGCACCGGAAGCCACACGCAGACGCGCGCAAAACAGCGAAAG   TATGAGTTTCTGA

RNA: SEQ ID NO: 298

AUGCAGAUUACCGACUUACUCGCCUUCGGCGCUAAAAACAAAGCAUCCGACCU  UCACCUGAGUUCGGGCAUAUCCCCUAUGAUUCGGGUUCACGGCGACAUGCGGC  GCAUCAACCUUCCCGAAAUGAGCGCGGAAGAGGUCGGCAAUAUGGUAACUUC  GGUGAUGAACGACCACCAGCGGAAAAUCUACCAGCAAAACUUGGAAGUCGAC  UUCUCGUUCGAACUGCCCAACGUCGCCCGAUUCCGCGUCAACGCCUUCAACAC  CGGCCGCGGCCCCGCCGCCGUAUUCCGCACCAUUCCCAGCACCGUCUUAUCGC  UGGAAGAAUUGAAAGCCCCGAGCAUUUUCCAAAAAAUCGCAGAAUCGCCGCG  CGGCAUGGUAUUGGUUACCGGCCCUACCGGUUCGGGCAAAUCGACCACGCUUG  CCGCGAUGAUCAACUACAUCAACGAAACCCAGCCGGCACACAUCCUGACCAUC  GAAGACCCGAUCGAAUUCGUCCACCAAAGCAAAAAAUCCCUGAUUAACCAACG  CGAGCUGCACCAGCACACCCUCAGCUUCGCCAACGCGCUGAGUUCCGCAUUGC  GCGAAGACCCCGACGUUAUCCUUGUCGGCGAGAUGCGCGACCCCGAAACCAUC  GGCUUGGCACUGACCGCCGCCGAAACCGGACACUUGGUUUUCGGCACGCUGCA  CACGACCGGCGCGGCAAAAACCGUCGACCGUAUCGUGGACGUAUUCCCCGCCG  GAGAGAAAGAAAUGGUGCGUUCCAUGCUGUCCGAAUCGCUGACCGCCGUCAU  CUCCCAAAACCUGCUGAAAACGCACGACGGCGACGGCCGUGUCGCCUCGCACG  AAAUCCUGAUUGCCAACCCCGCCGUCCGCAACCUCAUCCGCGAAAACAAAAUC  ACGCAGAUUAACUCCGUCCUGCAAACCGGGCGGGCGAGCGGUAUGCAGACGAU  GGACCAAUCGCUGCAAUCGCUGGUGCGCCAAGGGCUGAUCGCACCGGAAGCCA  CACGCAGACGCGCGCAAAACAGCGAAAGUAUGAGUUUCUGA

cDNA: SEQ ID NO: 299

TCAGAAACTCATACTTTCGCTGTTTTGCGCGCGTCTGCGTGTGGCTTCCGGTGCG  ATCAGCCCTTGGCGCACCAGCGATTGCAGCGATTGGTCCATCGTCTGCATACCGC  TCGCCCGCCCGGTTTGCAGGACGGAGTTAATCTGCGTGATTTTGTTTTCGCGGAT  GAGGTTGCGGACGGCGGGGTTGGCAATCAGGATTTCGTGCGAGGCGACACGGCC  GTCGCCGTCGTGCGTTTTCAGCAGGTTTTGGGAGATGACGGCGGTCAGCGATTCG  GACAGCATGGAACGCACCATTTCTTTCTCTCCGGCGGGGAATACGTCCACGATAC  GGTCGACGGTTTTTGCCGCGCCGGTCGTGTGCAGCGTGCCGAAAACCAAGTGTC  CGGTTTCGGCGGCGGTCAGTGCCAAGCCGATGGTTTCGGGGTCGCGCATCTCGCC  GACAAGGATAACGTCGGGGTCTTCGCGCAATGCGGAACTCAGCGCGTTGGCGAA  GCTGAGGGTGTGCTGGTGCAGCTCGCGTTGGTTAATCAGGGATTTTTTGCTTTGG  TGGACGAATTCGATCGGGTCTTCGATGGTCAGGATGTGTGCCGGCTGGGTTTCGT  TGATGTAGTTGATCATCGCGGCAAGCGTGGTCGATTTGCCCGAACCGGTAGGGC  CGGTAACCAATACCATGCCGCGCGGCGATTCTGCGATTTTTTGGAAAATGCTCGG  GGCTTTCAATTCTTCCAGCGATAAGACGGTGCTGGGAATGGTGCGGAATACGGC  GGCGGGGCCGCGGCCGGTGTTGAAGGCGTTGACGCGGAATCGGGCGACGTTGGG  CAGTTCGAACGAGAAGTCGACTTCCAAGTTTTGCTGGTAGATTTTCCGCTGGTGG  TCGTTCATCACCGAAGTTACCATATTGCCGACCTCTTCCGCGCTCATTTCGGGAA  GGTTGATGCGCCGCATGTCGCCGTGAACCCGAATCATAGGGGATATGCCCGAAC  TCAGGTGAAGGTCGGATGCTTTGTTTTTAGCGCCGAAGGCGAGTAAGTCGGTAAT   CTGCAT

24. NGO1982: NC_002946.2:c1957797-1957498

DNA (- strand): SEQ ID NO: 300

ATGAAAATATTTGAAAATATAGAAGATGTTAAAGCCATCCGTAAAAAGACCGGG  ATGAACCAGATAGACTTCTGGGGCAAGGTCGGCGTTACTCAATCCGGAGGTTCA  CGCTACGAAACCGGCCGTAAGATGCCCAAACCCGTACGCGAACTGCTCCGCCTC  GTCCATATCGAATGCCTCGATTTGGCAAAAGTCAACAAAAAAGATATGGAAATC  GCCGCCCTGTTGAAAAAACACCATCCCGACCTGTATGCCGAGTTGTCCAAACAG  ACCAAGTCCGAAAGAAAAAAACAAAGTTAA

RNA: SEQ ID NO: 301

AUGAAAAUAUUUGAAAAUAUAGAAGAUGUUAAAGCCAUCCGUAAAAAGACCG  GGAUGAACCAGAUAGACUUCUGGGGCAAGGUCGGCGUUACUCAAUCCGGAGG  UUCACGCUACGAAACCGGCCGUAAGAUGCCCAAACCCGUACGCGAACUGCUCC  GCCUCGUCCAUAUCGAAUGCCUCGAUUUGGCAAAAGUCAACAAAAAAGAUAU  GGAAAUCGCCGCCCUGUUGAAAAAACACCAUCCCGACCUGUAUGCCGAGUUGU  CCAAACAGACCAAGUCCGAAAGAAAAAAACAAAGUUAA

cDNA: SEQ ID NO: 302

TTAACTTTGTTTTTTTCTTTCGGACTTGGTCTGTTTGGACAACTCGGCATACAGGT  CGGGATGGTGTTTTTTCAACAGGGCGGCGATTTCCATATCTTTTTTGTTGACTTTT  GCCAAATCGAGGCATTCGATATGGACGAGGCGGAGCAGTTCGCGTACGGGTTTG  GGCATCTTACGGCCGGTTTCGTAGCGTGAACCTCCGGATTGAGTAACGCCGACCT  TGCCCCAGAAGTCTATCTGGTTCATCCCGGTCTTTTTACGGATGGCTTTAACATCT  TCTATATTTTCAAATATTTTCAT

25. NGO2060a: NC_002946.2:c2037067-2036384

DNA (- strand): SEQ ID NO: 303

GTGCAGGCGGATTTAGCCTACGCCGCCGAACGCATTACCCACGATTATCCGGAA  CCAACCGCTCCAGGCAAAAACAAAATAAGCACGGTAAGCGATTATTTCAGAAAC  ATCCGTACGCATTCCATCCACCCCAGGGTGTCGGTCGGCTACGACTTCGGCGGCT  GGAGGATAGCGGCAGATTATGCCCGTTACAGAAAGTGGAACGACAATAAATATT  CCGTCGACATAAAAGAGTTGGAAAACAAGAATCAGAATAAGAGAGACCTGAAG  ACGGAAAATCAGGAAAACGGCAGCTTCCACGCCGTTTCTTCTCTCGGCTTATCAG  CCGTTTACGATTTCAAACTCAACGACAAATTCAAACCCTATATCGGTGCGCGCGT  CGCCTACGGACACGTCAGACACAGCATCGATTCGACTAAAAAAATAACAGGTAC  TCTTACCGCCTACCCTAGTGATGCTGACGCAGCAGTTACGGTTTATCCTGACGGA  CATCCGCAAAAAAACACCTATCAAAAAAGCAACAGCAGCCGCCGCTTGGGCTTC  GGCGCGATGGCGGGCGTGGGCATAGACGTCGCGCCCGGCCTGACCTTGGACGCC  GGCTACCGCTACCACAACTGGGGACGCTTGGAAAACACCCGCTTCAAAACCCAC  GAAGCCTCATTGGGCATGCGCTACCGCTTCTGA

RNA: SEQ ID NO: 304

GUGCAGGCGGAUUUAGCCUACGCCGCCGAACGCAUUACCCACGAUUAUCCGGA  ACCAACCGCUCCAGGCAAAAACAAAAUAAGCACGGUAAGCGAUUAUUUCAGA  AACAUCCGUACGCAUUCCAUCCACCCCAGGGUGUCGGUCGGCUACGACUUCGG  CGGCUGGAGGAUAGCGGCAGAUUAUGCCCGUUACAGAAAGUGGAACGACAAU  AAAUAUUCCGUCGACAUAAAAGAGUUGGAAAACAAGAAUCAGAAUAAGAGAG  ACCUGAAGACGGAAAAUCAGGAAAACGGCAGCUUCCACGCCGUUUCUUCUCUC  GGCUUAUCAGCCGUUUACGAUUUCAAACUCAACGACAAAUUCAAACCCUAUA  UCGGUGCGCGCGUCGCCUACGGACACGUCAGACACAGCAUCGAUUCGACUAAA  AAAAUAACAGGUACUCUUACCGCCUACCCUAGUGAUGCUGACGCAGCAGUUAC  GGUUUAUCCUGACGGACAUCCGCAAAAAAACACCUAUCAAAAAAGCAACAGC  AGCCGCCGCUUGGGCUUCGGCGCGAUGGCGGGCGUGGGCAUAGACGUCGCGCC  CGGCCUGACCUUGGACGCCGGCUACCGCUACCACAACUGGGGACGCUUGGAAA  ACACCCGCUUCAAAACCCACGAAGCCUCAUUGGGCAUGCGCUACCGCUUCUGA

cDNA: SEQ ID NO: 305

TCAGAAGCGGTAGCGCATGCCCAATGAGGCTTCGTGGGTTTTGAAGCGGGTGTTT  TCCAAGCGTCCCCAGTTGTGGTAGCGGTAGCCGGCGTCCAAGGTCAGGCCGGGC  GCGACGTCTATGCCCACGCCCGCCATCGCGCCGAAGCCCAAGCGGCGGCTGCTG  TTGCTTTTTTGATAGGTGTTTTTTTGCGGATGTCCGTCAGGATAAACCGTAACTGC  TGCGTCAGCATCACTAGGGTAGGCGGTAAGAGTACCTGTTATTTTTTTAGTCGAA  TCGATGCTGTGTCTGACGTGTCCGTAGGCGACGCGCGCACCGATATAGGGTTTGA  ATTTGTCGTTGAGTTTGAAATCGTAAACGGCTGATAAGCCGAGAGAAGAAACGG  CGTGGAAGCTGCCGTTTTCCTGATTTTCCGTCTTCAGGTCTCTCTTATTCTGATTC  TTGTTTTCCAACTCTTTTATGTCGACGGAATATTTATTGTCGTTCCACTTTCTGTA  ACGGGCATAATCTGCCGCTATCCTCCAGCCGCCGAAGTCGTAGCCGACCGACAC  CCTGGGGTGGATGGAATGCGTACGGATGTTTCTGAAATAATCGCTTACCGTGCTT  ATTTTGTTTTTGCCTGGAGCGGTTGGTTCCGGATAATCGTGGGTAATGCGTTCGG  CGGCGTAGGCTAAATCCGCCTGCAC

26. Ngo2084: Nc_002946.2:2061613-2062296

DNA (+ strand): SEQ ID NO: 306

ATGCAACACGACGTTTACGACTACACCGCGCATACGGTTTCTAAAAACACCGTC  CTGCAGAAAACCTACCGCCTGCTCGGATTTTCATTCATTCCGGCAGCCGCAGGCG  CGGCACTTGCCGCCAATGCCGGTTTCAATTTTTACGCCGCCTTCGGTTCGCGCTG  GATAGGATTTGCCGTCGTATTGGCGTTTTTCTACGGTATGATCCACTTCATCGAA  AAAAACCGTTACAGCAATACCGGCGTTACCCTGCTGATGGTATTCACATTCGGTA  TGGGCGTATTGATCGGCCCCGTGCTGCAATACGCACTCCATATTGCCGACGGTGC  GAAAATCGTCGGCATTGCCGCCGCGATGACCGCCGCCGTCTTTTTAACGATGTCC  GCATTGGCACGCCGAACCCGGCTCGATATGAACGCGCTCGGACGCTTCCTGACC  GTAGGTGCGGTCATTCTGATGGTCGCCGTGGTTGCCAATCTGTTTTTGGGTATTCC  CGCACTCGCCCTGACCATTTCCGCCGGTTTTGTCTTGTTCAGTTCCTTAATAATTA  TGTGGCAGGTACGCACCGTCATCGACGGCGGCGAAGACAGTTACATCAGCGCGG  CACTGACACTGTTTATCTCGCTTTACAACATCTTCAGCAGCCTGCTCAACATCCT  GCTGTCCTTAAACGGCGACGACTGA

RNA: SEQ ID NO: 307

AUGCAACACGACGUUUACGACUACACCGCGCAUACGGUUUCUAAAAACACCGU  CCUGCAGAAAACCUACCGCCUGCUCGGAUUUUCAUUCAUUCCGGCAGCCGCAG  GCGCGGCACUUGCCGCCAAUGCCGGUUUCAAUUUUUACGCCGCCUUCGGUUCG  CGCUGGAUAGGAUUUGCCGUCGUAUUGGCGUUUUUCUACGGUAUGAUCCACU  UCAUCGAAAAAAACCGUUACAGCAAUACCGGCGUUACCCUGCUGAUGGUAUU  CACAUUCGGUAUGGGCGUAUUGAUCGGCCCCGUGCUGCAAUACGCACUCCAUA  UUGCCGACGGUGCGAAAAUCGUCGGCAUUGCCGCCGCGAUGACCGCCGCCGUC  UUUUUAACGAUGUCCGCAUUGGCACGCCGAACCCGGCUCGAUAUGAACGCGCU  CGGACGCUUCCUGACCGUAGGUGCGGUCAUUCUGAUGGUCGCCGUGGUUGCCA  AUCUGUUUUUGGGUAUUCCCGCACUCGCCCUGACCAUUUCCGCCGGUUUUGUC  UUGUUCAGUUCCUUAAUAAUUAUGUGGCAGGUACGCACCGUCAUCGACGGCG  GCGAAGACAGUUACAUCAGCGCGGCACUGACACUGUUUAUCUCGCUUUACAAC  AUCUUCAGCAGCCUGCUCAACAUCCUGCUGUCCUUAAACGGCGACGACUGA

cDNA: SEQ ID NO: 308

TCAGTCGTCGCCGTTTAAGGACAGCAGGATGTTGAGCAGGCTGCTGAAGATGTT  GTAAAGCGAGATAAACAGTGTCAGTGCCGCGCTGATGTAACTGTCTTCGCCGCC  GTCGATGACGGTGCGTACCTGCCACATAATTATTAAGGAACTGAACAAGACAAA  ACCGGCGGAAATGGTCAGGGCGAGTGCGGGAATACCCAAAAACAGATTGGCAA  CCACGGCGACCATCAGAATGACCGCACCTACGGTCAGGAAGCGTCCGAGCGCGT  TCATATCGAGCCGGGTTCGGCGTGCCAATGCGGACATCGTTAAAAAGACGGCGG  CGGTCATCGCGGCGGCAATGCCGACGATTTTCGCACCGTCGGCAATATGGAGTG  CGTATTGCAGCACGGGGCCGATCAATACGCCCATACCGAATGTGAATACCATCA  GCAGGGTAACGCCGGTATTGCTGTAACGGTTTTTTTCGATGAAGTGGATCATACC  GTAGAAAAACGCCAATACGACGGCAAATCCTATCCAGCGCGAACCGAAGGCGG  CGTAAAAATTGAAACCGGCATTGGCGGCAAGTGCCGCGCCTGCGGCTGCCGGAA  TGAATGAAAATCCGAGCAGGCGGTAGGTTTTCTGCAGGACGGTGTTTTTAGAAA  CCGTATGCGCGGTGTAGTCGTAAACGTCGTGTTGCAT

27. NGO2134: NC_002946.2:c2114153-2113941

DNA (- strand): SEQ ID NO: 309

ATGCCTGCAATCCGCGTAAAAGAGAATGAACCATTTGAAGTCGCTATGCGCCGT  TTCAAACGCGCCGTAGAAAAAACCGGCCTGCTGACCGAGCTGCGCGCCCGCGAA  GCCTACGAAAAACCGACTACCGAACGCAAACGCAAAAAAGCGGCAGCCGTAAA  ACGCCTGCAAAAACGCCTGCGCAGCCAACAGCTGCCGCCCAAAATGTACTAA

RNA: SEQ ID NO: 310

AUGCCUGCAAUCCGCGUAAAAGAGAAUGAACCAUUUGAAGUCGCUAUGCGCC  GUUUCAAACGCGCCGUAGAAAAAACCGGCCUGCUGACCGAGCUGCGCGCCCGC  GAAGCCUACGAAAAACCGACUACCGAACGCAAACGCAAAAAAGCGGCAGCCGU  AAAACGCCUGCAAAAACGCCUGCGCAGCCAACAGCUGCCGCCCAAAAUGUACU   AA

cDNA: SEQ ID NO: 311

TTAGTACATTTTGGGCGGCAGCTGTTGGCTGCGCAGGCGTTTTTGCAGGCGTTTT  ACGGCTGCCGCTTTTTTGCGTTTGCGTTCGGTAGTCGGTTTTTCGTAGGCTTCGCG  GGCGCGCAGCTCGGTCAGCAGGCCGGTTTTTTCTACGGCGCGTTTGAAACGGCG  CATAGCGACTTCAAATGGTTCATTCTCTTTTACGCGGATTGCAGGCAT

28. Ngo2145: Nc_002946.2:2122709-2122945

DNA (+ strand): SEQ ID NO: 312

ATGGGTTTGATTGCTATCGCATGTGGTTTGATCGTTGCATTGGGTGCATTGGGTG  CATCTATCGGTATCGCAATGGTCGGTTCTAAATATTTGGAGTCTTCTGCTCGCCA  ACCTGAACTGATTGGTCCGCTGCAAACCAAACTGTTCCTGATTGCCGGTCTGATT  GATGCCGCATTCTTGATCGGTGTCGCCATTGCACTACTGTTCGCCTTCGTCAACC  CGTTTGCAGGTGCATAA

RNA: SEQ ID NO: 313

AUGGGUUUGAUUGCUAUCGCAUGUGGUUUGAUCGUUGCAUUGGGUGCAUUGG  GUGCAUCUAUCGGUAUCGCAAUGGUCGGUUCUAAAUAUUUGGAGUCUUCUGC  UCGCCAACCUGAACUGAUUGGUCCGCUGCAAACCAAACUGUUCCUGAUUGCCG  GUCUGAUUGAUGCCGCAUUCUUGAUCGGUGUCGCCAUUGCACUACUGUUCGCC  UUCGUCAACCCGUUUGCAGGUGCAUAA

cDNA: SEQ ID NO: 314

TTATGCACCTGCAAACGGGTTGACGAAGGCGAACAGTAGTGCAATGGCGACACC  GATCAAGAATGCGGCATCAATCAGACCGGCAATCAGGAACAGTTTGGTTTGCAG  CGGACCAATCAGTTCAGGTTGGCGAGCAGAAGACTCCAAATATTTAGAACCGAC  CATTGCGATACCGATAGATGCACCCAATGCACCCAATGCAACGATCAAACCACA  TGCGATAGCAATCAAACCCAT

29. Ngo2146: Nc_002946.2:2123015-2123485

DNA (+ strand): SEQ ID NO: 315

GTGAATATCAATGCAACATTATTCGCTCAAATCATCGTCTTTTTCGGTTTGGTATG  GTTTACCATGAAATTTGTGTGGCCGCCGATTGCAAAAGCTTTGGATGAGCGTGCC  GCAAAAATCGCCGAGGGCTTGGCTGCCGCCGAGCGTGGTAAAAGCGATTTCGAG  CAGGCTGAAAAAAAGGTTGCAGAACTTTTGGCAGAAGGGCGTAATCAGGTTTCC  GAAATGGTTGCCAACGCCGAAAAACGTGCCGCCAAAATTGTCGAAGAAGCCAA  AGAACAGGCTTCTTCCGAGGCGGCGCGCATTGCAGCTCAGGCAAAGGCCGATGT  GGAGCAGGAATTGTTCCGCGCACGCGAATCCCTGCGCGATCAGGTTGCCGTGTT  GGCTGTCAAAGGTGCCGAATCTATTTTGCGCAGCGAAGTCGATGCTTCCAAACAC  GCAAAACTGCTCGATACCCTGAAACAGGAGTTGTAA

RNA: SEQ ID NO: 316

GUGAAUAUCAAUGCAACAUUAUUCGCUCAAAUCAUCGUCUUUUUCGGUUUGG  UAUGGUUUACCAUGAAAUUUGUGUGGCCGCCGAUUGCAAAAGCUUUGGAUGA  GCGUGCCGCAAAAAUCGCCGAGGGCUUGGCUGCCGCCGAGCGUGGUAAAAGCG  AUUUCGAGCAGGCUGAAAAAAAGGUUGCAGAACUUUUGGCAGAAGGGCGUAA  UCAGGUUUCCGAAAUGGUUGCCAACGCCGAAAAACGUGCCGCCAAAAUUGUC  GAAGAAGCCAAAGAACAGGCUUCUUCCGAGGCGGCGCGCAUUGCAGCUCAGGC  AAAGGCCGAUGUGGAGCAGGAAUUGUUCCGCGCACGCGAAUCCCUGCGCGAUC  AGGUUGCCGUGUUGGCUGUCAAAGGUGCCGAAUCUAUUUUGCGCAGCGAAGU  CGAUGCUUCCAAACACGCAAAACUGCUCGAUACCCUGAAACAGGAGUUGUAA

cDNA: SEQ ID NO: 317

TTACAACTCCTGTTTCAGGGTATCGAGCAGTTTTGCGTGTTTGGAAGCATCGACT  TCGCTGCGCAAAATAGATTCGGCACCTTTGACAGCCAACACGGCAACCTGATCG  CGCAGGGATTCGCGTGCGCGGAACAATTCCTGCTCCACATCGGCCTTTGCCTGAG  CTGCAATGCGCGCCGCCTCGGAAGAAGCCTGTTCTTTGGCTTCTTCGACAATTTT  GGCGGCACGTTTTTCGGCGTTGGCAACCATTTCGGAAACCTGATTACGCCCTTCT  GCCAAAAGTTCTGCAACCTTTTTTTCAGCCTGCTCGAAATCGCTTTTACCACGCTC  GGCGGCAGCCAAGCCCTCGGCGATTTTTGCGGCACGCTCATCCAAAGCTTTTGCA  ATCGGCGGCCACACAAATTTCATGGTAAACCATACCAAACCGAAAAAGACGATG  ATTTGAGCGAATAATGTTGCATTGATATTCAC

tRNA Control Transcripts 1. NGO_t45: NC_002946.2:cl827200-1827128

DNA (- strand): SEQ ID NO: 318

AGGCCAATAGCTCAATTGGTAGAGTATCGGTCTCCAAAACCGAGGGTTGGGGGT  TCGAGACCCTCTTGGCCTG

RNA: SEQ ID NO: 319

AGGCCAAUAGCUCAAUUGGUAGAGUAUCGGUCUCCAAAACCGAGGGUUGGGG  GUUCGAGACCCUCUUGGCCUG

cDNA: SEQ ID NO: 320

CAGGCCAAGAGGGTCTCGAACCCCCAACCCTCGGTTTTGGAGACCGATACTCTA  CCAATTGAGCTATTGGCCT

2. NGO_t47: NC_002946.2:c1828597-1828527

DNA (- strand) SEQ ID NO: 321

GCGGGTGTAGCTCAATGGTAGAGCAGAAGCCTTCCAAGCTTACGGTGAGGGTTC  GATTCCCTTCACCCGCT

RNA: SEQ ID NO: 322

GCGGGUGUAGCUCAAUGGUAGAGCAGAAGCCUUCCAAGCUUACGGUGAGGGU  UCGAUUCCCUUCACCCGCU

cDNA: SEQ ID NO: 323

AGCGGGTGAAGGGAATCGAACCCTCACCGTAAGCTTGGAAGGCTTCTGCTCTAC  CATTGAGCTACACCCGC

Ribosomal RNA Control Transcripts 1. NGO_r02:NC_002946.2:c1119158-1116249

DNA (- strand): SEQ ID NO: 324

TGAAATGATAGAGTCAAGTGAATAAGTGCATCAGGCGGATGCCTTGGCGATGAT  AGGCGACGAAGGACGTGTAAGCCTGCGAAAAGCGCGGGGGAGCTGGCAATAAA  GCAATGATCCCGCGGTGTCCGAATGGGGAAACCCACTGCATTCTGTGCAGTATC  CTAAGTTGAATACATAGGCTTAGAGAAGCGAACCCGGAGAACTGAACCATCTAA  GTACCCGGAGGAAAAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAAC  GCGGAGGAGCCTGTACGTAATAACTGTCGAGGTAGAAGAACAAGCTGGGAAGCT  TGACCATAGCGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCG  TACGAAAAGTAGGGCGGGACACGTGAAATCCTGTCTGAATATGGGGGGACCATC  CTCCAAGGCTAAATACTCATCATCGACCGATAGTGAACCAGTACCGTGAGGGAA  AGGCGAAAAGAACCCCGGGAGGGGAGTGAAACAGAACCTGAAACCTGATGCAT  ACAAACAGTGGGAGCGCCCTAGTGGTGTGACTGCGTACCTTTTGTATAATGGGTC  AACGACTTACATTCAGTAGCGAGCTTAACCGGATAGGGGAGGCGTAGGGAAACC  GAGTCTTAATAGGGCGATGAGTTGCTGGGTGTAGACCCGAAACCGAGTGATCTA  TCCATGGCCAGGTTGAAGGTGCCGTAACAGGTACTGGAGGACCGAACCCACGCA  TGTTGCAAAATGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCTAAACAAACTC  GGAGATAGCTGGTTCTCCCCGAAAACTATTTAGGTAGTGCCTCGAGCAAGACAC  TGATGGGGGTAAAGCACTGTTATGGCTAGGGGGTTATTGCAACTTACCAACCCAT  GGCAAACTCAGAATACCATCAAGTGGTTCCTCGGGAGACAGACAGCGGGTGCTA  ACGTCCGTTGTCAAGAGGGAAACAACCCAGACCGCCGGCTAAGGTCCCAAATGA  TAGATTAAGTGGTAAACGAAGTGGGAAGGCACAGACAGCCAGGATGTTGGCTTA  GAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGTCCTGC  GCGGAAGATGTAACGGGGCTCAAATCTATAACCGAAGCTGCGGATGCCGGTTTA  CCGGCATGGTAGGGGAGCGTTCTGTAGGCTGATGAAGGTGCATTGTAAAGTGTG  CTGGAGGTATCAGAAGTGCGAATGTTGACATGAGTAGCGATAAAGCGGGTGAAA  AGCCCGCTCGCCGAAAGCCCAAGGTTTCCTACGCAACGTTCATCGGCGTAGGGT  GAGTCGGCCCCTAAGGCGAGGCAGAAATGCGTAGTCGATGGGAAACAGGTTAAT  ATTCCTGTACTTGATTCAAATGCGATGTGGGGACGGAGAAGGTTAGGTTGGCAA  GCTGTTGGAATAGCTTGTTTAAGCCGGTAGGTGGAAGACTTAGGCAAATCCGGG  TTTTCTTAACACCGAAGAAGTGATGACGAGTGTTTACGGACACGAAGCAACCGA  TACCACGCTTCCAGGAAAAGCCACTAAGCTTCAGTTTGAATCGAACCGTACCGC  AAACCGACACAGGTGGGCAGGATGAGAATTCTAAGGCGCTTGAGAGAACTCGG  GAGAAGGAACTCGGCAAATTGATACCGTAACTTCGGGAGAAGGTATGCCCTCTA  AGGTTAAGGACTTGCTCCGTAAGCCCCGGAGGGTCGCAGAGAATAGGTGGCTGC  GACTTGTTTATTAAAAACACGAGCACTCTTGCCAACACGAAAGTGGACGTATAG  GGTGTAACGCCTGCCCGGTGCCGGAAGGTTAATTGAAGATGTGCAAGCATCGGA  TCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAAT  TCCTTGTCGGGTAAGTTCCGACCCGCACGAATGGCGTAACGATGGCCACACTGTC  TCCTCCCGAGACTCAGCGAAGTTGAAGTGGTTGTGAAGATGCAATCTACCCGCTG  CTAGACGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGT  CACTTGTGTAGGATAGGTGGAAGGCTTGGAAGCAAAGACGCCAGTCTCTGTGGA  GTCGTCCTTGAAAATACCACCCTGGTGTCTTTGAGGTTCTAACCCAGACCCGTCA  TCCGGGTCGGGGACCGTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCA  AAGCGTAACGGAGGAGTTCGAAGGTTACCTAGGTCCGGTCGGAAATCGGACTGA  TAGTGCAATGGCAAAAGGTAGCTTAACTGCGAGACCGACAAGTCGGGCAGGTGC  GAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACG  GATAAAAGGTACTCCGGGGATAACAGGCTTGATTCCGCCCAAGAGTTCATATCG  ACGGCGGAGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGTAGTCGG  TCCCAAGGGTATGGCTGTTCGCCATTTTAAAGTGGTACGTGAGTTGGGTTTAAAA  CGTCGTGAGACAGTTTGGTCCCTATCTGCAGTGGGCGTTGGAAGTTTGACGGGGG  CTGCTCCTAGTACGAGAGGACCGGAGTGGACGAACCTCTGGTGTACCGGTTGTA  ACGCCAGTTGCATAGCCGGGTAGCTAAGTTCGGAAGAGATAAGCGCTGAAAGCA  TCTAAGCGCGAAACTCGCCTGAAGATGAGACTTCCCTTGCGGTTTAACCGCACTA  AAGGGTCGTTCGAGACCAGGACGTTGATAGGTGGGGTGTGGAAGCGCGGTAACG  CGTGAAGCTAACCCATACTAATTGCCCGTGAGGCTTGACTCT

RNA: SEQ ID NO: 325

UGAAAUGAUAGAGUCAAGUGAAUAAGUGCAUCAGGCGGAUGCCUUGGCGAUG  AUAGGCGACGAAGGACGUGUAAGCCUGCGAAAAGCGCGGGGGAGCUGGCAAU  AAAGCAAUGAUCCCGCGGUGUCCGAAUGGGGAAACCCACUGCAUUCUGUGCA  GUAUCCUAAGUUGAAUACAUAGGCUUAGAGAAGCGAACCCGGAGAACUGAAC  CAUCUAAGUACCCGGAGGAAAAGAAAUCAACCGAGAUUCCGCAAGUAGUGGC  GAGCGAACGCGGAGGAGCCUGUACGUAAUAACUGUCGAGGUAGAAGAACAAG  CUGGGAAGCUUGACCAUAGCGGGUGACAGUCCCGUAUUCGAAAUCUCAACAG  CGGUACUAAGCGUACGAAAAGUAGGGCGGGACACGUGAAAUCCUGUCUGAAU  AUGGGGGGACCAUCCUCCAAGGCUAAAUACUCAUCAUCGACCGAUAGUGAACC  AGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGUGAAACAGAAC  CUGAAACCUGAUGCAUACAAACAGUGGGAGCGCCCUAGUGGUGUGACUGCGU  ACCUUUUGUAUAAUGGGUCAACGACUUACAUUCAGUAGCGAGCUUAACCGGA  UAGGGGAGGCGUAGGGAAACCGAGUCUUAAUAGGGCGAUGAGUUGCUGGGUG  UAGACCCGAAACCGAGUGAUCUAUCCAUGGCCAGGUUGAAGGUGCCGUAACA  GGUACUGGAGGACCGAACCCACGCAUGUUGCAAAAUGCGGGGAUGAGCUGUG  GGUAGGGGUGAAAGGCUAAACAAACUCGGAGAUAGCUGGUUCUCCCCGAAAA  CUAUUUAGGUAGUGCCUCGAGCAAGACACUGAUGGGGGUAAAGCACUGUUAU  GGCUAGGGGGUUAUUGCAACUUACCAACCCAUGGCAAACUCAGAAUACCAUC  AAGUGGUUCCUCGGGAGACAGACAGCGGGUGCUAACGUCCGUUGUCAAGAGG  GAAACAACCCAGACCGCCGGCUAAGGUCCCAAAUGAUAGAUUAAGUGGUAAA  CGAAGUGGGAAGGCACAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAU  UUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGUCCUGCGCGGAAGAUGUA  ACGGGGCUCAAAUCUAUAACCGAAGCUGCGGAUGCCGGUUUACCGGCAUGGU  AGGGGAGCGUUCUGUAGGCUGAUGAAGGUGCAUUGUAAAGUGUGCUGGAGGU  AUCAGAAGUGCGAAUGUUGACAUGAGUAGCGAUAAAGCGGGUGAAAAGCCCG  CUCGCCGAAAGCCCAAGGUUUCCUACGCAACGUUCAUCGGCGUAGGGUGAGUC  GGCCCCUAAGGCGAGGCAGAAAUGCGUAGUCGAUGGGAAACAGGUUAAUAUU  CCUGUACUUGAUUCAAAUGCGAUGUGGGGACGGAGAAGGUUAGGUUGGCAAG  CUGUUGGAAUAGCUUGUUUAAGCCGGUAGGUGGAAGACUUAGGCAAAUCCGG  GUUUUCUUAACACCGAAGAAGUGAUGACGAGUGUUUACGGACACGAAGCAAC  CGAUACCACGCUUCCAGGAAAAGCCACUAAGCUUCAGUUUGAAUCGAACCGUA  CCGCAAACCGACACAGGUGGGCAGGAUGAGAAUUCUAAGGCGCUUGAGAGAA  CUCGGGAGAAGGAACUCGGCAAAUUGAUACCGUAACUUCGGGAGAAGGUAUG  CCCUCUAAGGUUAAGGACUUGCUCCGUAAGCCCCGGAGGGUCGCAGAGAAUA  GGUGGCUGCGACUUGUUUAUUAAAAACACGAGCACUCUUGCCAACACGAAAG  UGGACGUAUAGGGUGUAACGCCUGCCCGGUGCCGGAAGGUUAAUUGAAGAUG  UGCAAGCAUCGGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCC  UAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAUGGCGUA  ACGAUGGCCACACUGUCUCCUCCCGAGACUCAGCGAAGUUGAAGUGGUUGUG  AAGAUGCAAUCUACCCGCUGCUAGACGGAAAGACCCCGUGAACCUUUACUGUA  GCUUUGCAUUGGACUUUGAAGUCACUUGUGUAGGAUAGGUGGAAGGCUUGGA  AGCAAAGACGCCAGUCUCUGUGGAGUCGUCCUUGAAAAUACCACCCUGGUGUC  UUUGAGGUUCUAACCCAGACCCGUCAUCCGGGUCGGGGACCGUGCAUGGUAG  GCAGUUUGACUGGGGCGGUCUCCUCCCAAAGCGUAACGGAGGAGUUCGAAGG  UUACCUAGGUCCGGUCGGAAAUCGGACUGAUAGUGCAAUGGCAAAAGGUAGC  UUAACUGCGAGACCGACAAGUCGGGCAGGUGCGAAAGCAGGACAUAGUGAUC  CGGUGGUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGG  GAUAACAGGCUUGAUUCCGCCCAAGAGUUCAUAUCGACGGCGGAGUUUGGCA  CCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGUAGUCGGUCCCAAGGGUAUGG  CUGUUCGCCAUUUUAAAGUGGUACGUGAGUUGGGUUUAAAACGUCGUGAGAC  AGUUUGGUCCCUAUCUGCAGUGGGCGUUGGAAGUUUGACGGGGGCUGCUCCU  AGUACGAGAGGACCGGAGUGGACGAACCUCUGGUGUACCGGUUGUAACGCCA  GUUGCAUAGCCGGGUAGCUAAGUUCGGAAGAGAUAAGCGCUGAAAGCAUCUA  AGCGCGAAACUCGCCUGAAGAUGAGACUUCCCUUGCGGUUUAACCGCACUAAA  GGGUCGUUCGAGACCAGGACGUUGAUAGGUGGGGUGUGGAAGCGCGGUAACG  CGUGAAGCUAACCCAUACUAAUUGCCCGUGAGGCUUGACUCU

cDNA: SEQ ID NO: 326

AGAGTCAAGCCTCACGGGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTT  CCACACCCCACCTATCAACGTCCTGGTCTCGAACGACCCTTTAGTGCGGTTAAAC  CGCAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCGCTTAGATGCTTTCAGCGCTT  ATCTCTTCCGAACTTAGCTACCCGGCTATGCAACTGGCGTTACAACCGGTACACC  AGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCGTCAAACTTCC  AACGCCCACTGCAGATAGGGACCAAACTGTCTCACGACGTTTTAAACCCAACTC  ACGTACCACTTTAAAATGGCGAACAGCCATACCCTTGGGACCGACTACAGCCCC  AGGATGTGATGAGCCGACATCGAGGTGCCAAACTCCGCCGTCGATATGAACTCT  TGGGCGGAATCAAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGC  CCTTCCATACAGAACCACCGGATCACTATGTCCTGCTTTCGCACCTGCCCGACTT  GTCGGTCTCGCAGTTAAGCTACCTTTTGCCATTGCACTATCAGTCCGATTTCCGAC  CGGACCTAGGTAACCTTCGAACTCCTCCGTTACGCTTTGGGAGGAGACCGCCCCA  GTCAAACTGCCTACCATGCACGGTCCCCGACCCGGATGACGGGTCTGGGTTAGA  ACCTCAAAGACACCAGGGTGGTATTTTCAAGGACGACTCCACAGAGACTGGCGT  CTTTGCTTCCAAGCCTTCCACCTATCCTACACAAGTGACTTCAAAGTCCAATGCA  AAGCTACAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCAGCGGGTAGATTGCATC  TTCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCATCGTT  ACGCCATTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGA  CCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCCGATGCTTGCACATCT  TCAATTAACCTTCCGGCACCGGGCAGGCGTTACACCCTATACGTCCACTTTCGTG  TTGGCAAGAGTGCTCGTGTTTTTAATAAACAAGTCGCAGCCACCTATTCTCTGCG  ACCCTCCGGGGCTTACGGAGCAAGTCCTTAACCTTAGAGGGCATACCTTCTCCCG  AAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTA  GAATTCTCATCCTGCCCACCTGTGTCGGTTTGCGGTACGGTTCGATTCAAACTGA  AGCTTAGTGGCTTTTCCTGGAAGCGTGGTATCGGTTGCTTCGTGTCCGTAAACAC  TCGTCATCACTTCTTCGGTGTTAAGAAAACCCGGATTTGCCTAAGTCTTCCACCT  ACCGGCTTAAACAAGCTATTCCAACAGCTTGCCAACCTAACCTTCTCCGTCCCCA  CATCGCATTTGAATCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCAT  TTCTGCCTCGCCTTAGGGGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAA  ACCTTGGGCTTTCGGCGAGCGGGCTTTTCACCCGCTTTATCGCTACTCATGTCAA  CATTCGCACTTCTGATACCTCCAGCACACTTTACAATGCACCTTCATCAGCCTAC  AGAACGCTCCCCTACCATGCCGGTAAACCGGCATCCGCAGCTTCGGTTATAGATT  TGAGCCCCGTTACATCTTCCGCGCAGGACGACTCGACCAGTGAGCTATTACGCTT  TCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGTGCCTTCCCACT  TCGTTTACCACTTAATCTATCATTTGGGACCTTAGCCGGCGGTCTGGGTTGTTTCC  CTCTTGACAACGGACGTTAGCACCCGCTGTCTGTCTCCCGAGGAACCACTTGATG  GTATTCTGAGTTTGCCATGGGTTGGTAAGTTGCAATAACCCCCTAGCCATAACAG  TGCTTTACCCCCATCAGTGTCTTGCTCGAGGCACTACCTAAATAGTTTTCGGGGA  GAACCAGCTATCTCCGAGTTTGTTTAGCCTTTCACCCCTACCCACAGCTCATCCC  CGCATTTTGCAACATGCGTGGGTTCGGTCCTCCAGTACCTGTTACGGCACCTTCA  ACCTGGCCATGGATAGATCACTCGGTTTCGGGTCTACACCCAGCAACTCATCGCC  CTATTAAGACTCGGTTTCCCTACGCCTCCCCTATCCGGTTAAGCTCGCTACTGAA  TGTAAGTCGTTGACCCATTATACAAAAGGTACGCAGTCACACCACTAGGGCGCT  CCCACTGTTTGTATGCATCAGGTTTCAGGTTCTGTTTCACTCCCCTCCCGGGGTTC  TTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCGATGATGAGTATTTAGC  CTTGGAGGATGGTCCCCCCATATTCAGACAGGATTTCACGTGTCCCGCCCTACTT  TTCGTACGCTTAGTACCGCTGTTGAGATTTCGAATACGGGACTGTCACCCGCTAT  GGTCAAGCTTCCCAGCTTGTTCTTCTACCTCGACAGTTATTACGTACAGGCTCCTC  CGCGTTCGCTCGCCACTACTTGCGGAATCTCGGTTGATTTCTTTTCCTCCGGGTAC  TTAGATGGTTCAGTTCTCCGGGTTCGCTTCTCTAAGCCTATGTATTCAACTTAGGA  TACTGCACAGAATGCAGTGGGTTTCCCCATTCGGACACCGCGGGATCATTGCTTT  ATTGCCAGCTCCCCCGCGCTTTTCGCAGGCTTACACGTCCTTCGTCGCCTATCATC  GCCAAGGCATCCGCCTGATGCACTTATTCACTTGACTCTATCATTTCA

2. NGO_r03: NC_002946.2:c1121298-1119754

DNA (- strand): SEQ ID NO: 327

TGAACATAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACAC  ATGCAAGTCGGACGGCAGCACAGGGAAGCTTGCTTCTCGGGTGGCGAGTGGCGA  ACGGGTGAGTAACATATCGGAACGTACCGGGTAGCGGGGGATAACTGATCGAAA  GATCAGCTAATACCGCATACGTCTTGAGAGGGAAAGCAGGGGACCTTCGGGCCT  TGCGCTATCCGAGCGGCCGATATCTGATTAGCTGGTTGGCGGGGTAAAGGCCCA  CCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTG  AGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGG  CGCAAGCCTGATCCAGCCATGCCGCGTGTCTGAAGAAGGCCTTCGGGTTGTAAA  GGACTTTTGTCAGGGAAGAAAAGGCCGTTGCCAATATCGGCGGCCGATGACGGT  ACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG  GGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTT  AAGCAGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCTGAACTGGGT  GACTCGAGTGTGTCAGAGGGAGGTGGAATTCCACGTGTAGCAGTGAAATGCGTA  GAGATGTGGAGGAATACCGATGGCGAAGGCAGCCTCCTGGGATAACACTGACGT  TCATGTCCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGC  CCTAAACGATGTCAATTAGCTGTTGGGCAACTTGATTGCTTGGTAGCGTAGCTAA  CGCGTGAAATTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAA  TTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCG  AAGAACCTTACCTGGTTTTGACATGTGCGGAATCCTCCGGAGACGGAGGAGTGC  CTTCGGGAGCCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGA  TGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCATTCG  GTTGGGCACTCTAATGAGACTGCCGGTGACAAGCCGGAGGAAGGTGGGGATGAC  GTCAAGTCCTCATGGCCCTTATGACCAGGGCTTCACACGTCATACAATGGTCGGT  ACAGAGGGTAGCCAAGCCGCGAGGCGGAGCCAATCTCACAAAACCGATCGTAG  TCCGGATTGCACTCTGCAACTCGAGTGCATGAAGTCGGAATCGCTAGTAATCGCA  GGTCAGCATACTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACA  CCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAGTCCGCTT  ACCACGGTATGCTTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGA  ACCTGCGGCTGGATCACCTCCTTTCTA

RNA: SEQ ID NO: 328

UGAACAUAAGAGUUUGAUCCUGGCUCAGAUUGAACGCUGGCGGCAUGCUUUA  CACAUGCAAGUCGGACGGCAGCACAGGGAAGCUUGCUUCUCGGGUGGCGAGU  GGCGAACGGGUGAGUAACAUAUCGGAACGUACCGGGUAGCGGGGGAUAACUG  AUCGAAAGAUCAGCUAAUACCGCAUACGUCUUGAGAGGGAAAGCAGGGGACC  UUCGGGCCUUGCGCUAUCCGAGCGGCCGAUAUCUGAUUAGCUGGUUGGCGGG  GUAAAGGCCCACCAAGGCGACGAUCAGUAGCGGGUCUGAGAGGAUGAUCCGC  CACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAA  UUUUGGACAAUGGGCGCAAGCCUGAUCCAGCCAUGCCGCGUGUCUGAAGAAG  GCCUUCGGGUUGUAAAGGACUUUUGUCAGGGAAGAAAAGGCCGUUGCCAAUA  UCGGCGGCCGAUGACGGUACCUGAAGAAUAAGCACCGGCUAACUACGUGCCAG  CAGCCGCGGUAAUACGUAGGGUGCGAGCGUUAAUCGGAAUUACUGGGCGUAA  AGCGGGCGCAGACGGUUACUUAAGCAGGAUGUGAAAUCCCCGGGCUCAACCCG  GGAACUGCGUUCUGAACUGGGUGACUCGAGUGUGUCAGAGGGAGGUGGAAUU  CCACGUGUAGCAGUGAAAUGCGUAGAGAUGUGGAGGAAUACCGAUGGCGAAG  GCAGCCUCCUGGGAUAACACUGACGUUCAUGUCCGAAAGCGUGGGUAGCAAA  CAGGAUUAGAUACCCUGGUAGUCCACGCCCUAAACGAUGUCAAUUAGCUGUU  GGGCAACUUGAUUGCUUGGUAGCGUAGCUAACGCGUGAAAUUGACCGCCUGG  GGAGUACGGUCGCAAGAUUAAAACUCAAAGGAAUUGACGGGGACCCGCACAA  GCGGUGGAUGAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUU  UUGACAUGUGCGGAAUCCUCCGGAGACGGAGGAGUGCCUUCGGGAGCCGUAA  CACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAG  UCCCGCAACGAGCGCAACCCUUGUCAUUAGUUGCCAUCAUUCGGUUGGGCACU  CUAAUGAGACUGCCGGUGACAAGCCGGAGGAAGGUGGGGAUGACGUCAAGUC  CUCAUGGCCCUUAUGACCAGGGCUUCACACGUCAUACAAUGGUCGGUACAGAG  GGUAGCCAAGCCGCGAGGCGGAGCCAAUCUCACAAAACCGAUCGUAGUCCGGA  UUGCACUCUGCAACUCGAGUGCAUGAAGUCGGAAUCGCUAGUAAUCGCAGGU  CAGCAUACUGCGGUGAAUACGUUCCCGGGUCUUGUACACACCGCCCGUCACAC  CAUGGGAGUGGGGGAUACCAGAAGUAGGUAGGGUAACCGCAAGGAGUCCGCU  UACCACGGUAUGCUUCAUGACUGGGGUGAAGUCGUAACAAGGUAGCCGUAGG  GGAACCUGCGGCUGGAUCACCUCCUUUCUA

cDNA: SEQ ID NO: 329

TAGAAAGGAGGTGATCCAGCCGCAGGTTCCCCTACGGCTACCTTGTTACGACTTC  ACCCCAGTCATGAAGCATACCGTGGTAAGCGGACTCCTTGCGGTTACCCTACCTA  CTTCTGGTATCCCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGA  ACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCGACTTCATGCA  CTCGAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTGTGAGATTGGCTCCGC  CTCGCGGCTTGGCTACCCTCTGTACCGACCATTGTATGACGTGTGAAGCCCTGGT  CATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGCTTGTCACCGG  CAGTCTCATTAGAGTGCCCAACCGAATGATGGCAACTAATGACAAGGGTTGCGC  TCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCA  GCACCTGTGTTACGGCTCCCGAAGGCACTCCTCCGTCTCCGGAGGATTCCGCACA  TGTCAAAACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCACATCATCCAC  CGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCC  CAGGCGGTCAATTTCACGCGTTAGCTACGCTACCAAGCAATCAAGTTGCCCAAC  AGCTAATTGACATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCT  ACCCACGCTTTCGGACATGAACGTCAGTGTTATCCCAGGAGGCTGCCTTCGCCAT  CGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGAATTCCACCTCCC  TCTGACACACTCGAGTCACCCAGTTCAGAACGCAGTTCCCGGGTTGAGCCCGGG  GATTTCACATCCTGCTTAAGTAACCGTCTGCGCCCGCTTTACGCCCAGTAATTCC  GATTAACGCTCGCACCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGT  GCTTATTCTTCAGGTACCGTCATCGGCCGCCGATATTGGCAACGGCCTTTTCTTCC  CTGACAAAAGTCCTTTACAACCCGAAGGCCTTCTTCAGACACGCGGCATGGCTG  GATCAGGCTTGCGCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTC  TGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCTACTGAT  CGTCGCCTTGGTGGGCCTTTACCCCGCCAACCAGCTAATCAGATATCGGCCGCTC  GGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCCCTCTCAAGACGTATGCGGTAT  TAGCTGATCTTTCGATCAGTTATCCCCCGCTACCCGGTACGTTCCGATATGTTACT  CACCCGTTCGCCACTCGCCACCCGAGAAGCAAGCTTCCCTGTGCTGCCGTCCGAC  TTGCATGTGTAAAGCATGCCGCCAGCGTTCAATCTGAGCCAGGATCAAACTCTTA   TGTTCA

3. NGO_r05: NC_002946.2:c1261255-1258352

DNA (- strand): SEQ ID NO: 330

TGAAATGATAGAGTCAAGTGAATAAGTGCATCAGGCGGATGCCTTGGCGATGAT  AGGCGACGAAGGACGTGTAAGCCTGCGAAAAGCGCGGGGGAGCTGGCAATAAA  GCAATGATCCCGCGGTGTCCGAATGGGGAAACCCACTGCATTCTGTGCAGTATC  CTAAGTTGAATACATAGGCTTAGAGAAGCGAACCCGGAGAACTGAACCATCTAA  GTACCCGGAGGAAAAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAAC  GCGGAGGAGCCTGTACGTAATAACTGTCGAGGTAGAAGAACAAGCTGGGAAGCT  TGACCATAGCGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCG  TACGAAAAGTAGGGCGGGACACGTGAAATCCTGTCTGAATATGGGGGGACCATC  CTCCAAGGCTAAATACTCATCATCGACCGATAGTGAACCAGTACCGTGAGGGAA  AGGCGAAAAGAACCCCGGGAGGGGAGTGAAACAGAACCTGAAACCTGATGCAT  ACAAACAGTGGGAGCGCCCTAGTGGTGTGACTGCGTACCTTTTGTATAATGGGTC  AACGACTTACATTCAGTAGCGAGCTTAACCGGATAGGGGAGGCGTAGGGAAACC  GAGTCTTAATAGGGCGATGAGTTGCTGGGTGTAGACCCGAAACCGAGTGATCTA  TCCATGGCCAGGTTGAAGGTGCCGTAACAGGTACTGGAGGACCGAACCCACGCA  TGTTGCAAAATGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCTAAACAAACTC  GGAGATAGCTGGTTCTCCCCGAAAACTATTTAGGTAGTGCCTCGAGCAAGACAC  TGATGGGGGTAAAGCACTGTTATGGCTAGGGGGTTATTGCAACTTACCAACCCAT  GGCAAACTCAGAATACCATCAAGTGGTTCCTCGGGAGACAGACAGCGGGTGCTA  ACGTCCGTTGTCAAGAGGGAAACAACCCAGACCGCCGGCTAAGGTCCCAAATGA  TAGATTAAGTGGTAAACGAAGTGGGAAGGCACAGACAGCCAGGATGTTGGCTTA  GAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGTCCTGC  GCGGAAGATGTAACGGGGCTCAAATCTATAACCCAAGCTGCGTATGCCGGTTTA  CCGGCATGGTAGGGGAGCGTTCTGTAGGCTGATGAAGGTGCATTGTAAAGTGTG  CTGGAGGTATCAGAAGTGCGAATGTTGACATGAGTAGCGATAAAGCGGGTGAAA  AGCCCGCTCGCCGCAAAGCCCAAGGTTTCCTACGCAACGTTCATCGGCGTAGGG  TGAGTCGGCCCCTAAGGCGAGGCAGAAATGCGTAGTCGATGGGAAACAGGTTAA  TATTCCTGTACTTGATTCAAATGCGATGTGGGGACGGAGAAGGTTAGGTTGGCA  AGCTGTTGGAATAGCTTGTTTAAGCCGGTAGGTGGAAGACTTAGGCAAATCCGG  GTTTTCTTAACACCGAGAAGTGATGACGAGTGTCTACGGACACGAAGCAACCGA  TACCACGCTTCCAGGAAAAGCCACTAAGCTTCAGTTTGAATCGAACCGTACCGC  AAACCGACACAGGTGGGCAGGATGAGAATTCTAAGGCGCTTGAGAGAACTCGG  GAGAAGGAACTCGGCAAATTGATACCGTAACTTCGGGAGAAGGTATGCCCTCTA  AGGTTAAGGACTTGCTCCGTAAGCCCCGGAGGGTCGCAGAGAATAGGTGGCTGC  GACTGTTTATTAAAAACACAGCACTCTGCCAACACGAAAGTGGACGTATAGGGT  GTGACGCCTGCCCGGTGCCGGAAGGTTAATTGAAGATGTGCAAGCATCGGATCG  AAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCC  TTGTCGGGTAAGTTCCGACCCGCACGAATGGCGTAACGATGGCCACACTGTCTCC  TCCCGAGACTCAGCGAAGTTGAAGTGGTTGTGAAGATGCAATCTACCCGCTGCT  AGACGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGTCA  CTTGTGTAGGATAGGTGGGAGGCTTGGAAGCAGAGACGCCAGTCTCTGTGGAGT  CGTCCTTGAAATACCACCCTGGTGTCTTTGAGGTTCTAACCCAGACCCGTCATCC  GGGTCGGGGACCGTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAG  CGTAACGGAGGAGTTCGAAGGTTACCTAGGTCCGGTCGGAAATCGGACTGATAG  TGCAATGGCAAAAGGTAGCTTAACTGCGAGACCGACAAGTCGGGCAGGTGCGAA  AGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGAT  AAAAGGTACTCCGGGGATAACAGGCTGATTCCGCCCAAGAGTTCATATCGACGG  CGGAGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGTAGTCGGTCCC  AAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGTGAGCTGGGTTTAAAACGTCG  TGAGACAGTTTGGTCCCTATCTGCAGTGGGCGTTGGAAGTTTGACGGGGGCTGCT  CCTAGTACGAGAGGACCGGAGTGGACGAACCTCTGGTGTACCGGTTGTAACGCC  AGTTGCATAGCCGGGTAGCTAAGTTCGGAAGAGATAAGCGCTGAAAGCATCTAA  GCGCGAAACTCGCCTGAAGATGAGACTTCCCTTGCGGTTTAACCGCACTAAAGG  GTCGTTCGAGACCAGGACGTTGATAGGTGGGGTGTGGAAGCGCGGTAACGCGTG  AAGCTAACCCATACTAATTGCCCGTGAGGCTTGACTCT

RNA: SEQ ID NO: 331

UGAAAUGAUAGAGUCAAGUGAAUAAGUGCAUCAGGCGGAUGCCUUGGCGAUG  AUAGGCGACGAAGGACGUGUAAGCCUGCGAAAAGCGCGGGGGAGCUGGCAAU  AAAGCAAUGAUCCCGCGGUGUCCGAAUGGGGAAACCCACUGCAUUCUGUGCA  GUAUCCUAAGUUGAAUACAUAGGCUUAGAGAAGCGAACCCGGAGAACUGAAC  CAUCUAAGUACCCGGAGGAAAAGAAAUCAACCGAGAUUCCGCAAGUAGUGGC  GAGCGAACGCGGAGGAGCCUGUACGUAAUAACUGUCGAGGUAGAAGAACAAG  CUGGGAAGCUUGACCAUAGCGGGUGACAGUCCCGUAUUCGAAAUCUCAACAG  CGGUACUAAGCGUACGAAAAGUAGGGCGGGACACGUGAAAUCCUGUCUGAAU  AUGGGGGGACCAUCCUCCAAGGCUAAAUACUCAUCAUCGACCGAUAGUGAACC  AGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGUGAAACAGAAC  CUGAAACCUGAUGCAUACAAACAGUGGGAGCGCCCUAGUGGUGUGACUGCGU  ACCUUUUGUAUAAUGGGUCAACGACUUACAUUCAGUAGCGAGCUUAACCGGA  UAGGGGAGGCGUAGGGAAACCGAGUCUUAAUAGGGCGAUGAGUUGCUGGGUG  UAGACCCGAAACCGAGUGAUCUAUCCAUGGCCAGGUUGAAGGUGCCGUAACA  GGUACUGGAGGACCGAACCCACGCAUGUUGCAAAAUGCGGGGAUGAGCUGUG  GGUAGGGGUGAAAGGCUAAACAAACUCGGAGAUAGCUGGUUCUCCCCGAAAA  CUAUUUAGGUAGUGCCUCGAGCAAGACACUGAUGGGGGUAAAGCACUGUUAU  GGCUAGGGGGUUAUUGCAACUUACCAACCCAUGGCAAACUCAGAAUACCAUC  AAGUGGUUCCUCGGGAGACAGACAGCGGGUGCUAACGUCCGUUGUCAAGAGG  GAAACAACCCAGACCGCCGGCUAAGGUCCCAAAUGAUAGAUUAAGUGGUAAA  CGAAGUGGGAAGGCACAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAU  UUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGUCCUGCGCGGAAGAUGUA  ACGGGGCUCAAAUCUAUAACCCAAGCUGCGUAUGCCGGUUUACCGGCAUGGU  AGGGGAGCGUUCUGUAGGCUGAUGAAGGUGCAUUGUAAAGUGUGCUGGAGGU  AUCAGAAGUGCGAAUGUUGACAUGAGUAGCGAUAAAGCGGGUGAAAAGCCCG  CUCGCCGCAAAGCCCAAGGUUUCCUACGCAACGUUCAUCGGCGUAGGGUGAGU  CGGCCCCUAAGGCGAGGCAGAAAUGCGUAGUCGAUGGGAAACAGGUUAAUAU  UCCUGUACUUGAUUCAAAUGCGAUGUGGGGACGGAGAAGGUUAGGUUGGCAA  GCUGUUGGAAUAGCUUGUUUAAGCCGGUAGGUGGAAGACUUAGGCAAAUCCG  GGUUUUCUUAACACCGAGAAGUGAUGACGAGUGUCUACGGACACGAAGCAAC  CGAUACCACGCUUCCAGGAAAAGCCACUAAGCUUCAGUUUGAAUCGAACCGUA  CCGCAAACCGACACAGGUGGGCAGGAUGAGAAUUCUAAGGCGCUUGAGAGAA  CUCGGGAGAAGGAACUCGGCAAAUUGAUACCGUAACUUCGGGAGAAGGUAUG  CCCUCUAAGGUUAAGGACUUGCUCCGUAAGCCCCGGAGGGUCGCAGAGAAUA  GGUGGCUGCGACUGUUUAUUAAAAACACAGCACUCUGCCAACACGAAAGUGG  ACGUAUAGGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAAGAUGUGC  AAGCAUCGGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAA  GGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAUGGCGUAACG  AUGGCCACACUGUCUCCUCCCGAGACUCAGCGAAGUUGAAGUGGUUGUGAAG  AUGCAAUCUACCCGCUGCUAGACGGAAAGACCCCGUGAACCUUUACUGUAGCU  UUGCAUUGGACUUUGAAGUCACUUGUGUAGGAUAGGUGGGAGGCUUGGAAGC  AGAGACGCCAGUCUCUGUGGAGUCGUCCUUGAAAUACCACCCUGGUGUCUUU  GAGGUUCUAACCCAGACCCGUCAUCCGGGUCGGGGACCGUGCAUGGUAGGCAG  UUUGACUGGGGCGGUCUCCUCCCAAAGCGUAACGGAGGAGUUCGAAGGUUAC  CUAGGUCCGGUCGGAAAUCGGACUGAUAGUGCAAUGGCAAAAGGUAGCUUAA  CUGCGAGACCGACAAGUCGGGCAGGUGCGAAAGCAGGACAUAGUGAUCCGGU  GGUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUA  ACAGGCUGAUUCCGCCCAAGAGUUCAUAUCGACGGCGGAGUUUGGCACCUCGA  UGUCGGCUCAUCACAUCCUGGGGCUGUAGUCGGUCCCAAGGGUAUGGCUGUU  CGCCAUUUAAAGUGGUACGUGAGCUGGGUUUAAAACGUCGUGAGACAGUUUG  GUCCCUAUCUGCAGUGGGCGUUGGAAGUUUGACGGGGGCUGCUCCUAGUACG  AGAGGACCGGAGUGGACGAACCUCUGGUGUACCGGUUGUAACGCCAGUUGCA  UAGCCGGGUAGCUAAGUUCGGAAGAGAUAAGCGCUGAAAGCAUCUAAGCGCG  AAACUCGCCUGAAGAUGAGACUUCCCUUGCGGUUUAACCGCACUAAAGGGUC  GUUCGAGACCAGGACGUUGAUAGGUGGGGUGUGGAAGCGCGGUAACGCGUGA  AGCUAACCCAUACUAAUUGCCCGUGAGGCUUGACUCU

cDNA: SEQ ID NO: 332

AGAGTCAAGCCTCACGGGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTT  CCACACCCCACCTATCAACGTCCTGGTCTCGAACGACCCTTTAGTGCGGTTAAAC  CGCAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCGCTTAGATGCTTTCAGCGCTT  ATCTCTTCCGAACTTAGCTACCCGGCTATGCAACTGGCGTTACAACCGGTACACC  AGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCGTCAAACTTCC  AACGCCCACTGCAGATAGGGACCAAACTGTCTCACGACGTTTTAAACCCAGCTC  ACGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTACAGCCCCA  GGATGTGATGAGCCGACATCGAGGTGCCAAACTCCGCCGTCGATATGAACTCTT  GGGCGGAATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCC  TTCCATACAGAACCACCGGATCACTATGTCCTGCTTTCGCACCTGCCCGACTTGT  CGGTCTCGCAGTTAAGCTACCTTTTGCCATTGCACTATCAGTCCGATTTCCGACC  GGACCTAGGTAACCTTCGAACTCCTCCGTTACGCTTTGGGAGGAGACCGCCCCA  GTCAAACTGCCTACCATGCACGGTCCCCGACCCGGATGACGGGTCTGGGTTAGA  ACCTCAAAGACACCAGGGTGGTATTTCAAGGACGACTCCACAGAGACTGGCGTC  TCTGCTTCCAAGCCTCCCACCTATCCTACACAAGTGACTTCAAAGTCCAATGCAA  AGCTACAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCAGCGGGTAGATTGCATCT  TCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCATCGTTA  CGCCATTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACC  GTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCCGATGCTTGCACATCTTC  AATTAACCTTCCGGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTT  GGCAGAGTGCTGTGTTTTTAATAAACAGTCGCAGCCACCTATTCTCTGCGACCCT  CCGGGGCTTACGGAGCAAGTCCTTAACCTTAGAGGGCATACCTTCTCCCGAAGTT  ACGGTATCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAATTC  TCATCCTGCCCACCTGTGTCGGTTTGCGGTACGGTTCGATTCAAACTGAAGCTTA  GTGGCTTTTCCTGGAAGCGTGGTATCGGTTGCTTCGTGTCCGTAGACACTCGTCA  TCACTTCTCGGTGTTAAGAAAACCCGGATTTGCCTAAGTCTTCCACCTACCGGCT  TAAACAAGCTATTCCAACAGCTTGCCAACCTAACCTTCTCCGTCCCCACATCGCA  TTTGAATCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCATTTCTGCC  TCGCCTTAGGGGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTG  GGCTTTGCGGCGAGCGGGCTTTTCACCCGCTTTATCGCTACTCATGTCAACATTC  GCACTTCTGATACCTCCAGCACACTTTACAATGCACCTTCATCAGCCTACAGAAC  GCTCCCCTACCATGCCGGTAAACCGGCATACGCAGCTTGGGTTATAGATTTGAGC  CCCGTTACATCTTCCGCGCAGGACGACTCGACCAGTGAGCTATTACGCTTTCTTT  AAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGTGCCTTCCCACTTCGTT  TACCACTTAATCTATCATTTGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTT  GACAACGGACGTTAGCACCCGCTGTCTGTCTCCCGAGGAACCACTTGATGGTATT  CTGAGTTTGCCATGGGTTGGTAAGTTGCAATAACCCCCTAGCCATAACAGTGCTT  TACCCCCATCAGTGTCTTGCTCGAGGCACTACCTAAATAGTTTTCGGGGAGAACC  AGCTATCTCCGAGTTTGTTTAGCCTTTCACCCCTACCCACAGCTCATCCCCGCATT  TTGCAACATGCGTGGGTTCGGTCCTCCAGTACCTGTTACGGCACCTTCAACCTGG  CCATGGATAGATCACTCGGTTTCGGGTCTACACCCAGCAACTCATCGCCCTATTA  AGACTCGGTTTCCCTACGCCTCCCCTATCCGGTTAAGCTCGCTACTGAATGTAAG  TCGTTGACCCATTATACAAAAGGTACGCAGTCACACCACTAGGGCGCTCCCACT  GTTTGTATGCATCAGGTTTCAGGTTCTGTTTCACTCCCCTCCCGGGGTTCTTTTCG  CCTTTCCCTCACGGTACTGGTTCACTATCGGTCGATGATGAGTATTTAGCCTTGG  AGGATGGTCCCCCCATATTCAGACAGGATTTCACGTGTCCCGCCCTACTTTTCGT  ACGCTTAGTACCGCTGTTGAGATTTCGAATACGGGACTGTCACCCGCTATGGTCA  AGCTTCCCAGCTTGTTCTTCTACCTCGACAGTTATTACGTACAGGCTCCTCCGCGT  TCGCTCGCCACTACTTGCGGAATCTCGGTTGATTTCTTTTCCTCCGGGTACTTAGA  TGGTTCAGTTCTCCGGGTTCGCTTCTCTAAGCCTATGTATTCAACTTAGGATACTG  CACAGAATGCAGTGGGTTTCCCCATTCGGACACCGCGGGATCATTGCTTTATTGC  CAGCTCCCCCGCGCTTTTCGCAGGCTTACACGTCCTTCGTCGCCTATCATCGCCA  AGGCATCCGCCTGATGCACTTATTCACTTGACTCTATCATTTCA

4. NGO_r06: NC_002946.2:c1263390-1261846

DNA (- strand): SEQ ID NO: 333

TGAACATAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACAC  ATGCAAGTCGGACGGCAGCACAGGGAAGCTTGCTTCTCGGGTGGCGAGTGGCGA  ACGGGTGAGTAACATATCGGAACGTACCGGGTAGCGGGGGATAACTGATCGAAA  GATCAGCTAATACCGCATACGTCTTGAGAGGGAAAGCAGGGGACCTTCGGGCCT  TGCGCTATCCGAGCGGCCGATATCTGATTAGCTGGTTGGCGGGGTAAAGGCCCA  CCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTG  AGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGG  CGCAAGCCTGATCCAGCCATGCCGCGTGTCTGAAGAAGGCCTTCGGGTTGTAAA  GGACTTTTGTCAGGGAAGAAAAGGCCGTTGCCAATATCGGCGGCCGATGACGGT  ACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG  GGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTT  AAGCAGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCTGAACTGGGT  GACTCGAGTGTGTCAGAGGGAGGTGGAATTCCACGTGTAGCAGTGAAATGCGTA  GAGATGTGGAGGAATACCGATGGCGAAGGCAGCCTCCTGGGATAACACTGACGT  TCATGTCCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGC  CCTAAACGATGTCAATTAGCTGTTGGGCAACTTGATTGCTTGGTAGCGTAGCTAA  CGCGTGAAATTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAA  TTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCG  AAGAACCTTACCTGGTTTTGACATGTGCGGAATCCTCCGGAGACGGAGGAGTGC  CTTCGGGAGCCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGA  TGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCATTCG  GTTGGGCACTCTAATGAGACTGCCGGTGACAAGCCGGAGGAAGGTGGGGATGAC  GTCAAGTCCTCATGGCCCTTATGACCAGGGCTTCACACGTCATACAATGGTCGGT  ACAGAGGGTAGCCAAGCCGCGAGGCGGAGCCAATCTCACAAAACCGATCGTAG  TCCGGATTGCACTCTGCAACTCGAGTGCATGAAGTCGGAATCGCTAGTAATCGCA  GGTCAGCATACTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACA  CCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAGTCCGCTT  ACCACGGTATGCTTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGA  ACCTGCGGCTGGATCACCTCCTTTCTA

RNA: SEQ ID NO: 334

UGAACAUAAGAGUUUGAUCCUGGCUCAGAUUGAACGCUGGCGGCAUGCUUUA  CACAUGCAAGUCGGACGGCAGCACAGGGAAGCUUGCUUCUCGGGUGGCGAGU  GGCGAACGGGUGAGUAACAUAUCGGAACGUACCGGGUAGCGGGGGAUAACUG  AUCGAAAGAUCAGCUAAUACCGCAUACGUCUUGAGAGGGAAAGCAGGGGACC  UUCGGGCCUUGCGCUAUCCGAGCGGCCGAUAUCUGAUUAGCUGGUUGGCGGG  GUAAAGGCCCACCAAGGCGACGAUCAGUAGCGGGUCUGAGAGGAUGAUCCGC  CACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAA  UUUUGGACAAUGGGCGCAAGCCUGAUCCAGCCAUGCCGCGUGUCUGAAGAAG  GCCUUCGGGUUGUAAAGGACUUUUGUCAGGGAAGAAAAGGCCGUUGCCAAUA  UCGGCGGCCGAUGACGGUACCUGAAGAAUAAGCACCGGCUAACUACGUGCCAG  CAGCCGCGGUAAUACGUAGGGUGCGAGCGUUAAUCGGAAUUACUGGGCGUAA  AGCGGGCGCAGACGGUUACUUAAGCAGGAUGUGAAAUCCCCGGGCUCAACCCG  GGAACUGCGUUCUGAACUGGGUGACUCGAGUGUGUCAGAGGGAGGUGGAAUU  CCACGUGUAGCAGUGAAAUGCGUAGAGAUGUGGAGGAAUACCGAUGGCGAAG  GCAGCCUCCUGGGAUAACACUGACGUUCAUGUCCGAAAGCGUGGGUAGCAAA  CAGGAUUAGAUACCCUGGUAGUCCACGCCCUAAACGAUGUCAAUUAGCUGUU  GGGCAACUUGAUUGCUUGGUAGCGUAGCUAACGCGUGAAAUUGACCGCCUGG  GGAGUACGGUCGCAAGAUUAAAACUCAAAGGAAUUGACGGGGACCCGCACAA  GCGGUGGAUGAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUU  UUGACAUGUGCGGAAUCCUCCGGAGACGGAGGAGUGCCUUCGGGAGCCGUAA  CACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAG  UCCCGCAACGAGCGCAACCCUUGUCAUUAGUUGCCAUCAUUCGGUUGGGCACU  CUAAUGAGACUGCCGGUGACAAGCCGGAGGAAGGUGGGGAUGACGUCAAGUC  CUCAUGGCCCUUAUGACCAGGGCUUCACACGUCAUACAAUGGUCGGUACAGAG  GGUAGCCAAGCCGCGAGGCGGAGCCAAUCUCACAAAACCGAUCGUAGUCCGGA  UUGCACUCUGCAACUCGAGUGCAUGAAGUCGGAAUCGCUAGUAAUCGCAGGU  CAGCAUACUGCGGUGAAUACGUUCCCGGGUCUUGUACACACCGCCCGUCACAC  CAUGGGAGUGGGGGAUACCAGAAGUAGGUAGGGUAACCGCAAGGAGUCCGCU  UACCACGGUAUGCUUCAUGACUGGGGUGAAGUCGUAACAAGGUAGCCGUAGG  GGAACCUGCGGCUGGAUCACCUCCUUUCUA

cDNA: SEQ ID NO: 335

TAGAAAGGAGGTGATCCAGCCGCAGGTTCCCCTACGGCTACCTTGTTACGACTTC  ACCCCAGTCATGAAGCATACCGTGGTAAGCGGACTCCTTGCGGTTACCCTACCTA  CTTCTGGTATCCCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGA  ACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCGACTTCATGCA  CTCGAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTGTGAGATTGGCTCCGC  CTCGCGGCTTGGCTACCCTCTGTACCGACCATTGTATGACGTGTGAAGCCCTGGT  CATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGCTTGTCACCGG  CAGTCTCATTAGAGTGCCCAACCGAATGATGGCAACTAATGACAAGGGTTGCGC  TCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCA  GCACCTGTGTTACGGCTCCCGAAGGCACTCCTCCGTCTCCGGAGGATTCCGCACA  TGTCAAAACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCACATCATCCAC  CGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCC  CAGGCGGTCAATTTCACGCGTTAGCTACGCTACCAAGCAATCAAGTTGCCCAAC  AGCTAATTGACATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCT  ACCCACGCTTTCGGACATGAACGTCAGTGTTATCCCAGGAGGCTGCCTTCGCCAT  CGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGAATTCCACCTCCC  TCTGACACACTCGAGTCACCCAGTTCAGAACGCAGTTCCCGGGTTGAGCCCGGG  GATTTCACATCCTGCTTAAGTAACCGTCTGCGCCCGCTTTACGCCCAGTAATTCC  GATTAACGCTCGCACCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGT  GCTTATTCTTCAGGTACCGTCATCGGCCGCCGATATTGGCAACGGCCTTTTCTTCC  CTGACAAAAGTCCTTTACAACCCGAAGGCCTTCTTCAGACACGCGGCATGGCTG  GATCAGGCTTGCGCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTC  TGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCTACTGAT  CGTCGCCTTGGTGGGCCTTTACCCCGCCAACCAGCTAATCAGATATCGGCCGCTC  GGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCCCTCTCAAGACGTATGCGGTAT  TAGCTGATCTTTCGATCAGTTATCCCCCGCTACCCGGTACGTTCCGATATGTTACT  CACCCGTTCGCCACTCGCCACCCGAGAAGCAAGCTTCCCTGTGCTGCCGTCCGAC  TTGCATGTGTAAAGCATGCCGCCAGCGTTCAATCTGAGCCAGGATCAAACTCTTA   TGTTCA

5. NGO_r08: NC_002946.2:c1652830-1649928

DNA (- strand): SEQ ID NO: 336

TGAAATGATAGAGTCAAGTGAATAAGTGCATCAGGCGGATGCCTTGGCGATGAT  AGGCGACGAAGGACGTGTAAGCCTGCGAAAAGCGCGGGGGAGCTGGCAATAAA  GCAATGATCCCGCGGTGTCCGAATGGGGAAACCCACTGCATTCTGTGCAGTATC  CTAAGTTGAATACATAGGCTTAGAGAAGCGAACCCGGAGAACTGAACCATCTAA  GTACCCGGAGGAAAAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAAC  GCGGAGGAGCCTGTACGTAATAACTGTCGAGGTAGAAGAACAAGCTGGGAAGCT  TGACCATAGCGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCG  TACGAAAAGTAGGGCGGGACACGTGAAATCCTGTCTGAATATGGGGGGACCATC  CTCCAAGGCTAAATACTCATCATCGACCGATAGTGAACCAGTACCGTGAGGGAA  AGGCGAAAAGAACCCCGGGAGGGGAGTGAAACAGAACCTGAAACCTGATGCAT  ACAAACAGTGGGAGCGCCCTAGTGGTGTGACTGCGTACCTTTTGTATAATGGGTC  AACGACTTACATTCAGTAGCGAGCTTAACCGGATAGGGGAGGCGTAGGGAAACC  GAGTCTTAATAGGGCGATGAGTTGCTGGGTGTAGACCCGAAACCGAGTGATCTA  TCCATGGCCAGGTTGAAGGTGCCGTAACAGGTACTGGAGGACCGAACCCACGCA  TGTTGCAAAATGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCTAAACAAACTC  GGAGATAGCTGGTTCTCCCCGAAAACTATTTAGGTAGTGCCTCGAGCAAGACAC  TGATGGGGGTAAAGCACTGTTATGGCTAGGGGGTTATTGCAACTTACCAACCCAT  GGCAAACTCAGAATACCATCAAGTGGTTCCTCGGGAGACAGACAGCGGGTGCTA  ACGTCCGTTGTCAAGAGGGAAACAACCCAGACCGCCGGCTAAGGTCCCAAATGA  TAGATTAAGTGGTAAACGAAGTGGGAAGGCACAGACAGCCAGGATGTTGGCTTA  GAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGTCCTGC  GCGGAAGATGTAACGGGGCTCAAATCTATAACCGAAGCTGCGGATGCCGGTTTA  CCGGCATGGTAGGGGAGCGTTCTGTAGGCTGATGAAGGTGCATTGTAAAGTGTG  CTGGAGGTATCAGAAGTGCGAATGTTGACATGAGTAGCGATAAAGCGGGTGAAA  AGCCCGCTCGCCGAAAGCCCAAGGTTTCCTACGCAACGTTCATCGGCGTAGGGT  GAGTCGGCCCCTAAGGCGAGGCAGAAATGCGTAGTCGATGGGAAACAGGTTAAT  ATTCCTGTACTTGATTCAAATGCGATGTGGGGACGGAGAAGGTTAGGTTGGCAA  GCTGTTGGAATAGCTTGTTTAAGCCGGTAGGTGGAAGACTTAGGCAAATCCGGG  TTTTCTTAACACCGAGAAGTGATGACGAGTGTCTACGGACACGAAGCAACCGAT  ACCACGCTTCCAGGAAAAGCCACTAAGCTTCAGTTTGAATCGAACCGTACCGCA  AACCGACACAGGTGGGCAGGATGAGAATTCTAAGGCGCTTGAGAGAACTCGGG  AGAAGGAACTCGGCAAATTGATACCGTAACTTCGGGAGAAGGTATGCCCTCTAA  GGTTAAGGACTTGCTCCGTAAGCCCCGGAGGGTCGCAGAGAATAGGTGGCTGCG  ACTGTTTATTAAAAACACAGCACTCTGCCAACACGAAAGTGGACGTATAGGGTG  TGACGCCTGCCCGGTGCCGGAAGGTTAATTGAAGATGTGCAAGCATCGGATCGA  AGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCT  TGTCGGGTAAGTTCCGACCCGCACGAATGGCGTAACGATGGCCACACTGTCTCCT  CCCGAGACTCAGCGAAGTTGAAGTGGTTGTGAAGATGCAATCTACCCGCTGCTA  GACGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGTCAC  TTGTGTAGGATAGGTGGGAGGCTTGGAAGCAGAGACGCCAGTCTCTGTGGAGTC  GTCCTTGAAATACCACCCTGGTGTCTTTGAGGTTCTAACCCAGACCCGTCATCCG  GGTCGGGGACCGTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGC  GTAACGGAGGAGTTCGAAGGTTACCTAGGTCCGGTCGGAAATCGGACTGATAGT  GCAATGGCAAAAGGTAGCTTAACTGCGAGACCGACAAGTCGGGCAGGTGCGAA  AGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGAT  AAAAGGTACTCCGGGGATAACAGGCTGATTCCGCCCAAGAGTTCATATCGACGG  CGGAGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGTAGTCGGTCCC  AAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGTGAGCTGGGTTTAAAACGTCG  TGAGACAGTTTGGTCCCTATCTGCAGTGGGCGTTGGAAGTTTGACGGGGGCTGCT  CCTAGTACGAGAGGACCGGAGTGGACGAACCTCTGGTGTACCGGTTGTAACGCC  AGTTGCATAGCCGGGTAGCTAAGTTCGGAAGAGATAAGCGCTGAAAGCATCTAA  GCGCGAAACTCGCCTGAAGATGAGACTTCCCTTGCGGTTTAACCGCACTAAAGG  GTCGTTCGAGACCAGGACGTTGATAGGTGGGGTGTGGAAGCGCGGTAACGCGTG  AAGCTAACCCATACTAATTGCCCGTGAGGCTTGACTCT

RNA: SEQ ID NO: 337

UGAAAUGAUAGAGUCAAGUGAAUAAGUGCAUCAGGCGGAUGCCUUGGCGAUG  AUAGGCGACGAAGGACGUGUAAGCCUGCGAAAAGCGCGGGGGAGCUGGCAAU  AAAGCAAUGAUCCCGCGGUGUCCGAAUGGGGAAACCCACUGCAUUCUGUGCA  GUAUCCUAAGUUGAAUACAUAGGCUUAGAGAAGCGAACCCGGAGAACUGAAC  CAUCUAAGUACCCGGAGGAAAAGAAAUCAACCGAGAUUCCGCAAGUAGUGGC  GAGCGAACGCGGAGGAGCCUGUACGUAAUAACUGUCGAGGUAGAAGAACAAG  CUGGGAAGCUUGACCAUAGCGGGUGACAGUCCCGUAUUCGAAAUCUCAACAG  CGGUACUAAGCGUACGAAAAGUAGGGCGGGACACGUGAAAUCCUGUCUGAAU  AUGGGGGGACCAUCCUCCAAGGCUAAAUACUCAUCAUCGACCGAUAGUGAACC  AGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGUGAAACAGAAC  CUGAAACCUGAUGCAUACAAACAGUGGGAGCGCCCUAGUGGUGUGACUGCGU  ACCUUUUGUAUAAUGGGUCAACGACUUACAUUCAGUAGCGAGCUUAACCGGA  UAGGGGAGGCGUAGGGAAACCGAGUCUUAAUAGGGCGAUGAGUUGCUGGGUG  UAGACCCGAAACCGAGUGAUCUAUCCAUGGCCAGGUUGAAGGUGCCGUAACA  GGUACUGGAGGACCGAACCCACGCAUGUUGCAAAAUGCGGGGAUGAGCUGUG  GGUAGGGGUGAAAGGCUAAACAAACUCGGAGAUAGCUGGUUCUCCCCGAAAA  CUAUUUAGGUAGUGCCUCGAGCAAGACACUGAUGGGGGUAAAGCACUGUUAU  GGCUAGGGGGUUAUUGCAACUUACCAACCCAUGGCAAACUCAGAAUACCAUC  AAGUGGUUCCUCGGGAGACAGACAGCGGGUGCUAACGUCCGUUGUCAAGAGG  GAAACAACCCAGACCGCCGGCUAAGGUCCCAAAUGAUAGAUUAAGUGGUAAA  CGAAGUGGGAAGGCACAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAU  UUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGUCCUGCGCGGAAGAUGUA  ACGGGGCUCAAAUCUAUAACCGAAGCUGCGGAUGCCGGUUUACCGGCAUGGU  AGGGGAGCGUUCUGUAGGCUGAUGAAGGUGCAUUGUAAAGUGUGCUGGAGGU  AUCAGAAGUGCGAAUGUUGACAUGAGUAGCGAUAAAGCGGGUGAAAAGCCCG  CUCGCCGAAAGCCCAAGGUUUCCUACGCAACGUUCAUCGGCGUAGGGUGAGUC  GGCCCCUAAGGCGAGGCAGAAAUGCGUAGUCGAUGGGAAACAGGUUAAUAUU  CCUGUACUUGAUUCAAAUGCGAUGUGGGGACGGAGAAGGUUAGGUUGGCAAG  CUGUUGGAAUAGCUUGUUUAAGCCGGUAGGUGGAAGACUUAGGCAAAUCCGG  GUUUUCUUAACACCGAGAAGUGAUGACGAGUGUCUACGGACACGAAGCAACC  GAUACCACGCUUCCAGGAAAAGCCACUAAGCUUCAGUUUGAAUCGAACCGUAC  CGCAAACCGACACAGGUGGGCAGGAUGAGAAUUCUAAGGCGCUUGAGAGAAC  UCGGGAGAAGGAACUCGGCAAAUUGAUACCGUAACUUCGGGAGAAGGUAUGC  CCUCUAAGGUUAAGGACUUGCUCCGUAAGCCCCGGAGGGUCGCAGAGAAUAG  GUGGCUGCGACUGUUUAUUAAAAACACAGCACUCUGCCAACACGAAAGUGGA  CGUAUAGGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAAGAUGUGCA  AGCAUCGGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAG  GUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAUGGCGUAACGA  UGGCCACACUGUCUCCUCCCGAGACUCAGCGAAGUUGAAGUGGUUGUGAAGA  UGCAAUCUACCCGCUGCUAGACGGAAAGACCCCGUGAACCUUUACUGUAGCUU  UGCAUUGGACUUUGAAGUCACUUGUGUAGGAUAGGUGGGAGGCUUGGAAGCA  GAGACGCCAGUCUCUGUGGAGUCGUCCUUGAAAUACCACCCUGGUGUCUUUG  AGGUUCUAACCCAGACCCGUCAUCCGGGUCGGGGACCGUGCAUGGUAGGCAGU  UUGACUGGGGCGGUCUCCUCCCAAAGCGUAACGGAGGAGUUCGAAGGUUACC  UAGGUCCGGUCGGAAAUCGGACUGAUAGUGCAAUGGCAAAAGGUAGCUUAAC  UGCGAGACCGACAAGUCGGGCAGGUGCGAAAGCAGGACAUAGUGAUCCGGUG  GUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAA  CAGGCUGAUUCCGCCCAAGAGUUCAUAUCGACGGCGGAGUUUGGCACCUCGAU  GUCGGCUCAUCACAUCCUGGGGCUGUAGUCGGUCCCAAGGGUAUGGCUGUUC  GCCAUUUAAAGUGGUACGUGAGCUGGGUUUAAAACGUCGUGAGACAGUUUGG  UCCCUAUCUGCAGUGGGCGUUGGAAGUUUGACGGGGGCUGCUCCUAGUACGA  GAGGACCGGAGUGGACGAACCUCUGGUGUACCGGUUGUAACGCCAGUUGCAU  AGCCGGGUAGCUAAGUUCGGAAGAGAUAAGCGCUGAAAGCAUCUAAGCGCGA  AACUCGCCUGAAGAUGAGACUUCCCUUGCGGUUUAACCGCACUAAAGGGUCG  UUCGAGACCAGGACGUUGAUAGGUGGGGUGUGGAAGCGCGGUAACGCGUGAA  GCUAACCCAUACUAAUUGCCCGUGAGGCUUGACUCU

cDNA: SEQ ID NO: 338

AGAGTCAAGCCTCACGGGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTT  CCACACCCCACCTATCAACGTCCTGGTCTCGAACGACCCTTTAGTGCGGTTAAAC  CGCAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCGCTTAGATGCTTTCAGCGCTT  ATCTCTTCCGAACTTAGCTACCCGGCTATGCAACTGGCGTTACAACCGGTACACC  AGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCGTCAAACTTCC  AACGCCCACTGCAGATAGGGACCAAACTGTCTCACGACGTTTTAAACCCAGCTC  ACGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTACAGCCCCA  GGATGTGATGAGCCGACATCGAGGTGCCAAACTCCGCCGTCGATATGAACTCTT  GGGCGGAATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCC  TTCCATACAGAACCACCGGATCACTATGTCCTGCTTTCGCACCTGCCCGACTTGT  CGGTCTCGCAGTTAAGCTACCTTTTGCCATTGCACTATCAGTCCGATTTCCGACC  GGACCTAGGTAACCTTCGAACTCCTCCGTTACGCTTTGGGAGGAGACCGCCCCA  GTCAAACTGCCTACCATGCACGGTCCCCGACCCGGATGACGGGTCTGGGTTAGA  ACCTCAAAGACACCAGGGTGGTATTTCAAGGACGACTCCACAGAGACTGGCGTC  TCTGCTTCCAAGCCTCCCACCTATCCTACACAAGTGACTTCAAAGTCCAATGCAA  AGCTACAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCAGCGGGTAGATTGCATCT  TCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCATCGTTA  CGCCATTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACC  GTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCCGATGCTTGCACATCTTC  AATTAACCTTCCGGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTT  GGCAGAGTGCTGTGTTTTTAATAAACAGTCGCAGCCACCTATTCTCTGCGACCCT  CCGGGGCTTACGGAGCAAGTCCTTAACCTTAGAGGGCATACCTTCTCCCGAAGTT  ACGGTATCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAATTC  TCATCCTGCCCACCTGTGTCGGTTTGCGGTACGGTTCGATTCAAACTGAAGCTTA  GTGGCTTTTCCTGGAAGCGTGGTATCGGTTGCTTCGTGTCCGTAGACACTCGTCA  TCACTTCTCGGTGTTAAGAAAACCCGGATTTGCCTAAGTCTTCCACCTACCGGCT  TAAACAAGCTATTCCAACAGCTTGCCAACCTAACCTTCTCCGTCCCCACATCGCA  TTTGAATCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCATTTCTGCC  TCGCCTTAGGGGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTG  GGCTTTCGGCGAGCGGGCTTTTCACCCGCTTTATCGCTACTCATGTCAACATTCG  CACTTCTGATACCTCCAGCACACTTTACAATGCACCTTCATCAGCCTACAGAACG  CTCCCCTACCATGCCGGTAAACCGGCATCCGCAGCTTCGGTTATAGATTTGAGCC  CCGTTACATCTTCCGCGCAGGACGACTCGACCAGTGAGCTATTACGCTTTCTTTA  AATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGTGCCTTCCCACTTCGTTT  ACCACTTAATCTATCATTTGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTTG  ACAACGGACGTTAGCACCCGCTGTCTGTCTCCCGAGGAACCACTTGATGGTATTC  TGAGTTTGCCATGGGTTGGTAAGTTGCAATAACCCCCTAGCCATAACAGTGCTTT  ACCCCCATCAGTGTCTTGCTCGAGGCACTACCTAAATAGTTTTCGGGGAGAACCA  GCTATCTCCGAGTTTGTTTAGCCTTTCACCCCTACCCACAGCTCATCCCCGCATTT  TGCAACATGCGTGGGTTCGGTCCTCCAGTACCTGTTACGGCACCTTCAACCTGGC  CATGGATAGATCACTCGGTTTCGGGTCTACACCCAGCAACTCATCGCCCTATTAA  GACTCGGTTTCCCTACGCCTCCCCTATCCGGTTAAGCTCGCTACTGAATGTAAGT  CGTTGACCCATTATACAAAAGGTACGCAGTCACACCACTAGGGCGCTCCCACTG  TTTGTATGCATCAGGTTTCAGGTTCTGTTTCACTCCCCTCCCGGGGTTCTTTTCGC  CTTTCCCTCACGGTACTGGTTCACTATCGGTCGATGATGAGTATTTAGCCTTGGA  GGATGGTCCCCCCATATTCAGACAGGATTTCACGTGTCCCGCCCTACTTTTCGTA  CGCTTAGTACCGCTGTTGAGATTTCGAATACGGGACTGTCACCCGCTATGGTCAA  GCTTCCCAGCTTGTTCTTCTACCTCGACAGTTATTACGTACAGGCTCCTCCGCGTT  CGCTCGCCACTACTTGCGGAATCTCGGTTGATTTCTTTTCCTCCGGGTACTTAGAT  GGTTCAGTTCTCCGGGTTCGCTTCTCTAAGCCTATGTATTCAACTTAGGATACTGC  ACAGAATGCAGTGGGTTTCCCCATTCGGACACCGCGGGATCATTGCTTTATTGCC  AGCTCCCCCGCGCTTTTCGCAGGCTTACACGTCCTTCGTCGCCTATCATCGCCAA  GGCATCCGCCTGATGCACTTATTCACTTGACTCTATCATTTCA

6. NGO_r09: NC_002946.2:c1654965-1653421

DNA (- strand): SEQ ID NO: 339

TGAACATAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACAC  ATGCAAGTCGGACGGCAGCACAGGGAAGCTTGCTTCTCGGGTGGCGAGTGGCGA  ACGGGTGAGTAACATATCGGAACGTACCGGGTAGCGGGGGATAACTGATCGAAA  GATCAGCTAATACCGCATACGTCTTGAGAGGGAAAGCAGGGGACCTTCGGGCCT  TGCGCTATCCGAGCGGCCGATATCTGATTAGCTGGTTGGCGGGGTAAAGGCCCA  CCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTG  AGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGG  CGCAAGCCTGATCCAGCCATGCCGCGTGTCTGAAGAAGGCCTTCGGGTTGTAAA  GGACTTTTGTCAGGGAAGAAAAGGCCGTTGCCAATATCGGCGGCCGATGACGGT  ACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG  GGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTT  AAGCAGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCTGAACTGGGT  GACTCGAGTGTGTCAGAGGGAGGTGGAATTCCACGTGTAGCAGTGAAATGCGTA  GAGATGTGGAGGAATACCGATGGCGAAGGCAGCCTCCTGGGATAACACTGACGT  TCATGTCCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGC  CCTAAACGATGTCAATTAGCTGTTGGGCAACTTGATTGCTTGGTAGCGTAGCTAA  CGCGTGAAATTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAA  TTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCG  AAGAACCTTACCTGGTTTTGACATGTGCGGAATCCTCCGGAGACGGAGGAGTGC  CTTCGGGAGCCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGA  TGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCATTCG  GTTGGGCACTCTAATGAGACTGCCGGTGACAAGCCGGAGGAAGGTGGGGATGAC  GTCAAGTCCTCATGGCCCTTATGACCAGGGCTTCACACGTCATACAATGGTCGGT  ACAGAGGGTAGCCAAGCCGCGAGGCGGAGCCAATCTCACAAAACCGATCGTAG  TCCGGATTGCACTCTGCAACTCGAGTGCATGAAGTCGGAATCGCTAGTAATCGCA  GGTCAGCATACTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACA  CCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAGTCCGCTT  ACCACGGTATGCTTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGA  ACCTGCGGCTGGATCACCTCCTTTCTA

RNA: SEQ ID NO: 340

UGAACAUAAGAGUUUGAUCCUGGCUCAGAUUGAACGCUGGCGGCAUGCUUUA  CACAUGCAAGUCGGACGGCAGCACAGGGAAGCUUGCUUCUCGGGUGGCGAGU  GGCGAACGGGUGAGUAACAUAUCGGAACGUACCGGGUAGCGGGGGAUAACUG  AUCGAAAGAUCAGCUAAUACCGCAUACGUCUUGAGAGGGAAAGCAGGGGACC  UUCGGGCCUUGCGCUAUCCGAGCGGCCGAUAUCUGAUUAGCUGGUUGGCGGG  GUAAAGGCCCACCAAGGCGACGAUCAGUAGCGGGUCUGAGAGGAUGAUCCGC  CACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAA  UUUUGGACAAUGGGCGCAAGCCUGAUCCAGCCAUGCCGCGUGUCUGAAGAAG  GCCUUCGGGUUGUAAAGGACUUUUGUCAGGGAAGAAAAGGCCGUUGCCAAUA  UCGGCGGCCGAUGACGGUACCUGAAGAAUAAGCACCGGCUAACUACGUGCCAG  CAGCCGCGGUAAUACGUAGGGUGCGAGCGUUAAUCGGAAUUACUGGGCGUAA  AGCGGGCGCAGACGGUUACUUAAGCAGGAUGUGAAAUCCCCGGGCUCAACCCG  GGAACUGCGUUCUGAACUGGGUGACUCGAGUGUGUCAGAGGGAGGUGGAAUU  CCACGUGUAGCAGUGAAAUGCGUAGAGAUGUGGAGGAAUACCGAUGGCGAAG  GCAGCCUCCUGGGAUAACACUGACGUUCAUGUCCGAAAGCGUGGGUAGCAAA  CAGGAUUAGAUACCCUGGUAGUCCACGCCCUAAACGAUGUCAAUUAGCUGUU  GGGCAACUUGAUUGCUUGGUAGCGUAGCUAACGCGUGAAAUUGACCGCCUGG  GGAGUACGGUCGCAAGAUUAAAACUCAAAGGAAUUGACGGGGACCCGCACAA  GCGGUGGAUGAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUU  UUGACAUGUGCGGAAUCCUCCGGAGACGGAGGAGUGCCUUCGGGAGCCGUAA  CACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAG  UCCCGCAACGAGCGCAACCCUUGUCAUUAGUUGCCAUCAUUCGGUUGGGCACU  CUAAUGAGACUGCCGGUGACAAGCCGGAGGAAGGUGGGGAUGACGUCAAGUC  CUCAUGGCCCUUAUGACCAGGGCUUCACACGUCAUACAAUGGUCGGUACAGAG  GGUAGCCAAGCCGCGAGGCGGAGCCAAUCUCACAAAACCGAUCGUAGUCCGGA  UUGCACUCUGCAACUCGAGUGCAUGAAGUCGGAAUCGCUAGUAAUCGCAGGU  CAGCAUACUGCGGUGAAUACGUUCCCGGGUCUUGUACACACCGCCCGUCACAC  CAUGGGAGUGGGGGAUACCAGAAGUAGGUAGGGUAACCGCAAGGAGUCCGCU  UACCACGGUAUGCUUCAUGACUGGGGUGAAGUCGUAACAAGGUAGCCGUAGG  GGAACCUGCGGCUGGAUCACCUCCUUUCUA

cDNA: SEQ ID NO: 341

TAGAAAGGAGGTGATCCAGCCGCAGGTTCCCCTACGGCTACCTTGTTACGACTTC  ACCCCAGTCATGAAGCATACCGTGGTAAGCGGACTCCTTGCGGTTACCCTACCTA  CTTCTGGTATCCCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGA  ACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCGACTTCATGCA  CTCGAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTGTGAGATTGGCTCCGC  CTCGCGGCTTGGCTACCCTCTGTACCGACCATTGTATGACGTGTGAAGCCCTGGT  CATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGCTTGTCACCGG  CAGTCTCATTAGAGTGCCCAACCGAATGATGGCAACTAATGACAAGGGTTGCGC  TCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCA  GCACCTGTGTTACGGCTCCCGAAGGCACTCCTCCGTCTCCGGAGGATTCCGCACA  TGTCAAAACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCACATCATCCAC  CGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCC  CAGGCGGTCAATTTCACGCGTTAGCTACGCTACCAAGCAATCAAGTTGCCCAAC  AGCTAATTGACATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCT  ACCCACGCTTTCGGACATGAACGTCAGTGTTATCCCAGGAGGCTGCCTTCGCCAT  CGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGAATTCCACCTCCC  TCTGACACACTCGAGTCACCCAGTTCAGAACGCAGTTCCCGGGTTGAGCCCGGG  GATTTCACATCCTGCTTAAGTAACCGTCTGCGCCCGCTTTACGCCCAGTAATTCC  GATTAACGCTCGCACCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGT  GCTTATTCTTCAGGTACCGTCATCGGCCGCCGATATTGGCAACGGCCTTTTCTTCC  CTGACAAAAGTCCTTTACAACCCGAAGGCCTTCTTCAGACACGCGGCATGGCTG  GATCAGGCTTGCGCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTC  TGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCTACTGAT  CGTCGCCTTGGTGGGCCTTTACCCCGCCAACCAGCTAATCAGATATCGGCCGCTC  GGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCCCTCTCAAGACGTATGCGGTAT  TAGCTGATCTTTCGATCAGTTATCCCCCGCTACCCGGTACGTTCCGATATGTTACT  CACCCGTTCGCCACTCGCCACCCGAGAAGCAAGCTTCCCTGTGCTGCCGTCCGAC  TTGCATGTGTAAAGCATGCCGCCAGCGTTCAATCTGAGCCAGGATCAAACTCTTA   TGTTCA

7. NGO_r11: NC_002946.2:c1875982-1873080

DNA (- strand): SEQ ID NO: 342

TGAAATGATAGAGTCAAGTGAATAAGTGCATCAGGCGGATGCCTTGGCGATGAT  AGGCGACGAAGGACGTGTAAGCCTGCGAAAAGCGCGGGGGAGCTGGCAATAAA  GCAATGATCCCGCGGTGTCCGAATGGGGAAACCCACTGCATTCTGTGCAGTATC  CTAAGTTGAATACATAGGCTTAGAGAAGCGAACCCGGAGAACTGACCCATCTAA  GTACCCGGAGGAAAAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAAC  GCGGAGGAGCCTGTACGTAATAACTGTCGAGGTAGAAGAACAAGCTGGGAAGCT  TGACCATAGCGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCG  TACGAAAAGTAGGGCGGGACACGTGAAATCCTGTCTGAATATGGGGGGACCATC  CTCCAAGGCTAAATACTCATCATCGACCGATAGTGAACCAGTACCGTGAGGGAA  AGGCGAAAAGAACCCCGGGAGGGGAGTGAAACAGAACCTGAAACCTGATGCAT  ACAAACAGTGGGAGCGCCCTAGTGGTGTGACTGCGTACCTTTTGTATAATGGGTC  AACGACTTACATTCAGTAGCGAGCTTAACCGGATAGGGGAGGCGTAGGGAAACC  GAGTCTTAATAGGGCGATGAGTTGCTGGGTGTAGACCCGAAACCGAGTGATCTA  TCCATGGCCAGGTTGAAGGTGCCGTAACAGGTACTGGAGGACCGAACCCACGCA  TGTTGCAAAATGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCTAAACAAACTC  GGAGATAGCTGGTTCTCCCCGAAAACTATTTAGGTAGTGCCTCGAGCAAGACAC  TGATGGGGGTAAAGCACTGTTATGGCTAGGGGGTTATTGCAACTTACCAACCCAT  GGCAAACTCAGAATACCATCAAGTGGTTCCTCGGGAGACAGACAGCGGGTGCTA  ACGTCCGTTGTCAAGAGGGAAACAACCCAGACCGCCGGCTAAGGTCCCAAATGA  TAGATTAAGTGGTAAACGAAGTGGGAAGGCACAGACAGCCAGGATGTTGGCTTA  GAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGTCCTGC  GCGGAAGATGTAACGGGGCTCAAATCTATAACCGAAGCTGCGGATGCCGGTTTA  CCGGCATGGTAGGGGAGCGTTCTGTAGGCTGATGAAGGTGCATTGTAAAGTGTG  CTGGAGGTATCAGAAGTGCGAATGTTGACATGAGTAGCGATAAAGCGGGTGAAA  AGCCCGCTCGCCGAAAGCCCAAGGTTTCCTACGCAACGTTCATCGGCGTAGGGT  GAGTCGGCCCCTAAGGCGAGGCAGAAATGCGTAGTCGATGGGAAACAGGTTAAT  ATTCCTGTACTTGATTCAAATGCGATGTGGGGACGGAGAAGGTTAGGTTGGCAA  GCTGTTGGAATAGCTTGTTTAAGCCGGTAGGTGGAAGACTTAGGCAAATCCGGG  TTTTCTTAACACCGAGAAGTGATGACGAGTGTCTACGGACACGAAGCAACCGAT  ACCACGCTTCCAGGAAAAGCCACTAAGCTTCAGTTTGAATCGAACCGTACCGCA  AACCGACACAGGTGGGCAGGATGAGAATTCTAAGGCGCTTGAGAGAACTCGGG  AGAAGGAACTCGGCAAATTGATACCGTAACTTCGGGAGAAGGTATGCCCTCTAA  GGTTAAGGACTTGCTCCGTAAGCCCCGGAGGGTCGCAGAGAATAGGTGGCTGCG  ACTGTTTATTAAAAACACAGCACTCTGCCAACACGAAAGTGGACGTATAGGGTG  TGACGCCTGCCCGGTGCCGGAAGGTTAATTGAAGATGTGCAAGCATCGGATCGA  AGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCT  TGTCGGGTAAGTTCCGACCCGCACGAATGGCGTAACGATGGCCACACTGTCTCCT  CCCGAGACTCAGCGAAGTTGAAGTGGTTGTGAAGATGCAATCTACCCGCTGCTA  GACGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGTCAC  TTGTGTAGGATAGGTGGGAGGCTTGGAAGCAGAGACGCCAGTCTCTGTGGAGTC  GTCCTTGAAATACCACCCTGGTGTCTTTGAGGTTCTAACCCAGACCCGTCATCCG  GGTCGGGGACCGTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGC  GTAACGGAGGAGTTCGAAGGTTACCTAGGTCCGGTCGGAAATCGGACTGATAGT  GCAATGGCAAAAGGTAGCTTAACTGCGAGACCGACAAGTCGGGCAGGTGCGAA  AGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGAT  AAAAGGTACTCCGGGGATAACAGGCTGATTCCGCCCAAGAGTTCATATCGACGG  CGGAGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGTAGTCGGTCCC  AAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGTGAGCTGGGTTTAAAACGTCG  TGAGACAGTTTGGTCCCTATCTGCAGTGGGCGTTGGAAGTTTGACGGGGGCTGCT  CCTAGTACGAGAGGACCGGAGTGGACGAACCTCTGGTGTACCGGTTGTAACGCC  AGTTGCATAGCCGGGTAGCTAAGTTCGGAAGAGATAAGCGCTGAAAGCATCTAA  GCGCGAAACTCGCCTGAAGATGAGACTTCCCTTGCGGTTTAACCGCACTAAAGG  GTCGTTCGAGACCAGGACGTTGATAGGTGGGGTGTGGAAGCGCGGTAACGCGTG  AAGCTAACCCATACTAATTGCCCGTGAGGCTTGACTCT

RNA: SEQ ID NO: 343

UGAAAUGAUAGAGUCAAGUGAAUAAGUGCAUCAGGCGGAUGCCUUGGCGAUG  AUAGGCGACGAAGGACGUGUAAGCCUGCGAAAAGCGCGGGGGAGCUGGCAAU  AAAGCAAUGAUCCCGCGGUGUCCGAAUGGGGAAACCCACUGCAUUCUGUGCA  GUAUCCUAAGUUGAAUACAUAGGCUUAGAGAAGCGAACCCGGAGAACUGACC  CAUCUAAGUACCCGGAGGAAAAGAAAUCAACCGAGAUUCCGCAAGUAGUGGC  GAGCGAACGCGGAGGAGCCUGUACGUAAUAACUGUCGAGGUAGAAGAACAAG  CUGGGAAGCUUGACCAUAGCGGGUGACAGUCCCGUAUUCGAAAUCUCAACAG  CGGUACUAAGCGUACGAAAAGUAGGGCGGGACACGUGAAAUCCUGUCUGAAU  AUGGGGGGACCAUCCUCCAAGGCUAAAUACUCAUCAUCGACCGAUAGUGAACC  AGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGUGAAACAGAAC  CUGAAACCUGAUGCAUACAAACAGUGGGAGCGCCCUAGUGGUGUGACUGCGU  ACCUUUUGUAUAAUGGGUCAACGACUUACAUUCAGUAGCGAGCUUAACCGGA  UAGGGGAGGCGUAGGGAAACCGAGUCUUAAUAGGGCGAUGAGUUGCUGGGUG  UAGACCCGAAACCGAGUGAUCUAUCCAUGGCCAGGUUGAAGGUGCCGUAACA  GGUACUGGAGGACCGAACCCACGCAUGUUGCAAAAUGCGGGGAUGAGCUGUG  GGUAGGGGUGAAAGGCUAAACAAACUCGGAGAUAGCUGGUUCUCCCCGAAAA  CUAUUUAGGUAGUGCCUCGAGCAAGACACUGAUGGGGGUAAAGCACUGUUAU  GGCUAGGGGGUUAUUGCAACUUACCAACCCAUGGCAAACUCAGAAUACCAUC  AAGUGGUUCCUCGGGAGACAGACAGCGGGUGCUAACGUCCGUUGUCAAGAGG  GAAACAACCCAGACCGCCGGCUAAGGUCCCAAAUGAUAGAUUAAGUGGUAAA  CGAAGUGGGAAGGCACAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAU  UUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGUCCUGCGCGGAAGAUGUA  ACGGGGCUCAAAUCUAUAACCGAAGCUGCGGAUGCCGGUUUACCGGCAUGGU  AGGGGAGCGUUCUGUAGGCUGAUGAAGGUGCAUUGUAAAGUGUGCUGGAGGU  AUCAGAAGUGCGAAUGUUGACAUGAGUAGCGAUAAAGCGGGUGAAAAGCCCG  CUCGCCGAAAGCCCAAGGUUUCCUACGCAACGUUCAUCGGCGUAGGGUGAGUC  GGCCCCUAAGGCGAGGCAGAAAUGCGUAGUCGAUGGGAAACAGGUUAAUAUU  CCUGUACUUGAUUCAAAUGCGAUGUGGGGACGGAGAAGGUUAGGUUGGCAAG  CUGUUGGAAUAGCUUGUUUAAGCCGGUAGGUGGAAGACUUAGGCAAAUCCGG  GUUUUCUUAACACCGAGAAGUGAUGACGAGUGUCUACGGACACGAAGCAACC  GAUACCACGCUUCCAGGAAAAGCCACUAAGCUUCAGUUUGAAUCGAACCGUAC  CGCAAACCGACACAGGUGGGCAGGAUGAGAAUUCUAAGGCGCUUGAGAGAAC  UCGGGAGAAGGAACUCGGCAAAUUGAUACCGUAACUUCGGGAGAAGGUAUGC  CCUCUAAGGUUAAGGACUUGCUCCGUAAGCCCCGGAGGGUCGCAGAGAAUAG  GUGGCUGCGACUGUUUAUUAAAAACACAGCACUCUGCCAACACGAAAGUGGA  CGUAUAGGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAAGAUGUGCA  AGCAUCGGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAG  GUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAUGGCGUAACGA  UGGCCACACUGUCUCCUCCCGAGACUCAGCGAAGUUGAAGUGGUUGUGAAGA  UGCAAUCUACCCGCUGCUAGACGGAAAGACCCCGUGAACCUUUACUGUAGCUU  UGCAUUGGACUUUGAAGUCACUUGUGUAGGAUAGGUGGGAGGCUUGGAAGCA  GAGACGCCAGUCUCUGUGGAGUCGUCCUUGAAAUACCACCCUGGUGUCUUUG  AGGUUCUAACCCAGACCCGUCAUCCGGGUCGGGGACCGUGCAUGGUAGGCAGU  UUGACUGGGGCGGUCUCCUCCCAAAGCGUAACGGAGGAGUUCGAAGGUUACC  UAGGUCCGGUCGGAAAUCGGACUGAUAGUGCAAUGGCAAAAGGUAGCUUAAC  UGCGAGACCGACAAGUCGGGCAGGUGCGAAAGCAGGACAUAGUGAUCCGGUG  GUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAA  CAGGCUGAUUCCGCCCAAGAGUUCAUAUCGACGGCGGAGUUUGGCACCUCGAU  GUCGGCUCAUCACAUCCUGGGGCUGUAGUCGGUCCCAAGGGUAUGGCUGUUC  GCCAUUUAAAGUGGUACGUGAGCUGGGUUUAAAACGUCGUGAGACAGUUUGG  UCCCUAUCUGCAGUGGGCGUUGGAAGUUUGACGGGGGCUGCUCCUAGUACGA  GAGGACCGGAGUGGACGAACCUCUGGUGUACCGGUUGUAACGCCAGUUGCAU  AGCCGGGUAGCUAAGUUCGGAAGAGAUAAGCGCUGAAAGCAUCUAAGCGCGA  AACUCGCCUGAAGAUGAGACUUCCCUUGCGGUUUAACCGCACUAAAGGGUCG  UUCGAGACCAGGACGUUGAUAGGUGGGGUGUGGAAGCGCGGUAACGCGUGAA  GCUAACCCAUACUAAUUGCCCGUGAGGCUUGACUCU

cDNA: SEQ ID NO: 344

AGAGTCAAGCCTCACGGGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTT  CCACACCCCACCTATCAACGTCCTGGTCTCGAACGACCCTTTAGTGCGGTTAAAC  CGCAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCGCTTAGATGCTTTCAGCGCTT  ATCTCTTCCGAACTTAGCTACCCGGCTATGCAACTGGCGTTACAACCGGTACACC  AGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCGTCAAACTTCC  AACGCCCACTGCAGATAGGGACCAAACTGTCTCACGACGTTTTAAACCCAGCTC  ACGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTACAGCCCCA  GGATGTGATGAGCCGACATCGAGGTGCCAAACTCCGCCGTCGATATGAACTCTT  GGGCGGAATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCC  TTCCATACAGAACCACCGGATCACTATGTCCTGCTTTCGCACCTGCCCGACTTGT  CGGTCTCGCAGTTAAGCTACCTTTTGCCATTGCACTATCAGTCCGATTTCCGACC  GGACCTAGGTAACCTTCGAACTCCTCCGTTACGCTTTGGGAGGAGACCGCCCCA  GTCAAACTGCCTACCATGCACGGTCCCCGACCCGGATGACGGGTCTGGGTTAGA  ACCTCAAAGACACCAGGGTGGTATTTCAAGGACGACTCCACAGAGACTGGCGTC  TCTGCTTCCAAGCCTCCCACCTATCCTACACAAGTGACTTCAAAGTCCAATGCAA  AGCTACAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCAGCGGGTAGATTGCATCT  TCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCATCGTTA  CGCCATTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACC  GTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCCGATGCTTGCACATCTTC  AATTAACCTTCCGGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTT  GGCAGAGTGCTGTGTTTTTAATAAACAGTCGCAGCCACCTATTCTCTGCGACCCT  CCGGGGCTTACGGAGCAAGTCCTTAACCTTAGAGGGCATACCTTCTCCCGAAGTT  ACGGTATCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAATTC  TCATCCTGCCCACCTGTGTCGGTTTGCGGTACGGTTCGATTCAAACTGAAGCTTA  GTGGCTTTTCCTGGAAGCGTGGTATCGGTTGCTTCGTGTCCGTAGACACTCGTCA  TCACTTCTCGGTGTTAAGAAAACCCGGATTTGCCTAAGTCTTCCACCTACCGGCT  TAAACAAGCTATTCCAACAGCTTGCCAACCTAACCTTCTCCGTCCCCACATCGCA  TTTGAATCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCATTTCTGCC  TCGCCTTAGGGGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTG  GGCTTTCGGCGAGCGGGCTTTTCACCCGCTTTATCGCTACTCATGTCAACATTCG  CACTTCTGATACCTCCAGCACACTTTACAATGCACCTTCATCAGCCTACAGAACG  CTCCCCTACCATGCCGGTAAACCGGCATCCGCAGCTTCGGTTATAGATTTGAGCC  CCGTTACATCTTCCGCGCAGGACGACTCGACCAGTGAGCTATTACGCTTTCTTTA  AATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGTGCCTTCCCACTTCGTTT  ACCACTTAATCTATCATTTGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTTG  ACAACGGACGTTAGCACCCGCTGTCTGTCTCCCGAGGAACCACTTGATGGTATTC  TGAGTTTGCCATGGGTTGGTAAGTTGCAATAACCCCCTAGCCATAACAGTGCTTT  ACCCCCATCAGTGTCTTGCTCGAGGCACTACCTAAATAGTTTTCGGGGAGAACCA  GCTATCTCCGAGTTTGTTTAGCCTTTCACCCCTACCCACAGCTCATCCCCGCATTT  TGCAACATGCGTGGGTTCGGTCCTCCAGTACCTGTTACGGCACCTTCAACCTGGC  CATGGATAGATCACTCGGTTTCGGGTCTACACCCAGCAACTCATCGCCCTATTAA  GACTCGGTTTCCCTACGCCTCCCCTATCCGGTTAAGCTCGCTACTGAATGTAAGT  CGTTGACCCATTATACAAAAGGTACGCAGTCACACCACTAGGGCGCTCCCACTG  TTTGTATGCATCAGGTTTCAGGTTCTGTTTCACTCCCCTCCCGGGGTTCTTTTCGC  CTTTCCCTCACGGTACTGGTTCACTATCGGTCGATGATGAGTATTTAGCCTTGGA  GGATGGTCCCCCCATATTCAGACAGGATTTCACGTGTCCCGCCCTACTTTTCGTA  CGCTTAGTACCGCTGTTGAGATTTCGAATACGGGACTGTCACCCGCTATGGTCAA  GCTTCCCAGCTTGTTCTTCTACCTCGACAGTTATTACGTACAGGCTCCTCCGCGTT  CGCTCGCCACTACTTGCGGAATCTCGGTTGATTTCTTTTCCTCCGGGTACTTAGAT  GGGTCAGTTCTCCGGGTTCGCTTCTCTAAGCCTATGTATTCAACTTAGGATACTG  CACAGAATGCAGTGGGTTTCCCCATTCGGACACCGCGGGATCATTGCTTTATTGC  CAGCTCCCCCGCGCTTTTCGCAGGCTTACACGTCCTTCGTCGCCTATCATCGCCA  AGGCATCCGCCTGATGCACTTATTCACTTGACTCTATCATTTCA

8. NGO_r12: NC_002946.2:c1878117-1876573

DNA (- strand): SEQ ID NO: 10

TGAACATAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACAC  ATGCAAGTCGGACGGCAGCACAGGGAAGCTTGCTTCTCGGGTGGCGAGTGGCGA  ACGGGTGAGTAACATATCGGAACGTACCGGGTAGCGGGGGATAACTGATCGAAA  GATCAGCTAATACCGCATACGTCTTGAGAGGGAAAGCAGGGGACCTTCGGGCCT  TGCGCTATCCGAGCGGCCGATATCTGATTAGCTGGTTGGCGGGGTAAAGGCCCA  CCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTG  AGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGG  CGCAAGCCTGATCCAGCCATGCCGCGTGTCTGAAGAAGGCCTTCGGGTTGTAAA  GGACTTTTGTCAGGGAAGAAAAGGCCGTTGCCAATATCGGCGGCCGATGACGGT  ACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG  GGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTT  AAGCAGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCTGAACTGGGT  GACTCGAGTGTGTCAGAGGGAGGTGGAATTCCACGTGTAGCAGTGAAATGCGTA  GAGATGTGGAGGAATACCGATGGCGAAGGCAGCCTCCTGGGATAACACTGACGT  TCATGTCCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGC  CCTAAACGATGTCAATTAGCTGTTGGGCAACTTGATTGCTTGGTAGCGTAGCTAA  CGCGTGAAATTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAA  TTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCG  AAGAACCTTACCTGGTTTTGACATGTGCGGAATCCTCCGGAGACGGAGGAGTGC  CTTCGGGAGCCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGA  TGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCATTCG  GTTGGGCACTCTAATGAGACTGCCGGTGACAAGCCGGAGGAAGGTGGGGATGAC  GTCAAGTCCTCATGGCCCTTATGACCAGGGCTTCACACGTCATACAATGGTCGGT  ACAGAGGGTAGCCAAGCCGCGAGGCGGAGCCAATCTCACAAAACCGATCGTAG  TCCGGATTGCACTCTGCAACTCGAGTGCATGAAGTCGGAATCGCTAGTAATCGCA  GGTCAGCATACTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACA  CCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAGTCCGCTT  ACCACGGTATGCTTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGA  ACCTGCGGCTGGATCACCTCCTTTCTA

RNA: SEQ ID NO: 11

UGAACAUAAGAGUUUGAUCCUGGCUCAGAUUGAACGCUGGCGGCAUGCUUUA  CACAUGCAAGUCGGACGGCAGCACAGGGAAGCUUGCUUCUCGGGUGGCGAGU  GGCGAACGGGUGAGUAACAUAUCGGAACGUACCGGGUAGCGGGGGAUAACUG  AUCGAAAGAUCAGCUAAUACCGCAUACGUCUUGAGAGGGAAAGCAGGGGACC  UUCGGGCCUUGCGCUAUCCGAGCGGCCGAUAUCUGAUUAGCUGGUUGGCGGG  GUAAAGGCCCACCAAGGCGACGAUCAGUAGCGGGUCUGAGAGGAUGAUCCGC  CACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAA  UUUUGGACAAUGGGCGCAAGCCUGAUCCAGCCAUGCCGCGUGUCUGAAGAAG  GCCUUCGGGUUGUAAAGGACUUUUGUCAGGGAAGAAAAGGCCGUUGCCAAUA  UCGGCGGCCGAUGACGGUACCUGAAGAAUAAGCACCGGCUAACUACGUGCCAG  CAGCCGCGGUAAUACGUAGGGUGCGAGCGUUAAUCGGAAUUACUGGGCGUAA  AGCGGGCGCAGACGGUUACUUAAGCAGGAUGUGAAAUCCCCGGGCUCAACCCG  GGAACUGCGUUCUGAACUGGGUGACUCGAGUGUGUCAGAGGGAGGUGGAAUU  CCACGUGUAGCAGUGAAAUGCGUAGAGAUGUGGAGGAAUACCGAUGGCGAAG  GCAGCCUCCUGGGAUAACACUGACGUUCAUGUCCGAAAGCGUGGGUAGCAAA  CAGGAUUAGAUACCCUGGUAGUCCACGCCCUAAACGAUGUCAAUUAGCUGUU  GGGCAACUUGAUUGCUUGGUAGCGUAGCUAACGCGUGAAAUUGACCGCCUGG  GGAGUACGGUCGCAAGAUUAAAACUCAAAGGAAUUGACGGGGACCCGCACAA  GCGGUGGAUGAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUU  UUGACAUGUGCGGAAUCCUCCGGAGACGGAGGAGUGCCUUCGGGAGCCGUAA  CACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAG  UCCCGCAACGAGCGCAACCCUUGUCAUUAGUUGCCAUCAUUCGGUUGGGCACU  CUAAUGAGACUGCCGGUGACAAGCCGGAGGAAGGUGGGGAUGACGUCAAGUC  CUCAUGGCCCUUAUGACCAGGGCUUCACACGUCAUACAAUGGUCGGUACAGAG  GGUAGCCAAGCCGCGAGGCGGAGCCAAUCUCACAAAACCGAUCGUAGUCCGGA  UUGCACUCUGCAACUCGAGUGCAUGAAGUCGGAAUCGCUAGUAAUCGCAGGU  CAGCAUACUGCGGUGAAUACGUUCCCGGGUCUUGUACACACCGCCCGUCACAC  CAUGGGAGUGGGGGAUACCAGAAGUAGGUAGGGUAACCGCAAGGAGUCCGCU  UACCACGGUAUGCUUCAUGACUGGGGUGAAGUCGUAACAAGGUAGCCGUAGG  GGAACCUGCGGCUGGAUCACCUCCUUUCUA

cDNA: SEQ ID NO: 12

TAGAAAGGAGGTGATCCAGCCGCAGGTTCCCCTACGGCTACCTTGTTACGACTTC  ACCCCAGTCATGAAGCATACCGTGGTAAGCGGACTCCTTGCGGTTACCCTACCTA  CTTCTGGTATCCCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGA  ACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCGACTTCATGCA  CTCGAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTGTGAGATTGGCTCCGC  CTCGCGGCTTGGCTACCCTCTGTACCGACCATTGTATGACGTGTGAAGCCCTGGT  CATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGCTTGTCACCGG  CAGTCTCATTAGAGTGCCCAACCGAATGATGGCAACTAATGACAAGGGTTGCGC  TCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCA  GCACCTGTGTTACGGCTCCCGAAGGCACTCCTCCGTCTCCGGAGGATTCCGCACA  TGTCAAAACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCACATCATCCAC  CGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCC  CAGGCGGTCAATTTCACGCGTTAGCTACGCTACCAAGCAATCAAGTTGCCCAAC  AGCTAATTGACATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCT  ACCCACGCTTTCGGACATGAACGTCAGTGTTATCCCAGGAGGCTGCCTTCGCCAT  CGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGAATTCCACCTCCC  TCTGACACACTCGAGTCACCCAGTTCAGAACGCAGTTCCCGGGTTGAGCCCGGG  GATTTCACATCCTGCTTAAGTAACCGTCTGCGCCCGCTTTACGCCCAGTAATTCC  GATTAACGCTCGCACCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGT  GCTTATTCTTCAGGTACCGTCATCGGCCGCCGATATTGGCAACGGCCTTTTCTTCC  CTGACAAAAGTCCTTTACAACCCGAAGGCCTTCTTCAGACACGCGGCATGGCTG  GATCAGGCTTGCGCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTC  TGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCTACTGAT  CGTCGCCTTGGTGGGCCTTTACCCCGCCAACCAGCTAATCAGATATCGGCCGCTC  GGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCCCTCTCAAGACGTATGCGGTAT  TAGCTGATCTTTCGATCAGTTATCCCCCGCTACCCGGTACGTTCCGATATGTTACT  CACCCGTTCGCCACTCGCCACCCGAGAAGCAAGCTTCCCTGTGCTGCCGTCCGAC  TTGCATGTGTAAAGCATGCCGCCAGCGTTCAATCTGAGCCAGGATCAAACTCTTA   TGTTCA

1-52. (canceled)
 53. A method to detect a transcript of an N.gonorrhoeae, the method comprises quantitatively detecting a transcriptexpression value of an RNA marker of N. gonorrhoeae , in the N.gonorrhoeae following contacting of the N. gonorrhoeae with anantibiotic to obtain an antibiotic treated transcript expression valuefor the RNA marker of N. gonorrhoeae, wherein the RNA marker of N.gonorrhoeae is selected from: a transcript of N. gonorrhoeae gene havinglocus tag NGO0340, a transcript of N. gonorrhoeae gene having locus tagNGO1837, a transcript of N. gonorrhoeae gene having locus tag NGO1843, atranscript of N. gonorrhoeae gene having locus tag NGO2024, a transcriptof N. gonorrhoeae gene having locus tag NGO1845, a transcript of N.gonorrhoeae gene having locus tag NGO1677, a transcript of N.gonorrhoeae gene having locus tag NGO1844, a transcript of N.gonorrhoeae gene having locus tag NGO0171 , a transcript of N.gonorrhoeae gene having locus tag NGO1834, a transcript of N.gonorrhoeae gene having locus tag NGO0172, a transcript of N.gonorrhoeae gene having locus tag NGO1835, a transcript of N.gonorrhoeae gene having locus tag NGO1673, a transcript of N.gonorrhoeae gene having locus tag NGO1833, a transcript of N.gonorrhoeae gene having locus tag NGO2173, a transcript of N.gonorrhoeae gene having locus tag NGO0604, a transcript of N.gonorrhoeae gene having locus tag NGO0016, a transcript of N.gonorrhoeae gene having locus tag NGO2174, a transcript of N.gonorrhoeae gene having locus tag NGO2164, a transcript of N.gonorrhoeae gene having locus tag NGO1676, a transcript of N.gonorrhoeae gene having locus tag NGO1679, a transcript of N.gonorrhoeae gene having locus tag NGO1658, a transcript of N.gonorrhoeae gene having locus tag NGO1440, a transcript of N.gonorrhoeae gene having locus tag NGO0174, a transcript of N.gonorrhoeae gene having locus tag NGO0173, a transcript of N.gonorrhoeae gene having locus tag NGO0592, a transcript of N.gonorrhoeae gene having locus tag NGO1680, a transcript of N.gonorrhoeae gene having locus tag NGO0620, a transcript of N.gonorrhoeae gene having locus tag NGO1659, a transcript of N.gonorrhoeae gene having locus tag NGO1291, a transcript of N.gonorrhoeae gene having locus tag NGO0648, a transcript of N.gonorrhoeae gene having locus tag NGO0593, a transcript of N.gonorrhoeae gene having locus tag NGO1804, a transcript of N.gonorrhoeae gene having locus tag NGO0618, a transcript of N.gonorrhoeae gene having locus tag NGO0619, a transcript of N.gonorrhoeae gene having locus tag NGO1812, a transcript of N.gonorrhoeae gene having locus tag NGO1890, a transcript of N.gonorrhoeae gene having locus tag NGO2098, a transcript of N.gonorrhoeae gene having locus tag NGO2100, a transcript tRNA having aGenelD A9Y61 RS02445 or NGO t12, a transcript tRNA having a GenelD A9Y61RS04515 or NGO t15, a transcript tRNA having a GenelD A9Y61 RS04510 orNGO t14, a transcript tRNA having a GenelD A9Y61 RS09170 or NGO t37, atranscript tRNA having a GenelD A9Y61 RS00075 or NGO t01, and a sequencehaving at least 80% identity with any one of the transcripts.
 54. Themethod of claim 53, the method further comprising detecting whetherthere is a downshift in the transcript expression value of the RNAmarker of N. gonorrhoeae following the contacting by comparing theantibiotic treated transcript expression value with an untreated markerexpression value of the RNA marker of N. gonorrhoeae.
 55. The method ofclaim 54, wherein the reference expression value of the RNA marker of N.gonorrhoeae is a control transcript expression value obtained byquantitatively detecting the RNA of N. gonorrhoeae in a control sampleof the isolate or specimen not treated with the antibiotic.
 56. Themethod of claim 55, wherein the quantitatively detecting a transcriptexpression value of an RNA marker of N. gonorrhoeae is performed bycontacting a sample of an isolate or specimen comprising the N.gonorrhoeae with an antibiotic to obtain an antibiotic treated sample,quantitatively detecting a transcript expression value of a RNA markerof N. gonorrhoeae in the antibiotic treated sample, to provide anantibiotic treated transcript expression value for the RNA marker of N.gonorrhoeae, quantitatively detecting the transcript expression value ofthe RNA marker of N. gonorrhoeae in a control sample of the isolate orspecimen comprising the N. gonorrhoeae, to provide a control transcriptexpression value of the RNA marker of N. gonorrhoeae herein described;and detecting whether there is a downshift of the transcript of the RNAmarker of N. gonorrhoeae herein described in the treated sample withrespect to the control sample.
 57. The method of claim 54, furthercomprising normalizing the antibiotic treated transcript expressionvalue, the control transcript expression value and/or the related ratio,before detecting whether there is a downshift.
 58. The method of claim57, wherein the normalizing is performed with a reference measurementselected from expression value of a reference RNA, preferably a lowvariability and/or highly expressed RNA, DNA, number of cells, number ofsamples, effective amount of sample used and/or a related ratio.
 59. Themethod of claim 54, wherein the downshift of the transcript presence isat least 1.5-fold.
 60. The method of claim 54 , wherein the downshift ofthe transcript presence is at least 4-fold.
 61. The method of claim 54 ,wherein the downshift of the transcript presence is 6-fold or higher.62. The method of claim 54, wherein contacting the sample with anantibiotic is performed for up to 15 minutes.
 63. The method of claim54, wherein contacting the sample with an antibiotic is performed for upto 10 minutes.
 64. The method of claim 54, wherein contacting the samplewith an antibiotic is performed for up to 5 minutes.
 65. The method ofclaim 54, wherein the quantitatively detecting is performed by using aprobe specific for any one of the RNA markers and/or a correspondingcDNA marker and/or a probe specific for a cDNA marker corresponding toany one of the RNA markers.
 66. The method of claim 54, wherein thequantitatively detecting is performed in sample pretreated to enrich theRNA of N. gonorrhoeae and/or to remove of human RNA or RNA of othermicroorganisms in the sample. 67-124. (canceled)
 125. A system forperforming the method of claim 53, the system comprising a probespecific for an RNA marker, corresponding marker gene and/orcorresponding cDNA and reagents for detecting said probe, wherein theRNA marker is selected from a transcript of N. gonorrhoeae gene havinglocus tag NGO0340, a transcript of N. gonorrhoeae gene having locus tagNGO1837, a transcript of N. gonorrhoeae gene having locus tag NGO1843, atranscript of N. gonorrhoeae gene having locus tag NGO2024, a transcriptof N. gonorrhoeae gene having locus tag NGO1845, a transcript of N.gonorrhoeae gene having locus tag NGO1677, a transcript of N.gonorrhoeae gene having locus tag NGO1844, a transcript of N.gonorrhoeae gene having locus tag NGO0171, a transcript of N.gonorrhoeae gene having locus tag NGO1834, a transcript of N.gonorrhoeae gene having locus tag NGO0172, a transcript of N.gonorrhoeae gene having locus tag NGO1835, a transcript of N.gonorrhoeae gene having locus tag NGO1673, a transcript of N.gonorrhoeae gene having locus tag NGO1833, a transcript of N.gonorrhoeae gene having locus tag NGO2173, a transcript of N.gonorrhoeae gene having locus tag NGO0604, a transcript of N.gonorrhoeae gene having locus tag NGO0016, a transcript of N.gonorrhoeae gene having locus tag NGO2174, a transcript of N.gonorrhoeae gene having locus tag NGO2164, a transcript of N.gonorrhoeae gene having locus tag NGO1676, a transcript of N.gonorrhoeae gene having locus tag NGO1679, a transcript of N.gonorrhoeae gene having locus tag NGO1658, a transcript of N.gonorrhoeae gene having locus tag NGO1440, a transcript of N.gonorrhoeae gene having locus tag NGO0174, a transcript of N.gonorrhoeae gene having locus tag NGO0173, a transcript of N.gonorrhoeae gene having locus tag NGO0592, a transcript of N.gonorrhoeae gene having locus tag NGO1680, a transcript of N.gonorrhoeae gene having locus tag NGO0620, a transcript of N.gonorrhoeae gene having locus tag NGO1659, a transcript of N.gonorrhoeae gene having locus tag NGO1291 , a transcript of N.gonorrhoeae gene having locus tag NGO0648, a transcript of N.gonorrhoeae gene having locus tag NGO0593, a transcript of N.gonorrhoeae gene having locus tag NGO1804, a transcript of N.gonorrhoeae gene having locus tag NGO0618, a transcript of N.gonorrhoeae gene having locus tag NGO0619, a transcript of N.gonorrhoeae gene having locus tag NGO1812, a transcript of N.gonorrhoeae gene having locus tag NGO1890, a transcript of N.gonorrhoeae gene having locus tag NGO2098, a transcript of N.gonorrhoeae gene having locus tag NGO2100, a transcript tRNA having aGenelD A9Y61 RS02445 or NGO t12, a transcript tRNA having a GenelD A9Y61RS04515 or NGO t15, a transcript tRNA having a GenelD A9Y61 RS04510 orNGO t14, a transcript tRNA having a GenelD A9Y61 RS09170 or NGO t37, atranscript tRNA having a GenelD A9Y61 RS00075 or NGO t01 and a sequencehaving at least 80% identity with any one of the transcripts.
 126. Thesystem of claim 125, wherein the probe comprises a probe specific for atranscript selected from any one of the RNA markers and/or a cDNAcorresponding thereto, and a probe specific for a cDNA of any one of theRNA markers.
 127. The system of claim 125, wherein the system comprisesat least one probe specific for a transcript selected from N.gonorrhoeae gene having locus tag NGO1812, N. gonorrhoeae gene havinglocus tag NGO1680, N. gonorrhoeae gene having locus tag NGO1291, N.gonorrhoeae gene having locus tag NGO1673, N. gonorrhoeae gene havinglocus tag NGO0592, and N. gonorrhoeae gene having locus tag NGO0340 orfor a corresponding cDNA.
 128. The system of claim 125, wherein thesystem comprises at least one probe specific for a transcript selectedfrom N. gonorrhoeae gene having locus tag NGO1812, and/or N. gonorrhoeaegene having locus tag NGO1680 or for a corresponding cDNA.
 129. Thesystem of claim 125, wherein the probe comprises primers configured tospecifically hybridize with the transcript and/or the correspondingcDNA.
 130. The system of claim 129, wherein the system comprises a probespecific for a transcript of N. gonorrhoeae gene having locus tagNGO1812, the probe comprises a pair of primers having sequenceGCTACGATTCTCCCGAATTTGCC (SEQ ID NO: 160) (CCGCCKACCAAACGGTGAAC (SEQ IDNO: 161), a probe specific for a transcript of N. gonorrhoeae genehaving locus tag NGO1680, the probe comprises a pair of primers havingsequence TTGCCCAACTTGCAATCACG (SEQ ID NO: 162) and AGCACGCAAATCAGCCAATAC(SEQ ID NO: 163), a probe specific for a transcript of N. gonorrhoeaegene having locus tag NGO1291, the probe comprises a pair of primershaving sequence GCTTTGGAAAAAGCAGCCG (SEQ ID NO: 164) andGGTTTTGTTGTCGGTCAGGC (SEQ ID NO: 165), a probe specific for a transcriptof N. gonorrhoeae gene having locus tag NGO1673, the probe comprises apair of primers having sequence GACTTTTGCCGCTGCTTTG (SEQ ID NO: 166) andGCGCATTATTCGTGTGCAG (SEQ ID NO: 167), a probe specific for a transcriptof N. gonorrhoeae gene having locus tag NGO0592, the probe comprises apair of primers having sequence AAAGCCTTGGGTATTGCGG (SEQ ID NO: 168) andTGACCAAAGCAACCGGAAC (SEQ ID NO: 169), and/or a probe specific for atranscript of N. gonorrhoeae gene having locus tag NGO0340, the probecomprises a pair of primers having sequence GAGGCTTCCCCCGTATTGAG (SEQ IDNO: 170) and TTCAAAAGCCGCTTCGTTCG (SEQ ID NO: 171).